TY - JOUR AU - Zhang, Le AU - El-Shabrawi, Mortada AU - Baur, Louise A AU - Byrne, Christopher D AU - Targher, Giovanni AU - Kehar, Mohit AU - Porta, Gilda AU - Lee, Way Seah AU - Lefere, Sander AU - Turan, Serap AU - Alisi, Anna AU - Weiss, Ram AU - Faienza, Maria Felicia AU - Ashraf, Ambika AU - Sundaram, Shikha S AU - Srivastava, Anshu AU - De Bruyne, Ruth AU - Kang, Yunkoo AU - Bacopoulou, Flora AU - Zhou, Yong-Hai AU - Darma, Andy AU - Lupsor-Platon, Monica AU - Hamaguchi, Masahide AU - Misra, Anoop AU - Méndez-Sánchez, Nahum AU - Ng, Nicholas Beng Hui AU - Marcus, Claude AU - Staiano, Amanda E AU - Waheed, Nadia AU - Alqahtani, Saleh A AU - Giannini, Cosimo AU - Ocama, Ponsiano AU - Nguyen, Mindie H AU - Arias-Loste, Maria Teresa AU - Ahmed, Mohamed Rabea AU - Sebastiani, Giada AU - Poovorawan, Yong AU - Al Mahtab, Mamun AU - Pericàs, Juan M AU - Reverbel da Silveira, Themis AU - Hegyi, Péter AU - Azaz, Amer AU - Isa, Hasan M AU - Lertudomphonwanit, Chatmanee AU - Farrag, Mona Issa AU - Nugud, Ahmed Abd Alwahab AU - Du, Hong-Wei AU - Qi, Ke-Min AU - Mouane, Nezha AU - Cheng, Xin-Ran AU - Al Lawati, Tawfiq AU - Fagundes, Eleonora D T AU - Ghazinyan, Hasmik AU - Hadjipanayis, Adamos AU - Fan, Jian-Gao AU - Gimiga, Nicoleta AU - Kamal, Naglaa M AU - Ștefănescu, Gabriela AU - Hong, Li AU - Diaconescu, Smaranda AU - Li, Ming AU - George, Jacob AU - Zheng, Ming-Hua TI - An international multidisciplinary consensus on pediatric metabolic dysfunction-associated fatty liver disease JF - Med J2 - Med PY - 2024 SN - 2666-6340 DO - 10.1016/j.medj.2024.03.017 UR - https://m2.mtmt.hu/api/publication/34836460 ID - 34836460 AB - Non-alcoholic fatty liver disease (NAFLD) is highly prevalent in children and adolescents, particularly those with obesity. NAFLD is considered a hepatic manifestation of the metabolic syndrome due to its close associations with abdominal obesity, insulin resistance, and atherogenic dyslipidemia. Experts have proposed an alternative terminology, metabolic dysfunction-associated fatty liver disease (MAFLD), to better reflect its pathophysiology. This study aimed to develop consensus statements and recommendations for pediatric MAFLD through collaboration among international experts.A group of 65 experts from 35 countries and six continents, including pediatricians, hepatologists, and endocrinologists, participated in a consensus development process. The process encompassed various aspects of pediatric MAFLD, including epidemiology, mechanisms, screening, and management.In round 1, we received 65 surveys from 35 countries and analyzed these results, which informed us that 73.3% of respondents agreed with 20 draft statements while 23.8% agreed somewhat. The mean percentage of agreement or somewhat agreement increased to 80.85% and 15.75%, respectively, in round 2. The final statements covered a wide range of topics related to epidemiology, pathophysiology, and strategies for screening and managing pediatric MAFLD.The consensus statements and recommendations developed by an international expert panel serve to optimize clinical outcomes and improve the quality of life for children and adolescents with MAFLD. These findings emphasize the need for standardized approaches in diagnosing and treating pediatric MAFLD.This work was funded by the National Natural Science Foundation of China (82070588, 82370577), the National Key R&D Program of China (2023YFA1800801), National High Level Hospital Clinical Research Funding (2022-PUMCH-C-014), the Wuxi Taihu Talent Plan (DJTD202106), and the Medical Key Discipline Program of Wuxi Health Commission (ZDXK2021007). LA - English DB - MTMT ER - TY - JOUR AU - Hencz, Alexandra Júlia AU - Magony, Andor Dániel AU - Thomas, Chloe AU - Kovacs, Krisztina AU - Szilágyi, Tamás Gábor AU - Pál, József AU - Sík, Attila TI - Short-term hyperoxia-induced functional and morphological changes in rat hippocampus JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 18 PY - 2024 PG - 13 SN - 1662-5102 DO - 10.3389/fncel.2024.1376577 UR - https://m2.mtmt.hu/api/publication/34836454 ID - 34836454 N1 - * Megosztott szerzőség AB - Excess oxygen (O2) levels may have a stimulating effect, but in the long term, and at high concentrations of O2, it is harmful to the nervous system. The hippocampus is very sensitive to pathophysiological changes and altered O2 concentrations can interfere with hippocampus-dependent learning and memory functions. In this study, we investigated the hyperoxia-induced changes in the rat hippocampus to evaluate the short-term effect of mild and severe hyperoxia. Wistar