@CONFERENCE{MTMT:34540021, title = {Autofluorescence in large brains: origins, elimination method and quantitative analysis}, url = {https://m2.mtmt.hu/api/publication/34540021}, author = {Wittner, Lucia and Somogyi, Fanni and Kovács, Beatrix and Harmati, Zsófia and Bod, Réka and Nagy, Dorottya and Horváth, Domonkos and B. Dobos, Attila and Vanduffel, Wim and Zsófia Tóth, Estilla and Tóth, Kinga and Nagy, Gábor and G. Bagó, Attila and Fabó, Dániel and Ulbert, István and Hillier, Dániel}, booktitle = {International Neuroscience Conference, Pécs 2024}, unique-id = {34540021}, year = {2024}, orcid-numbers = {Bod, Réka/0000-0001-9744-9282} } @article{MTMT:34093583, title = {Synaptic alterations and neuronal firing in human epileptic neocortical excitatory networks}, url = {https://m2.mtmt.hu/api/publication/34093583}, author = {Bod, Réka and Tóth, Kinga and ESSAM Fawzy Ahmed Aly, Nour and Tóth, Estilla Zsófia and Erőss, Loránd and Entz, László and Bagó, Attila György and Fabó, Dániel and Ulbert, István and Wittner, Lucia}, doi = {10.3389/fnsyn.2023.1233569}, journal-iso = {FRONT SYNAPTIC NEURO}, journal = {FRONTIERS IN SYNAPTIC NEUROSCIENCE}, volume = {15}, unique-id = {34093583}, issn = {1663-3563}, abstract = {Epilepsy is a prevalent neurological condition, with underlying neuronal mechanisms involving hyperexcitability and hypersynchrony. Imbalance between excitatory and inhibitory circuits, as well as histological reorganization are relatively well-documented in animal models or even in the human hippocampus, but less is known about human neocortical epileptic activity. Our knowledge about changes in the excitatory signaling is especially scarce, compared to that about the inhibitory cell population. This study investigated the firing properties of single neurons in the human neocortex in vitro , during pharmacological blockade of glutamate receptors, and additionally evaluated anatomical changes in the excitatory circuit in tissue samples from epileptic and non-epileptic patients. Both epileptic and non-epileptic tissues exhibited spontaneous population activity (SPA), NMDA receptor antagonization reduced SPA recurrence only in epileptic tissue, whereas further blockade of AMPA/kainate receptors reversibly abolished SPA emergence regardless of epilepsy. Firing rates did not significantly change in excitatory principal cells and inhibitory interneurons during pharmacological experiments. Granular layer (L4) neurons showed an increased firing rate in epileptic compared to non-epileptic tissue. The burstiness of neurons remained unchanged, except for that of inhibitory cells in epileptic recordings, which decreased during blockade of glutamate receptors. Crosscorrelograms computed from single neuron discharge revealed both mono- and polysynaptic connections, particularly involving intrinsically bursting principal cells. Histological investigations found similar densities of SMI-32-immunopositive long-range projecting pyramidal cells in both groups, and shorter excitatory synaptic active zones with a higher proportion of perforated synapses in the epileptic group. These findings provide insights into epileptic modifications from the perspective of the excitatory system and highlight discrete alterations in firing patterns and synaptic structure. Our data suggest that NMDA-dependent glutamatergic signaling, as well as the excitatory synaptic machinery are perturbed in epilepsy, which might contribute to epileptic activity in the human neocortex.}, year = {2023}, eissn = {1663-3563}, orcid-numbers = {Bod, Réka/0000-0001-9744-9282; Tóth, Kinga/0000-0002-8751-8499; Erőss, Loránd/0000-0002-5796-5546; Fabó, Dániel/0000-0001-5141-5351; Ulbert, István/0000-0001-9941-9159; Wittner, Lucia/0000-0001-6800-0953} } @article{MTMT:33069192, title = {Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy}, url = {https://m2.mtmt.hu/api/publication/33069192}, author = {Gáll, Zsolt and Kelemen, Krisztina and Tolokán, Andrea and Zolcseak, István and Sável, István and Bod, Réka and Ferencz, Elek and Vancea, Szende and Urkon, Melinda and Kolcsár, Melinda}, doi = {10.3390/biomedicines10081811}, journal-iso = {BIOMEDICINES}, journal = {BIOMEDICINES}, volume = {10}, unique-id = {33069192}, abstract = {Cannabidiol (CBD) showed anticonvulsant action in several preclinical models and is currently approved by regulatory agencies to treat childhood epilepsy syndromes. However, CBD treatment has limited benefits, and its long-term effects on cognition are not fully understood yet. This study aimed to examine the impact