TY - JOUR AU - Vályi, Péter AU - Wirth, Roland AU - Minárovits, János AU - Strang, Orsolya AU - Maróti, Gergely AU - Kovács, Kornél Lajos TI - The oral microbiome of a family including Papillon-Lefèvre-syndrome patients and clinically healthy members JF - BMC ORAL HEALTH J2 - BMC ORAL HEALTH VL - 24 PY - 2024 IS - 1 PG - 17 SN - 1472-6831 DO - 10.1186/s12903-024-03856-z UR - https://m2.mtmt.hu/api/publication/34558800 ID - 34558800 N1 - Department of Oral Diagnostics, Faculty of Dentistry, Semmelweis University, Szentkirályi u 47, Budapest, H1085, Hungary Department of Biotechnology, University of Szeged, Közép fasor 52, Szeged, H6726, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza L. krt 64, Szeged, H6720, Hungary Institute of Biophysics, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Institute of Plant Biology, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Export Date: 1 May 2024 Correspondence Address: Vályi, P.; Department of Oral Diagnostics, Szentkirályi u 47, Hungary; email: valyi.peter@dent.semmelweis-univ.hu Chemicals/CAS: dipeptidyl peptidase I, 9032-68-2 Funding details: European Regional Development Fund, ERDF, FK123899, GINOP-2.3.2-15-2016-00011, PD132145 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. Funding text 2: Open access funding provided by Semmelweis University. This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. AB - The oral microbiota composition of patients diagnosed with Papillon-Lefèvre-syndrome and treated for several years were compared to those existing in the oral cavity of the clinically healthy family members and a cohort of patients having various stages of chronic periodontitis. LA - English DB - MTMT ER - TY - JOUR AU - Tarjányi, Tamás AU - Bogár, Ferenc AU - Minárovits, János AU - Gajdács, Márió AU - Tóth, Zsolt TI - Interaction of biomolecules with anatase, rutile and amorphous TiO2 surfaces: A molecular dynamics study JF - PLOS ONE J2 - PLOS ONE VL - 18 PY - 2023 IS - 9 PG - 19 SN - 1932-6203 DO - 10.1371/journal.pone.0289467 UR - https://m2.mtmt.hu/api/publication/34127552 ID - 34127552 N1 - Funding Agency and Grant Number: University of Szeged [GINOP-2.3.2-15-2016-00011]; [6103] Funding text: This work was supported by the grant GINOP-2.3.2-15-2016-00011, in the initial phase and by the University of Szeged Open Access Fund (Grant number: 6103). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AB - The adhesion of biomolecules to dental and orthopedic implants is a fundamental step in the process of osseointegration. Short peptide motifs, such as RGD or KRSR, carried by extracellular matrix proteins or coated onto implant surfaces, accelerate cell adhesion and tissue formation. For this reason, understanding the binding mechanisms of adhesive peptides to oxidized surfaces of titanium implants is of paramount importance. We performed molecular dynamics simulations to compare the adhesion properties of 6 peptides, including the tripeptide RGD, its variants KGD and LGD, as well as the tetrapeptide KRSR, its variant LRSR and its truncated version RSR, on anatase, rutile, and amorphous titanium dioxide (TiO 2 ) surfaces. The migration of these molecules from the water phase to the surface was simulated in an aqueous environment. Based on these simulations, we calculated the residence time of each peptide bound to the three different TiO 2 structures. It was found that the presence of an N-terminal lysine or arginine amino acid residue resulted in more efficient surface binding. A pulling simulation was performed to detach the adhered molecules. The maximum pulling force and the binding energy were determined from the results of these simulations. The tri- and tetrapeptides had slightly greater adhesion affinity to the amorphous and anatase structure than to rutile in general, however specific surface and peptide binding characters could be detected. The binding energies obtained from our simulations allowed us to rank the adhesion strengths of the studied peptides. LA - English DB - MTMT ER - TY - CHAP AU - Minárovits, János ED - Takács, Mária TI - Daganatvírusok T2 - Orvosi virológia PB - Medicina Könyvkiadó Zrt. CY - Budapest SN - 9789632268484 PY - 2022 SP - 502 EP - 506 PG - 5 UR - https://m2.mtmt.hu/api/publication/33562016 ID - 33562016 LA - Hungarian DB - MTMT ER - TY - CONF AU - Áy, Éva AU - Adravecz, Lilla AU - Pocskay, Ágnes AU - Müller, Viktor AU - Lakatos, Botond AU - Szlávik, János AU - Pék, Tamás AU - Marschalkó, Márta AU - Tóth, Béla AU - Kárpáti, Sarolta AU - Mezei, Mária AU - Minárovits, János TI - Átvitt HIV gyógyszer-rezisztencia jelentősége a klinikai gyakorlatban T2 - Magyar Infektológiai és Klinikai Mikrobiológiai Társaság 49. Kongresszusa PY - 2022 SP - 62 EP - 62 PG - 1 UR - https://m2.mtmt.hu/api/publication/33162598 ID - 33162598 LA - Hungarian DB - MTMT ER - TY - CONF AU - Tarjányi, Tamás AU - Bogár, Ferenc AU - Gajdács, Márió AU - Minárovits, János AU - Tóth, Zsolt ED - Baráth, Zoltán Lajos ED - Stájer, Anette ED - Madléna, Melinda ED - Matusovits, Danica ED - Kárpáti, Krisztina ED - Gajdács, Márió TI - Osszeointegráció molekuláris megközelítésben: kristályos és amorf titán-oxid felületekkel kölcsönható biomolekulák T2 - Szegedi Fogorvosnapok 2022. Szegedi Fogorvos Találkozó és Tudományos Konferencia, Magyar Gyermekfogászati és Fogszabályozási Társaság IX. Tóth Pál Vándorgyűlés PB - Szegedi Tudományegyetem Fogorvostudományi Kar C1 - Szeged SN - 9786150161211 PY - 2022 SP - 25 EP - 25 PG - 1 UR - https://m2.mtmt.hu/api/publication/33118459 ID - 33118459 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Takács, Mária AU - Minárovits, János ED - Takács, Mária TI - Coronaviridae T2 - Orvosi virológia PB - Medicina Könyvkiadó Zrt. CY - Budapest SN - 9789632268484 PY - 2022 SP - 154 EP - 162 PG - 9 UR - https://m2.mtmt.hu/api/publication/33094830 ID - 33094830 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Minárovits, János TI - Megemlékezés Földes István mikrobiológus, az orvostudomány doktora születésének centenáriumán = A Tribute to István Földes, DSc, Microbiologist on the Centenary of his Birth JF - MAGYAR TUDOMÁNY J2 - MAGYAR TUDOMÁNY VL - 183 PY - 2022 IS - 2 SP - 247 EP - 249 PG - 3 SN - 0025-0325 DO - 10.1556/2065.183.2022.2.12 UR - https://m2.mtmt.hu/api/publication/32788115 ID - 32788115 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Fülöp, Ádám AU - Torma, Gábor AU - Moldován, Norbert AU - Szenthe, Kálmán AU - Bánáti, Ferenc AU - Almsarrhad, Islam AU - Csabai, Zsolt AU - Tombácz, Dóra AU - Minárovits, János AU - Boldogkői, Zsolt TI - Integrative profiling of Epstein–Barr virus transcriptome using a multiplatform approach JF - VIROLOGY JOURNAL J2 - VIROL J VL - 19 PY - 2022 IS - 1 PG - 17 SN - 1743-422X DO - 10.1186/s12985-021-01734-6 UR - https://m2.mtmt.hu/api/publication/32581214 ID - 32581214 AB - Background Epstein-Barr virus (EBV) is an important human pathogenic gammaherpesvirus with carcinogenic potential. The EBV transcriptome has previously been analyzed using both Illumina-based short read-sequencing and Pacific Biosciences RS II-based long-read sequencing technologies. Since the various sequencing methods have distinct strengths and limitations, the use of multiplatform approaches have proven to be valuable. The aim of this study is to provide a more complete picture on the transcriptomic architecture of EBV. Methods In this work, we apply the Oxford Nanopore Technologies MinION (long-read sequencing) platform for the generation of novel transcriptomic data, and integrate these with other's data generated by another LRS approach, Pacific BioSciences RSII sequencing and Illumina CAGE-Seq and Poly(A)-Seq approaches. Both amplified and non-amplified cDNA sequencings were applied for the generation of sequencing reads, including both oligo-d(T) and random oligonucleotide-primed reverse transcription. EBV transcripts are identified and annotated using the LoRTIA software suite developed in our laboratory. Results This study detected novel genes embedded into longer host genes containing 5 '-truncated in-frame open reading frames, which potentially encode N-terminally truncated proteins. We also detected a number of novel non-coding RNAs and transcript length isoforms encoded by the same genes but differing in their start and/or end sites. This study also reports the discovery of novel splice isoforms, many of which may represent altered coding potential, and of novel replication-origin-associated transcripts. Additionally, novel mono- and multigenic transcripts were identified. An intricate meshwork of transcriptional overlaps was revealed. Conclusions An integrative approach applying multi-technique sequencing technologies is suitable for reliable identification of complex transcriptomes because each techniques has different advantages and limitations, and the they can be used for the validation of the results obtained by a particular approach. LA - English DB - MTMT ER - TY - JOUR AU - Wirth, Roland AU - Maróti, Gergely AU - Lipták, Lídia AU - Mester, Mónika Klára AU - Al Ayoubi, Alaa AU - Pap, Bernadett AU - Madléna, Melinda AU - Minárovits, János AU - Kovács, Kornél Lajos TI - Microbiomes in supragingival biofilms and saliva of adolescents with gingivitis and gingival health JF - ORAL DISEASES J2 - ORAL DIS VL - 28 PY - 2022 IS - 7 SP - 2000 EP - 2014 PG - 15 SN - 1354-523X DO - 10.1111/odi.13883 UR - https://m2.mtmt.hu/api/publication/32020395 ID - 32020395 N1 - Department of Biotechnology, University of Szeged, Szeged, Hungary Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Orthodontics and Pediatric Dentistry, Faculty of