TY - CONF AU - Mozaffaritabar, Soroosh AU - Zhou, Lei AU - Koltai, Erika AU - Radák, Zsolt TI - PGC-1α activation boots exercise-dependent cellular response in the skeletal muscle T2 - IX. SPORTTUDOMÁNYI PHD SZIMPÓZIUM PY - 2024 SP - 51 EP - 51 PG - 1 UR - https://m2.mtmt.hu/api/publication/35191347 ID - 35191347 LA - English DB - MTMT ER - TY - JOUR AU - György, Bernadett AU - Pálóczi, Krisztina AU - Balbisi, Mirjam AU - Turiák, Lilla AU - Drahos, László AU - Visnovitz, Tamás AU - Koltai, Erika AU - Radák, Zsolt TI - Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles JF - CURRENT ISSUES IN MOLECULAR BIOLOGY J2 - CURR ISSUES MOL BIOL VL - 46 PY - 2024 IS - 5 SP - 4337 EP - 4357 PG - 21 SN - 1467-3037 DO - 10.3390/cimb46050264 UR - https://m2.mtmt.hu/api/publication/34850199 ID - 34850199 N1 - Research Centre for Molecular Exercise Science, Hungarian University of Sport Science, Alkotás u. 42-48, Budapest, 1123, Hungary Department of Genetics, Cell and Immunobiology, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary Research Centre for Natural Sciences, Institute of Organic Chemistry, Magyar Tudósok Körútja 2, Budapest, 1117, Hungary Department of Plant Physiology and Molecular Plant Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/c, Budapest, 1117, Hungary Faculty of Sport Sciences, Waseda University, Tokorozawa 2-579-15, Japan Export Date: 17 June 2024 CODEN: CMBIF Correspondence Address: Radák, Z.; Research Centre for Molecular Exercise Science, Alkotás u. 42-48, Hungary; email: radak.zsolt@tf.hu AB - The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (>2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials. LA - English DB - MTMT ER - TY - JOUR AU - Torma, Ferenc Gergely AU - Kerepesi, Csaba AU - Jókai, Mátyás AU - Bábszky, Gergely AU - Koltai, Erika AU - Ligeti, Balázs AU - Kalcsevszki, Regina AU - McGreevy, Kristen M. AU - Horvath, Steve AU - Radák, Zsolt TI - Alterations of the gut microbiome are associated with epigenetic age acceleration and physical fitness JF - AGING CELL J2 - AGING CELL VL - 23 PY - 2024 IS - 4 PG - 12 SN - 1474-9718 DO - 10.1111/acel.14101 UR - https://m2.mtmt.hu/api/publication/34693196 ID - 34693196 N1 - Funding Agency and Grant Number: European Union; National Research, Development and Innovation Office, Hungary; National Excellence Program [126823]; National Science and Research Found [OTKA142192, TKP2021-EGA-37]; Hungarian University Sport Science, Innovation and Technology Ministry, Hungary [2021-28]; National Academy of Science, Hungary; [RRF-2.3.1-21-2022-00004]; [OTKA146113] Funding text: CK was supported by the European Union project RRF-2.3.1-21-2022-00004 within the framework of the Artificial Intelligence National Laboratory, and the National Excellence Program (OTKA146113) of the National Research, Development and Innovation Office, Hungary. ZR acknowledges support from the National Excellence Program (126823) National Science and Research Found (OTKA142192) and Scientific Excellence Program TKP2021-EGA-37 at the Hungarian University Sport Science, Innovation and Technology Ministry, Hungary, as well as Post-Covid 2021-28 grant by National Academy of Science, Hungary. We thank to Szilvia Farkas (Semmelweis University, Hungary) for the valuable discussions. AB - Epigenetic clocks can measure aging and predict the incidence of diseases and mortality. Higher levels of physical fitness are associated with a slower aging process and a healthier lifespan. Microbiome