@article{MTMT:35697929, title = {Stimulation of Piezo1 Mechanosensitive Channels Inhibits Adipogenesis in Thyroid Eye Disease}, url = {https://m2.mtmt.hu/api/publication/35697929}, author = {Galgóczi, Erika and Orsós, István and Molnar, Zsanett and Ujhelyi, Bernadett and Steiber, Zita and Szabó, László and Dienes, Beatrix and Csernoch, László and Nagy, Endre and Katkó, Mónika}, doi = {10.1210/clinem/dgae899}, journal-iso = {J CLIN ENDOCR METAB}, journal = {JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM}, unique-id = {35697929}, issn = {0021-972X}, abstract = {Context: Increased orbital tissue volume due to matrix expansion, orbital fibroblast (OF) proliferation, and adipocyte differentiation are the hallmarks of thyroid eye disease (TED). Their combination with the presence of hyaluronan-bound excess water in the constrains of the bony orbit results in increased intraorbital pressure. High intraorbital pressure, along with changes in the mechanical properties of orbital tissues, may lead to the activation of mechanosensitive receptors. The expression and role of the Piezo1 mechanoreceptor has not been investigated in TED. Objective: We aimed to verify the expression of Piezo1 in OFs, and to study the effect of in vitro Piezo1 activation by its synthetic agonist Yoda1 on adipocyte differentiation. Methods: OF cultures established using orbital connective tissues from patients with TED and controls were studied in the presence or absence of adipogenic stimuli. Piezo1 expression was confirmed by Western Blot and immunofluorescent imaging, and its function was verified by intracellular Ca2+ measurement. Adipogenic differentiation was characterized using Oil Red O staining for lipid accumulation, real-time polymerase chain reaction for gene and Western blot for protein expressions indicative in adipogenesis. Results: OFs express functional Piezo1 channels. Differentiation into adipocytes is inherent to TED OFs. Piezo1 activation by Yoda1 inhibits the expressions of early (CEBPβ, CEBPδ) and main (PPARγ, CEBPα) transcription factors, and the terminal marker FABP4 during adipogenesis, resulting in markedly lower intracytoplasmic lipid accumulation. Conclusion: Piezo1 channels are expressed and functional in OFs. Modeling orbital pressure by in vitro Piezo1 activation reduces de novo adipogenesis of OFs derived from TED orbits.}, keywords = {Adipogenesis; PIEZO1; Yoda1; Peroxisome proliferator-activated receptor gamma (PPARγ); Graves orbitopathy; Orbital fibroblasts}, year = {2025}, eissn = {1945-7197}, orcid-numbers = {Galgóczi, Erika/0000-0002-1701-4623; Nagy, Endre/0000-0002-9286-6471} } @article{MTMT:34866147, title = {Cyclosporin A inhibits PDGF-BB induced hyaluronan synthesis in orbital fibroblasts}, url = {https://m2.mtmt.hu/api/publication/34866147}, author = {Galgóczi, Erika and Molnar, Zsanett and Katkó, Mónika and Ujhelyi, Bernadett and Steiber, Zita and Nagy, Endre}, doi = {10.1016/j.cbi.2024.111045}, journal-iso = {CHEM-BIOL INTERACT}, journal = {CHEMICO-BIOLOGICAL INTERACTIONS}, volume = {396}, unique-id = {34866147}, issn = {0009-2797}, year = {2024}, eissn = {1872-7786}, pages = {111045} } @article{MTMT:35195783, title = {Modelling of mechanical stimuli through piezo1 receptor activation and its effect on adipogenic differentiation of orbital fibroblasts}, url = {https://m2.mtmt.hu/api/publication/35195783}, author = {Galgóczi, Erika and Orsós, István and Molnar, Zsanett and Ujhelyi, Bernadett and Steiber, Zita and Szabó, László and Dienes, Beatrix and Csernoch, László and Nagy, Endre and Katkó, Mónika}, doi = {10.1530/endoabs.101.PS1-01-01}, journal-iso = {ENDOCR ABSTR}, journal = {ENDOCRINE ABSTRACTS}, volume = {101}, unique-id = {35195783}, issn = {1470-3947}, year = {2024}, eissn = {1479-6848} } @article{MTMT:35486686, title = {Characteristics of hyaluronan metabolism during myofibroblast differentiation in orbital fibroblasts}, url = {https://m2.mtmt.hu/api/publication/35486686}, author = {Papp, Fruzsina Réka and Csiki, Róbert and Katkó, Mónika and Galgóczi, Erika and Steiber, Zita and Ujhelyi, Bernadett and