TY - JOUR AU - Máthé, Csaba AU - Bóka, Károly AU - Kónya, Zoltán AU - Erdődi, Ferenc AU - Vasas, Gábor AU - Freytag, Csongor AU - Garda, Tamás TI - Microcystin-LR, a cyanotoxin, modulates division of higher plant chloroplasts through protein phosphatase inhibition and affects cyanobacterial division JF - CHEMOSPHERE J2 - CHEMOSPHERE VL - 358 PY - 2024 PG - 11 SN - 0045-6535 DO - 10.1016/j.chemosphere.2024.142125 UR - https://m2.mtmt.hu/api/publication/34836717 ID - 34836717 LA - English DB - MTMT ER - TY - JOUR AU - Isotta, Sturniolo AU - Csongor, Váróczy AU - Ákos, Máté Bede AU - Hegedűs, Csaba AU - Demény, Máté Ágoston AU - Virág, László TI - Quantifying antibody-dependent cellular cytotoxicity in a tumor spheroid model : application for drug discovery JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2024 SN - 1940-087X UR - https://m2.mtmt.hu/api/publication/34820104 ID - 34820104 LA - English DB - MTMT ER - TY - JOUR AU - Szántó, Magdolna AU - Yélamos, J AU - Bay, Péter TI - Specific and shared biological functions of PARP2 – is PARP2 really a lil’ brother of PARP1? JF - EXPERT REVIEWS IN MOLECULAR MEDICINE J2 - EXPERT REV MOL MED VL - 26 PY - 2024 IS - e13 SP - 1 EP - 21 PG - 21 SN - 1462-3994 UR - https://m2.mtmt.hu/api/publication/34817464 ID - 34817464 LA - English DB - MTMT ER - TY - JOUR AU - Homolya, Levente AU - Mathomes, Rachel T. AU - Fodor-Varga, Luca Anna AU - Docsa, Tibor AU - Juhász, László AU - Hayes, Joseph M. AU - Somsák, László TI - Synthesis, in silico and kinetics evaluation of N-(beta-D-glucopyranosyl)-2-arylimidazole-4(5)-carboxamides and N-(beta-D-glucopyranosyl)-4(5)-arylimidazole-2-carboxamides as glycogen phosphorylase inhibitors JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 SP - 1 EP - 21 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25094591 UR - https://m2.mtmt.hu/api/publication/34813914 ID - 34813914 AB - Recently studied N-(β-D-glucopyranosyl)-3-aryl-1,2,4-triazole-5-carboxamides have proven to be low micromolar inhibitors of glycogen phosphorylase (GP), a validated target for the treatment of type 2 diabetes mellitus. Since in other settings, the bioisosteric replacement of the 1,2,4-triazole moiety with imidazole resulted in significantly more efficient GP inhibitors, in silico calculations using Glide molecular docking along with unbound state DFT calculations were performed on N-(β-Dglucopyranosyl)-arylimidazole-carboxamides, revealing their potential for strong GP inhibition. The syntheses of the target compounds involved the formation of an amide bond between per-O-acetylated β-D-glucopyranosylamine and the corresponding arylimidazole-carboxylic acids. Kinetics experiments on rabbit muscle GPb revealed low micromolar inhibitors, with the best inhibition constants (Kis) of ~3–4 µM obtained for 1- and 2-naphthyl-substituted N-(β-D-glucopyranosyl)-imidazolecarboxamides, 2b–c. The predicted protein–ligand interactions responsible for the observed potencies are discussed and will facilitate the structure-based design of other inhibitors targeting this important therapeutic target. Meanwhile, the importance of the careful consideration of ligand tautomeric states in binding calculations is highlighted, with the usefulness of DFT calculations in this regard proposed. LA - English DB - MTMT ER - TY - JOUR AU - Sturniolo, I AU - Váróczy, Cs AU - Regdon, Zsolt AU - Mázló, A AU - Muzsai, Sz AU - Bácsi, A AU - Intili, G AU - Hegedűs, Csaba AU - Boothby, MR AU - Holechek, J AU - Ferraris, D AU - Schüler, H AU - Virág, László TI - PARP14 contributes to the development of the tumor-associated macrophage phenotype JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 SP - 1 EP - 21 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25073601 UR - https://m2.mtmt.hu/api/publication/34754751 ID - 34754751 LA - English DB - MTMT ER - TY - JOUR AU - Lontay, Beáta TI - Ünnepelt a Debreceni Egyetem Orvosi Vegytani Intézete: 70 éves Dombrádi Viktor és Erdődi Ferenc JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - XLVIII PY - 2024 IS - 1 SP - 96 EP - 100 PG - 5 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/34747207 ID - 34747207 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ghani, Marvi AU - Zohar, Peleg AU - Ujlaki, Gyula AU - Tóth, Melinda AU - Amsalu, Hailemariam AU - Póliska, Szilárd AU - Tar, Krisztina TI - Stable knockdown of Drp1 improves retinoic acid-BDNF-induced neuronal differentiation through global transcriptomic changes and results in reduced phosphorylation of ERK1/2 independently of DUSP1 and 6 JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 12 PY - 2024 SP - 1 EP - 25 PG - 25 SN - 2296-634X DO - 10.3389/fcell.2024.1342741 UR - https://m2.mtmt.hu/api/publication/34738061 ID - 34738061 AB - Background: Dynamin-related protein Drp1 —a major mitochondrial fission protein— is widely distributed in the central nervous system and plays a crucial role in regulating mitochondrial dynamics, specifically mitochondrial fission and the organelle's shaping. Upregulated Drp1 function may contribute to the pathological progression of neurodegenerative diseases by dysregulating mitochondrial fission/ fusion. The study aims to investigate the effects of Drp1 on retinoic acid-BDNF-induced (RA-BDNF) neuronal differentiation and mitochondrial network reorganization in SH-SY5Y neuroblastoma cells. LA - English DB - MTMT ER - TY - JOUR AU - Thalwieser, Zsófia AU - Fonódi, Márton AU - Király, Nikolett AU - Csortos, Csilla AU - Boratkó, Anita TI - PP2A Affects Angiogenesis via Its Interaction with a Novel Phosphorylation Site of TSP1 JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 SP - 1844 SN - 1661-6596 DO - 10.3390/ijms25031844 UR - https://m2.mtmt.hu/api/publication/34573513 ID - 34573513 AB - Alterations in angiogenic properties play a pivotal role in the manifestation and onset of various pathologies, including vascular diseases and cancer. Thrombospondin-1 (TSP1) protein is one of the master regulators of angiogenesis. This study unveils a novel aspect of TSP1 regulation through reversible phosphorylation. The silencing of the B55α regulatory subunit of protein phosphatase 2A (PP2A) in endothelial cells led to a significant decrease in TSP1 expression. Direct interaction between TSP1 and PP2A-B55α was confirmed via various methods. Truncated TSP1 constructs were employed to identify the phosphorylation site and the responsible kinase, ultimately pinpointing PKC as the enzyme phosphorylating TSP1 on Ser93. The biological effects of B55α–TSP1 interaction were also analyzed. B55α silencing not only counteracted the increase in TSP1 expression during wound closure but also prolonged wound closure time. Although B55α silenced cells initiated tube-like structures earlier than control cells, their spheroid formation was disrupted, leading to disintegration. Cells transfected with phosphomimic TSP1 S93D exhibited smaller spheroids and reduced effectiveness in tube formation, revealing insights into the effects of TSP1 phosphorylation on angiogenic properties. In this paper, we introduce a new regulatory mechanism of angiogenesis by reversible phosphorylation on TSP1 S93 by PKC and PP2A B55α. LA - English DB - MTMT ER - TY - JOUR AU - Szeőcs, Dóra AU - Vida, Beáta AU - Petővári, Gábor AU - Póliska, Szilárd AU - Janka, Eszter Anna AU - Sipos, Adrienn AU - Uray (Davis), Karen L. AU - Sebestyén, Anna AU - Krasznai, Zoárd Tibor AU - Bay, Péter TI - Cell-free ascites from ovarian cancer patients induces Warburg metabolism and cell proliferation through TGFbeta-ERK signalling JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE PY - 2024 PG - 17 SN - 2509-2715 DO - 10.1007/s11357-023-01056-1 UR - https://m2.mtmt.hu/api/publication/34483936 ID - 34483936 N1 - Funding Agency and Grant Number: University of Debrecen; NKFIH [K142141, FK128387, FK146852, TKP2021-EGA-19, TKP2021-EGA-20]; Hungarian Academy of Sciences [POST-COVID2021-33, NKM2022-30]; National Research, Development and Innovation Fund of Hungary [TKP2021-EGA-19, TKP2021-EGA-20]; HUN-REN Hungarian Research Network Funding text: Open access funding provided by University of Debrecen. Our work was supported by grants from NKFIH (K142141, FK128387, FK146852, TKP2021-EGA-19, and TKP2021-EGA-20) and the Hungarian Academy of Sciences (POST-COVID2021-33, NKM2022-30). Project no. TKP2021-EGA-19 and TKP2021-EGA-20 were implemented with support provided by the National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-EGA funding scheme. AS is supported by the Bolyai fellowship of the Hungarian Academy of Sciences. This project received funding from the HUN-REN Hungarian Research Network. AB - Ascites plays a key role in supporting the metastatic potential of ovarian cancer cells. Shear stress and carry-over of cancer cells by ascites flow support carcinogenesis and metastasis formation. In addition, soluble factors may participate in the procarcinogenic effects of ascites in ovarian cancer. This study aimed to determine the biological effects of cell-free ascites on carcinogenesis in ovarian cancer cells. Cell-free ascites from ovarian cancer patients (ASC) non-selectively induced cell proliferation in multiple models of ovarian cancer and untransformed primary human dermal fibroblasts. Furthermore, ASC induced a Warburg-type rearrangement of cellular metabolism in A2780 ovarian cancer cells characterized by increases in cellular oxygen consumption and glycolytic flux; increases in glycolytic flux were dominant. ASC induced mitochondrial uncoupling and fundamentally reduced fatty acid oxidation. Ascites-elicited effects were uniform among ascites specimens. ASC-elicited transcriptomic changes in A2780 ovarian cancer cells included induction of the TGF beta-ERK/MEK pathway, which plays a key role in inducing cell proliferation and oncometabolism. ASC-induced gene expression changes, as well as the overexpression of members of the TGF beta signaling system, were associated with poor survival in ovarian cancer patients. We provided evidence that the activation of the autocrine/paracrine of TGF beta signaling system may be present in bladder urothelial carcinoma and stomach adenocarcinoma. Database analysis suggests that the TGF beta system may feed forward bladder urothelial carcinoma and stomach adenocarcinoma. Soluble components of ASC support the progression of ovarian cancer. These results suggest that reducing ascites production may play an essential role in the treatment of ovarian cancer by inhibiting the progression and reducing the severity of the disease. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Emese AU - Fagyas, Miklós AU - Nagy, Béla AU - Siket, Ivetta Mányiné AU - Szőke, Blanka AU - Mártha, Lilla AU - Mahdi, Mohamed AU - Erdősi, Gábor AU - Pólik, Zsófia AU - Kappelmayer, János AU - Papp, Zoltán AU - Borbély, Attila AU - Szabó, Tamás AU - Balla, József AU - Balla, György AU - Bácsi, Attila AU - Szekanecz, Zoltán AU - Bay, Péter AU - Tóth, Attila TI - Distinct subsets of anti-pulmonary autoantibodies correlate with disease severity and survival in severe COVID-19 patients JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 46 PY - 2024 IS - 2 SP - 1561 EP - 1574 PG - 14 SN - 2509-2715 DO - 10.1007/s11357-023-00887-2 UR - https://m2.mtmt.hu/api/publication/34148390 ID - 34148390 AB - Autoantibodies targeting the lung tissue were identified in severe COVID-19 patients in this retrospective study. Fifty-three percent of 104 patients developed anti-pulmonary antibodies, the majority of which were IgM class, suggesting that they developed upon infection with SARS-CoV-2. Anti-pulmonary antibodies correlated with worse pulmonary function and a higher risk of multiorgan failure that was further aggravated if 3 or more autoantibody clones were simultaneously present (multi-producers). Multi-producer patients were older than the patients with less or no autoantibodies. One of the identified autoantibodies (targeting a pulmonary protein of ~ 50 kDa) associated with worse clinical outcomes, including mortality. In summary, severe COVID-19 is associated with the development of lung-specific autoantibodies, which may worsen the clinical outcome. Tissue proteome-wide tests, such as the ones applied here, can be used to detect autoimmunity in the post-COVID state to identify the cause of symptoms and to reveal a new target for treatment. LA - English DB - MTMT ER -