TY - JOUR AU - Iqbal, Nadeem AU - Ördög, Attila AU - Koprivanacz, Péter AU - Kukri, András AU - Czékus, Zalán AU - Poór, Péter TI - Salicylic acid- and ethylene-dependent effects of the ER stress-inducer tunicamycin on the photosynthetic light reactions in tomato plants JF - JOURNAL OF PLANT PHYSIOLOGY J2 - J PLANT PHYSIOL VL - 295 PY - 2024 PG - 8 SN - 0176-1617 DO - 10.1016/j.jplph.2024.154222 UR - https://m2.mtmt.hu/api/publication/34802566 ID - 34802566 LA - English DB - MTMT ER - TY - CHAP AU - Rozsnyói, Ákos AU - Balázs, Dóra Krisztina AU - Tyagi, Chetna AU - Terna, Gergő AU - Szekeres, András AU - Vágvölgyi, Csaba AU - Marik, Tamás AU - Kredics, László ED - Allaga, Henrietta ED - Balázs, Dóra Krisztina TI - Intelligens peptidek: mesterséges intelligencia felhasználása a peptaibolok kutatásában T2 - III. Természettudományok helyzete hazánkban Egyetemtől a munkaerőpiacig Workshop: Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457479 PY - 2024 SP - 32 EP - 33 PG - 2 UR - https://m2.mtmt.hu/api/publication/34756662 ID - 34756662 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Terna, Gergő AU - Tyagi, Chetna AU - Balázs, Dóra Krisztina AU - Rozsnyói, Ákos AU - Szekeres, András AU - Varga, Mónika AU - Vágvölgyi, Csaba AU - Kredics, László AU - Marik, Tamás ED - Allaga, Henrietta ED - Balázs, Dóra Krisztina TI - Water solubility of fungal peptaibols and its effect on their bioactivity T2 - III. Természettudományok helyzete hazánkban Egyetemtől a munkaerőpiacig Workshop: Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457479 PY - 2024 SP - 29 EP - 31 PG - 3 UR - https://m2.mtmt.hu/api/publication/34756649 ID - 34756649 LA - English DB - MTMT ER - TY - JOUR AU - Dán, Kinga AU - Kocsubé, Sándor AU - Tóth, Liliána AU - Farkas, Attila AU - Rákhely, Gábor AU - Galgóczi, László Norbert TI - Isolation and identification of fungal biodeteriogens from the wall of a cultural heritage church and potential applicability of antifungal proteins in protection JF - JOURNAL OF CULTURAL HERITAGE J2 - J CULT HERIT VL - 67 PY - 2024 SP - 194 EP - 202 PG - 9 SN - 1296-2074 DO - 10.1016/j.culher.2024.03.002 UR - https://m2.mtmt.hu/api/publication/34749009 ID - 34749009 LA - English DB - MTMT ER - TY - CHAP AU - Sávai, Gergő AU - Kartali, Tünde AU - Benci, Dániel Attila AU - Patai, Roland AU - Lipinszki, Zoltán AU - Vágvölgyi, Csaba AU - Papp, Tamás TI - Mikovírusok azonosítása Rhizopus fajokban T2 - Biotechnológiai Szakmai Nap Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457448 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34723493 ID - 34723493 LA - Hungarian DB - MTMT ER - TY - GEN AU - Turek, Cezary AU - Olbei, Marton AU - Stirling, Tamás AU - Fekete, Gergely AU - Tasnádi, Ervin Áron AU - Gul, Leila AU - Bohár, Balázs AU - Papp, Balázs AU - Jurkowski, Wiktor AU - Ari, Eszter TI - mulea - an R package for enrichment analysis using multiple ontologies and empirical FDR correction PY - 2024 UR - https://m2.mtmt.hu/api/publication/34718081 ID - 34718081 AB - Traditional gene set enrichment analyses are typically limited to a few ontologies and do not account for the interdependence of gene sets or terms, resulting in overcorrected p-values. To address these challenges, we introduce mulea, an R package offering comprehensive overrepresentation and functional enrichment analysis. mulea employs an innovative empirical false discovery rate (eFDR) correction method, specifically designed for interconnected biological data, to accurately identify significant terms within diverse ontologies. mulea expands beyond traditional tools by incorporating a wide range of ontologies, encompassing Gene Ontology, pathways, regulatory elements, genomic locations, and protein domains. This flexibility enables researchers to tailor enrichment analysis to their specific questions, such as identifying enriched transcriptional regulators in gene expression data or overrepresented protein domains in protein sets. To facilitate seamless analysis, mulea provides gene sets (in standardised GMT format) for 27 model organisms, covering 16 databases and various identifiers resulting in almost 900 files. Additionally, the muleaData ExperimentData Bioconductor package simplifies access to these pre-defined