Nagy, Endre}, doi = {10.1530/endoabs.101.OP-07-03}, journal-iso = {ENDOCR ABSTR}, journal = {ENDOCRINE ABSTRACTS}, volume = {101}, unique-id = {35486686}, issn = {1470-3947}, year = {2024}, eissn = {1479-6848} } @article{MTMT:35486694, title = {Production of chemerin by orbital fibroblasts during myofibroblast and adipocyte differentiation}, url = {https://m2.mtmt.hu/api/publication/35486694}, author = {Csiki, Róbert and Papp, Fruzsina Réka and Galgóczi, Erika and Steiber, Zita and Ujhelyi, Bernadett and Nagy, Endre and Katkó, Mónika}, doi = {10.1530/endoabs.101.PS1-01-04}, journal-iso = {ENDOCR ABSTR}, journal = {ENDOCRINE ABSTRACTS}, volume = {101}, unique-id = {35486694}, issn = {1470-3947}, year = {2024}, eissn = {1479-6848} } @article{MTMT:35620179, title = {Characteristics of Hyaluronan Metabolism During Myofibroblast Differentiation in Orbital Fibroblasts}, url = {https://m2.mtmt.hu/api/publication/35620179}, author = {Papp, Fruzsina Réka and Katkó, Mónika and Csiki, Róbert and Galgóczi, Erika and Molnar, Zsanett and Erdei, Annamária and Bodor, Miklós and Steiber, Zita and Ujhelyi, Bernadett and Nagy, Endre}, doi = {10.1167/iovs.65.13.13}, journal-iso = {INVEST OPHTH VIS SCI}, journal = {INVESTIGATIVE OPHTHALMOLOGY AND VISUAL SCIENCE}, volume = {65}, unique-id = {35620179}, issn = {0146-0404}, abstract = {PURPOSE. To study the impact of myofibroblast differentiation (MD) on hyaluronan (HA) turnover in orbital fibroblasts (OFs) focusing on the expression of its key enzymes and their potential implications in the pathogenesis of thyroid eye disease (TED). METHODS. Primary cultures of OFs were established from tissue samples (TED OFs, n = 4; non-TED OFs, n = 5). MD was induced by TGF-β1 (5 ng/mL). Measurements were performed after 24- and 72-hour treatments. The proliferation rate was determined by 5-bromo-2 -deoxyuridine (BrdU) incorporation. HA level and size were measured using an aggrecan-based ELISA-like method and agarose gel electrophoresis, respectively. mRNA expressions of myofibroblast markers and enzymes with a role in HA metabolism were determined using real-time PCR. RESULTS. Upregulation of type I collagen alpha1 chain, alpha-smooth muscle actin, and fibronectin indicated that OFs underwent MD after stimulation by TGF-β. After 72 hours, proliferation of untreated cultures declined, but it remained higher in myofibroblasts. Pericellular HA content, but not HA in the supernatant of myofibroblasts, increased compared to untreated cells. TGF-β was a potent stimulator of hyaluronan synthase 1 (HAS1) expression. The expression of hyaluronidase-1 and cell migration–inducing protein (CEMIP) diminished following MD, whereas the expression of transmembrane protein 2, the regulator of HA catabolism through CEMIP, was elevated. The size distribution of HA shifted toward a high-molecular-weight form following treatment with TGF-β. CONCLUSIONS. OFs undergoing MD are characterized by decreased HA turnover as a consequence of the inhibition of hyaluronidases and HAS1 induction. Our results suggest that hyaluronidases could be potential targets in the treatment of TED.}, year = {2024}, eissn = {1552-5783} } @article{MTMT:33578444, title = {Glucocorticoids Directly Affect Hyaluronan Production of Orbital Fibroblasts; A Potential Pleiotropic Effect in Graves’ Orbitopathy}, url = {https://m2.mtmt.hu/api/publication/33578444}, author = {Galgóczi, Erika and Katkó, Mónika and Papp, Fruzsina Réka and Csiki, Róbert and Bak-Csiha, Sára and Erdei, Annamária and Bodor, Miklós and Ujhelyi, Bernadett and Steiber, Zita and Győry, Ferenc and Nagy, Endre}, doi = {10.3390/molecules28010015}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {28}, unique-id = {33578444}, issn = {1431-5157}, abstract = {Orbital connective tissue expansion is a hallmark of Graves’ orbitopathy (GO). In moderate-to-severe active GO, glucocorticoids (GC) are the first line of treatment. Here we show that hydrocortisone (HC), prednisolone (P), methylprednisolone (MP), and dexamethasone (DEX) inhibit the hyaluronan (HA) production of orbital (OF) and dermal (DF) fibroblasts. HA production