TY - JOUR AU - Fodor, András AU - Hess, Claudia AU - Ganas, Petra AU - Boros, Zsófia AU - Kiss, János AU - Makrai, László AU - Dublecz, Károly AU - Pál, László AU - Fodor, László AU - Sebestyén, Anna AU - Klein, Michael G. AU - Tarasco, Eustachio AU - Kulkarni, Manjusha M. AU - McGwire, Bradford S. AU - Vellai, Tibor AU - Hess, Michael TI - Antimicrobial Peptides (AMP) in the Cell-Free Culture Media of Xenorhabdus budapestensis and X. szentirmaii Exert Anti-Protist Activity against Eukaryotic Vertebrate Pathogens including Histomonas meleagridis and Leishmania donovani Species JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 9 PG - 25 SN - 2079-6382 DO - 10.3390/antibiotics12091462 UR - https://m2.mtmt.hu/api/publication/34147172 ID - 34147172 N1 - Department of Genetics, Institute of Biology, Eötvös Loránd University, Pázmány Péter. sétány 1C, Budapest, H-1117, Hungary Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine (Vetmeduni Vienna), Vienna, 1210, Austria Agribiotechnology and Precision Breeding for Food Security National Laboratory, Department of Microbiology and Applied Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Páter Károly utca 1, Gödöllő, H-2100, Hungary Autovakcina Kft, Budapest, H-1171, Hungary Institute of Physiology and Nutrition, Georgikon Campus, Hungarian University of Agriculture and Life Sciences (MATE), Deák Ferenc utca 16, Keszthely, H-8360, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, H-1143, Hungary First Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, H-1085, Hungary USDA-ARS, Department of Entomology, The Ohio State University, 13416 Claremont Ave, Cleveland, OH 44130, United States Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari, 70126, Italy Division of Infectious Diseases, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, United States Export Date: 12 October 2023 Correspondence Address: Fodor, A.; Department of Genetics, Pázmány Péter. sétány 1C, Hungary; email: fodorandras@yahoo.com Correspondence Address: Hess, M.; Clinic for Poultry and Fish Medicine, Austria; email: michael.hess@vetmeduni.ac.at AB - Anti-microbial peptides provide a powerful toolkit for combating multidrug resistance. Combating eukaryotic pathogens is complicated because the intracellular drug targets in the eukaryotic pathogen are frequently homologs of cellular structures of vital importance in the host organism. The entomopathogenic bacteria (EPB), symbionts of entomopathogenic–nematode species, release a series of non-ribosomal templated anti-microbial peptides. Some may be potential drug candidates. The ability of an entomopathogenic–nematode/entomopathogenic bacterium symbiotic complex to survive in a given polyxenic milieu is a coevolutionary product. This explains that those gene complexes that are responsible for the biosynthesis of different non-ribosomal templated anti-microbial protective peptides (including those that are potently capable of inactivating the protist mammalian pathogen Leishmania donovanii and the gallinaceous bird pathogen Histomonas meleagridis) are co-regulated. Our approach is based on comparative anti-microbial bioassays of the culture media of the wild-type and regulatory mutant strains. We concluded that Xenorhabdus budapestensis and X. szentirmaii are excellent sources of non-ribosomal templated anti-microbial peptides that are efficient antagonists of the mentioned pathogens. Data on selective cytotoxicity of different cell-free culture media encourage us to forecast that the recently discovered “easy-PACId” research strategy is suitable for constructing entomopathogenic-bacterium (EPB) strains producing and releasing single, harmless, non-ribosomal templated anti-microbial peptides with considerable drug, (probiotic)-candidate potential. LA - English DB - MTMT ER - TY - JOUR AU - Ujszegi, János AU - Boros, Zsófia AU - Fodor, András AU - Vajna, Balázs AU - Hettyey, Attila TI - Metabolites of Xenorhabdus bacteria are potent candidates for mitigating amphibian chytridiomycosis JF - AMB EXPRESS J2 - AMB EXPRESS VL - 13 PY - 2023 IS - 1 SN - 2191-0855 DO - 10.1186/s13568-023-01585-0 UR - https://m2.mtmt.hu/api/publication/34111344 ID - 34111344 N1 - Department of Evolutionary Ecology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest, Hungary