TY - JOUR AU - Kiss, Tamás AU - Mir, Mohd Yaqub AU - Stefancsik, Gergely AU - Ganbat, Gantulga AU - Askarova, Aruzhan AU - Monostori, Éva AU - Dulka, Karolina AU - Szebeni, Gábor AU - Nyúl-Tóth, Ádám AU - Csiszar, Anna AU - Légrádi, Ádám TI - Galectin-1 as a marker for microglia activation in the aging brain JF - BRAIN RESEARCH J2 - BRAIN RES VL - 1818 PY - 2023 PG - 13 SN - 0006-8993 DO - 10.1016/j.brainres.2023.148517 UR - https://m2.mtmt.hu/api/publication/34093747 ID - 34093747 N1 - Funding Agency and Grant Number: American Heart Association [GINOP-2.3.2-15-2016-00034, 142877 FK22]; National Research, Development, and Innovation Office (NKFI) , Hungary [AHA834339]; Ministry for Innovation and Technology from the National Research, Development and Innovation Fund; American Heart Association; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; [EFOP-3.6.1-16-2016-00008]; [2020-1.1.6-JOVO-2021-00003]; [UNKP-22-5-SZTE-535]; [BO/00582/22/8] Funding text: This work was supported by a grant from EFOP-3.6.1-16-2016-00008 and GINOP-2.3.2-15-2016-00034 grants. ANyT was supported by American Heart Association (AHA834339) . (The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript) . This research was funded by the 2020-1.1.6-JOVO-2021-00003 and 142877 FK22, grant from the National Research, Development, and Innovation Office (NKFI) , Hungary. This work was supported by the UNKP-22-5-SZTE-535 New National Excellence Program (GJS) of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. This work was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences BO/00582/22/8 (GJS) . AB - Microglia cells, the immune cells residing in the brain, express immune regulatory molecules that have a central role in the manifestation of age-related brain characteristics. Our hypothesis suggests that galectin-1, an anti-inflammatory member of the beta-galactoside-binding lectin family, regulates microglia and neuroinflammation in the aging brain. Through our in-silico analysis, we discovered a subcluster of microglia in the aged mouse brain that exhibited increased expression of galectin-1 mRNA. In our Western blotting experiments, we observed a decrease in galectin-1 protein content in our rat primary cortical cultures over time. Additionally, we found that the presence of lipopolysaccharide, an immune activator, significantly increased the expression of galectin-1 protein in microglial cells. Utilizing flow cytometry, we determined that a portion of the galectin-1 protein was localized on the surface of the microglial cells. As cultivation time increased, we observed a decrease in the expression of activation-coupled molecules in microglial cells, indicating cellular exhaustion. In our mixed rat primary cortical cell cultures, we noted a transition of amoeboid microglial cells labeled with OX42(CD11b/c) to a ramified, branched phenotype during extended cultivation, accompanied by a complete disappearance of galectin-1 expression. By analyzing the transcriptome of a distinct microglial subpopulation in an animal model of aging, we established a correlation between chronological aging and galectin-1 expression. Furthermore, our in vitro study demonstrated that galectin-1 expression is associated with the functional activation state of microglial cells exhibiting specific amoeboid morphological characteristics. Based on our findings, we identify galectin-1 as a marker for microglia activation in the context of aging. LA - English DB - MTMT ER - TY - CONF AU - Kiss, Tamás AU - Mir, Mohd Yaqub AU - Stefancsik, Gergely AU - Gantulga, Ganbat AU - Aruzhan, Askarova AU - Monostori, Éva AU - István, Pesti AU - Karolina, Dulka AU - Gábor, Szebeni J. AU - Nyúl-Tóth, Ádám AU - Csiszar, Anna AU - Eszter, Farkas AU - Légrádi, Ádám TI - Galectin-1 as a marker for microglia activation in the aging brain T2 - Joint Neuroscience Meeting of the Hungarian Neuroscience Society (MITT) & the Austrian Neuroscience Association (ANA) PY - 2023 SP - 74 EP - 74 PG - 1 UR - https://m2.mtmt.hu/api/publication/33719598 ID - 33719598 N1 - Poszter LA - English DB - MTMT ER - TY - JOUR AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Imre, Norbert AU - Nath Bhaumik, Kaushik AU - Tököli, Attila AU - Füzesi, Tamás AU - Hollandi, Réka AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Deli, Mária Anna AU - Martinek, Tamás TI - α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 14 PY - 2022 IS - 3 PG - 11 SN - 1999-4923 DO - 10.3390/pharmaceutics14030580 UR - https://m2.mtmt.hu/api/publication/32733913 ID - 32733913 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.2.1-15-2016-00007]; Hungarian Ministry of Innovation and Technology [TUDFO/47138-1/2019-ITM]; Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG); Hungarian Academy of Sciences LENDULET-FoldamerHungarian Academy of Sciences; NKFINational Research, Development & Innovation Office (NRDIO) - Hungary [K134754]; Finnish TEKES FiDiPro Fellow Grant [40294/13]; Hungarian Academy of Sciences LENDULET-BiomagHungarian Academy of Sciences; Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund [TKP2021-EGA-32] Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and the Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer, and NKFI K134754. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, and the Hungarian Academy of Sciences LENDULET-Biomag. Support by the Ministry of Innovation and Technology of Hungary through the National Research, Development and Innovation Fund (TKP2021-EGA-32) is acknowledged. AB - Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short alpha/beta-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform. LA - English DB - MTMT ER - TY - JOUR AU - Hetényi, Anasztázia AU - Imre, Norbert AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Fehérje méretű molekulák humán sejtekbe juttatása lipid-raft mediált endocitózissal JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - 45 PY - 2021 IS - 4 SP - 67 EP - 83 PG - 17 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32570862 ID - 32570862 N1 - Nincs jelölve levelező szerzőség a közleményen. (BÉ SZTE admin5) LA - Hungarian DB - MTMT ER - TY - JOUR AU - Matula, Zsolt AU - Mikala, Gábor AU - Lukácsi, Szilvia Zsófia AU - Matkó, János AU - Kovács, Tamás AU - Monostori, Éva AU - Uher, Ferenc AU - Vályi-Nagy, István TI - Stromal Cells Serve Drug Resistance for Multiple Myeloma via Mitochondrial Transfer: A Study on Primary Myeloma and Stromal Cells JF - CANCERS J2 - CANCERS VL - 13 PY - 2021 IS - 14 PG - 24 SN - 2072-6694 DO - 10.3390/cancers13143461 UR - https://m2.mtmt.hu/api/publication/32107638 ID - 32107638 LA - English DB - MTMT ER - TY - JOUR AU - Tököli, Attila AU - Mag, Beáta Zsófia AU - Bartus, Éva AU - Wéber, Edit AU - Szakonyi, Gerda AU - Simon, Márton AU - Czibula, Ágnes AU - Monostori, Éva AU - Nyitray, László AU - Martinek, Tamás TI - Proteomimetic surface fragments distinguish targets by function JF - CHEMICAL SCIENCE J2 - CHEM SCI VL - 11 PY - 2020 IS - 38 SP - 10390 EP - 10398 PG - 9 SN - 2041-6520 DO - 10.1039/d0sc03525d UR - https://m2.mtmt.hu/api/publication/31598466 ID - 31598466 N1 - Department of Medical Chemistry, University of Szeged, Dóm tér 8, Szeged, H6720, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Dóm tér 8, Szeged, H6720, Hungary Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4., Szeged, H6720, Hungary Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, H1077, Hungary Lymphocyte Signal Transduction Laboratory, Institute of Genetics, Biological Research Centre, Temesvári krt. 62, H6726 Szeged, Hungary Cited By :3 Export Date: 12 March 2024 CODEN: CSHCC Correspondence Address: Nyitray, L.; Department of Biochemistry, Pázmány Péter sétány 1/C, Hungary; email: nyitray@elte.hu LA - English DB - MTMT ER - TY - JOUR AU - Kriston-Pál, Éva AU - Haracska, Lajos AU - Cooper, P. AU - Kiss-Tóth, E. AU - Szukacsov, Valéria AU - Monostori, Éva TI - A Regenerative Approach to Canine Osteoarthritis Using Allogeneic, Adipose-Derived Mesenchymal Stem Cells. Safety Results of a Long-Term Follow-Up JF - FRONTIERS IN VETERINARY SCIENCE J2 - FRONT VET SCI VL - 7 PY - 2020 SN - 2297-1769 DO - 10.3389/fvets.2020.00510 UR - https://m2.mtmt.hu/api/publication/31598021 ID - 31598021 N1 - Stem CellX Limited, Szeged, Hungary Institute of Genetics, Biological Research Centre, Szeged, Hungary Assentra Limited, Chelmsford, United Kingdom University of Sheffield, Department of Infection, Immunity Cardiovascular Disease, Sheffield, United