TY - JOUR AU - Szabad, János AU - Zádori, János TI - Az aneuploidia eredete. A kevesebb és a több sem jó TS - A kevesebb és a több sem jó JF - TERMÉSZET VILÁGA J2 - TERMÉSZET VILÁGA VL - 153 PY - 2022 IS - 12 SP - 560 EP - 567 PG - 8 SN - 0040-3717 UR - https://m2.mtmt.hu/api/publication/33648936 ID - 33648936 N1 - Nincs jelölve levelező szerzőség a közleményen. (TP SZTE admin5) LA - Hungarian DB - MTMT ER - TY - JOUR AU - Zádori, János AU - Szabad, János TI - A kevesebb és a több sem jó. Az aneuploidia eredete és következményei TS - Az aneuploidia eredete és következményei JF - TERMÉSZET VILÁGA J2 - TERMÉSZET VILÁGA VL - 153 PY - 2022 IS - 11 SP - 512 EP - 517 PG - 6 SN - 0040-3717 UR - https://m2.mtmt.hu/api/publication/33648918 ID - 33648918 N1 - Nincs jelölve levelező szerzőség a közleményen. (TP SZTE admin5) LA - Hungarian DB - MTMT ER - TY - BOOK AU - Szabad, János TI - Genetic Mosaicism PB - Cambridge Scholars Publishing CY - Cambridge PY - 2021 SP - 160 SN - 9781527570580 UR - https://m2.mtmt.hu/api/publication/32661336 ID - 32661336 AB - The presence of green and yellow ornamental plants around us, dark spots on our skin, people with brown and blue mosaic irises, and white-spotted dogs and horses are all well-known phenomena of life, and are recognisable genetic mosaics. Although such genetics mosaics live with us (and, in fact, we are all a genetic mosaics), little is known about the genetic bases of their origin. This book provides a general overview of the mechanisms that lead to the formation of different types of mosaics, listing an ample collection of examples to illustrate the impact of the genetic mosaics on our life. The book will appeal to the reader interested in understanding the relationship between genetic events and mosaicism, especially undergraduate and graduate students and medical doctors, as well as experts engaged in horticulture and animal breeding. LA - English DB - MTMT ER - TY - JOUR AU - Szabad, János AU - Peng, Jing AU - Kubli, Eric TI - Control of Mating Plug Expelling and Sperm Storage in Drosophila: A Gynandromorph- and Mutation-Based Dissection JF - BIOLOGIA FUTURA J2 - BIOL FUTURA VL - 70 PY - 2019 IS - 4 SP - 301 EP - 311 PG - 11 SN - 2676-8615 DO - 10.1556/019.70.2019.34 UR - https://m2.mtmt.hu/api/publication/31295471 ID - 31295471 AB - Introduction: In this study, we analyzed gynandromorphs with female terminalia, to dissect mating-related female behaviors in Drosophila. Materials and methods: We used gynandromorphs, experimentally modified wild-type (Oregon-R) females, and mutant females that lacked different components of the female reproductive apparatus. Results: Many of the gynandromorphs mated but did not expel the mating plug (MP). Some of these – with thousands of sperm in the uterus – failed to take up sperm into the storage organs. There were gynandromorphs that stored plenty of sperm but failed to release them to fertilize eggs. Expelling the MP, sperm uptake into the storage organs, and the release of stored sperm along egg production are separate steps occurring during Drosophila female fertility. Cuticle landmarks of the gynandromorphs revealed that while the nerve foci that control MP expelling and also those that control sperm uptake reside in the abdominal, the sperm release foci derive from the thoracic region of the blastoderm. Discussion and conclusion: The gynandromorph study is confirmed by analyses of (a) mutations that cause female sterility: Fs(3)Avar (preventing egg deposition), Tm2gs (removing germline cells), and iab-4DB (eliminating gonad formation) and (b) by experimentally manipulated wild-type females: decapitated or cut through ventral nerve cord. LA - English DB - MTMT ER - TY - BOOK AU - Szabad, János TI - Genetikai mozaikok PB - JATEPress CY - Szeged PY - 2019 SP - 126 