Department of Systematic Zoology and Ecology, Eötvös Loránd University, Budapest, Hungary Department of Genetics, Eötvös Loránd University, Budapest, Hungary Department of Microbiology, Eötvös Loránd University, Budapest, Hungary Export Date: 8 September 2023 Correspondence Address: Ujszegi, J.; Department of Systematic Zoology and Ecology, Hungary; email: ujszegi.janos@gmail.com AB - Chytridiomycosis, caused by the chytrid fungus Batrachochytrium dendrobatidis (Bd), has caused extreme losses in amphibian biodiversity. Finding bacteria that produce metabolites with antifungal properties may turn out to be invaluable in the fight against this devastating disease. The entomopathogenic bacteria, Xenorhabdus szentirmaii and X. budapestensis produce secondary metabolites that are effective against a wide range of fungal plant pathogens. To assess whether they may also be effective against Bd, we extracted cell-free culture media (CFCM) from liquid cultures of X. szentirmaii and X. budapestensis and tested their ability to inhibit Bd growth in vitro. As a second step, using juvenile common toads ( Bufo bufo ) experimentally infected with Bd we also tested the in vivo antifungal efficacy of X. szentirmaii CFCM diluted to 2 and 10% (v/v), while also assessing possible malign side effects on amphibians. Results of the in vitro experiment documented highly effective growth inhibition by CFCMs of both Xenorhabdus species. The in vivo experiment showed that treatment with CFCM of X. szentirmaii applied at a dilution of 10% resulted in infection intensities reduced by ca. 73% compared to controls and to juvenile toads treated with CFCM applied at a dilution of 2%. At the same time, we detected no negative side effects of treatment with CFCM on toad survival and development. Our results clearly support the idea that metabolites of X. szentirmaii , and perhaps of several other Xenorhabdus species as well, may prove highly useful for the treatment of Bd infected amphibians. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Vellai, Tibor AU - Hess, Claudia AU - Makrai, László AU - Dublecz, Károly AU - Pál, László AU - Molnár, Andor AU - Klein, Michael G. AU - Tarasco, Eustachio AU - Józsa, Sándor AU - Ganas, Petra AU - Hess, Michael TI - XENOFOOD—An Autoclaved Feed Supplement Containing Autoclavable Antimicrobial Peptides—Exerts Anticoccidial GI Activity, and Causes Bursa Enlargement, but Has No Detectable Harmful Effects in Broiler Cockerels despite In Vitro Detectable Cytotoxicity on LHM Cells JF - PATHOGENS J2 - PATHOGENS VL - 12 PY - 2023 IS - 3 PG - 20 SN - 2076-0817 DO - 10.3390/pathogens12030458 UR - https://m2.mtmt.hu/api/publication/33704277 ID - 33704277 N1 - Export Date: 19 April 2023 AB - Entomopathogenic bacteria are obligate symbionts of entomopathogenic nematode (EPN) species. These bacteria biosynthesize and release non-ribosomal-templated hybrid peptides (NR-AMPs), with strong, and large-spectral antimicrobial potential, capable of inactivating pathogens belonging to different prokaryote, and eukaryote taxa. The cell-free conditioned culture media (CFCM) of Xenorhabdus budapestensis and X. szentirmaii efficiently inactivate poultry pathogens like Clostridium, Histomonas, and Eimeria. To learn whether a bio-preparation containing antimicrobial peptides of Xenorhabdus origin with accompanying (in vitro detectable) cytotoxic effects could be considered a safely applicable preventive feed supplement, we conducted a 42-day feeding experiment on freshly hatched broiler cockerels. XENOFOOD (containing autoclaved X. budapestensis, and X. szentirmaii cultures developed on chicken food) were consumed by the birds. The XENOFOOD exerted detectable gastrointestinal (GI) activity (reducing the numbers of the colony-forming Clostridium perfringens units in the lower jejunum. No animal was lost in the experiment. Neither the body weight, growth rate, feed-conversion ratio, nor organ-weight data differed between the control (C) and treated (T) groups, indicating that the XENOFOOD diet did not result in any detectable adverse effects. We suppose that the parameters indicating a moderate enlargement of bursas of Fabricius (average weight, size, and individual bursa/spleen weight-ratios) in the XENOFOOD-fed group must be an indirect indication that the bursa-controlled humoral immune system neutralized the cytotoxic ingredients of the XENOFOOD in the blood, not allowing to reach their critical cytotoxic concentration in the sensitive tissues. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Clarke, David J. AU - Dillman, Adler R. AU - Tarasco, Eustachio AU - Hazir, Selcuk TI - New Antimicrobial Peptides From Bacteria/Invertebrate Obligate Symbiotic Associations JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 PG - 3 SN - 1664-302X UR - https://m2.mtmt.hu/api/publication/33871028 ID - 33871028 LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Méhi, Orsolya Katinka AU - Brivio, Maurizio TI - Editorial: Antimicrobial peptides and mRNA therapy: Clinical, Veterinary, and plant pathology perspectives with special attention to combatting MDR pathogens JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 SN - 1664-302X DO - 10.3389/fmicb.2022.1030874 UR - https://m2.mtmt.hu/api/publication/33135935 ID - 33135935 N1 - Export Date: 27 January 2023 LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Clarke, David J. AU - Dillman, Adler R. AU - Tarasco, Eustachio AU - Hazir, Selcuk TI - Editorial: New Antimicrobial Peptides From Bacteria/Invertebrate Obligate Symbiotic Associations JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 13 PY - 2022 PG - 3 SN - 1664-302X DO - 10.3389/fmicb.2022.862198 UR - https://m2.mtmt.hu/api/publication/32779279 ID - 32779279 N1 - Export Date: 3 August 2022 LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Gualtieri, Maxime AU - Zeller, Matthias AU - Tarasco, Eustachio AU - Klein, Michael G. AU - Fodor, Andrea M. AU - Haynes, Leroy AU - Lengyel, Katalin AU - Forst, Steven A. AU - Furgani, Ghazala M. AU - Karaffa, Levente AU - Vellai, Tibor TI - Type Strains of Entomopathogenic Nematode-Symbiotic Bacterium Species, Xenorhabdus szentirmaii (EMC) and X. budapestensis (EMA), Are Exceptional Sources of Non-Ribosomal Templated, Large-Target-Spectral, Thermotolerant-Antimicrobial Peptides (by Both), and Iodinin (by EMC) JF - PATHOGENS J2 - PATHOGENS VL - 11 PY - 2022 IS - 3 SN - 2076-0817 DO - 10.3390/pathogens11030342 UR - https://m2.mtmt.hu/api/publication/32742483 ID - 32742483 N1 - Department of Genetics, Eötvös University, Pázmány Péter Sétány 1/C, Budapest, H-1117, Hungary Department of Genetics, University of Szeged, Középfasor 52, Szeged, H-6726, Hungary Nosopharm, Espace Innovation 2, 110 Allée Charles Babbage, Nîmes, 30000, France Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, United States Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari, 70126, Italy Institute for Sustainable Plant Protection of CNR, Via Amendola 122/D, Bari, 70126, Italy USDA-ARS & Department of Entomology, The Ohio State University, 13416 Claremont Ave. Cleveland, Columbus, OH 44130, United States Department of Chemistry, The College of Wooster, Wooster, OH 44691, United States National Institute of Pharmacy and Nutrition (NIPN), Zrinyi utca 3, Budapest, H-1051, Hungary Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, United States Department of Plant Protection, Faculty of Agriculture, University of Tripoli, P.O. Box 13793, Tripoli, Libyan Arab Jamahiriya Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem Tér 1, Debrecen, H-4032, Hungary Institute of Metagenomics, University of Debrecen, Egyetem tér 1, Debrecen, H-4032, Hungary MTA-ELTE Genetics Research Group, Pázmány Péter Sétány 1/C, Budapest, H-1117, Hungary Export Date: 24 May 2022 Correspondence Address: Fodor, A.; Department of Genetics, Pázmány Péter Sétány 1/C, Hungary; email: fodorandras@yahoo.com AB - Antimicrobial multidrug resistance (MDR) is a global challenge, not only for public health, but also for sustainable agriculture. Antibiotics used in humans should be ruled out for use in veterinary or agricultural settings. Applying antimicrobial peptide (AMP) molecules, produced by soil-born organisms for protecting (soil-born) plants, seems a preferable alternative. The natural role of peptide-antimicrobials, produced by the prokaryotic partner of entomopathogenic-nematode/bacterium (EPN/EPB) symbiotic associations, is to sustain monoxenic conditions for the EPB in the