of GO OFs (n = 4), NON-GO OFs (n = 4) and DFs (n = 4) was measured by ELISA. mRNA expression of enzymes of HA metabolism and fibroblast proliferation was examined by RT-PCR and BrdU incorporation, respectively. After 24 h of GC treatment (1µM) HA production decreased by an average of 67.9 ± 3.11% (p < 0.0001) in all cell cultures. HAS2, HAS3 and HYAL1 expression in OFs also decreased (p = 0.009, p = 0.0005 and p = 0.015, respectively). Ten ng/mL PDGF-BB increased HA production and fibroblast proliferation in all cell lines (p < 0.0001); GC treatment remained effective and reduced HA production under PDGF-BB-stimulated conditions (p < 0.0001). MP and DEX reduced (p < 0.001, p = 0.002, respectively) PDGF-BB-induced HAS2 expression in OFs. MP and DEX treatment decreased PDGF-BB stimulated HAS3 expression (p = 0.035 and p = 0.029, respectively). None of the GCs tested reduced the PDGF-BB stimulated proliferation rate. Our results confirm that GCs directly reduce the HA production of OFs, which may contribute to the beneficial effect of GCs in GO.}, year = {2023}, eissn = {1420-3049} } @book{MTMT:34323209, title = {Ophthalmogy Practice Notes}, url = {https://m2.mtmt.hu/api/publication/34323209}, author = {Fodor, Mariann and Takács, Lili and Kettesy, Andrea Beáta and Kolozsvári, Bence Lajos and Nagy, Annamária and Steiber, Zita and Pásztor, Dorottya and Surányi, Éva and Széll, Noémi and Beáta, Bajdik and Balla, Szabolcs and Aranyosi, János and Mátyás, Hankovszky and Sára, Makhoul and Dorottya, Lilla Nagy and Orsolya, Pásztor and Anett, Porempovics and Ujhelyi, Bernadett}, publisher = {University of Debrecen}, unique-id = {34323209}, year = {2023} } @article{MTMT:32866680, title = {Different Effects of Cigarette Smoke, Heated Tobacco Product and E-Cigarette Vapour on Orbital Fibroblasts in Graves’ Orbitopathy; a Study by Real Time Cell Electronic Sensing}, url = {https://m2.mtmt.hu/api/publication/32866680}, author = {Aranyosi, János and Galgóczi, Erika and Erdei, Annamária and Katkó, Mónika and Fodor, Mariann and Ujhelyi, Zoltán and Bácskay, Ildikó and Nagy, Endre and Ujhelyi, Bernadett}, doi = {10.3390/molecules27093001}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {27}, unique-id = {32866680}, issn = {1431-5157}, year = {2022}, eissn = {1420-3049} } @article{MTMT:33032320, title = {Non-surgical orbital decompression using diuresis in dysthyroid optic neuropathy: a case report}, url = {https://m2.mtmt.hu/api/publication/33032320}, author = {Erdei, Annamária and Gazdag, Annamária and Ujhelyi, Bernadett and Nagy, Edit B and Berényi, Ervin László and Berta, Eszter and Steiber, Zita and Barna, Sándor Kristóf and Mezősi, Emese and Bodor, Miklós and Nagy, Endre}, doi = {10.1530/ETJ-22-0078}, journal-iso = {EUR THYROID J}, journal = {EUROPEAN THYROID JOURNAL}, volume = {11}, unique-id = {33032320}, issn = {2235-0640}, abstract = {Dysthyroid optic neuropathy (DON) is a rare, severe form of thyroid eye disease, in which decreased visual acuity is accompanied by characteristic MRI findings. The treatment of DON has always been a challenge.In a patient in whom visual acuity deteriorated on the left eye, mannitol 20% 200 ml followed by furosemide 40 mg 6 hours later, administered daily, were initiated on the day of admission. Visual function by ophthalmology methods, and orbital compartment volumes and water content by MRI were followed. Intravenous diuretics resulted in immediate therapeutic response. Visual acuity improved from 20/50 to 20/25 after two days of treatment. MRI revealed decreasing water content of both the muscle and connective tissue compartments without any volume changes. Subsequently, corticosteroids and orbital irradiation were started. Orbital decompression surgery was not required.Edematous swelling of orbital tissues is an established contributor of local pressure increase in thyroid eye disease. Diuretics reduce orbital pressure and, if confirmed by others, may be useful additions to the standard of care in sight threatening DON.}, year = {2022}, eissn = {2235-0802}, orcid-numbers = {Mezősi, Emese/0000-0001-9367-3877} }