@article{MTMT:36366345, title = {Organic Acid Production by Basfia succiniciproducens from Agro-Industrial By-Products.}, url = {https://m2.mtmt.hu/api/publication/36366345}, author = {Balázs, Márta and Péter, Izabella and Bartos, Hunor and Bodor, Zsolt and Antal, Emőke and Albert, Csilla and Miklóssy, Ildikó}, doi = {10.3390/biotech14030068}, journal-iso = {BIOTECH}, journal = {BIOTECH}, volume = {14}, unique-id = {36366345}, abstract = {A continuous effort is needed to develop sustainable production methods for industrial platform chemicals. B. succiniciproducens, a natural succinic acid-producer, can metabolize five and six carbon atoms containing sugars in pure form as well as from agro-industrial wastes. In our work, we investigate the conversion of industrial by-products, apple pomace from apple juice production, and whey waste from milk processing to succinic acid and other organic acids (lactic, formic, and acetic acid). We obtained a succinic acid yield of 0.224 g/g total consumed fermentable sugars, lactic acid yield was 0.087 g/g, in turn, formic acid was produced at a 0.034 g/g yield, and acetic acid was obtained at 0.010 g/g total consumed fermentable sugars, using a thermal pretreated apple pomace-based medium. In the case of pretreated whey-based medium formulation, we obtained a succinic acid yield of 0.236 g/g consumed lactose, while formic acid and acetic acid were produced as well (0.09 g/g and 0.101 g/g, respectively). We demonstrate that lactose is a promising carbon source for organic acid production by B. succiniciproducens, while our study is the first to propose the use of a similarly available agro-industrial by-product, apple pomace, for the fermentative production of succinic acid by B. succiniciproducens.}, keywords = {FERMENTATION; CHEESE WHEY; SUCCINATE; apple pomace; organic acid production; Basfia succiniciproducens}, year = {2025}, eissn = {2673-6284}, orcid-numbers = {Bodor, Zsolt/0000-0003-1386-6957; Albert, Csilla/0000-0001-6882-6691} } @article{MTMT:30765679, title = {METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS}, url = {https://m2.mtmt.hu/api/publication/30765679}, author = {Sinkler, Réka and Balázs, Márta and Antal, Emőke and Bartos, Hunor and Lányi, Szabolcs and Miklóssy, Ildikó}, doi = {10.24193/subbchem.2019.2.13}, journal-iso = {STUD UNIV BABES-BOLYAI CHEM}, journal = {STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA}, volume = {64}, unique-id = {30765679}, issn = {1224-7154}, abstract = {1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enzymes in a heterologous pathway leading to BDO production, which were introduced into a genome-engineered E. coli MG1655(DE3) Delta ldhA, Delta pflB strain. Knowing that the expression of recombinant proteins and gene deletions can significantly influence cellular viability, the present study was carried out to investigate the impact of the two key enzyme expression on deletion strains, helping us to analyze the physiological changes of E. coli strains and providing directions for further optimizations in order to achieve satisfying target product yields.}, keywords = {Escherichia coli; metabolic engineering; 1,4-BUTANEDIOL; heterologous enzyme}, year = {2019}, eissn = {2065-9520}, pages = {159-174} } @CONFERENCE{MTMT:31365300, title = {Analysis of Basfia Succiniciproducens Gene Expression Profile by RT-qPCR Method}, url = {https://m2.mtmt.hu/api/publication/31365300}, author = {Balázs, Márta and Antal, Emőke and Salamon, Pál Attila and Miklóssy, Ildikó and Bodor, Zsolt}, booktitle = {25th International Conference on Chemistry = XXV. Nemzetközi Vegyészkonferencia}, unique-id = {31365300}, year = {2019}, pages = {31384216}, orcid-numbers = {Bodor, Zsolt/0000-0003-1386-6957} } @{MTMT:31371984, title = {In silico examination of a malonyl-CoA-reductase enzyme for metabolic engineering purposes}, url = {https://m2.mtmt.hu/api/publication/31371984}, author = {Antal, Emőke and Salamon, Pál Attila and Miklóssy, Ildikó}, booktitle = {ABSTRACTS BOOK, 19th International Symposium and Summer School on Bioanalysis, 2019}, unique-id = {31371984}, year = {2019}, pages = {31365350} } @{MTMT:31372114, title = {Anyagcseremérnökségben alkalmazható malonil-KoA-reduktáz expressziója és jellemzése}, url = {https://m2.mtmt.hu/api/publication/31372114}, author = {Antal, Emőke and Miklóssy, Ildikó}, booktitle = {XXXIV. Országos Tudományos Diákköri Konferencia: Biológia Szekció}, unique-id = {31372114}, year = {2019}, pages = {87} } @CONFERENCE{MTMT:31386219, title = {In silico characterization of a malonyl-CoA-reductase enzyme}, url = {https://m2.mtmt.hu/api/publication/31386219}, author = {Antal, Emőke and Balázs, Márta and Bodor, Zsolt and Miklóssy, Ildikó}, booktitle = {25th International Conference on Chemistry = XXV. Nemzetközi Vegyészkonferencia}, unique-id = {31386219}, year = {2019}, pages = {31372079}, orcid-numbers = {Bodor, Zsolt/0000-0003-1386-6957} } @CONFERENCE{MTMT:31372092, title = {Studies of dehydrogenases applicable in metabolic engineering}, url = {https://m2.mtmt.hu/api/publication/31372092}, author = {Antal, Emőke and Balázs, Márta and Sinkler, Réka and Bodor, Zsolt and Miklóssy, Ildikó}, booktitle = {XXIV. Nemzetközi Vegyészkonferencia}, unique-id = {31372092}, year = {2018}, pages = {30373698}, orcid-numbers = {Bodor, Zsolt/0000-0003-1386-6957} } @mastersthesis{MTMT:31372160, title = {CRISPR/Cas9 génszerkesztési technológia}, url = {https://m2.mtmt.hu/api/publication/31372160}, author = {Antal, Emőke}, unique-id = {31372160}, year = {2018} } @mastersthesis{MTMT:31372343, title = {Anyagcseremérnökségben alkalmazható malonil-KoA-reduktáz expressziója és jellemzése}, url = {https://m2.mtmt.hu/api/publication/31372343}, author = {Antal, Emőke}, unique-id = {31372343}, year = {2018} } @CONFERENCE{MTMT:31386269, title = {A Basfia succiniciproducens bioszintetikus potenciáljának tanulmányozása}, url = {https://m2.mtmt.hu/api/publication/31386269}, author = {Balázs, Márta and Bartos, Hunor and Antal, Emőke and Miklóssy, Ildikó and Bodor, Zsolt}, booktitle = {XXIV. Nemzetközi Vegyészkonferencia}, unique-id = {31386269}, year = {2018}, pages = {3284931}, orcid-numbers = {Bodor, Zsolt/0000-0003-1386-6957} }