gut of the semi-anabiotic infective dauer juvenile (IJ) EPN. They keep pathobiome conditions balanced for the EPN/EPB complex in polyxenic (soil, vanquished insect cadaver) niches. Xenorhabdus szentirmaii DSM16338(T) (EMC), and X. budapestensis DSM16342(T) (EMA), are the respective natural symbionts of EPN species Steinernema rarum and S. bicornutum. We identified and characterized both of these 15 years ago. The functional annotation of the draft genome of EMC revealed 71 genes encoding non-ribosomal peptide synthases, and polyketide synthases. The large spatial Xenorhabdus AMP (fabclavine), was discovered in EMA, and its biosynthetic pathway in EMC. The AMPs produced by EMA and EMC are promising candidates for controlling MDR prokaryotic and eukaryotic pathogens (bacteria, oomycetes, fungi, protozoa). EMC releases large quantity of iodinin (1,6-dihydroxyphenazine 5,10-dioxide) in a water-soluble form into the media, where it condenses to form spectacular water-insoluble, macroscopic crystals. This review evaluates the scientific impact of international research on EMA and EMC. LA - English DB - MTMT ER - TY - JOUR AU - Fodor, András AU - Abate, Birhan Addisie AU - Deák, Péter AU - Fodor, László AU - Gyenge, Ervin AU - Klein, Michael G. AU - Koncz, Zsuzsanna AU - Muvevi, Josephat AU - Ötvös, László AU - Székely, Gyöngyi AU - Vozik, Dávid AU - Makrai, László TI - Multidrug Resistance (MDR) and Collateral Sensitivity in Bacteria, with Special Attention to Genetic and Evolutionary Aspects and to the Perspectives of Antimicrobial Peptides—A Review JF - PATHOGENS J2 - PATHOGENS VL - 9 PY - 2020 IS - 7 PG - 53 SN - 2076-0817 DO - 10.3390/pathogens9070522 UR - https://m2.mtmt.hu/api/publication/31364575 ID - 31364575 N1 - Department of Genetics, University of Szeged, Szeged, H-6726, Hungary Ethiopian Biotechnology Institute, Agricultural Biotechnology Directorate, Addis Ababa, 5954, Ethiopia Institute of Biochemistry, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest, H-1581, Hungary Hungarian Department of Biology and Ecology, Faculty of Biology and Geology, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Institute for Research-Development-Innovation in Applied Natural Sciences, Babeș-Bolyai University, 30 Fântânele St., Cluj-Napoca, 400294, Romania Department of Entomology, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, United States Max-Planck Institut für Pflanzenzüchtungsforschung, Carl-von-Linné-Weg 10, Köln, D-50829, Germany National Cereals and Produce Board, Mombasa, 80100, Kenya OLPE, LLC, Audubon, PA 19403-1965, United States Institute of Medical Microbiology, Semmelweis University, Budapest, H-1085, Hungary Arrevus, Inc., Raleigh, NC 27612, United States Centre for Systems Biology, Biodiversity and Bioresources, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Research Institute on Bioengineering, Membrane Technology and Energetics, Faculty of Engineering, University of Veszprem, Veszprém, H-8200, Hungary Export Date: 24 August 2020 Correspondence Address: Fodor, A.; Department of Genetics, University of Szeged, Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Hungary; email: fodora@expbio.bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, H-6726, Hungary Ethiopian Biotechnology Institute, Agricultural Biotechnology Directorate, Addis Ababa, 5954, Ethiopia Institute of Biochemistry, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest, H-1581, Hungary Hungarian Department of Biology and Ecology, Faculty of Biology and Geology, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Institute for Research-Development-Innovation in Applied Natural Sciences, Babeș-Bolyai University, 30 Fântânele St., Cluj-Napoca, 400294, Romania Department of Entomology, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, United States Max-Planck Institut für Pflanzenzüchtungsforschung, Carl-von-Linné-Weg 10, Köln, D-50829, Germany National Cereals and Produce Board, Mombasa, 80100, Kenya OLPE, LLC, Audubon, PA 19403-1965, United States Institute of Medical Microbiology, Semmelweis University, Budapest, H-1085, Hungary Arrevus, Inc., Raleigh, NC 27612, United States Centre for Systems Biology, Biodiversity and Bioresources, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Research