TY - JOUR AU - Ahmed, Marwa AU - Hessz, Dóra AU - Gyarmati, Benjámin Sándor AU - Pancsics, Mirko AU - Kovács, Norbert AU - Gyurcsányi, Ervin Róbert AU - Kubinyi, Miklós AU - Horváth, Viola TI - A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy JF - NANOSCALE J2 - NANOSCALE VL - 16 PY - 2024 IS - 7 SP - 3659 EP - 3667 PG - 9 SN - 2040-3364 DO - 10.1039/d3nr02460a UR - https://m2.mtmt.hu/api/publication/34569163 ID - 34569163 N1 - Funding Agency and Grant Number: Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund under the TKP2021 funding scheme; National Research, Development and Innovation (NRDI) Office, Hungary [BME-EGA-02]; Hungarian Academy of Sciences; Stipendium Hungaricum Scholarship; [FK 138029] Funding text: The research reported in this paper is part of project no. BME-EGA-02, implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. Further support was provided by the National Research, Development and Innovation (NRDI) Office, Hungary via grant FK 138029. B. Gy. and M. A. A. acknowledge the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences and the Stipendium Hungaricum Scholarship, respectively. AB - Quantitation of protein-nanoparticle interactions is essential for the investigation of the protein corona around NPs in vivo and when using synthetic polymer nanoparticles as affinity reagents for selective protein recognition in vitro. Here, a method based on steady-state fluorescence anisotropy measurement is presented as a novel, separation-free tool for the assessment of protein-nanoparticle interactions. For this purpose, a long-lifetime luminescent Ru-complex is used for protein labelling, which exhibits low anisotropy when conjugated to the protein but displays high anisotropy when the proteins are bound to the much larger polymer nanoparticles. As a proof of concept, the interaction of lysozyme with poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-acrylic acid) nanoparticles is studied, and fluorescence anisotropy measurements are used to establish the binding kinetics, binding isotherm and a competitive binding assay. Using long-lifetime fluorophores as protein labels, protein-nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive protein assays can be set up. LA - English DB - MTMT ER - TY - JOUR AU - Orbán, István AU - Ujj, Dóra Viktória AU - Mátravölgyi, Béla AU - Holczbauer, Tamás AU - Rapi, Zsolt TI - d-Idose-Based Monoaza-15-Crown-5 Lariat Ethers: Synthesis of an Elusive d-Hexose and Application of Derived Macrocycles in Enantioselective Syntheses JF - SYMMETRY (BASEL) J2 - SYMMETRY-BASEL VL - 15 PY - 2023 IS - 9 PG - 23 SN - 2073-8994 DO - 10.3390/sym15091714 UR - https://m2.mtmt.hu/api/publication/34133474 ID - 34133474 N1 - Department of organic Chemistry and Technology, Budapest University of Technology and Economics, Budapest, 1111, Hungary Centre for Structural Research, Eötvös Loránd Research Network, Magyar Tudósok Körútja 2, Budapest, 1117, Hungary Export Date: 13 October 2023 Correspondence Address: Rapi, Z.; Department of organic Chemistry and Technology, Hungary; email: rapi.zsolt@vbk.bme.hu AB - Carbohydrate-based macrocycles can be enantioselective catalysts in certain reactions. Previously, it was proven that the carbohydrate moiety could affect the catalytic activity of the monoaza-15-crown-5 type macrocycles derived from sugars. According to our experiments so far, the most effective enantioselective catalysts were the d-glucose- and the d-galactose-based crown ethers. To obtain more information about the effect of the carbohydrate unit, a rare monosaccharide, d-idose was incorporated into the monoaza-15-crown-5 structure. The key intermediates were methyl 4,6-O-benzylidene-α-d-idopyranoside and methyl 4,6-O-benzylidene-β-d-idopyranoside, which were synthesized from d-galactose. The efficiency of the idopyranoside-based crown compounds synthesized was investigated in asymmetric phase transfer reactions. In liquid-liquid biphasic reactions the highest enantioselectivity was 81% ee, while in solid-liquid phase systems the highest asymmetric induction was 67% ee. It was observed that the enantiodiscrimination was strongly dependent on the configuration of the anomeric center, on the side arm of the nitrogen, and on the structure of the substrate. LA - English DB - MTMT ER - TY - JOUR AU - Szalai, Zsuzsanna AU - Tóth, Boldizsár AU - Szabó, Rita (Oláhné) AU - Bősze, Szilvia AU - Karaghiosoff, Konstantin AU - Czugler, Mátyás AU - Drahos, László AU - Keglevich, György TI - A Study of the Bisphosphonic Derivatives from the Pudovik Reaction of Dialkyl α-Oxophosphonates and >P(O)H Reagents: X-ray Structure and Bioactivity JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 16 PG - 15 SN - 1420-3049 DO - 10.3390/molecules28166037 UR - https://m2.mtmt.hu/api/publication/34096300 ID - 34096300 N1 - Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Budapest, 1521, Hungary Eötvös Loránd Research Network (ELKH), Research Group of Peptide Chemistry, Eötvös Loránd University, Budapest, 1117, Hungary Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4, Budapest, 1089, Hungary Department Chemie, Ludwig-Maximilians-Universitat München, Butenandtstr. 