@article{MTMT:34819821,
	title = {In situ captured antibacterial action of membrane-incising peptide lamellae},
	url = {https://m2.mtmt.hu/api/publication/34819821},
	author = {el Battioui, Kamal and Chakraborty, Sohini and Wacha, András and Molnár, Dániel and Quemé-Peña, Mayra and Szigyártó, Imola Cs. and Szabó, Csenge Lilla and Bodor, Andrea and Horváti, Kata and Gyulai, Gergő and Bősze, Szilvia and Mihály, Judith and Jezsó, Bálint and Románszki, Loránd and Tóth, Judit and Varga, Zoltán and Mándity, István and Juhász, Tünde and Beke-Somfai, Tamás},
	doi = {10.1038/s41467-024-47708-4},
	journal-iso = {NAT COMMUN},
	journal = {NATURE COMMUNICATIONS},
	volume = {15},
	unique-id = {34819821},
	issn = {2041-1723},
	abstract = {Developing unique mechanisms of action are essential to combat the growing issue of antimicrobial resistance. Supramolecular assemblies combining the improved biostability of non-natural compounds with the complex membrane-attacking mechanisms of natural peptides are promising alternatives to conventional antibiotics. However, for such compounds the direct visual insight on antibacterial action is still lacking. Here we employ a design strategy focusing on an inducible assembly mechanism and utilized electron microscopy (EM) to follow the formation of supramolecular structures of lysine-rich heterochiral β 3 -peptides, termed lamellin-2K and lamellin-3K, triggered by bacterial cell surface lipopolysaccharides. Combined molecular dynamics simulations, EM and bacterial assays confirmed that the phosphate-induced conformational change on these lamellins led to the formation of striped lamellae capable of incising the cell envelope of Gram-negative bacteria thereby exerting antibacterial activity. Our findings also provide a mechanistic link for membrane-targeting agents depicting the antibiotic mechanism derived from the in-situ formation of active supramolecules.},
	year = {2024},
	eissn = {2041-1723},
	orcid-numbers = {Wacha, András/0000-0002-9609-0893; Szabó, Csenge Lilla/0000-0002-6508-3439; Bodor, Andrea/0000-0002-7422-298X; Gyulai, Gergő/0000-0002-1352-2014; Jezsó, Bálint/0000-0002-1306-4797; Románszki, Loránd/0000-0002-6347-5228; Tóth, Judit/0000-0002-0965-046X; Varga, Zoltán/0000-0002-5741-2669; Mándity, István/0000-0003-2865-6143; Beke-Somfai, Tamás/0000-0002-4788-3758}
}