SN - 9789633154076 UR - https://m2.mtmt.hu/api/publication/31207173 ID - 31207173 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szabad, János TI - Mi van beleírva kromoszómáinkba? JF - TERMÉSZET VILÁGA J2 - TERMÉSZET VILÁGA VL - 148 PY - 2017 IS - 6 SP - 242 EP - 246 PG - 5 SN - 0040-3717 UR - https://m2.mtmt.hu/api/publication/3243692 ID - 3243692 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szabad, János TI - Belső óra, napi ritmus JF - TERMÉSZET VILÁGA J2 - TERMÉSZET VILÁGA VL - 146 PY - 2015 IS - 2. különszám SP - 72 EP - 77 PG - 6 SN - 0040-3717 UR - https://m2.mtmt.hu/api/publication/3006363 ID - 3006363 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Laukó, Zoltán AU - Szabad, János TI - Élet a cserépodúban JF - TERMÉSZET VILÁGA J2 - TERMÉSZET VILÁGA VL - 146 PY - 2015 IS - 5 SP - 209 EP - 210 PG - 2 SN - 0040-3717 UR - https://m2.mtmt.hu/api/publication/2930170 ID - 2930170 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Handke, B AU - Szabad, János AU - Lidsky, PV AU - Hafen, E AU - Lehner, CF TI - Towards long term cultivation of Drosophila wing imaginal discs in vitro JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 9 PG - 16 SN - 1932-6203 DO - 10.1371/journal.pone.0107333 UR - https://m2.mtmt.hu/api/publication/2984167 ID - 2984167 AB - The wing imaginal disc of Drosophila melanogaster is a prominent experimental system for research on control of cell growth, proliferation and death, as well as on pattern formation and morphogenesis during organogenesis. The precise genetic methodology applicable in this system has facilitated conceptual advances of fundamental importance for developmental biology. Experimental accessibility and versatility would gain further if long term development of wing imaginal discs could be studied also in vitro. For example, culture systems would allow live imaging with maximal temporal and spatial resolution. However, as clearly demonstrated here, standard culture methods result in a rapid cell proliferation arrest within hours of cultivation of dissected wing imaginal discs. Analysis with established markers for cells in S- and M phase, as well as with RGB cell cycle tracker, a novel reporter transgene, revealed that in vitro cultivation interferes with cell cycle progression throughout interphase and not just exclusively during G1. Moreover, quantification of EGFP expression from an inducible transgene revealed rapid adverse effects of disc culture on basic cellular functions beyond cell cycle progression. Disc transplantation experiments confirmed that these detrimental consequences do not reflect fatal damage of imaginal discs during isolation, arguing clearly for a medium insufficiency. Alternative culture media were evaluated, including hemolymph, which surrounds imaginal discs during growth in situ. But isolated larval hemolymph was found to be even less adequate than current culture media, presumably as a result of conversion processes during hemolymph isolation or disc culture. The significance of prominent growth-regulating pathways during disc culture was analyzed, as well as effects of insulin and disc co-culture with larval tissues as potential sources of endocrine factors. Based on our analyses, we developed a culture protocol that prolongs cell proliferation in cultured discs. LA - English DB - MTMT ER - TY - JOUR AU - Soós, István AU - Szabad, János TI - Assaying benzene, a parguet varnish and a synthetic thinner with respect to induction of in vivo chromosome loss in wing primordial cells of Drosophila JF - MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS J2 - MUTAT RES-GEN TOX EN VL - 763 PY - 2014 SP - 18 EP - 22 PG - 5 SN - 1383-5718 DO - 10.1016/j.mrgentox.2013.11.007 UR - https://m2.mtmt.hu/api/publication/2392076 ID - 2392076 LA - English DB - MTMT ER -