@article{MTMT:34913707,
	title = {Novel Transmission Electron Microscopic Method to Quantify Protein Clustering on the Cell Surface},
	url = {https://m2.mtmt.hu/api/publication/34913707},
	author = {Kormos, József and Daróczi, Lajos and Szöllősi, János and Mátyus, László and Jenei, Attila},
	doi = {10.1002/cpz1.1045},
	journal-iso = {CURR PROT},
	journal = {CURRENT PROTOCOLS},
	volume = {4},
	unique-id = {34913707},
	issn = {2691-1299},
	abstract = {The cell surface distribution patterns (clustering) of membrane proteins have been widely investigated in cell biology. Here we describe a novel transmission electron microscopic (TEM) protocol designed to improve the quality of information obtained about the protein distribution patterns detected. This novel method makes it possible to study the clustering of all transmembrane proteins on one half of the cytoplasmic membrane of a whole cell. To achieve better imaging, we combine various methods, including critical‐point drying, fixation of gold beads with a carbon layer, and a newly developed chemical thinning method. In addition, in our image‐processing algorithm, we implemented pair correlation and pair cross‐correlation functions, providing more details and better quantitative accuracy in characterizing the size and numbers of possible protein clusters. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.},
	year = {2024},
	eissn = {2691-1299}
}

@article{MTMT:34129378,
	title = {HLA DQ protein changes the cell surface distribution pattern of HLA proteins as monitored by Förster resonance energy transfer and high-resolution electron microscopy},
	url = {https://m2.mtmt.hu/api/publication/34129378},
	author = {Kormos, József and Veres, Adrienn J. and Imre, László and Mátyus, László and Benkő, Szilvia and Szöllősi, János and Jenei, Attila},
	doi = {10.1002/cyto.a.24787},
	journal-iso = {CYTOM PART A},
	journal = {CYTOMETRY PART A},
	volume = {103},
	unique-id = {34129378},
	issn = {1552-4922},
	abstract = {Peptide presentation by MHC class I and MHC class II molecules plays important roles in the regulation of the immune response. One factor in these displays is the density of antigen, which must exceed a critical threshold for the effective activation of T cells. Nonrandom distribution of MHC class I and class II has already been detected at the nanometer level and at higher hierarchical levels. It is not clear how the absence and reappearance of some protein molecules can influence the nonrandom distribution. Therefore, we performed experiments on HLA II‐deficient bare lymphocyte syndrome (BLS1) cells: we created a stable transfected cell line, tDQ6‐BLS‐1, and were able to detect the effect of the appearance of HLA‐DQ6 molecules on the homo and heteroassociation of different cell surface molecules by comparing Förster resonance energy transfer (FRET) efficiency on transfected cells to that on nontransfected BLS‐1 and JY human B‐cell lines. Our FRET results show a decrease in homoassociation FRET between HLA I chains in HLA‐DQ6‐transfected tDQ6‐BLS‐1 cells compared with the parent BLS‐1 cell line and an increase in heteroassociation FRET between HLA I and HLA II (compared with JY cells), suggesting a similar pattern of antigen presentation by the HLA‐DQ6 allele. Transmission electron microscopy (TEM) revealed that both HLA class I and class II molecules formed clusters at higher hierarchical levels on the tDQ6‐BLS‐1 cells, and the de novo synthesized HLA DQ molecules did not intersperse with HLA class I islands. These observations could be important in understanding the fine tuning of the immune response.},
	keywords = {FRET; TEM; MHC; immunogold labeling; BLS-1; HLA-DQ6},
	year = {2023},
	eissn = {1552-4930},
	pages = {978-991}
}

@article{MTMT:3352923,
	title = {Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin},
	url = {https://m2.mtmt.hu/api/publication/3352923},
	author = {Hrubi, Edit and Imre, László and Robaszkiewicz, A and Virág, László and Kerenyi, F and S. Nagy, Krisztina and Varga, Gábor and Jenei, Attila and Hegedűs, Csaba},
	doi = {10.1007/s13577-018-0202-5},
	journal-iso = {HUM CELL},
	journal = {HUMAN CELL: THE OFFICIAL JOURNAL OF THE JAPAN HUMAN CELL SOCIETY},
	volume = {31},
	unique-id = {3352923},
	issn = {0914-7470},
	abstract = {Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system to investigate the osteogenic effect of BMP-2 in vitro in a standardized manner still needs to be defined. The aim of this study was to examine the effect of BMP-2 on the cell proliferation and osteogenic differentiation of human osteogenic progenitors of various origins: dental pulp stem cells (DPSC), human osteosarcoma cell line (Saos-2) and human embryonic palatal mesenchymal cell line (HEPM). For induction of osteogenic differentiation, cell culture medium was supplemented with BMP-2 homodimer alone or in combination with conventionally used differentiation inducing agents. Differentiation was monitored for 6-18 days. To assess differentiation, proliferation rate, alkaline phosphatase activity, calcium deposition and the expression level of osteogenic differentiation marker genes (Runx2, BMP-2) were measured. BMP-2 inhibited cell proliferation in a concentration and time-dependent manner. In a concentration which caused maximal cell proliferation, BMP-2 did not induce osteogenic differentiation in any of the tested systems. However, it had a synergistic effect with the osteoinductive medium in both DPSC and Saos-2, but not in HEPM cells. We also found that the differentiation process was faster in Saos-2 than in DPSCs. Osteogenic differentiation could not be induced in the osteoblast progenitor HEPM cells. Our data suggest that in a concentration that inhibits proliferation the differentiation inducing effect of BMP-2 is evident only in the presence of permissive osteoinductive components. beta-glycerophosphate, was identified interacting with BMP-2 in a synergistic manner.},
	year = {2018},
	eissn = {1749-0774},
	pages = {139-148},
	orcid-numbers = {S. Nagy, Krisztina/0000-0002-4942-2947; Varga, Gábor/0000-0002-5506-8198}
}

@article{MTMT:3278641,
	title = {A fogászatban alkalmazható keresztkötött hialuronsav alapú hidrogélrendszerek szintézise és hatóanyag leadásának vizsgálata},
	url = {https://m2.mtmt.hu/api/publication/3278641},
	author = {Rente, Tünde and Bakó, József and Bágyi, Kinga, Ágnes and Jenei, Attila and Hegedűs, Csaba},
	doi = {10.33891/FSZ.110.3.82-87},
	journal-iso = {FOGORV SZLE},
	journal = {FOGORVOSI SZEMLE},
	volume = {110},
	unique-id = {3278641},
	issn = {0015-5314},
	year = {2017},
	eissn = {2498-8170},
	pages = {82-87}
}

@misc{MTMT:30326167,
	title = {Béta-trikálcium-foszfát (ß-TCP) funkcionális tulajdonságainak javítása hiarulonsav alapú hidrogél alkalmazásával},
	url = {https://m2.mtmt.hu/api/publication/30326167},
	author = {Rente, Tünde and Jenei, Attila and Hegedűs, Csaba},
	unique-id = {30326167},
	year = {2017},
	pages = {31}
}

@misc{MTMT:30330184,
	title = {A Kalcium szerepe a csontregeneráció szabályozásában},
	url = {https://m2.mtmt.hu/api/publication/30330184},
	author = {Rente, Tünde and Jenei, Attila and Hegedűs, Csaba},
	unique-id = {30330184},
	year = {2016},
	pages = {79}
}

@article{MTMT:2871115,
	title = {Understanding FRET as a research tool for cellular studies},
	url = {https://m2.mtmt.hu/api/publication/2871115},
	author = {Shrestha, Dilip and Jenei, Attila and Nagy, Péter and Vereb, György and Szöllősi, János},
	doi = {10.3390/ijms16046718},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {16},
	unique-id = {2871115},
	issn = {1661-6596},
	year = {2015},
	eissn = {1422-0067},
	pages = {6718-6756},
	orcid-numbers = {Nagy, Péter/0000-0002-7466-805X}
}