@article{MTMT:34567532,
	title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches},
	url = {https://m2.mtmt.hu/api/publication/34567532},
	author = {Welsh, Joshua A. and Goberdhan, Deborah C. I. and O'Driscoll, Lorraine and Buzás, Edit Irén and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Di Vizio, Dolores and Driedonks, Tom A. P. and Erdbrügger, Uta and Falcon‐Perez, Juan M. and Fu, Qing‐Ling and Hill, Andrew F. and Lenassi, Metka and Lim, Sai Kiang and Mahoney, Mỹ G. and Mohanty, Sujata and Möller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C. and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M. and Brucale, Marco and Burger, Dylan and Carney, Randy P. and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L. and Haughey, Norman J. and Hendrix, An and Ivanov, Alexander R. and Jovanovic‐Talisman, Tijana and Kruh‐Garcia, Nicole A. and Ku'ulei‐Lyn Faustino, Vroniqa and Kyburz, Diego and Lässer, Cecilia and Lennon, Kathleen M. and Lötvall, Jan and Maddox, Adam L. and Martens‐Uzunova, Elena S. and Mizenko, Rachel R. and Newman, Lauren A. and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S. and Saugstad, Julie A. and Shekari, Faezeh and Swift, Simon and Ter‐Ovanesyan, Dmitry and Tosar, Juan P. and Useckaite, Zivile and Valle, Francesco and Varga, Zoltán and van der Pol, Edwin and van Herwijnen, Martijn J. C. and Wauben, Marca H. M. and Wehman, Ann M. and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J. and Théry, Clotilde and Witwer, Kenneth W. and Haseeb, Zubair},
	doi = {10.1002/jev2.12404},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {13},
	unique-id = {34567532},
	abstract = {Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.},
	year = {2024},
	eissn = {2001-3078},
	orcid-numbers = {Welsh, Joshua A./0000-0002-1097-9756; Goberdhan, Deborah C. I./0000-0003-0645-6714; Buzás, Edit Irén/0000-0002-3744-206X; Bussolati, Benedetta/0000-0002-3663-5134; Cai, Houjian/0000-0003-4887-2652; Falcon‐Perez, Juan M./0000-0003-3133-0670; Hill, Andrew F./0000-0001-5581-2354; Lenassi, Metka/0000-0002-9488-6855; Mohanty, Sujata/0000-0002-0047-4914; Nieuwland, Rienk/0000-0002-5671-3400; Ochiya, Takahiro/0000-0002-0776-9918; Sahoo, Susmita/0000-0002-7279-1564; Torrecilhas, Ana C./0000-0001-5724-2199; Zheng, Lei/0000-0003-2576-8780; Zijlstra, Andries/0000-0001-8460-8803; Brucale, Marco/0000-0001-7244-4389; Carney, Randy P./0000-0001-8193-1664; Crescitelli, Rossella/0000-0002-1714-3169; Haughey, Norman J./0000-0001-5194-4122; Martens‐Uzunova, Elena S./0000-0002-5363-2525; Newman, Lauren A./0000-0003-3303-1666; Rohde, Eva/0000-0001-8692-886X; Sandau, Ursula S./0000-0002-3646-7089; Saugstad, Julie A./0000-0002-2996-9611; Shekari, Faezeh/0000-0001-6026-5412; Tosar, Juan P./0000-0002-2021-2479; Varga, Zoltán/0000-0002-5741-2669; Wauben, Marca H. M./0000-0003-0360-0311; Wehman, Ann M./0000-0001-9826-4132; Zimmerman, Alan J./0000-0001-6280-4790; Théry, Clotilde/0000-0001-8294-6884; Witwer, Kenneth W./0000-0003-1664-4233; Bodnár, Bernadett Réka/0000-0003-3347-9225; Bukva, Mátyás/0000-0002-5225-0285; Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Dobra, Gabriella/0000-0002-2814-7720; Försönits, András/0000-0002-9298-8890; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Márton Ákos/0000-0002-2819-5116; Németh, Krisztina/0000-0002-3825-2137; Oláh, Attila/0000-0003-4122-5639; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Stepanova, Ganna/0000-0002-8285-2762; Visnovitz, Tamás/0000-0002-7962-5083; Wiener, Zoltán/0000-0001-7056-4926; Harmati, Mária/0000-0002-4875-5723; Hegyesi, Hargita/0000-0002-8800-5169}
}