Dentistry, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Export Date: 20 October 2021 CODEN: ORDIF Correspondence Address: Kovács, K.L.; Department of Biotechnology, Hungary; email: kovacs.kornel@bio.u-szeged.hu Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Regional Development Fund, ERDF, FK123899, GINOP‐2.3.2‐15‐2016‐00011, PD132145 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH Funding details: National Research, Development and Innovation Office Funding text 1: This research was funded by the European Regional Development Fund to a project led by JM grant GINOP‐2.3.2‐15‐2016‐00011. Additional projects GINOP‐2.2.1‐15‐2017‐00081, GINOP‐2.2.1‐15‐2017‐00033, and EFOP‐3.6.2‐16‐2017‐00010 also contributed to this work. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. This work was also supported by the János Bolyai Research Scholarship (for GM) of the Hungarian Academy of Sciences. The authors thank the funding agencies for their support. We are indebted to Dr. Márió Gajdács and the unknown Reviewers for critical reading of the manuscript. AB - Background Important alterations exist in the microbiomes of supragingival biofilm and saliva samples from adolescent patients developing induced or spontaneous gingivitis relative to healthy controls. These and the relationships to dental health are not fully understood yet. Subjects and Methods Supragingival biofilm samples (n = 36) were collected from the teeth of 9 adolescents with gingivitis induced by orthodontic appliances, as well as dental plaques (n = 40) from 10 adolescents with spontaneous gingivitis, in addition to similar samples (n = 36) from 9 healthy controls. The bacterial metagenomes were analyzed by 16S rRNA gene amplicon sequencing. Salivary microbiomes of the same persons were characterized by shotgun metagenome sequencing. The data sets were examined using advanced bioinformatics workflows and two reference databases. Results The composition and diversity of bacterial communities did not differ extensively among the three study groups. Nevertheless, the relative abundances of the genera Fusobacterium, Akkermansia, Treponema, and Campylobacter were prominently higher in gingivitis patients versus controls. In contrast, the genera Lautropia, Kingella, Neisseria, Actinomyces, and Rothia were significantly more abundant in controls than in either of the two gingivitis groups. Conclusions The abundance pattern of certain taxa rather than individual strains shows characteristic features of potential diagnostic value. Stringent bioinformatics treatment of the sequencing data is mandatory to avoid unintentional misinterpretations. LA - English DB - MTMT ER - TY - CHAP AU - Chofong, G.N. AU - Minárovits, János AU - Richert-Pöggeler, K.R. ED - Gaur, Rajarshi Kumar ED - Khurana, S.M. Paul ED - Sharma, Pradeep ED - Hohn, Thomas TI - Virus latency: Heterogeneity of host-virus interaction in shaping the virosphere T2 - Plant Virus-Host Interaction PB - Academic Press CY - London SN - 9780128216293 PY - 2021 SP - 111 EP - 137 PG - 27 DO - 10.1016/B978-0-12-821629-3.00016-6 UR - https://m2.mtmt.hu/api/publication/32776471 ID - 32776471 N1 - Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Messeweg 11-12, Braunschweig, D-38104, Germany University of Szeged, Faculty of Dentistry, Department of Oral Biology and Experimental Dental Research, Tisza Lajos krt. 64, Szeged, H-6720, Hungary Cited By :1 Export Date: 7 April 2022 AB - Viruses and viroids that are not causing symptoms on their initial host plant are described by the terms “latent”, “cryptic” or “symptomless” and represent 6% and 2% of the classified plant viruses and viroids, respectively. Additionally, endogenous plant pararetroviruses that reside in their host genome are often dormant and therefore asymptomatic. Improved sequencing and diagnostic technology demonstrated that viral sequences are ubiquitously present in the biosphere even if the signs or symptoms of infection are not obvious. Accordingly, the number of latent plant viruses listed today does not reflect the true amount of latent viruses existing in plants. Biodiversity within the virosphere comprises virus-host, virus-vector, virus-virus and virus-viroid interactions. Invasion of new host plants, climatic changes and changes in plant production and distribution can have a major impact on symptomatic outbreaks of otherwise latent viruses which seem incapacitated but remain potentially undefeated. Here we describe for selected ornamentals how these changes can induce latent viruses and report on possible underlying biochemical mechanisms. Comparison of virus latency in plant and animal hosts indicates that epigenetic modifications are an important factor for regulation in both systems. © 2021 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER -