TY - JOUR AU - Virág, Dávid AU - Schlosser, Gitta AU - Borbély, Adina AU - Gellén, Gabriella AU - Papp, Dávid AU - Kaleta, Zoltán AU - Dalmadiné Kiss, Borbála AU - Antal, István AU - Ludányi, Krisztina TI - A Mass Spectrometry Strategy for Protein Quantification Based on the Differential Alkylation of Cysteines Using Iodoacetamide and Acrylamide JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 SP - 4656 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25094656 UR - https://m2.mtmt.hu/api/publication/34821651 ID - 34821651 AB - Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins. LA - English DB - MTMT ER - TY - JOUR AU - Szederkényi, G. AU - Kocsis, Dorottya AU - Vághy, M.A. AU - Czárán, Domonkos Tamás AU - Sasvári, Péter AU - Lengyel, Miléna AU - Naszlady, M.B. AU - Kreis, F. AU - Antal, István AU - Csépányi-Kömi, Roland AU - Erdő, F. TI - Mathematical modeling of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0299501 UR - https://m2.mtmt.hu/api/publication/34813473 ID - 34813473 AB - Mathematical models of epidermal and dermal transport are essential for optimization and development of products for percutaneous delivery both for local and systemic indication and for evaluation of dermal exposure to chemicals for assessing their toxicity. These models often help directly by providing information on the rate of drug penetration through the skin and thus on the dermal or systemic concentration of drugs which is the base of their pharmacological effect. The simulations are also helpful in analyzing experimental data, reducing the number of experiments and translating the in vitro investigations to an in-vivo setting. In this study skin penetration of topically administered caffeine cream was investigated in a skin-on-a-chip microfluidic diffusion chamber at room temperature and at 32̊C. Also the transdermal penetration of caffeine in healthy and diseased conditions was compared in mouse skins from intact, psoriatic and allergic animals. In the last experimental setup dexamethasone, indomethacin, piroxicam and diclofenac were examined as a cream formulation for absorption across the dermal barrier. All the measured data were used for making mathematical simulation in a three-compartmental model. The calculated and measured results showed a good match, which findings indicate that our mathematical model might be applied for prediction of drug delivery through the skin under different circumstances and for various drugs in the novel, miniaturized diffusion chamber. © 2024 Szederkényi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, LA - English DB - MTMT ER - TY - JOUR AU - Kirschfink, M. AU - Frazer-Abel, A. AU - Bertalanné Balogh, Emese AU - Goseberg, S. AU - Weiss, N. AU - Prohászka, Zoltán TI - External quality assurance program for diagnostic complement laboratories: evaluation of the results of the past seven years JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 18 SN - 1664-3224 DO - 10.3389/fimmu.2024.1368399 UR - https://m2.mtmt.hu/api/publication/34796435 ID - 34796435 LA - English DB - MTMT ER - TY - JOUR AU - Kállai-Szabó, Nikolett AU - Farkas, Dóra AU - Lengyel, Miléna AU - Basa, Bálint AU - Fleck, C. AU - Antal, István TI - Microparticles and multi-unit systems for advanced drug delivery JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 194 PY - 2024 PG - 25 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106704 UR - https://m2.mtmt.hu/api/publication/34631071 ID - 34631071 N1 - Export Date: 28 February 2024 CODEN: EPSCE Correspondence Address: Antal, I.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: antal.istvan@pharma.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Budavári, Bálint Péter AU - Karancsi, Áron AU - Pinke, Balázs Gábor AU - Pállinger, Éva AU - Juriga-Tóth, Krisztina AU - Király, Márton AU - Szász, Adrienn Zsófia AU - Voszka, István AU - Molnár, Kolos AU - Kőhidai, László AU - Jedlovszky-Hajdú, Angéla AU - S. Nagy, Krisztina TI - Long-term shelf-life liposomes for delivery of prednisolone and budesonide JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 394 PY - 2024 PG - 13 SN - 0167-7322 DO - 10.1016/j.molliq.2023.123756 UR - https://m2.mtmt.hu/api/publication/34506340 ID - 34506340 N1 - Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Nagyvárad tér 4., Budapest, H-1089, Hungary Department of Polymer Engineering, Faculty of Mechanical Engineering, Budapest University of Technology and Economics, Műegyetem rkp. 3-9., Budapest, H-1111, Hungary Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4., Budapest, H-1089, Hungary Department of Pharmaceutics, Faculty of Pharmacy, Semmelweis University, Hőgyes Endre u. 7., Budapest, H-1092, Hungary Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Tűzoltó u. 37-47., Budapest, H-1094, Hungary ELKH–BME Research Group for Composite Science and Technology, Műegyetem rkp. 3., Budapest, H-1111, Hungary MTA-BME Lendület Sustainable Polymers Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 14 March 2024 CODEN: JMLID Correspondence Address: Jedlovszky-Hajdu, A.; Laboratory of Nanochemistry, Nagyvárad tér 4., Hungary; email: hajdu.angela@med.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Dániel AU - Gömöry, Ágnes AU - Ludányi, Krisztina AU - Vékey, Károly AU - Drahos, László TI - Very Low-Pressure CID Experiments: High Energy Transfer and Fragmentation Pattern at the Single Collision Regime JF - MOLECULES J2 - MOLECULES VL - 29 PY - 2024 IS - 1 PG - 11 SN - 1420-3049 DO - 10.3390/molecules29010211 UR - https://m2.mtmt.hu/api/publication/34457260 ID - 34457260 LA - English DB - MTMT ER - TY - JOUR AU - MAGRAMANE, Sabrina AU - Vlahovic, Kristina AU - Gordon, Péter AU - Kállai-Szabó, Nikolett AU - Zelkó, Romána AU - Antal, István AU - Farkas, Dóra TI - Inhalation Dosage Forms: A Focus on Dry Powder Inhalers and Their Advancements JF - PHARMACEUTICALS J2 - PHARMACEUTICALS-BASE VL - 16 PY - 2023 IS - 12 PG - 48 SN - 1424-8247 DO - 10.3390/ph16121658 UR - https://m2.mtmt.hu/api/publication/34405975 ID - 34405975 N1 - Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, Budapest, H-1092, Hungary Department of Electronics Technology, Budapest University of Technology and Economics, Egry J. Str. 18, Budapest, H-1111, Hungary Department of Pharmacy Administration, Semmelweis University, Hőgyes Str. 7–9, Budapest, H-1092, Hungary Export Date: 14 March 2024 Correspondence Address: Kállai-Szabó, N.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: kallai.nikolett@semmelweis.hu Correspondence Address: Farkas, D.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: farkas.dora@semmelweis.hu AB - In this review, an extensive analysis of dry powder inhalers (DPIs) is offered, focusing on their characteristics, formulation, stability, and manufacturing. The advantages of pulmonary delivery were investigated, as well as the significance of the particle size in drug deposition. The preparation of DPI formulations was also comprehensively explored, including physico-chemical characterization of powders, powder processing techniques, and formulation considerations. In addition to manufacturing procedures, testing methods were also discussed, providing insights into the development and evaluation of DPI formulations. This review also explores the design basics and critical attributes specific to DPIs, highlighting the significance of their optimization to achieve an effective inhalation therapy. Additionally, the morphology and stability of 3 DPI capsules (Spiriva, Braltus, and Onbrez) were investigated, offering valuable insights into the properties of these formulations. Altogether, these findings contribute to a deeper understanding of DPIs and their development, performance, and optimization of inhalation dosage forms. LA - English DB - MTMT ER - TY - GEN AU - Antal, István TI - Drug delivery system for the improvement of patient centricity, compliance, and adherence PY - 2023 UR - https://m2.mtmt.hu/api/publication/34128875 ID - 34128875 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő D. AU - Kállai-Szabó, Nikolett AU - Lengyel, Miléna AU - Süvegh, Károly AU - Ender, Ferenc AU - Katona, Gábor AU - Nochta-Kazsoki, Adrienn Katalin AU - Zelkó, Romána AU - Antal, István AU - Balogh, György Tibor AU - Balogh Weiser, Diána TI - Nanoformulation of lipase from Porcine pancreas by electrospinning as a novel alternative for enzyme-based per os therapies JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 389 PY - 2023 PG - 13 SN - 0167-7322 DO - 10.1016/j.molliq.2023.122819 UR - https://m2.mtmt.hu/api/publication/34096036 ID - 34096036 N1 - Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Pharmaceutics, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Eötvös Loránd University Laboratory of Nuclear Chemistry, P.O. Box 32, Budapest, H-1518, Hungary Department of Electron Devices, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Spinsplit Llc., Vend u. 17, Budapest, H-1025, Hungary Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary University Pharmacy, Department of Pharmacy Administration, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Budapest, H-1111, Hungary Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 20 February 2024 CODEN: JMLID Correspondence Address: Antal, I.; Department of Pharmaceutical Chemistry, Hőgyes E. Street 7–9, Hungary; email: antal.istvan@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Budai, Lívia AU - Budai, Marianna AU - Pápay, Zsófia Edit AU - Szalkai, Petra AU - Niczinger, Noémi AU - Kijima, S. AU - Sugibayashi, K. AU - Antal, István AU - Kállai-Szabó, Nikolett TI - Viscoelasticity of Liposomal Dispersions JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 16 PG - 14 SN - 2079-4991 DO - 10.3390/nano13162340 UR - https://m2.mtmt.hu/api/publication/34096011 ID - 34096011 N1 - Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, Budapest, 1092, Hungary Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Saitama, Sakado, 350-0295, Japan Export Date: 21 November 2023 Correspondence Address: Antal, I.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: antal.istvan@semmelweis.hu Correspondence Address: Kállai-Szabó, N.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: kallai.nikolett@semmelweis.hu LA - English DB - MTMT ER -