@article{MTMT:34554364,
	title = {Traceable characterization of hollow organosilica beads as potential reference materials for extracellular vesicle measurements with optical techniques},
	url = {https://m2.mtmt.hu/api/publication/34554364},
	author = {Deumer, J. and Schürmann, R. and Gaál, Anikó and Varga, Zoltán and Bettin, B. and van, der Pol E. and Nieuwland, R. and Ojeda, D. and Sikora, A. and Bartczak, D. and Goenaga-Infante, H. and Noireaux, J. and Khakpour, M. and Korpelainen, V. and Gollwitzer, C.},
	doi = {10.1186/s11671-024-03956-3},
	journal-iso = {DISCOVER NANO},
	journal = {DISCOVER NANO},
	volume = {19},
	unique-id = {34554364},
	abstract = {The concentration of cell-type specific extracellular vesicles (EVs) is a promising biomarker for various diseases. However, concentrations of EVs measured by optical techniques such as flow cytometry (FCM) or particle tracking analysis (PTA) in clinical practice are incomparable. To allow reliable and comparable concentration measurements suitable reference materials (RMs) and SI-traceable (SI—International system of units) methods are required. Hollow organosilica beads (HOBs) are promising RM candidates for concentration measurements of EVs based on light scattering, as the shape, low refractive index, and number concentration of HOBs are comparable to EVs of the respective size range that can be detected with current optical instrumentation. Here, we present traceable methods for measuring the particle size distribution of four HOB types in the size range between 200 and 500 nm by small-angle X-ray scattering (SAXS) and atomic force microscopy (AFM), as well as the number concentration by single-particle inductively coupled plasma mass spectrometry (spICP-MS). Based on the size and shape results, traceable reference values were obtained to additionally determine the refractive index of the shell of the HOB samples by FCM. Furthermore, the estimated refractive indexes of the HOBs plausibly agree with the refractive indexes of EVs of corresponding size. Due to their narrow size distribution and their similar shape, and low refractive index, all HOB samples studied are suitable RM candidates for calibration of the measured sample volume by optical methods within the photon wavelength range used, and thus for calibration of number concentration measurements of EVs in the size range indicated. This was confirmed as the number concentration values obtained by PTA and two independent flow cytometric measurements agreed with the concentration reference values obtained by two independent spICP-MS measurements within the calculated uncertainty limits. © 2024, The Author(s).},
	keywords = {SILICA; CALIBRATION; size distribution; Flow Cytometry; Flow Cytometry; Mass spectrometry; uncertainty analysis; particle size; light scattering; Particle size analysis; Reference material; Extracellular; X ray scattering; Refractive index; particle tracking; extracellular vesicle; Extracellular vesicles; Number concentration; Organosilicas; reference materials; particle tracking analysis; Nanometrology; Nanometrology; tracking analysis; Hollow organosilica beads; Hollow organosilicum bead; Particle tracking analyse},
	year = {2024},
	eissn = {2731-9229},
	orcid-numbers = {Gaál, Anikó/0000-0003-4064-1825; Varga, Zoltán/0000-0002-5741-2669}
}

@CONFERENCE{MTMT:34627971,
	title = {Systematic investigation and classification of membrane active peptide peptides based on their affinity for interaction with extracellular vesicles},
	url = {https://m2.mtmt.hu/api/publication/34627971},
	author = {Tasvilla, Sonallya and Szigyártó, Imola Csilla and Juhász, Tünde and Edit, I. Buzas and Delaram, Khamari and Kinga, Ilyes and Varga, Zoltán and Beke-Somfai, Tamás},
	booktitle = {Small New World 2.0},
	unique-id = {34627971},
	year = {2023},
	pages = {63},
	orcid-numbers = {Varga, Zoltán/0000-0002-5741-2669}
}

@article{MTMT:34443931,
	title = {Radiolabeling of Platelets with 99mTc-HYNIC-Duramycin for In Vivo Imaging Studies},
	url = {https://m2.mtmt.hu/api/publication/34443931},
	author = {Merkel, Keresztély Géza and Szöllősi, Dávid and Horváth, Ildikó and Jezsó, Bálint and Baranyai, Zsolt and Szigeti, Krisztián and Varga, Zoltán and Hegedüs, Imre and Padmanabhan, P. and Gulyás, B. and Bergmann, Ralf Konrad and Máthé, Domokos},
	doi = {10.3390/ijms242317119},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34443931},
	issn = {1661-6596},
	year = {2023},
	eissn = {1422-0067},
	orcid-numbers = {Szöllősi, Dávid/0000-0002-3363-3862; Jezsó, Bálint/0000-0002-1306-4797; Varga, Zoltán/0000-0002-5741-2669; Hegedüs, Imre/0000-0002-1438-3511}
}

