TY - CHAP AU - Hudhud, Lina AU - Szőke, Éva AU - Bencze, Noémi AU - Pohóczky, Krisztina AU - Helyes, Zsuzsanna ED - Szállási, Árpád TI - Transient Receptor Potential (TRP) channels in cancer: Implications for drug discovery and development T2 - TRP Channels as Therapeutic Targets : Advances in Basic Science and Clinical Use PB - Elsevier Academic Press CY - Waltham (MA) SN - 9780443186530 PY - 2024 SP - 387 EP - 401 PG - 15 DO - 10.1016/B978-0-443-18653-0.00003-4 UR - https://m2.mtmt.hu/api/publication/35196820 ID - 35196820 LA - English DB - MTMT ER - TY - CHAP AU - Kormos, Viktória AU - Kriszta, Gábor AU - Al-omari, Ammar AU - Kovács-Rozmer, Katalin AU - Konkoly, János AU - Pozsgai, Gábor AU - Pintér, Erika ED - Szállási, Árpád TI - TRP channels as potential target molecules for pharmacotherapy of neurological diseases T2 - TRP Channels as Therapeutic Targets : Advances in Basic Science and Clinical Use PB - Elsevier Academic Press CY - Waltham (MA) SN - 9780443186530 PY - 2024 SP - 421 EP - 455 PG - 35 DO - 10.1016/B978-0-443-18653-0.00011-3 UR - https://m2.mtmt.hu/api/publication/35184515 ID - 35184515 LA - English DB - MTMT ER - TY - JOUR AU - Göntér, K. AU - Dombi, Ágnes AU - Kormos, Viktória AU - Pintér, Erika AU - Pozsgai, Gábor TI - Examination of the Effect of Dimethyl Trisulfide in Acute Stress Mouse Model with the Potential Involvement of the TRPA1 Ion Channel JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 14 PG - 22 SN - 1661-6596 DO - 10.3390/ijms25147701 UR - https://m2.mtmt.hu/api/publication/35167289 ID - 35167289 N1 - Export Date: 22 August 2024 LA - English DB - MTMT ER - TY - JOUR AU - Borbély, Éva AU - Pethő, Gábor TI - Drug effects on neuropeptides and their receptors: Big hopes but moderate success in the treatment of chronic pain JF - CURRENT OPINION IN PHARMACOLOGY J2 - CURR OPIN PHARMACOL VL - 77 PY - 2024 PG - 8 SN - 1471-4892 DO - 10.1016/j.coph.2024.102474 UR - https://m2.mtmt.hu/api/publication/35166574 ID - 35166574 N1 - Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Szigeti Str. 12, Pécs, H-7624, Hungary Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus Str. 2, Pécs, H-7624, Hungary Centre for Neuroscience, University of Pécs, Ifjúság Str. 6, Pécs, H-7624, Hungary Export Date: 13 August 2024 CODEN: COPUB Correspondence Address: Borbély, É.; Department of Pharmacology and Pharmacotherapy, Szigeti Str. 12, Hungary; email: eva.borbely@aok.pte.hu LA - English DB - MTMT ER - TY - CONF AU - Faisal, Anna Zelma AU - Edina, Hornung AU - Técsi, László István AU - Botz, Lajos TI - Requirements for Clinical Data Assets Related to Medication for Comprehensive, Event History-Authentic Individualized Drug Therapies T2 - Congressus Pharmaceuticus Hungaricus XVII. and EUFEPS Annual Meeting 2024 PB - Magyar Gyógyszerésztudományi Társaság (MGYT) C1 - Budapest PY - 2024 SP - 58 EP - 58 PG - 1 UR - https://m2.mtmt.hu/api/publication/34915904 ID - 34915904 N1 - (Oral Presentations) LA - English DB - MTMT ER - TY - CONF AU - Dombi, Ágnes AU - Poór, Miklós TI - Interaction of Myricetin, Ampelopsin (Dihydromyricetin), and Their Sulfate Metabolites with Serum Albumin and Cytochrome P450 Enzymes T2 - Congressus Pharmaceuticus Hungaricus XVII. and EUFEPS Annual Meeting 2024 PB - Magyar Gyógyszerésztudományi Társaság (MGYT) C1 - Budapest PY - 2024 SP - 250 EP - 250 PG - 1 UR - https://m2.mtmt.hu/api/publication/34915605 ID - 34915605 N1 - (Poster Presentations) LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Ádám AU - Steib, Anita AU - Nehr-Majoros, Andrea Kinga AU - Kántás, Boglárka AU - Király, Ágnes AU - Racskó, Márk AU - Tóth, Balázs István AU - Szánti-Pintér, Eszter AU - Kudová, Eva AU - Skodáné Földes, Rita AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Anti-Nociceptive Effects of Sphingomyelinase and Methyl-Beta-Cyclodextrin in the Icilin-Induced Mouse Pain Model JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25094637 UR - https://m2.mtmt.hu/api/publication/34824919 ID - 34824919 N1 - cited By 0 AB - The thermo- and pain-sensitive Transient Receptor Potential Melastatin 3 and 8 (TRPM3 and TRPM8) ion channels are functionally associated in the lipid rafts of the plasma membrane. We have already described that cholesterol and sphingomyelin depletion, or inhibition of sphingolipid biosynthesis decreased the TRPM8 but not the TRPM3 channel opening on cultured sensory neurons. We aimed to test the effects of lipid raft disruptors on channel activation on TRPM3- and TRPM8-expressing HEK293T cells in vitro, as well as their potential analgesic actions in TRPM3 and TRPM8 channel activation involving acute pain models in mice. CHO