TY - JOUR AU - Bovári-Biri, Judit AU - Garai, Kitti AU - Lovászi, Borbála AU - Veréb, Zoltán AU - Pongrácz, Judit TI - Miniature modeling while aiming for transplantation: Current challenges and future perspectives of lung bioprinting JF - INTERNATIONAL JOURNAL OF BIOPRINTING J2 - INT J BIOPRINT VL - 11 PY - 2025 IS - 1 SP - 30 EP - 56 PG - 27 SN - 2424-7723 DO - 10.36922/ijb.4673 UR - https://m2.mtmt.hu/api/publication/35501164 ID - 35501164 N1 - Export Date: 12 March 2025 AB - The development of three-dimensional (3D) lung organs or tissues using gravitational methods and bioprinting technologies shows great promise for producing lung tissue for research, pharmaceutical, and clinical applications. The advancement of innovative technologies can improve our understanding of lung diseases and, if necessary, enable the production of replacement lungs for transplantation. The development of functional organs-on-a-chip and disease-specific lung tissues could provide a deep understanding of the molecular mechanisms underlying lung diseases and aid the identification of drug targets. This knowledge has the potential to enhance our understanding of lung tissue regeneration processes and could lead to the development of more effective treatments for human lung diseases. So much so that lung transplants in most disease-induced cases would not be necessary, as the appropriate medications could induce regeneration of the damaged organ. This review highlights the importance of using a variety of materials, preparation methods, and sizes of lung tissues in 3D bioprinting technologies to understand lung function better, test for drug selection during therapy and eventually produce transplantable organs, if necessary. The review also emphasizes the need for improvements in legislation and guidelines for researchers aiming to achieve quality-assured biomanufacturing. LA - English DB - MTMT ER - TY - JOUR AU - Bovári-Biri, Judit AU - Miskei, Judith Anna AU - Kövér, Zsanett AU - Steinerbrunnerné Nagy, Alexandra AU - Kardos, Kinga AU - Maróti, Péter AU - Pongrácz, Judit TI - Advancements in Bone Replacement Techniques–Potential Uses After Maxillary and Mandibular Resections Due to Medication-Related Osteonecrosis of the Jaw (MRONJ) JF - CELLS J2 - CELLS-BASEL VL - 14 PY - 2025 IS - 2 PG - 25 SN - 2073-4409 DO - 10.3390/cells14020145 UR - https://m2.mtmt.hu/api/publication/35707886 ID - 35707886 AB - Maxillofacial bone defects can have a profound impact on both facial function and aesthetics. While various biomaterial scaffolds have shown promise in addressing these challenges, regenerating bone in this region remains complex due to its irregular shape, intricate structure, and differing cellular origins compared to other bones in the human body. Moreover, the significant and variable mechanical loads placed on the maxillofacial bones add further complexity, especially in cases of difficult-to-treat medical conditions. This review provides a brief overview of medication-related osteonecrosis of the jaw (MRONJ), highlighting the medication-induced adverse reactions and the associated clinical challenges in treating this condition. The purpose of this manuscript is to emphasize the role of biotechnology and tissue engineering technologies in therapy. By using scaffold materials and biofactors in combination with autologous cells, innovative solutions are explored for the repair of damaged facial bones. The ongoing search for effective scaffolds that can address these challenges and improve in vitro bone preparation for subsequent regeneration in the maxillofacial region remains critical. The primary purpose of this review is to spotlight current research trends and novel approaches in this area. LA - English DB - MTMT ER - TY - JOUR AU - Ou, Hairui AU - Ádám, Zoltán Mihály AU - Csuth, Tamás Imre AU - Kovács-Öller, Tamás AU - Sebők-Tornai, Abigél AU - Czömpöly, Tamás AU - Kvell, Krisztián TI - The potential role of pooled bovine milk-derived EVs in regulating epithelial cells through human primary macrophages JF - FOOD BIOSCIENCE J2 - FOOD BIOSCI VL - 65 PY - 2025 PG - 13 SN - 2212-4292 DO - 10.1016/j.fbio.2025.106011 UR - https://m2.mtmt.hu/api/publication/35764995 ID - 35764995 N1 - Funding Agency and Grant Number: China Scholarship Council-Hungary Stipendium Hungaricum Scholarship Program - University of Pecs [013_2023_PTE_RK/3]; National Laboratory of Virology at the Szentagothai Research Center of the University of Pecs Funding text: H.O. is the recipient of a PhD research fellowship from the China Scholarship Council-Hungary Stipendium Hungaricum Scholarship Program. This research was funded by the University of Pecs, grant number 013_2023_PTE_RK/3. The research was performed in collabo-ration with the Histology and Light Microscopy core facility and the National Laboratory of Virology at the Szentagothai Research Center of the University of Pecs. The research was performed in collaboration with the Central Electron Microscope Laboratory of the University of Pecs. Raw data have ben uploaded to the GEO repository (GSE 286929, GSE 286933, GSE 286937. LA - English DB - MTMT ER - TY - JOUR AU - Azimi, S. AU - Merza, M.S. AU - Ghasemi, F. AU - Dhahi, H.A. AU - Baradarbarjastehbaf, Farid AU - Moosavi, M. AU - Kargar, P.G. AU - Len, C. TI - Retraction notice to “Green and rapid and instrumental one-pot method for the synthesis of imidazolines having potential anti-SARS-CoV-2 main protease activity” [Sustain. Chem. Pharm. 