@article{MTMT:34267696,
	title = {Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display},
	url = {https://m2.mtmt.hu/api/publication/34267696},
	author = {Szöllősi, Dávid and Hajdrik, Polett and Tordai, Hedvig and Horváth, Ildikó and Veres, Dániel and Gillich, Bernadett and Shailaja, Kanni Das and Smeller, László and Bergmann, Ralf Konrad and Bachmann, Michael and Mihály, Judith and Gaál, Anikó and Jezsó, Bálint and Barátki, Balázs Lajos and Kövesdi, Dorottya and Bősze, Szilvia and Szabó, Ildikó and Felföldi, Tamás and Oszwald, Erzsébet and Padmanabhan, Parasuraman and Gulyás, Balázs Zoltán and Hamdani, Nazha and Máthé, Domokos and Varga, Zoltán and Szigeti, Krisztián},
	doi = {10.1038/s41598-023-45628-9},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34267696},
	issn = {2045-2322},
	abstract = {The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain ( E. coli BL21(DE3) ΔnlpI, ΔlpxM ) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64 Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64 Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Szöllősi, Dávid/0000-0002-3363-3862; Hajdrik, Polett/0000-0002-5452-4892; Tordai, Hedvig/0000-0002-0875-5569; Veres, Dániel/0000-0002-9687-3556; Smeller, László/0000-0002-3643-3268; Gaál, Anikó/0000-0003-4064-1825; Jezsó, Bálint/0000-0002-1306-4797; Szabó, Ildikó/0000-0002-9844-7841; Felföldi, Tamás/0000-0003-2009-2478; Hamdani, Nazha/0000-0002-3053-0008; Varga, Zoltán/0000-0002-5741-2669}
}

@CONFERENCE{MTMT:34207881,
	title = {New Perspectives in the removal of protein Corona: host define peptides as useful tools in EV surface engineering},
	url = {https://m2.mtmt.hu/api/publication/34207881},
	author = {Beke-Somfai, Tamás and Szigyártó, Imola Csilla and Varga, Zoltán and Mihály, Judith and Singh, Priyanka and Ricci, Maria and Gaál, Anikó and Quemé Peña, Mayra and Kitka, Diána and Fülöp, L and Drahos, L},
	booktitle = {ISEV2023 Abstract Book},
	unique-id = {34207881},
	year = {2023},
	orcid-numbers = {Varga, Zoltán/0000-0002-5741-2669; Gaál, Anikó/0000-0003-4064-1825}
}

@article{MTMT:34140818,
	title = {The interaction between mucin and poly(amino acid)s with controlled cationic group content in bulk phase and in thin layers},
	url = {https://m2.mtmt.hu/api/publication/34140818},
	author = {Stankovits, József Gergely and Ábrahám, Ágnes and Kiss, Éva and Varga, Zoltán and Misra, Anil and Szilágyi, András Ferenc and Gyarmati, Benjámin Sándor},
	doi = {10.1016/j.ijbiomac.2023.126826},
	journal-iso = {INT J BIOL MACROMOL},
	journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
	volume = {253},
	unique-id = {34140818},
	issn = {0141-8130},
	abstract = {The type and concentration of charged groups in polymers have a key role in mucoadhesive interactions. A series of cationic poly(amino acid)s with different charge densities was designed to unravel the correlation between chemical structure and mucin-polymer interactions. Colloidal interactions between the mucin protein and synthetic polyaspartamides were tested by dynamic light scattering, zeta potential measurements and turbidimetric titration as a function of polymer-to-mucin mass ratio. The mucoadhesive interactions displayed a strongly non -linear change with polymer composition. The attractive interactions between mucin and the polyaspartamides with at least 50 % cationic groups caused increased light scattering of dispersions due to the aggregation of mucin particles upon their charge reversal. Interactions were further analysed in a thin mucin layer to model life-like situations using a quartz crystal microbalance (QCM) in flow mode. Results pointed out that the fully cationic polyaspartamide is not necessarily superior to derivatives with lower cationic group content. The maximum of adsorbed mass of polymers on mucin was experienced at medium cationic group contents. This emphasizes the relevance of cationic polyaspartamides as mucoadhesive excipients due to their multiple functionalities and the possibility of fine-tuning their interactions with mucin via straightforward chemical steps.},
	keywords = {POLYMERS; ADSORPTION; GEL; CHARGE; QUARTZ-CRYSTAL MICROBALANCE; mucoadhesion; QUARTZ CRYSTAL MICROBALANCE; polyelectrolyte; QCM; Poly(aspartic acid); GASTRIC MUCIN; MUCOADHESIVE INTERACTIONS},
	year = {2023},
	eissn = {1879-0003},
	orcid-numbers = {Ábrahám, Ágnes/0000-0003-2586-8650; Kiss, Éva/0000-0002-4757-4437; Varga, Zoltán/0000-0002-5741-2669; Szilágyi, András Ferenc/0000-0001-6898-1755}
}

