TY - CONF AU - Wittner, Lucia AU - Somogyi, Fanni AU - Kovács, Beatrix AU - Harmati, Zsófia AU - Bod, Réka AU - Nagy, Dorottya AU - Horváth, Domonkos AU - B. Dobos, Attila AU - Vanduffel, Wim AU - Zsófia Tóth, Estilla AU - Tóth, Kinga AU - Nagy, Gábor AU - G. Bagó, Attila AU - Fabó, Dániel AU - Ulbert, István AU - Hillier, Dániel TI - Autofluorescence in large brains: origins, elimination method and quantitative analysis T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34540021 ID - 34540021 N1 - Poster LA - English DB - MTMT ER - TY - JOUR AU - Bod, Réka AU - Tóth, Kinga AU - ESSAM Fawzy Ahmed Aly, Nour AU - Tóth, Estilla Zsófia AU - Erőss, Loránd AU - Entz, László AU - Bagó, Attila György AU - Fabó, Dániel AU - Ulbert, István AU - Wittner, Lucia TI - Synaptic alterations and neuronal firing in human epileptic neocortical excitatory networks JF - FRONTIERS IN SYNAPTIC NEUROSCIENCE J2 - FRONT SYNAPTIC NEURO VL - 15 PY - 2023 PG - 22 SN - 1663-3563 DO - 10.3389/fnsyn.2023.1233569 UR - https://m2.mtmt.hu/api/publication/34093583 ID - 34093583 N1 - eCollection AB - Epilepsy is a prevalent neurological condition, with underlying neuronal mechanisms involving hyperexcitability and hypersynchrony. Imbalance between excitatory and inhibitory circuits, as well as histological reorganization are relatively well-documented in animal models or even in the human hippocampus, but less is known about human neocortical epileptic activity. Our knowledge about changes in the excitatory signaling is especially scarce, compared to that about the inhibitory cell population. This study investigated the firing properties of single neurons in the human neocortex in vitro , during pharmacological blockade of glutamate receptors, and additionally evaluated anatomical changes in the excitatory circuit in tissue samples from epileptic and non-epileptic patients. Both epileptic and non-epileptic tissues exhibited spontaneous population activity (SPA), NMDA receptor antagonization reduced SPA recurrence only in epileptic tissue, whereas further blockade of AMPA/kainate receptors reversibly abolished SPA emergence regardless of epilepsy. Firing rates did not significantly change in excitatory principal cells and inhibitory interneurons during pharmacological experiments. Granular layer (L4) neurons showed an increased firing rate in epileptic compared to non-epileptic tissue. The burstiness of neurons remained unchanged, except for that of inhibitory cells in epileptic recordings, which decreased during blockade of glutamate receptors. Crosscorrelograms computed from single neuron discharge revealed both mono- and polysynaptic connections, particularly involving intrinsically bursting principal cells. Histological investigations found similar densities of SMI-32-immunopositive long-range projecting pyramidal cells in both groups, and shorter excitatory synaptic active zones with a higher proportion of perforated synapses in the epileptic group. These findings provide insights into epileptic modifications from the perspective of the excitatory system and highlight discrete alterations in firing patterns and synaptic structure. Our data suggest that NMDA-dependent glutamatergic signaling, as well as the excitatory synaptic machinery are perturbed in epilepsy, which might contribute to epileptic activity in the human neocortex. LA - English DB - MTMT ER - TY - JOUR AU - Gáll, Zsolt AU - Kelemen, Krisztina AU - Tolokán, Andrea AU - Zolcseak, István AU - Sável, István AU - Bod, Réka AU - Ferencz, Elek AU - Vancea, Szende AU - Urkon, Melinda AU - Kolcsár, Melinda TI - Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy JF - BIOMEDICINES J2 - BIOMEDICINES VL - 10 PY - 2022 IS - 8 PG - 18 SN - 2227-9059 DO - 10.3390/biomedicines10081811 UR - https://m2.mtmt.hu/api/publication/33069192 ID - 33069192 AB - Cannabidiol (CBD) showed anticonvulsant action in several preclinical models and is currently approved by regulatory agencies to treat childhood epilepsy syndromes. However, CBD treatment has limited benefits, and its long-term effects on cognition are not fully understood yet. This study aimed to examine the impact of long-term CBD treatment in the pentylenetetrazole (PTZ)-kindling