Kingdom Export Date: 11 September 2020 Correspondence Address: Monostori, É.; Institute of Genetics, Biological Research CentreHungary; email: monostori.eva@brc.hu Stem CellX Limited, Szeged, Hungary Institute of Genetics, Biological Research Centre, Szeged, Hungary Assentra Limited, Chelmsford, United Kingdom University of Sheffield, Department of Infection, Immunity Cardiovascular Disease, Sheffield, United Kingdom Export Date: 6 October 2020 Correspondence Address: Monostori, É.; Institute of Genetics, Biological Research CentreHungary; email: monostori.eva@brc.hu Stem CellX Limited, Szeged, Hungary Institute of Genetics, Biological Research Centre, Szeged, Hungary Assentra Limited, Chelmsford, United Kingdom University of Sheffield, Department of Infection, Immunity Cardiovascular Disease, Sheffield, United Kingdom Export Date: 16 February 2021 Correspondence Address: Monostori, É.; Institute of Genetics, Hungary; email: monostori.eva@brc.hu Stem CellX Limited, Szeged, Hungary Institute of Genetics, Biological Research Centre, Szeged, Hungary Assentra Limited, Chelmsford, United Kingdom University of Sheffield, Department of Infection, Immunity Cardiovascular Disease, Sheffield, United Kingdom Export Date: 17 February 2021 Correspondence Address: Monostori, É.; Institute of Genetics, Hungary; email: monostori.eva@brc.hu Stem CellX Limited, Szeged, Hungary Institute of Genetics, Biological Research Centre, Szeged, Hungary Assentra Limited, Chelmsford, United Kingdom University of Sheffield, Department of Infection, Immunity Cardiovascular Disease, Sheffield, United Kingdom Export Date: 31 August 2021 Correspondence Address: Monostori, É.; Institute of Genetics, Hungary; email: monostori.eva@brc.hu AB - Mesenchymal stem cells (MSC) are emerging as an effective therapeutic tool in treating canine osteoarthritis (OA). In this report, we focused on the questions of whether MSC transplantation has long-term beneficial effects for the improvement in motion and also evaluated the safety of MSC injection. Visceral adipose tissue, a surgical waste obtained during routine ovariectomy served as a source of allogeneic MSCs and used to treat OA. Altogether, fifty-eight dogs were transplanted in the study suffering from OA in the elbow (42 animals), hip (5), knee (8), ankle (2), and hock (1). The effect of MSC transplantation was evaluated by the degree of lameness at a 4-5-years follow-up period based on the owners' subjective observations. The results showed that 83% of the OA patients improved or retained improvement in lameness. Clinical safety of the treatment was assessed by evaluating the coincidence of tumors or other diseases and other adverse reactions (such as local inflammation) after MSC cell therapy. Two incidences of local inflammation for <1 week at the site of injection were reported. No other adverse reactions were detected post-treatment. Sixteen dogs died during the study, 4 due to cancer and 12 due to other diseases, diagnosed by veterinarians. Overall, our survey suggests that MSC transplantation has long-term beneficial effects in reducing lameness. Moreover, no enrichment in a specific cause of death was observed in the transplanted animals, compared to reported literature. Our data suggest that MSC treatment could be an effective and safe long-term therapy for canine OA. © Copyright © 2020 Kriston-Pál, Haracska, Cooper, Kiss-Tóth, Szukacsov and Monostori. LA - English DB - MTMT ER - TY - JOUR AU - Imre, Norbert AU - Hetényi, Anasztázia AU - Szabó, Enikő AU - Bodnár, Brigitta AU - Szkalisity, Ábel AU - Gróf, Ilona AU - Bocsik, Alexandra AU - Deli, Mária Anna AU - Horváth, Péter AU - Czibula, Ágnes AU - Monostori, Éva AU - Martinek, Tamás TI - Routing Nanomolar Protein Cargoes to Lipid Raft‐Mediated/Caveolar Endocytosis through a Ganglioside GM1‐Specific Recognition Tag JF - ADVANCED SCIENCE J2 - ADV SCI VL - 7 PY - 2020 IS - 4 PG - 10 SN - 2198-3844 DO - 10.1002/advs.201902621 UR - https://m2.mtmt.hu/api/publication/31126947 ID - 31126947 N1 - Funding text: This research was funded by the National Research, Development and Innovation Office of Hungary, grant number GINOP-2.2.1-15-2016-00007, the Hungarian Ministry of Innovation and Technology, TUDFO/47138-1/2019-ITM, and Hungarian National Brain Research Program (MTA-SE-NAP B-BIOMAG). T.A.M. acknowledges support from the Hungarian Academy of Sciences LENDULET-Foldamer. P.H. acknowledges support from the Finnish TEKES FiDiPro Fellow Grant 40294/13, the Hungarian Academy of Sciences LENDULET-Biomag. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Hornung, Ákos AU - Monostori, Éva AU - Bocskai, Márta AU - Czibula, Ágnes AU - Kovács, László TI - Altered Cell Surface N-Glycosylation of Resting and Activated T Cells in Systemic Lupus Erythematosus JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 20 PY - 2019 IS - 18 PG - 14 SN - 1661-6596 DO - 10.3390/ijms20184455 UR - https://m2.mtmt.hu/api/publication/30802849 ID - 30802849 N1 - Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Rheumatology and Immunology, Faculty of Medicine, University of Szeged, Szeged, 6725, Hungary Export Date: 29 November 2019 Correspondence Address: Czibula, Á.; Institute of Genetics, Biological Research Centre of the Hungarian Academy of SciencesHungary; email: czibula.agnes@brc.hu Chemicals/CAS: galectin 1, 258495-34-0; sialidase, 9001-67-6; sialyltransferase, 9075-81-4 Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, 6726, Hungary Department of Rheumatology and Immunology, Faculty of Medicine, University of Szeged, Szeged, 6725, Hungary Export Date: 2 December 2019 Correspondence Address: Czibula, Á.; Institute of Genetics, Biological Research Centre of the Hungarian Academy of SciencesHungary; email: czibula.agnes@brc.hu Chemicals/CAS: galectin 1, 258495-34-0; sialidase, 9001-67-6; sialyltransferase, 9075-81-4 LA - English DB - MTMT ER - TY - JOUR AU - Hornung, Ákos AU - Monostori, Éva AU - Kovács, László TI - Systemic lupus erythematosus in the light of the regulatory effects of galectin-1 on T-cell function JF - LUPUS J2 - LUPUS VL - 26 PY - 2017 IS - 4 SP - 339 EP - 347 PG - 9 SN - 0961-2033 DO - 10.1177/0961203316686846 UR - https://m2.mtmt.hu/api/publication/3190069 ID - 3190069 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 18 September 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 29 November 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Szeged, 6725, Hungary Cited By :1 Export Date: 2 December 2019 CODEN: LUPUE Correspondence Address: Kovács, L.; Department of Rheumatology and Immunology, University of Szeged, Faculty of Medicine, Albert Szent-Györgyi Health Centre, Ká lvária sgt. 57, Hungary; email: kovacs.laszlo@med.u-szeged.hu Chemicals/CAS: galectin 1, 258495-34-0; ganglioside GM1, 37758-47-7; phosphatidylinositol 3 kinase, 115926-52-8; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; protein kinase Lck, 114051-78-4; protein kinase Syk, 138674-26-7; protein kinase ZAP 70, 148047-34-1; Galectin 1; LGALS1 protein, human AB - Galectin-1 is an endogenous immunoregulatory lectin-type protein. Its most important effects are the inhibition of the differentiation and cytokine production of Th1 and Th17 cells, and the induction of apoptosis of activated T-cells. Galectin-1 has been identified as a key molecule in antitumor immune surveillance, and data are accumulating about the pathogenic role of its deficiency, and the beneficial effects of its administration in various autoimmune disease models. Initial animal and human studies strongly suggest deficiencies in both galectin-1 production and responsiveness in systemic lupus erythematosus (SLE) T-cells. Since lupus features widespread abnormalities in T-cell activation, differentiation and viability, in this review the authors wished to highlight potential points in T-cell signalling processes that may be influenced by galectin-1. These points include GM-1 ganglioside-mediated lipid raft aggregation, early activation signalling steps involving p56Lck, the exchange of the CD3 zeta-ZAP-70 to the FcRgamma-Syk pathway, defective mitogen-activated protein kinase pathway activation, impaired regulatory T-cell function, the failure to suppress the activity of interleukin 17 (IL-17) producing T-cells, and decreased suppression of the PI3K-mTOR pathway by phosphatase and tensin homolog (PTEN). These findings place galectin-1 into the group of potential pathogenic molecules in SLE. LA - English DB - MTMT ER -