alterations occur in various diseases and during the aging process, yet their relation to epigenetic clocks is not explored. To fill this gap, we collected metagenomic (from stool), epigenetic (from blood), and exercise‐related data from physically active individuals and, by applying epigenetic clocks, we examined the relationship between gut flora, blood‐based epigenetic age acceleration, and physical fitness. We revealed that an increased entropy in the gut microbiome of physically active middle‐aged/old individuals is associated with accelerated epigenetic aging, decreased fitness, or impaired health status. We also observed that a slower epigenetic aging and higher fitness level can be linked to altered abundance of some bacterial species often linked to anti‐inflammatory effects. Overall our data suggest that alterations in the microbiome can be associated with epigenetic age acceleration and physical fitness. LA - English DB - MTMT ER - TY - JOUR AU - Mozaffaritabar, Soroosh AU - Koltai, Erika AU - Zhou, Lei AU - Bori, Zoltán AU - Kolonics, Attila AU - Kujach, Sylwester AU - Gu, Yaodong AU - Koike, Atsuko AU - Boros, Anita AU - Radák, Zsolt TI - PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle JF - JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY J2 - J PHYSIOL BIOCHEM VL - 80 PY - 2024 IS - 2 SP - 329 EP - 335 PG - 7 SN - 1138-7548 DO - 10.1007/s13105-024-01006-1 UR - https://m2.mtmt.hu/api/publication/34539479 ID - 34539479 N1 - Received25 August 2023 Accepted10 January 2024 Published23 January 2024 AB - The role of Peroxisome proliferator-activated receptor-gamma coactivator alpha (PGC-1α) in fat metabolism is not well known. In this study, we compared the mechanisms of muscle-specific PGC-1α overexpression and exercise-related adaptation-dependent fat metabolism. PGC-1α trained (PGC-1α Ex) and wild-trained (wt-ex) mice were trained for 10 weeks, five times a week at 30 min per day with 60 percent of their maximal running capacity. The PGC-1α overexpressed animals exhibited higher levels of Fibronectin type III domain-containing protein 5 (FNDC5), 5' adenosine monophosphate-activated protein kinase alpha (AMPK-α), the mammalian target of rapamycin (mTOR), Sirtuin 1 (SIRT1), Lon protease homolog 1 (LONP1), citrate synthase (CS), succinate dehydrogenase complex flavoprotein subunit A (SDHA), Mitofusin-1 (Mfn1), endothelial nitric oxide synthase (eNOS), Hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), G protein-coupled receptor 41 (GPR41), and Phosphatidylcholine Cytidylyltransferase 2 (PCYT2), and lower levels of Sirtuin 3 (SIRT3) compared to wild-type animals. Exercise training increased the protein content levels of SIRT1, HSL, and ATGL in both the wt-ex and PGC-1α trained groups. PGC-1α has a complex role in cellular signaling, including the upregulation of lipid metabolism-associated proteins. Our data reveals that although exercise training mimics the effects of PGC-1α overexpression, it incorporates some PGC-1α-independent adaptive mechanisms in fat uptake and cell signaling. LA - English DB - MTMT ER - TY - CONF AU - Soroosh, Mozafffaritabar AU - Koltai, Erika AU - Radák, Zsolt TI - The role of PGC1-A in inter-muscular lipid metabolism in exercised mice T2 - VIII. Sporttudományi PhD Szimpózium PB - Magyar Testnevelési és Sporttudományi Egyetem C1 - Budapest PY - 2023 SP - 47 EP - 48 PG - 2 UR - https://m2.mtmt.hu/api/publication/34053803 ID - 34053803 LA - English DB - MTMT