male rats were randomly divided into control (21% O2), mild hyperoxia (30% O2), and severe hyperoxia groups (100% O2). The O2 exposure lasted for 60 min. Multi-channel silicon probes were used to study network oscillations and firing properties of hippocampal putative inhibitory and excitatory neurons. Neural damage was assessed using the Gallyas silver impregnation method. Mild hyperoxia (30% O2) led to the formation of moderate numbers of silver-impregnated "dark" neurons in the hippocampus. On the other hand, exposure to 100% O2 was associated with a significant increase in the number of "dark" neurons located mostly in the hilus. The peak frequency of the delta oscillation decreased significantly in both mild and severe hyperoxia in urethane anesthetized rats. Compared to normoxia, the firing activity of pyramidal neurons under hyperoxia increased while it was more heterogeneous in putative interneurons in the cornu ammonis area 1 (CA1) and area 3 (CA3). These results indicate that short-term hyperoxia can change the firing properties of hippocampal neurons and network oscillations and damage neurons. Therefore, the use of elevated O2 concentration inhalation in hospitals (i.e., COVID treatment and surgery) and in various non-medical scenarios (i.e., airplane emergency O2 masks, fire-fighters, and high altitude trekkers) must be used with extreme caution. LA - English DB - MTMT ER - TY - JOUR AU - Kajos, Luca Fanni AU - Molics, Bálint AU - Than, Péter AU - Gőbel, Gyula AU - Elmer, Diána AU - Pónusz-Kovács, Dalma AU - Csákvári, Tímea AU - Kovács, Bettina AU - Horváth, Lilla AU - Bódis, József AU - Boncz, Imre TI - Comparative analysis of the quality of life regarding patients who underwent hip replacement in public versus private hospitals in Hungary JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-024-60720-4 UR - https://m2.mtmt.hu/api/publication/34836452 ID - 34836452 AB - The study aimed to investigate the impact of hip replacement surgery on the quality of life and to compare the outcomes by sociodemographic and surgical data in Hungarian public and private hospitals. Patients were selected at the Department of Orthopaedics (Clinical Centre, University of Pécs) and at the Da Vinci Private Clinic in Pécs. Patients completed the SF-36 and Oxford Hip Score (OHS) questionnaires before the surgery, 6 weeks and 3 months later. We also evaluated socio-demographic data, disease and surgical conditions. The research involved 128 patients, 60 patients in public, 68 patients in private hospital. Despite the different sociodemographic characteristics and surgical outcomes of public and private healthcare patients, both groups had significantly improved the quality of life 3 months after hip replacement surgery measured by OHS and SF-36 physical health scores (p < 0.001). In the mental health score, only the patients of the private health sector showed a significant improvement (p < 0.001). The extent of improvement did not differ between the two healthcare sectors according to the OHS questionnaire (p = 0.985). While the SF-36 physical health score showed a higher improvement for public patients (p = 0.027), the mental health score showed a higher improvement for private patients (p = 0.015). LA - English DB - MTMT ER - TY - JOUR AU - Radványi, Lilla AU - Balázs, Viktória Lilla AU - Kocsis, Béla AU - Csikós, Eszter AU - Ángyán, Virág Diána AU - Szabó, Péter AU - Biró, Viktória AU - Kocsis, Marianna AU - Farkas, Ágnes TI - Antibacterial activity of Hungarian varietal honeys against respiratory pathogens as a function of storage time JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-024-60961-3 UR - https://m2.mtmt.hu/api/publication/34836449 ID - 34836449 AB - Today, antibiotic therapies that previously worked well against certain bacteria due to their natural sensitivity, are becoming less effective. Honey has been proven to inhibit the biofilm formation of some respiratory bacteria, however few data are available on how the storage time affects the antibacterial effect. The activity of black locust, goldenrod, linden and sunflower honeys from three consecutive years (2020, 2021, 2022) was analyzed in 2022 against Gram-negative (Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) bacteria using in vitro microbiological methods. After determining the physicochemical parameters of honey, broth microdilution was applied to determine the minimum inhibitory concentration of each honey type against each bacterium, and crystal violet assay was used to test their antibiofilm effect. The possible mechanism of action was explored with membrane degradation test, while structural changes were illustrated with scanning electron microscopy. Honeys stored for one or two years were darker than fresh honeys, while older honeys had significantly lower antibacterial activity. The most remarkable inhibitory effect was exerted by linden and sunflower honeys, and P. aeruginosa proved to be the most resistant bacterium. Based on our results, honey intended for medicinal purposes should be used as fresh as possible during a treatment. LA - English DB - MTMT ER - TY - JOUR AU - Bosnyák, Inez AU - Sadek, Mustafa AU - Ranatunga, Wasantha AU - Kozicz, Tamás AU - Morava-Kozicz, Éva TI - Normal transferrin glycosylation does not rule out severe ALG1 deficiency JF - JOURNAL OF INHERITED METABOLIC DISEASE REPORTS J2 - JIMDS REPORTS PY - 2024 SN - 2192-8304 DO - 10.1002/jmd2.12415 UR - https://m2.mtmt.hu/api/publication/34836109 ID - 34836109 AB - ALG1‐CDG is a rare, clinically variable metabolic disease, caused by the defect of adding the first mannose (Man) to N‐acetylglucosamine (GlcNAc 2 )‐pyrophosphate (PP)‐dolichol to the growing oligosaccharide chain, resulting in impaired N‐glycosylation of proteins. N‐glycosylation has a key role in functionality, stability, and half‐life of most proteins. Therefore, congenital defects of glycosylation typically are multisystem disorders. Here we report a 3‐year‐old patient with severe neurological, cardiovascular, respiratory, musculoskeletal and gastrointestinal symptoms. ALG1‐CDG was suggested based on exome sequencing and Western blot analysis. Despite her severe clinical manifestations and genetic diagnosis, serum transferrin glycoform analysis was normal. Western blot analysis of highly glycosylated proteins in fibroblasts revealed decreased intercellular adhesion molecule 1 (ICAM1), but normal lysosomal associated membrane protein 1 and 2 (LAMP1 and LAMP2) expression levels. Glycoproteomics in fibroblasts showed the presence of the abnormal tetrasacharide. Reviewing the literature, we found 86 reported ALG1‐CDG patients, but only one with normal transferrin analysis. Based on our results we would like to highlight the importance of multiple approaches in diagnosing ALG1‐CDG, as normal serum transferrin glycosylation or other biomarkers with normal expression levels can occur. LA - English DB - MTMT ER - TY - JOUR AU - Kazmouz, Muhamad Yahia AU - Felinger, Attila TI - The Impact of Water as an Additive on the Elution of Some Basic Organic Compounds in Supercritical Fluid Chromatography JF - MOLECULES J2 - MOLECULES VL - 29 PY - 2024 IS - 9 PG - 15 SN - 1420-3049 DO - 10.3390/molecules29092124 UR - https://m2.mtmt.hu/api/publication/34835501 ID - 34835501 AB - In this study, water was used as an additive in the methanol-modified carbon dioxide-based eluent for the elution of some basic organic compounds from a hybrid silica column via supercritical fluid chromatography (SFC). The experiments were applied to sulfonamides, propranolol, and other organic nitrogen compounds involving aromatic rings from different classes of amine, pyrimidine, and purine with different pKa values (the pKa values for the studied analytes range from 4.6 to 10.4). The results revealed different responses to the different percentages of water addition. Adding 1~2% of water to the modifier (methanol) led to a positive effect manifested by more symmetrical peak shapes and reduced retention times for most compounds. The key factor for this improvement in the properties of chromatographic peaks is due to the adsorption of water on the silanol groups of the stationary phase, consequently resembling the phenomena observed in hydrophilic interaction liquid chromatography (HILIC). Moreover, the availability of hydrogen bond acceptor and donor sites in the analyte structure is an important factor to be considered when adding water as an additive to the modifier for improving the chromatographic peaks. However, introducing water in an amount higher than 3% resulted in perturbed chromatographic signals. It was also found that water as an additive alone could not successfully elute propranolol from the hybrid silica column with an acceptable peak shape; thus, the addition of a strong base such as amine salts was also necessary. The proposed use of a particular amount of water in the mobile phase could have a positive effect compared to the same mobile phase without water, improving the chromatographic peak properties of the elution of some basic organic compounds from the hybrid silica column. LA - English DB - MTMT ER - TY - JOUR AU - Péterfi, Zoltán TI - Az utazással kapcsolatos védőoltások JF - MAGYAR TUDOMÁNY J2 - MAGYAR TUDOMÁNY VL - 158 PY - 2024 IS - 5 SP - 651 EP - 660 PG - 10 SN - 0025-0325 DO - 10.1556/2065.185.2024.5.8 UR - https://m2.mtmt.hu/api/publication/34834666 ID - 34834666 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Gantsetseg, Garmaa AU - Bunduc, Stefania AU - Kói, Tamás AU - Hegyi, Péter AU - Csupor, Dezső AU - Dariimaa, Ganbat AU - Dembrovszky, Fanni AU - Meznerics, Fanni Adél AU - Ailar, Nasirzadeh AU - Cristina, Barbagallo AU - Kökény, Gábor TI - A Systematic Review and Meta-Analysis of microRNA Profiling Studies in Chronic Kidney Diseases JF - NON-CODING RNA J2 - NON-CODING RNA VL - 10 PY - 2024 IS - 3 PG - 26 SN - 2311-553X DO - 10.3390/ncrna10030030 UR - https://m2.mtmt.hu/api/publication/34833996 ID - 34833996 LA - English DB - MTMT ER - TY - JOUR AU - Murillo, Enrique AU - Nagy, Veronika AU - Menchaca, Dania AU - Deli, József AU - Agócs, Attila TI - Changes in the Carotenoids of Zamia dressleri Leaves during Development JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 13 PY - 2024 IS - 9 PG - 11 SN - 2223-7747 DO - 10.3390/plants13091251 UR - https://m2.mtmt.hu/api/publication/34832995 ID - 34832995 AB - It has been observed that the leaves of some Zamia species undergo a kind of “reverse ripening”; that is, they change from their original brown color to green during development. We assumed that this strange color change was due to the change in carotenoid composition, so we followed the changes for several weeks. The detailed carotenoid composition and content at different stages of development of the leaves was determined with HPLC-DAD focusing on the changes in red and yellow carotenoids. The total and relative amounts of red and yellow carotenoids were determined simultaneously from one measurement from a saponified and/or unsaponified extract. At the beginning of development, the concentration of red carotenoids was higher than that of the yellow ones; it decreased drastically until 22 days and continued to decrease slowly until they completely disappeared. The concentration of yellow carotenoids decreased at the beginning as well, but after 22 days it started to increase. The amount of red carotenoids started to decrease when the leaflet stopped growing. Lutein is the main component in old leaflets, which is not a red carotenoid precursor. Red carotenoids can always be found in their esterified form in the leaves. These findings support the hypothesis that red and yellow carotenoid accumulation are independent and probably have different functions in the leaflet. The strange color change was explained based on the compartmentalization of red and yellow carotenoids and on the changing activity of the enzyme capsanthin–capsorubin synthase responsible for the synthesis of red carotenoids capsorubin and capsanthin. LA - English DB - MTMT ER - TY - JOUR AU - Rácz, Kálmán AU - Simon, Gábor AU - Kurucz, Andrea AU - Harsányi, Gergő Tamás AU - Török, Miklós AU - Herczeg, László Tamás AU - Gergely, Péter TI - Case Report: A Sudden Thyroid-Related Death of a 15-Year-Old Girl JF - DIAGNOSTICS J2 - DIAGNOSTICS VL - 14 PY - 2024 IS - 9 PG - 9 SN - 2075-4418 DO - 10.3390/diagnostics14090905 UR - https://m2.mtmt.hu/api/publication/34832790 ID - 34832790 AB - A 15-year-old young girl was found dead at home. There were no indications of any intervention or the application of force. On the previous day, she was admitted to hospital because of palpitations, fatigue, a headache, and a swollen neck. During a physical examination, a swollen thyroid gland and tachycardia were found. In the family history, her mother had thyroid disease. According to the laboratory values, she had elevated thyroid hormone levels. After administration of beta-blockers, the patient was discharged and died at home during the night. The parents denounced the hospital for medical malpractice; therefore, a Forensic Autopsy was performed. Based on the available clinical data, the autopsy, histological and toxicological results, the cause of death was stated as multiorgan failure due to disseminated intravascular coagulation (DIC) caused by the autoimmune Graves disease. The forensic assessment of the case does not reveal medical malpractice. Post-mortem diagnoses of thyroid disorders in cases of sudden death can be challenging. However, as the reported case illustrates, the diagnosis could be established after a detailed evaluation of antemortem clinical data, autopsy results, histology, and a toxicological examination. LA - English DB - MTMT ER -