@article{MTMT:2495610,
	title = {CD1d favors MHC neighborhood, GM1 ganglioside proximity and low detergent sensitive membrane regions on the surface of B lymphocytes},
	url = {https://m2.mtmt.hu/api/publication/2495610},
	author = {Shrestha, D and Exley, MA and Vereb, György and Szöllősi, János and Jenei, Attila},
	doi = {10.1016/j.bbagen.2013.10.030},
	journal-iso = {BBA-GEN SUBJECTS},
	journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS},
	volume = {1840},
	unique-id = {2495610},
	issn = {0304-4165},
	abstract = {Background: Cluster of differentiation 1 (CD1) represents a family of proteins which is involved in lipid-based antigen presentation. Primarily, antigen presenting cells, like B cells, express CD1 proteins. Here, we examined the cell-surface distribution of CD1d, a subtype of CD1 receptors, on B lymphocytes. Methods: Fluorescence labeling methods, including fluorescence resonance energy transfer (FRET), were employed to investigate plasma membrane features of CD1d receptors. Results: High FRET efficiency was observed between CD1d and MHC I heavy chain (MHC I-HC), β2-microglobulin (β2m) and MHC II proteins in the plasma membrane. In addition, overexpression of CD1d reduced the expression of MHC II and increased the expression of MHC I-HC and β2m proteins on the cell-surface. Surprisingly, β2m dependent CD1d isoform constituted only ~ 15% of the total membrane CD1d proteins. Treatment of B cells with methyl-β-cyclodextrin (MβCD) / simvastatin caused protein rearrangement; however, FRET demonstrated only minimal effect of these chemicals on the association between CD1d and GM1 ganglioside on cell-surface. Likewise, a modest effect was only observed in a co-culture assay between MβCD/simvastatin treated C1R-CD1d cells and invariant natural killer T cells on measuring secreted cytokines (IFNγ and IL4). Furthermore, CD1d rich regions were highly sensitive to low concentration of Triton X-100. Physical proximity between CD1d, MHC and GM1 molecules was also detected in the plasma membrane. Conclusions: An intricate relationship between CD1d, MHC, and lipid species was found on the membrane of human B cells. General significance: Organization of CD1d on the plasma membrane might be critical for its biological functions. © 2013 Elsevier B.V. All rights reserved.},
	keywords = {ARTICLE; FRET; human; membrane protein; protein localization; priority journal; controlled study; cellular distribution; human cell; antigen expression; interleukin 4; gamma interferon; major histocompatibility complex; major histocompatibility antigen class 2; protein lipid interaction; b lymphocyte; physical chemistry; cell surface; ganglioside GM1; RAFTS; protein modification; SIMVASTATIN; cytokine release; killer cell; beta cyclodextrin; cell assay; MHC; major histocompatibility antigen class 1; isoprotein; Detergent; beta 2 microglobulin; coculture; membrane structure; triton x 100; CD1d antigen; Methyl-β-cyclodextrin; CD1d},
	year = {2014},
	eissn = {1872-8006},
	pages = {667-680}
}

@article{MTMT:2564008,
	title = {Preparation and application of highly porous aerogel-based bioactive materials in dentistry},
	url = {https://m2.mtmt.hu/api/publication/2564008},
	author = {Kuttor, A and Szalóki, Melinda and Rente, Tünde and Kerényi, Farkas and Bakó, József and Fábián, István and Lázár, István and Jenei, Attila and Hegedűs, Csaba},
	doi = {10.1007/s11706-014-0231-2},
	journal-iso = {FRONT MATER SCI},
	journal = {FRONTIERS OF MATERIALS SCIENCE},
	volume = {8},
	unique-id = {2564008},
	issn = {2095-025X},
	abstract = {In this study, the possibility of preparation and application of highly porous silica aerogel-based bioactive materials are presented. The aerogel was combined with hydroxyapatite and β-tricalcium phosphate as bioactive and osteoinductive agents. The porosity of aerogels was in the mesoporous region with a maximum pore diameter of 7.4 and 12.7 nm for the composite materials. The newly developed bioactive materials were characterized by scanning electron microscopy. The in vitro biological effect of these modified surfaces was also tested on SAOS-2 osteogenic sarcoma cells by confocal laser scanning microscopy. © 2014 Higher Education Press and Springer-Verlag Berlin Heidelberg.},
	keywords = {Aerogel; Sol-gel technique; SAOS-2 cell; bioactive material},
	year = {2014},
	eissn = {2095-0268},
	pages = {46-52},
	orcid-numbers = {Szalóki, Melinda/0000-0002-7052-0322; Lázár, István/0000-0001-6006-7782}
}

@misc{MTMT:30332573,
	title = {A kalcium- foszfát (CaP) szerepe a hatékony csontregenerációra},
	url = {https://m2.mtmt.hu/api/publication/30332573},
	author = {Rente, Tünde and Jenei, Attila and Hegedűs, Csaba},
	unique-id = {30332573},
	year = {2014},
	pages = {39}
}