34 (2023) 101136] (Sustainable Chemistry and Pharmacy (2023) 34, (S2352554123001705), (10.1016/j.scp.2023.101136)) JF - SUSTAINABLE CHEMISTRY AND PHARMACY J2 - SUSTAIN CHEM PHARM VL - 44 PY - 2025 SN - 2352-5541 DO - 10.1016/j.scp.2025.101920 UR - https://m2.mtmt.hu/api/publication/35765010 ID - 35765010 LA - English DB - MTMT ER - TY - CONF AU - Csala, Dávid AU - Ádám, Zoltán AU - Horváth-Szalai, Zoltán AU - Sebesi, Balázs AU - Garai, Kitti AU - Kvell, Krisztián AU - Wilhelm, Márta ED - Béres, András TI - A tápanyagidőzítés és krónikus rezisztenciaedzés moduláló hatása az extracelluláris vezikulák miRNS-profiljára T2 - SZOFIKON 2025 Szentágothai Felsőoktatási Interdiszciplináris Konferencia: Absztraktkötet PB - PTE TTK Szentágothai János Protestáns Szakkollégium C1 - Pécs SN - 9789636263874 PY - 2025 SP - 30 EP - 30 PG - 1 UR - https://m2.mtmt.hu/api/publication/36062607 ID - 36062607 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Steib, Anita AU - Kovács-Rozmer, Katalin AU - Szőke, Éva AU - Kun, József AU - Borbásné Farkas, Kornélia AU - Feller, Diána AU - Pongrácz, Judit AU - Pohóczky, Krisztina AU - Helyes, Zsuzsanna TI - The TRPA1 cation channel is upregulated by cigarette smoke in mouse and human macrophages modulating lung inflammation. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 15 PY - 2025 IS - 1 PG - 15 SN - 2045-2322 DO - 10.1038/s41598-025-95662-y UR - https://m2.mtmt.hu/api/publication/36067444 ID - 36067444 N1 - Krisztina Pohóczky and Zsuzsanna Helyes contributed equally to the paper as last authors. AB - Cigarette smoke (CS) is a well-known source of several inflammatory, cytotoxic and genotoxic compounds that cause chronic lung diseases. The transient receptor potential ankyrin 1 (TRPA1), a smoking-responsive, non-selective cation channel, is expressed by both capsaicin-sensitive peptidergic sensory nerves and non-neuronal cells of the lung, but there are few and controversial data on its expression and function on macrophages. Here, we investigated TRPA1 mRNA and protein expression in mouse and human lung tissues and human 3D spheroids, with a particular focus on its expression and potential regulatory effects on pro- and anti-inflammatory macrophage functions in response to CS. TRPA1 was stably expressed in both human and mouse alveolar macrophages, being upregulated after CS exposure and its functional activity was demonstrated in mouse macrophage culture. Moreover, besides CS, the TRPA1 genotype itself affected the expression of M1- (Il-1β, Il-23) and M2-type (Il-10, Tgfβ) macrophage cytokines. Furthermore, CS extract increased TRPA1 mRNA in human lung spheroids showing more prominent expression in macrophage-containing 3D aggregates, while CS extract influenced an elevated TGFβ expression specifically in macrophage-containing spheroids. These results suggest the fine-tuning role of TRPA1 activation in CS-induced airway inflammation, particularly in macrophages, but further studies are needed to draw precise conclusions. LA - English DB - MTMT ER - TY - JOUR AU - Török, Zsófia Laura AU - Garai, Kitti AU - Bovári-Biri, Judit AU - Ádám, Zoltán Mihály AU - Miskei, Judith Anna AU - Kajtár, Béla AU - Sárosi, Veronika AU - Pongrácz, Judit TI - Serum and exosome WNT5A levels as biomarkers in non-small cell lung cancer. JF - RESPIRATORY RESEARCH J2 - RESP RES VL - 26 PY - 2025 IS - 1 PG - 12 SN - 1465-9921 DO - 10.1186/s12931-025-03216-7 UR - https://m2.mtmt.hu/api/publication/36102755 ID - 36102755 N1 - Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, 2 Rokus Str, Pecs, H-7624, Hungary Department of Pulmonology, 1st Internal Medicine, The Medical School and Clinical Centre, University of Pecs, 12 Szigeti Str, H-7624, Hungary Department of Pathology, The Medical School and Clinical Centre, University of Pecs, 12 Szigeti Str, H-7624, Hungary Export Date: 16 July 2025; Cited By: 1; Correspondence Address: J.E. Pongracz; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, 2 Rokus Str, Pecs, H-7624, Hungary; email: pongracz.e.judit@pte.hu; CODEN: RREEB AB - Despite significant advances in the treatment of