model of epilepsy. Adult male Wistar rats (N = 24) received PTZ (35 mg/kg intraperitoneally) every other day until two consecutive generalized seizures occurred. CBD (60 mg/kg body weight) was administered daily by the oral route until the kindled state was achieved (n = 12). To confirm that the formulation and administration techniques were not of concern, liquid chromatography–mass spectrometry was performed to test the brain penetration of the CBD formula. As a result of CBD treatment, a lower mortality rate and significantly prolonged generalized seizure latency (925.3 ± 120.0 vs. 550.1 ± 69.62 s) were observed, while the frequency and duration of generalized seizures were not influenced. The CBD-treated group showed a significant decrease in vertical exploration in the open field test and a significant decrease in the discrimination index in the novel object recognition (NOR) test (−0.01 ± 0.17 vs. 0.57 ± 0.15, p = 0.04). The observed behavioral characteristics may be connected to the decreased thickness of the stratum pyramidale or the decreased astrogliosis observed in the hippocampus. In conclusion, CBD treatment did not prevent kindling, nor did it affect seizure frequency or duration. However, it did increase the latency to the first seizure and decreased the prolonged status epilepticus-related mortality in PTZ-kindled rats. The cognitive impairment observed in the NOR test may be related to the high dose used in this study, which may warrant further investigation. LA - English DB - MTMT ER - TY - JOUR AU - Bod, Réka AU - Rokai, János AU - Meszéna, Domokos AU - Fiáth, Richárd AU - Ulbert, István AU - Márton, Gergely TI - From End to End: Gaining, Sorting, and Employing High-Density Neural Single Unit Recordings JF - FRONTIERS IN NEUROINFORMATICS J2 - FRONT NEUROINFORM VL - 16 PY - 2022 PG - 23 SN - 1662-5196 DO - 10.3389/fninf.2022.851024 UR - https://m2.mtmt.hu/api/publication/32875539 ID - 32875539 N1 - Laboratory of Experimental Neurophysiology, Department of Physiology, Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Târgu Mureş, Târgu Mureş, Romania Integrative Neuroscience Group, Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Budapest, Hungary School of PhD Studies, Semmelweis University, Budapest, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary Cited By :5 Export Date: 6 May 2024 Correspondence Address: Márton, G.; Integrative Neuroscience Group, Hungary; email: marton.gergely@ttk.hu LA - English DB - MTMT ER - TY - JOUR AU - Mihály, István AU - Molnár, Tímea AU - Berki, Ádám József AU - Bod, Réka AU - Orbán-Kis, Károly AU - Gáll, Zsolt AU - Szilágyi, Tibor TI - Short-Term Amygdala Low-Frequency Stimulation Does not Influence Hippocampal Interneuron Changes Observed in the Pilocarpine Model of Epilepsy JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 3 PG - 16 SN - 2073-4409 DO - 10.3390/cells10030520 UR - https://m2.mtmt.hu/api/publication/31906403 ID - 31906403 N1 - Department of Physiology, Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Târgu Mureș, 540142, Romania Department of Pharmacology and Clinical Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Târgu Mureș, 540142, Romania Export Date: 18 October 2021 Correspondence Address: Mihály, I.; Department of Physiology, Romania; email: istvan.mihaly@umfst.ro LA - English DB - MTMT ER - TY - JOUR AU - István, Mihály AU - Bod, Réka AU - Károly, Orbán-Kis AU - Berki, Ádám József AU - Tibor, Szilágyi TI - Mélyagyi stimuláció hatása a magas frekvenciájúoszcillációkra krónikus epilepsziamodellben JF - BULLETIN OF MEDICAL SCIENCES / ORVOSTUDOMÁNYI ÉRTESÍTŐ J2 - ORVOSTUDOMÁNYI ÉRTESÍTŐ VL - 93 PY - 2020 IS - 2 SP - 63 EP - 70 PG - 8 SN - 1453-0953 DO - 10.2478/orvtudert-2020-0014 UR - https://m2.mtmt.hu/api/publication/33154436 ID - 33154436 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Mihály, István AU - Orbán-Kis, Károly AU - Gáll, Zsolt AU - Berki, Ádám József AU - Bod, Réka AU - Szilágyi, Tibor TI - Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy JF - BRAIN SCIENCES J2 - BRAIN SCI VL - 10 PY - 2020 IS - 11 PG - 18 SN - 2076-3425 DO - 10.3390/brainsci10110856 UR - https://m2.mtmt.hu/api/publication/31906404 ID - 31906404 LA - English DB - MTMT ER -