5-13, München, D-81377, Germany MS Proteomics Research Group, Research Centre for Natural Sciences, Budapest, 1117, Hungary Export Date: 7 September 2023 CODEN: MOLEF Correspondence Address: Keglevich, G.; Department of Organic Chemistry and Technology, Hungary; email: keglevich.gyorgy@vbk.bme.hu AB - New hydroxy-methylenebisphosphonic derivatives were prepared with different P-functions. The outcome of the reaction of α-oxophosphonates (YC(O)P(O)(OR)2) and dialkyl phosphites or diarylphosphine oxides depended on the Y substituent of the oxo-compound, the nature of the P-reagent and the amount of the diethylamine catalyst. Starting from dimethyl α-oxoethylphosphonate, in the presence of 5% of diethylamine, the corresponding Pudovik adduct was the single product. While using 40% of the catalyst, the rearranged species with the >P(O)–O–CH–P(O)< skeleton was the exclusive component. A similar reaction of α-oxobenzylphosphonate followed the rearrangement protocol. X-ray crystallography revealed not only the spatial structures of the three products, but also an intricate pattern evolving from the interplay of slight chemical differences, solvent inclusion and disorder as well as H-bridge patterns, which invite further investigation. In vitro activity of the compounds was assessed on different tumor cell cultures using end-point-type cell tetrazolium-based measurements. These structure–activity studies revealed a cytostatic effect for four rearranged derivatives containing aromatic units. One of them had a pronounced effect on MDA-MB 231 and Ebc-1 cells, showing IC50 = 37.8 and 25.9 µM, respectively. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Dóra AU - Hajdinák, Péter AU - Makk-Merczel, Kinga AU - Szarka, András TI - The Possible Connection of Ferroptosis and the JNK Pathway JF - FREE RADICAL BIOLOGY AND MEDICINE J2 - FREE RADICAL BIO MED VL - 201 PY - 2023 SP - 30 SN - 0891-5849 DO - 10.1016/j.freeradbiomed.2023.03.127 UR - https://m2.mtmt.hu/api/publication/33861620 ID - 33861620 N1 - Supplement: 1 LA - English DB - MTMT ER - TY - JOUR AU - Makk-Merczel, Kinga Szilvia AU - Szarka, András AU - Varga, Dóra AU - Hajdinák, Péter TI - ROS based synergetic anticancer effect of pharmacologic ascorbate and chloroquine derivatives JF - FREE RADICAL BIOLOGY AND MEDICINE J2 - FREE RADICAL BIO MED VL - 201 PY - 2023 SP - 31 EP - 32 PG - 2 SN - 0891-5849 DO - 10.1016/j.freeradbiomed.2023.03.131 UR - https://m2.mtmt.hu/api/publication/33861592 ID - 33861592 N1 - Supplement: 1 LA - English DB - MTMT ER - TY - CHAP AU - Németh, Imre AU - Szikszai, Sára AU - Molnár, Mónika ED - Koncz, István ED - Szova, Ilona TI - Cink-oxid és titán-dioxid nanorészecskék hatása a Pseudomonas aeruginosa baktérium életjelenségeire T2 - TIZENKILENC ÉVE AZ EURÓPAI MAGYARORSZÁG TUDOMÁNYOS MEGÚJULÁSA ÉS A FIATAL KUTATÓK SZOLGÁLATÁBAN : A PEME XXIII. PhD – Konferenciájának előadásai (Budapest, 2022. április 28.) PB - Professzorok az Európai Magyarországért Egyesület CY - Budapest SN - 9786155709166 PY - 2022 SP - 104 EP - 113 PG - 10 UR - https://m2.mtmt.hu/api/publication/34603561 ID - 34603561 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Pálovics, Emese Csilla AU - Bánhegyi, Dorottya Fruzsina ED - Reedijk, J. TI - The Behavior of Not Only Structurally Similar Molecules in the Resolution Processes T2 - Reference Module in Chemistry, Molecular Sciences and Chemical Engineering PB - Elsevier CY - Waltham (MA) SN - 9780124095472 PY - 2022 PG - 8 DO - 10.1016/B978-0-32-390644-9.00009-3 UR - https://m2.mtmt.hu/api/publication/33758572 ID - 33758572 LA - English DB - MTMT ER - TY - GEN AU - Jaksics, Edina AU - Farkas, Alexandra AU - Németh, Renáta AU - Schall, Eszter AU - Szentmiklóssy, Marietta AU - Zsuzsanna, Kormosné Bugyi AU - Muskovics, Gabriella AU - Tömösközi, Sándor TI - Improving the conditions of human utilization of oat and rye in terms of processing technology and nutritional value PY - 2022 UR - https://m2.mtmt.hu/api/publication/33627140 ID - 33627140 LA - English DB - MTMT ER - TY - GEN AU - Jaksics, Edina AU - Horváth, Réka AU - Drozdik, Álmos Attila AU - Csányi, Brigitta Viktória AU - Farkas, Alexandra AU - Németh, Renáta AU - Tömösközi, Sándor TI - Rozsfajták és új malomipari frakcióik összetételi és technológiai tulajdonságainak jellemzése PY - 2022 UR - https://m2.mtmt.hu/api/publication/33627085 ID - 33627085 LA - Hungarian DB - MTMT ER - TY - GEN AU - Jaksics, Edina AU - Tömösközi, Sándor TI - A búza és egyéb gabonák funkcionális tulajdonságainak jellemzése, valamint a reológiai tulajdonságok és a beltartalmi összetétel között kapcsolat feltérképezése PY - 2022 UR - https://m2.mtmt.hu/api/publication/33627077 ID - 33627077 LA - Hungarian DB - MTMT ER -