lung cancer (LC), there are no reliable biomarkers to effectively predict therapy response and overall survival (O/S) in non-small cell lung cancer (NSCLC) subtypes. While targeted therapies have improved survival rates in lung adenocarcinoma (LUAD), effective treatment options for lung squamous cell carcinoma (LUSC) are still limited. Recent evidence indicates that exosome-bound WNT5A may significantly contribute to disease progression. Our study assessed the WNT5A protein as a potential biomarker for diagnosing patients and predicting prognosis to assist in therapy selection.Primary tumor tissue and serum samples were collected from a cohort of 60 patients with histologically confirmed NSCLC before therapy. Healthy serum donors served as controls. Exosomes were isolated, then exosome number and size were measured, and WNT5A protein levels were identified in tissue and in vesicle-free, vesicle-bound fractions of the serum by ELISA.Extensive statistical analysis (ROC, AUC, Cox, etc.) revealed that elevated WNT5A levels on the serum-exosome surface correlated with distant metastasis, advanced disease stage, and lymph node involvement in LUSC but not in LUAD patients. Moreover, a high WNT5A exosome surface expression was associated with a poor response to therapy and shorter O/S in LUSC patients. Additionally, serum-exosome surface + cargo WNT5A content distinguished LUAD and LUSC subtypes.WNT5A, particularly its serum exosome-bound form, may serve as a valuable biomarker after further validation for differentiating NSCLC subtypes and predicting disease progression. Importantly, the information can become available from a simple serum sample at the time of diagnosis. LA - English DB - MTMT ER - TY - CONF AU - Kisjós, Bálint AU - Garai, Kitti AU - Kvell, Krisztián ED - Ángyán, Virág Diána TI - Anti-tumor effect of regular physical activity-related artificial microRNAs T2 - Abstract Book of the 8th International Interdisciplinary Cholnoky Symposium PB - László Cholnoky College of Advanced Studies C1 - Pécs SN - 9789636264109 PY - 2025 SP - [30] PG - 1 UR - https://m2.mtmt.hu/api/publication/36113891 ID - 36113891 LA - English DB - MTMT ER - TY - JOUR AU - Csala, Dávid AU - Ádám, Zoltán Mihály AU - Wilhelm, Márta TI - The Role of miRNAs and Extracellular Vesicles in Adaptation After Resistance Exercise: A Review JF - CURRENT ISSUES IN MOLECULAR BIOLOGY J2 - CURR ISSUES MOL BIOL VL - 47 PY - 2025 IS - 8 PG - 34 SN - 1467-3037 DO - 10.3390/cimb47080583 UR - https://m2.mtmt.hu/api/publication/36284032 ID - 36284032 N1 - Journal Article; Review AB - Resistance exercise can enhance or preserve muscle mass and/or strength. Modifying factors are secreted following resistance exercise. Biomarkers like cytokines and extracellular vesicles, especially small extracellular vesicles, are released into the circulation and play an important role in cell-to-cell and inter-tissue communications. There is increasing evidence that physical activity itself promotes the release of extracellular vesicles into the bloodstream, suggesting the importance of vesicles in mediating systemic adaptations following exercise. Extracellular vesicles contain proteins, nucleic acids like miRNAs, and other molecules targeting different cell types and tissues of distant organs. Therefore, extracellular vesicles and encapsulated miRNAs are fine tuners of protein synthesis and are important in the adaptation after resistance training. However, there is a lack of strong data supporting the precise mechanisms of these processes. In this literature review, we collected publications related to miRNA and extracellular vesicle profile changes induced by resistance exercise. To the best of our knowledge, the changes in human extracellular vesicle and microRNA profiles following resistance exercise have not been reviewed yet. We aimed to assess the shortcomings and difficulties characterizing this research area, to summarize the existing results to date, and to propose possible solutions that could help standardize the implementation of future investigations. LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Kinga AU - Told, Roland AU - Péntek, Attila AU - Sahai, Nitin AU - Bánfai, Krisztina AU - Vizi, András AU - Koltai, Arnold AU - Szabó, Péter AU - Gurdán, Zsuzsanna AU - Bovári-Biri, Judit AU - Pongrácz, Judit AU - Telek, Elek AU - Lukács, András Szilárd AU - Maróti, Péter TI - Surface disinfection change the mechanical, structural and biological properties of flexible materials used for additive manufacturing of medical devices JF - MATERIALS AND DESIGN J2 - MATER DESIGN VL - 237 PY - 2024 PG - 17 SN - 0264-1275 DO - 10.1016/j.matdes.2023.112616 UR - https://m2.mtmt.hu/api/publication/34473439 ID - 34473439 N1 - * Megosztott szerzőség LA - English DB - MTMT ER -