ER - TY - JOUR AU - Jókai, Mátyás AU - Torma, Ferenc Gergely AU - McGreevy, Kristen M. AU - Koltai, Erika AU - Bori, Zoltán AU - Bábszky, Gergely AU - Bakonyi, Péter AU - Gombos, Zoltán AU - György, Bernadett AU - Aczél, Dóra Tímea AU - Tóth, László AU - Osváth, Péter AU - Fridvalszki, Marcell Norbert AU - Téglás, Tímea AU - Pósa, Anikó AU - Kujach, Sylwester AU - Olek, Robert AU - Kawamura, Takuji AU - Seki, Yasuhiro AU - Suzuki, Katsuhiko AU - Tanisawa, Kumpei AU - Goto, Sataro AU - Kerepesi, Csaba AU - Boldogh, Istvan AU - Ba, Xueqing AU - Davies, Kelvin J. A. AU - Horvath, Steve AU - Radák, Zsolt TI - DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 45 PY - 2023 IS - 5 SP - 2805 EP - 2817 PG - 13 SN - 2509-2715 DO - 10.1007/s11357-023-00826-1 UR - https://m2.mtmt.hu/api/publication/33866054 ID - 33866054 AB - DNAmPhenoAge, DNAmGrimAge, and the newly developed DNAmFitAge are DNA methylation (DNAm)-based biomarkers that reflect the individual aging process. Here, we examine the relationship between physical fitness and DNAm-based biomarkers in adults aged 33–88 with a wide range of physical fitness (including athletes with long-term training history). Higher levels of VO 2 max ( ρ = 0.2, p = 6.4E − 4, r = 0.19, p = 1.2E − 3), Jumpmax ( p = 0.11, p = 5.5E − 2, r = 0.13, p = 2.8E − 2), Gripmax ( ρ = 0.17, p = 3.5E − 3, r = 0.16, p = 5.6E − 3), and HDL levels ( ρ = 0.18, p = 1.95E − 3, r = 0.19, p = 1.1E − 3) are associated with better verbal short-term memory. In addition, verbal short-term memory is associated with decelerated aging assessed with the new DNAm biomarker FitAgeAcceleration ( ρ : − 0.18, p = 0.0017). DNAmFitAge can distinguish high-fitness individuals from low/medium-fitness individuals better than existing DNAm biomarkers and estimates a younger biological age in the high-fit males and females (1.5 and 2.0 years younger, respectively). Our research shows that regular physical exercise contributes to observable physiological and methylation differences which are beneficial to the aging process. DNAmFitAge has now emerged as a new biological marker of quality of life. LA - English DB - MTMT ER - TY - JOUR AU - Cikes, Domagoj AU - Elsayad, Kareem AU - Sezgin, Erdinc AU - Koltai, Erika AU - Torma, Ferenc Gergely AU - Orthofer, Michael AU - Yarwood, Rebecca AU - Heinz, Leonhard X. AU - Sedlyarov, Vitaly AU - Miranda, Nasser Darwish AU - Taylor, Adrian AU - Grapentine, Sophie AU - al-Murshedi, Fathiya AU - Abot, Anne AU - Weidinger, Adelheid AU - Kutchukian, Candice AU - Sanchez, Colline AU - Cronin, Shane J. F. AU - Novatchkova, Maria AU - Kavirayani, Anoop AU - Schuetz, Thomas AU - Haubner, Bernhard AU - Haas, Lisa AU - Hagelkruys, Astrid AU - Jackowski, Suzanne AU - Kozlov, Andrey V. AU - Jacquemond, Vincent AU - Knauf, Claude AU - Superti-Furga, Giulio AU - Rullman, Eric AU - Gustafsson, Thomas AU - McDermot, John AU - Lowe, Martin AU - Radák, Zsolt AU - Chamberlain, Jeffrey S. AU - Bakovic, Marica AU - Banka, Siddharth AU - Penninger, Josef M. TI - Author Correction: PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing JF - NATURE METABOLISM J2 - NAT METAB VL - 2023 PY - 2023 IS - 5 SP - 495 SN - 2522-5812 DO - 10.1038/s42255-023-00791-1 UR - https://m2.mtmt.hu/api/publication/33745909 ID - 33745909 LA - English DB - MTMT ER - TY - JOUR AU - Cikes, Domagoj AU - Elsayad, Kareem AU - Sezgin, Erdinc AU - Koltai, Erika AU - Torma, Ferenc Gergely AU - Orthofer, Michael AU - Yarwood, Rebecca AU - Heinz, Leonhard X. AU - Sedlyarov, Vitaly AU - Miranda, Nasser Darwish AU - Taylor, Adrian AU - Grapentine, Sophie AU - al-Murshedi, Fathiya AU - Abot, Anne AU - Weidinger, Adelheid AU - Kutchukian, Candice AU - Sanchez, Colline AU - Cronin, Shane J. F. AU - Novatchkova, Maria AU - Kavirayani, Anoop AU - Schuetz, Thomas AU - Haubner, Bernhard AU - Haas, Lisa AU - Hagelkruys, Astrid AU - Jackowski, Suzanne AU - Kozlov, Andrey AU - Jacquemond, Vincent AU - Knauf, Claude AU - Superti-Furga, Giulio AU - Rullman, Eric AU - Gustafsson, Thomas AU - McDermot, John AU - Lowe, Martin AU - Radák, Zsolt AU - Chamberlain, Jeffrey S. AU - Bakovic, Marica AU - Banka, Siddharth AU - Penninger, Josef M. TI - PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing JF - NATURE METABOLISM J2 - NAT METAB VL - 5 PY - 2023 IS - 3 SP - 495 EP - 515 PG - 20 SN - 2522-5812 DO - 10.1038/s42255-023-00766-2 UR - https://m2.mtmt.hu/api/publication/33712726 ID - 33712726 N1 - Received 08 February 2021, Accepted 10 February 2023, Published 20 March 2023 AB - Muscle degeneration is the most prevalent cause for frailty and dependency in inherited diseases and ageing. Elucidation of pathophysiological mechanisms, as well as effective treatments for muscle diseases, represents an important goal in improving human health. Here, we show that the lipid synthesis enzyme phosphatidylethanolamine cytidyltransferase (PCYT2/ECT) is critical to muscle health. Human deficiency in PCYT2 causes a severe disease with failure to thrive and progressive weakness. pcyt2-mutant zebrafish and muscle-specific Pcyt2-knockout mice recapitulate the participant phenotypes, with failure to thrive, progressive muscle weakness and accelerated ageing. Mechanistically, muscle Pcyt2 deficiency affects cellular bioenergetics and membrane lipid bilayer structure and stability. PCYT2 activity declines in ageing muscles of mice and humans, and adeno-associated virus-based delivery of PCYT2 ameliorates muscle weakness in Pcyt2-knockout and old mice, offering a therapy for individuals with a rare disease and muscle ageing. Thus, PCYT2 plays a fundamental and conserved role in vertebrate muscle health, linking PCYT2 and PCYT2-synthesized lipids to severe muscle dystrophy and ageing. LA - English DB - MTMT ER - TY - CONF AU - György, Bernadett AU - Pálóczi, Krisztina AU - Buzás, Edit AU - Koltai, Erika AU - Radák, Zsolt TI - A több évtizedes testedzés hatása a humán vérből izolált extracelluláris vezikulák tartalmára T2 - VII. SPORTTUDOMÁNYI PHD SZIMPÓZIUM - PROGRAM- ÉS ABSZTRAKTFÜZET PY - 2022 SP - 24 EP - 24 PG - 1 UR - https://m2.mtmt.hu/api/publication/33022430 ID - 33022430 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ireland, Alex AU - Mittag, Uwe AU - Degens, Hans AU - Felsenberg, Dieter AU - Heinonen, Ari AU - Koltai, Erika AU - Korhonen, Marko T. AU - McPhee, Jamie S. AU - Mekjavic, Igor AU - Pisot, Rado AU - Rawer, Rainer AU - Radák, Zsolt AU - Simunic, Bostjan AU - Suominen, Harri AU - Rittweger, Jörn TI - Age-Related Declines in Lower Limb Muscle Function are Similar in Power and Endurance Athletes of Both Sexes: A Longitudinal Study of Master Athletes JF - CALCIFIED TISSUE INTERNATIONAL J2 - CALCIFIED TISSUE INT VL - 110 PY - 2022 IS - 2 SP - 196 EP - 203 PG - 8 SN - 0171-967X DO - 10.1007/s00223-021-00907-3 UR - https://m2.mtmt.hu/api/publication/32259162 ID - 32259162 N1 - Received12 April 2021; Accepted18 August 2021; Published09 September 2021 LA - English DB - MTMT ER -