ontologies. Finally, mulea's architecture allows for easy integration of user-defined ontologies, expanding its applicability across diverse research areas. Availability and Implementation: Software for the tools demonstrated in this article is available as an R package on GitHub: https://github.com/ELTEbioinformatics/mulea. LA - English DB - MTMT ER - TY - JOUR AU - Vágvölgyi, Máté AU - Laczkó, Dávid AU - Santa Maria, Anaraquel AU - Vigh, Judit Piroska AU - Walter, Fruzsina AU - Berkecz, Róbert AU - Deli, Mária Anna AU - Tóth, Gábor AU - Hunyadi, Attila TI - 17-Oxime ethers of oxidized ecdysteroid derivatives modulate oxidative stress in human brain endothelial cells and dose-dependently might protect or damage the blood-brain barrier JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 2 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0290526 UR - https://m2.mtmt.hu/api/publication/34691003 ID - 34691003 N1 - Institute of Pharmacognosy, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, United States Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Pharmaceutical Analysis, University of Szeged, Szeged, Hungary NMR Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary Interdisciplinary Centre of Natural Products, University of Szeged, Szeged, Hungary HUN-REN-SZTE Biologically Active Natural Products Research Group, Szeged, Hungary Export Date: 18 March 2024 CODEN: POLNC Correspondence Address: Hunyadi, A.; Institute of Pharmacognosy, Hungary; email: hunyadi.attila@szte.hu AB - 20-Hydroxyecdysone and several of its oxidized derivatives exert cytoprotective effect in mammals including humans. Inspired by this bioactivity of ecdysteroids, in the current study it was our aim to prepare a set of sidechain-modified derivatives and to evaluate their potential to protect the blood-brain barrier (BBB) from oxidative stress. Six novel ecdysteroids, including an oxime and five oxime ethers, were obtained through regioselective synthesis from a sidechain-cleaved calonysterone derivative 2 and fully characterized by comprehensive NMR techniques revealing their complete 1 H and 13 C signal assignments. Surprisingly, several compounds sensitized hCMEC/D3 brain microvascular endothelial cells to tert -butyl hydroperoxide (tBHP)-induced oxidative damage as recorded by impedance measurements. Compound 8 , containing a benzyloxime ether moiety in its sidechain, was the only one that exerted a protective effect at a higher, 10 μM concentration, while at lower (10 nM– 1 μM) concentrations it promoted tBHP-induced cellular damage. Brain endothelial cells were protected from tBHP-induced barrier integrity decrease by treatment with 10 μM of compound 8 , which also mitigated the intracellular reactive oxygen species production elevated by tBHP. Based on our results, 17-oxime ethers of oxidized ecdysteroids modulate oxidative stress of the BBB in a way that may point towards unexpected toxicity. Further studies are needed to evaluate any possible risk connected to dietary ecdysteroid consumption and CNS pathologies in which BBB damage plays an important role. LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Györgyi AU - Bende, Gábor AU - Borics, Attila AU - Dán, Kinga AU - Rákhely, Gábor AU - Tóth, Gábor AU - Galgóczi, László Norbert TI - Rational Design of Antifungal Peptides Based on the γ-Core Motif of a Neosartorya (Aspergillus) fischeri Antifungal Protein to Improve Structural Integrity, Efficacy, and Spectrum JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 6 SP - 7206 EP - 7214 PG - 9 SN - 2470-1343 DO - 10.1021/acsomega.3c09377 UR - https://m2.mtmt.hu/api/publication/34627084 ID - 34627084 N1 - Funding Agency and Grant Number: , Nemzeti Kutat?si Fejleszt?si ?s Innov?ci?s Hivatal [TKP2021-EGA-32]; Hungarian National Research, Development, and Innovation OfficeyNKFIH [FK 134343]; Hungarian National Research Development and Innovation OfficeyNKFIH; University of Szeged Open Access Fund [6653] Funding text: G.V. and G.K.T. were supported by the TKP2021-EGA-32 fund of the Hungarian National Research, Development, and Innovation OfficeyNKFIH. The present work of L.G. was financed by the Hungarian National Research Development and Innovation OfficeyNKFIH, FK 134343 project. The open-access