@article{MTMT:34087548,
	title = {Standardization of extracellular vesicle concentration measurements by flow cytometry: the past, present, and future},
	url = {https://m2.mtmt.hu/api/publication/34087548},
	author = {Bettin, B.A. and Varga, Zoltán and Nieuwland, R. and van, der Pol E.},
	doi = {10.1016/j.jtha.2023.04.042},
	journal-iso = {J THROMB HAEMOST},
	journal = {JOURNAL OF THROMBOSIS AND HAEMOSTASIS},
	volume = {21},
	unique-id = {34087548},
	issn = {1538-7933},
	abstract = {Concentrations of extracellular vesicles (EVs) in body fluids are being explored as disease biomarkers. Most laboratories use flow cytometry to characterize single EVs at high throughput. A flow cytometer (FCM) detects light scattering and fluorescence intensities of EVs. However, detection of EVs by flow cytometry is complicated for 2 reasons. First, EVs are small and have weak light scattering and fluorescence signals compared to cells and are, therefore, hard to detect. Second, FCMs differ in sensitivity and provide data in arbitrary units, which complicates data interpretation. Due to the mentioned challenges, the measured concentration of EVs by flow cytometry is cumbersome to compare between FCMs and institutes. To improve comparability, standardization and development of traceable reference materials to calibrate all aspects of an FCM are needed, as are interlaboratory comparison studies. Within this article, we will provide an overview of the standardization of EV concentration measurements, including the current effort to introduce robust calibration of FCMs, thereby enabling comparable concentration measurements of EVs, which in turn can be used to establish clinically relevant reference ranges of EV concentrations in blood plasma and other body fluids. © 2023 The Authors},
	keywords = {Humans; PLASMA; PLASMA; STANDARDIZATION; human; CALIBRATION; CALIBRATION; CALIBRATION; standard; Flow Cytometry; Flow Cytometry; Flow Cytometry; Reference Standards; exosome; Extracellular vesicles; Extracellular vesicles; interlaboratory comparison study},
	year = {2023},
	eissn = {1538-7836},
	pages = {2032-2044},
	orcid-numbers = {Varga, Zoltán/0000-0002-5741-2669}
}

@article{MTMT:34044318,
	title = {Comparative Study of Molecular Mechanics Force Fields for β-Peptidic Foldamers: Folding and Self-Association},
	url = {https://m2.mtmt.hu/api/publication/34044318},
	author = {Wacha, András Ferenc and Varga, Zoltán and Beke-Somfai, Tamás},
	doi = {10.1021/acs.jcim.3c00175},
	journal-iso = {J CHEM INF MODEL},
	journal = {JOURNAL OF CHEMICAL INFORMATION AND MODELING},
	volume = {63},
	unique-id = {34044318},
	issn = {1549-9596},
	year = {2023},
	eissn = {1549-960X},
	pages = {3799-3813},
	orcid-numbers = {Wacha, András Ferenc/0000-0002-9609-0893; Varga, Zoltán/0000-0002-5741-2669}
}