of long-term CBD treatment in the pentylenetetrazole (PTZ)-kindling model of epilepsy. Adult male Wistar rats (N = 24) received PTZ (35 mg/kg intraperitoneally) every other day until two consecutive generalized seizures occurred. CBD (60 mg/kg body weight) was administered daily by the oral route until the kindled state was achieved (n = 12). To confirm that the formulation and administration techniques were not of concern, liquid chromatography–mass spectrometry was performed to test the brain penetration of the CBD formula. As a result of CBD treatment, a lower mortality rate and significantly prolonged generalized seizure latency (925.3 ± 120.0 vs. 550.1 ± 69.62 s) were observed, while the frequency and duration of generalized seizures were not influenced. The CBD-treated group showed a significant decrease in vertical exploration in the open field test and a significant decrease in the discrimination index in the novel object recognition (NOR) test (−0.01 ± 0.17 vs. 0.57 ± 0.15, p = 0.04). The observed behavioral characteristics may be connected to the decreased thickness of the stratum pyramidale or the decreased astrogliosis observed in the hippocampus. In conclusion, CBD treatment did not prevent kindling, nor did it affect seizure frequency or duration. However, it did increase the latency to the first seizure and decreased the prolonged status epilepticus-related mortality in PTZ-kindled rats. The cognitive impairment observed in the NOR test may be related to the high dose used in this study, which may warrant further investigation.}, year = {2022}, eissn = {2227-9059}, orcid-numbers = {Gáll, Zsolt/0000-0002-9091-277X; Kelemen, Krisztina/0000-0002-6113-3419; Bod, Réka/0000-0001-9744-9282; Urkon, Melinda/0000-0002-8197-3992; Kolcsár, Melinda/0000-0003-0628-035X} } @article{MTMT:32875539, title = {From End to End: Gaining, Sorting, and Employing High-Density Neural Single Unit Recordings}, url = {https://m2.mtmt.hu/api/publication/32875539}, author = {Bod, Réka and Rokai, János and Meszéna, Domokos and Fiáth, Richárd and Ulbert, István and Márton, Gergely}, doi = {10.3389/fninf.2022.851024}, journal-iso = {FRONT NEUROINFORM}, journal = {FRONTIERS IN NEUROINFORMATICS}, volume = {16}, unique-id = {32875539}, year = {2022}, eissn = {1662-5196}, orcid-numbers = {Bod, Réka/0000-0001-9744-9282; Rokai, János/0000-0001-8606-298X; Meszéna, Domokos/0000-0003-4042-2542; Fiáth, Richárd/0000-0001-8732-2691; Ulbert, István/0000-0001-9941-9159} } @article{MTMT:31906403, title = {Short-Term Amygdala Low-Frequency Stimulation Does not Influence Hippocampal Interneuron Changes Observed in the Pilocarpine Model of Epilepsy}, url = {https://m2.mtmt.hu/api/publication/31906403}, author = {Mihály, István and Molnár, Tímea and Berki, Ádám József and Bod, Réka and Orbán-Kis, Károly and Gáll, Zsolt and Szilágyi, Tibor}, doi = {10.3390/cells10030520}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {10}, unique-id = {31906403}, year = {2021}, eissn = {2073-4409}, orcid-numbers = {Mihály, István/0000-0002-4042-7901; Molnár, Tímea/0000-0003-0273-6305; Berki, Ádám József/0000-0001-7099-167X; Bod, Réka/0000-0001-9744-9282; Orbán-Kis, Károly/0000-0002-7786-0899; Gáll, Zsolt/0000-0002-9091-277X; Szilágyi, Tibor/0000-0001-6261-1163} } @article{MTMT:33154436, title = {Mélyagyi stimuláció hatása a magas frekvenciájúoszcillációkra krónikus epilepsziamodellben}, url = {https://m2.mtmt.hu/api/publication/33154436}, author = {István, Mihály and Bod, Réka and Károly, Orbán-Kis and Berki, Ádám József and Tibor, Szilágyi}, doi = {10.2478/orvtudert-2020-0014}, journal-iso = {ORVOSTUDOMÁNYI ÉRTESÍTŐ}, journal = {BULLETIN OF MEDICAL SCIENCES / ORVOSTUDOMÁNYI ÉRTESÍTŐ}, volume = {93}, unique-id = {33154436}, issn = {1453-0953}, year = {2020}, eissn = {2537-5059}, pages = {63-70}, orcid-numbers = {Bod, Réka/0000-0001-9744-9282; Berki, Ádám József/0000-0001-7099-167X} } @article{MTMT:31906404, title = {Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy}, url = {https://m2.mtmt.hu/api/publication/31906404}, author = {Mihály, István and Orbán-Kis, Károly and Gáll, Zsolt and Berki, Ádám József and Bod, Réka and Szilágyi, Tibor}, doi = {10.3390/brainsci10110856}, journal-iso = {BRAIN SCI}, journal = {BRAIN SCIENCES}, volume = {10}, unique-id = {31906404}, year = {2020}, eissn = {2076-3425}, orcid-numbers = {Mihály, István/0000-0002-4042-7901; Orbán-Kis, Károly/0000-0002-7786-0899; Gáll, Zsolt/0000-0002-9091-277X; Berki, Ádám József/0000-0001-7099-167X; Bod, Réka/0000-0001-9744-9282; Szilágyi, Tibor/0000-0001-6261-1163} }