publishing was supported by the University of Szeged Open Access Fund; grant number: 6653. LA - English DB - MTMT ER - TY - JOUR AU - Páll-Gergely, Barna AU - Sipos, András Árpád AU - Harzhauser, Mathias AU - Örstan, Aydın AU - Winkler, Viola AU - Neubauer, Thomas A TI - Many roads to success: Alternative routes to building an economic shell in land snails JF - EVOLUTION J2 - EVOLUTION VL - 78 PY - 2024 IS - 4 SP - 778 EP - 786 PG - 9 SN - 0014-3820 DO - 10.1093/evolut/qpae018 UR - https://m2.mtmt.hu/api/publication/34542705 ID - 34542705 N1 - Funding Agency and Grant Number: Hungarian Research Fund [OTKA FK 135262, OTKA K134199]; Bolyai Research Scholarship of the Hungarian Academy of Sciences; National Research, Development, and Innovation Fund of Hungary [TKP2021-NVA-02] Funding text: This study was supported by the Hungarian Research Fund grants OTKA FK 135262 (B.P.G.) and OTKA K134199 (A.A.S.), the Bolyai Research Scholarship of the Hungarian Academy of Sciences for B.P.G., and the National Research, Development, and Innovation Fund of Hungary under grant TKP2021-NVA-02 (A.A.S.). AB - Land snails exhibit an extraordinary variety of shell shapes. The way shells are constructed underlie biological and mechanical constraints that vary across gastropod clades. Here, we quantify shell geometry of the two largest groups, Stylommatophora and Cyclophoroidea, to assess the potential causes for variation in shell shape and its relative frequency. Based on µCT scans, we estimate material efficiency through 2D- and 3D-generalisations of the isoperimetric ratio, quantifying the ratios between area and perimeter of whorl cross-sections (2D) and shell volume and surface (3D), respectively. We find that stylommatophorans optimise material usage through whorl overlap, which may have promoted the diversification of flat-shelled species. Cyclophoroids are bound to a circular cross-section because of their operculum; flat shells are comparatively rare. Both groups show similar solutions for tall shells, where local geometry has a smaller effect because of the double overlap between previous and current whorls. Our results suggest that material efficiency is a driving factor in the selection of shell geometry. Essentially, the evolutionary success of Stylommatophora likely roots in their higher flexibility to produce an economic shell. LA - English DB - MTMT ER - TY - JOUR AU - Gombás, Bence György AU - Villanyi, Zoltan TI - 1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25031611 UR - https://m2.mtmt.hu/api/publication/34540973 ID - 34540973 AB - We recently demonstrated that 1,6-hexanediol inhibits the formation of assemblysomes. These membraneless cell organelles have important roles in co-translational protein complex assembly and also store halfway translated DNA damage response proteins for a timely stress response. Recognizing the therapeutic potential of 1,6-hexanediol in dismantling assemblysomes likely to be involved in chemo- or radiotherapy resistance of tumor cells, we initiated an investigation into the properties of 1,6-hexanediol. Our particular interest was to determine if this compound induces DNA double-strand breaks by releasing the BLM helicase. Its yeast ortholog Sgs1 was confirmed to be a component of assemblysomes. The BLM helicase induces DNA damage when overexpressed due to the DNA double-strand breaks it generates during its normal function to repair DNA damage sites. It is evident that storing Sgs1 helicase in assemblysomes is crucial to express the full-length functional protein only in the event of DNA damage. Alternatively, if we dissolve assemblysomes using 1,6-hexanediol, ribosome-nascent chain complexes might become targets of ribosome quality control. We explored these possibilities and found, through the Drosophila wing-spot test assay, that 1,6-hexanediol induces DNA double-strand breaks. Lethality connected to recombination events following 1,6-hexanediol treatment can be mitigated by inducing DNA double-strand breaks with X-ray. Additionally, we confirmed that SMC5 recruits DmBLM to DNA damage sites, as knocking it down abolishes the rescue effect of DNA double-strand breaks on 1,6-hexanediol-induced lethality in Drosophila melanogaster. LA - English DB - MTMT ER -