cell viability was examined after lipid raft disruptor treatments and their effects on channel activation on channel expressing HEK293T cells by measurement of cytoplasmic Ca2+ concentration were monitored. The effects of treatments were investigated in Pregnenolone-Sulphate-CIM-0216-evoked and icilin-induced acute nocifensive pain models in mice. Cholesterol depletion decreased CHO cell viability. Sphingomyelinase and methyl-beta-cyclodextrin reduced the duration of icilin-evoked nocifensive behavior, while lipid raft disruptors did not inhibit the activity of recombinant TRPM3 and TRPM8. We conclude that depletion of sphingomyelin or cholesterol from rafts can modulate the function of native TRPM8 receptors. Furthermore, sphingolipid cleavage provided superiority over cholesterol depletion, and this method can open novel possibilities in the management of different pain conditions. LA - English DB - MTMT ER - TY - JOUR AU - Hudhud, Lina AU - Kovács-Rozmer, Katalin AU - Kecskés, Angéla AU - Pohóczky, Krisztina AU - Bencze, Noémi AU - Buzás, Krisztina AU - Szőke, Éva AU - Helyes, Zsuzsanna TI - Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25073760 UR - https://m2.mtmt.hu/api/publication/34797924 ID - 34797924 N1 - * Megosztott szerzőség AB - Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives. LA - English DB - MTMT ER - TY - JOUR AU - Poór, Miklós AU - Dombi, Ágnes AU - Fliszár-Nyúl, Eszter AU - Pedroni, Lorenzo AU - Dellafiora, Luca TI - Effects of Chrysin and Chrysin-7-sulfate on Ochratoxin A-Albumin Interactions and on the Plasma and Kidney Levels of the Mycotoxin in Rats JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 15 SP - 17655 EP - 17666 PG - 12 SN - 2470-1343 DO - 10.1021/acsomega.4c01738 UR - https://m2.mtmt.hu/api/publication/34766036 ID - 34766036 N1 - Department of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs, H-7624, Hungary Molecular Medicine Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, H-7624, Hungary Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Rókus u. 2, Pécs, H-7624, Hungary Department of Food and Drug, University of Parma, Via G.P. Usberti 27/A, Parma, 43124, Italy Export Date: 3 May 2024 Correspondence Address: Poór, M.; Department of Laboratory Medicine, Ifjúság útja 13, Hungary; email: poor.miklos@pte.hu LA - English DB - MTMT ER - TY - JOUR AU - Hana, Kaci AU - Bakos, Éva AU - Needs, Paul W. AU - Kroon, Paul A. AU - Valentová, Kateřina AU - Poór, Miklós AU - Laczka, Csilla TI - The 2-aminoethyl diphenylborinate-based fluorescent method identifies quercetin and luteolin metabolites as substrates of Organic anion transporting polypeptides, OATP1B1 and OATP2B1 JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 196 PY - 2024 PG - 10 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106740 UR - https://m2.mtmt.hu/api/publication/34719089 ID - 34719089 AB - Organic anion transporting polypeptides (OATPs), OATP1B1 and OATP2B1 are membrane proteins mediating the cellular uptake of chemically diverse organic compounds. OATP1B1 is exclusively expressed in hepatocytes and plays a key role in hepatic detoxification. The ubiquitously expressed OATP2B1 promotes the intestinal absorption of orally administered drugs. Flavonoids are widely found in foods and beverages, and many of them can inhibit OATP function, resulting in food-drug interactions. In our previous work, we have shown that not only luteolin (LUT) and quercetin (Q), but also some of their metabolites can inhibit OATP1B1 and OATP2B1 activity. However, data about the potential direct transport of these flavonoids by OATPs have been incomplete. Hence, in the current study, we developed a simple, fluorescence-based method for the measurement of intracellular flavonoid levels. The method applies a cell-permeable small molecule (2-aminoethyl diphenylborinate, 2-APB), that, upon forming a complex with flavonoids, results in their fluorescence enhancement. This way the direct uptake of LUT and Q, and also their metabolites could be investigated both by confocal microscopy and in a fluorescence plate reader in living cells. With this approach we identified quercetin-3'-O-sulfate, luteolin-3'-O-glucuronide, luteolin-7-O-glucuronide and luteolin-3'-O-sulfate as substrates of both OATP1B1 and OATP2B1. Our results highlight that OATP1B1 and OATP2B1 can be key participants in the transmembrane movement of cell-permeable LUT and Q conjugates with otherwise low cell permeability. In addition, the novel method developed in this study can be a good completion to existing fluorescence-based assays to investigate OATP function. LA - English DB - MTMT ER -