Institute on Bioengineering, Membrane Technology and Energetics, Faculty of Engineering, University of Veszprem, Veszprém, H-8200, Hungary Export Date: 8 December 2020 Correspondence Address: Fodor, A.; Department of Genetics, University of Szeged, Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Hungary; email: fodora@expbio.bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, H-6726, Hungary Ethiopian Biotechnology Institute, Agricultural Biotechnology Directorate, Addis Ababa, 5954, Ethiopia Institute of Biochemistry, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest, H-1581, Hungary Hungarian Department of Biology and Ecology, Faculty of Biology and Geology, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Institute for Research-Development-Innovation in Applied Natural Sciences, Babeș-Bolyai University, 30 Fântânele St., Cluj-Napoca, 400294, Romania Department of Entomology, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, United States Max-Planck Institut für Pflanzenzüchtungsforschung, Carl-von-Linné-Weg 10, Köln, D-50829, Germany National Cereals and Produce Board, Mombasa, 80100, Kenya OLPE, LLC, Audubon, PA 19403-1965, United States Institute of Medical Microbiology, Semmelweis University, Budapest, H-1085, Hungary Arrevus, Inc., Raleigh, NC 27612, United States Centre for Systems Biology, Biodiversity and Bioresources, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Research Institute on Bioengineering, Membrane Technology and Energetics, Faculty of Engineering, University of Veszprem, Veszprém, H-8200, Hungary Cited By :1 Export Date: 14 January 2021 Correspondence Address: Fodor, A.; Department of Genetics, University of Szeged, Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Hungary; email: fodora@expbio.bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, H-6726, Hungary Ethiopian Biotechnology Institute, Agricultural Biotechnology Directorate, Addis Ababa, 5954, Ethiopia Institute of Biochemistry, Biological Research Centre, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest, H-1581, Hungary Hungarian Department of Biology and Ecology, Faculty of Biology and Geology, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Institute for Research-Development-Innovation in Applied Natural Sciences, Babeș-Bolyai University, 30 Fântânele St., Cluj-Napoca, 400294, Romania Department of Entomology, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, United States Max-Planck Institut für Pflanzenzüchtungsforschung, Carl-von-Linné-Weg 10, Köln, D-50829, Germany National Cereals and Produce Board, Mombasa, 80100, Kenya OLPE, LLC, Audubon, PA 19403-1965, United States Institute of Medical Microbiology, Semmelweis University, Budapest, H-1085, Hungary Arrevus, Inc., Raleigh, NC 27612, United States Centre for Systems Biology, Biodiversity and Bioresources, Babeș-Bolyai University, 5-7 Clinicilor St., Cluj-Napoca, 400006, Romania Research Institute on Bioengineering, Membrane Technology and Energetics, Faculty of Engineering, University of Veszprem, Veszprém, H-8200, Hungary Cited By :10 Export Date: 25 August 2021 Correspondence Address: Fodor, A.; Department of Genetics, Hungary; email: fodora@expbio.bio.u-szeged.hu Correspondence Address: Makrai, L.; Department of Microbiology and Infectious Diseases, P.O. Box 22, Hungary; email: Makrai.Laszlo@univet.hu LA - English DB - MTMT ER - TY - GEN AU - Fodor, András AU - Birhan, Addisie Abate AU - Deák, Péter AU - László, Fodor AU - Michael, G. Klein AU - László, Makrai AU - Josephat, Muvevi AU - Dávid, Vozik TI - An Overview of Multi-Antibiotic Resistance in Pathogenic Bacteria - From Selected Genetic and Evolutionary Aspects - A Review ET - 0 PY - 2018 SP - 1 EP - 33 PG - 33 DO - 10.20944/preprints201808.0036.v1 UR - https://m2.mtmt.hu/api/publication/30355860 ID - 30355860 LA - English DB - MTMT ER - TY - JOUR AU - El-Deen, A. H. Nour AU - Fodor, András AU - El-Barty, Amal F. TI - Nematicidal activity of entomopathogenic bacteria against root-knot nematodes, Meloidogyne incognita in-vitro JF - INTERNATIONAL JOURNAL OF ADVANCED RESEARCH J2 - INT J ADV RES VL - 2 PY - 2014 IS - 6 SP - 708 EP - 713 PG - 6 SN - 2320-5407 UR - https://m2.mtmt.hu/api/publication/34560862 ID - 34560862 LA - English DB - MTMT ER -