TY - JOUR AU - Klátyik, Szandra AU - Takács, Eszter AU - Barócsi, Attila AU - Lenk, Sándor AU - Kocsányi, László AU - Darvas, Béla AU - Székács, András TI - Hormesis, the Individual and Combined Phytotoxicity of the Components of Glyphosate-Based Formulations on Algal Growth and Photosynthetic Activity JF - TOXICS J2 - TOXICS VL - 12 PY - 2024 IS - 4 SP - 257 SN - 2305-6304 DO - 10.3390/toxics12040257 UR - https://m2.mtmt.hu/api/publication/34764981 ID - 34764981 AB - The occurrence of the market-leading glyphosate active ingredient in surface waters is a globally observed phenomenon. Although co-formulants in pesticide formulations were considered inactive components from the aspects of the required main biological effect of the pesticide, several studies have proven the high individual toxicity of formulating agents, as well as the enhanced combined toxicity of the active ingredients and other components. Since the majority of active ingredients are present in the form of chemical mixtures in our environment, the possible combined toxicity between active ingredients and co-formulants is particularly important. To assess the individual and combined phytotoxicity of the components, glyphosate was tested in the form of pure active ingredient (glyphosate isopropylammonium salt) and herbicide formulations (Roundup Classic and Medallon Premium) formulated with a mixture of polyethoxylated tallow amines (POEA) or alkyl polyglucosides (APG), respectively. The order of acute toxicity was as follows for Roundup Classic: glyphosate < herbicide formulation < POEA. However, the following order was demonstrated for Medallon Premium: herbicide formulation < glyphosate < APG. Increased photosynthetic activity was detected after the exposure to the formulation (1.5–5.8 mg glyphosate/L and 0.5–2.2 mg POEA/L) and its components individually (glyphosate: 13–27.2 mg/L, POEA: 0.6–4.8 mg/L), which indicates hormetic effects. However, decreased photosynthetic activity was detected at higher concentrations of POEA (19.2 mg/L) and Roundup Classic (11.6–50.6 mg glyphosate/L). Differences were demonstrated in the sensitivity of the selected algae species and, in addition to the individual and combined toxicity of the components presented in the glyphosate-based herbicides. Both of the observed inhibitory and stimulating effects can adversely affect the aquatic ecosystems and water quality of surface waters. LA - English DB - MTMT ER - TY - JOUR AU - Takács, Eszter AU - Lázár, Diána AU - Siakwa, Augustine AU - Klátyik, Szandra AU - Mörtl, Mária AU - Kocsányi, László AU - Barócsi, Attila AU - Lenk, Sándor AU - Lengyel, Edina AU - Székács, András TI - Ecotoxicological Evaluation of Safener and Antimicrobial Additives in Isoxaflutole-Based Herbicide Formulations JF - TOXICS J2 - TOXICS VL - 12 PY - 2024 IS - 4 SP - 238 SN - 2305-6304 DO - 10.3390/toxics12040238 UR - https://m2.mtmt.hu/api/publication/34756893 ID - 34756893 AB - The environmental load by isoxaflutole and its formulated herbicide products has increasingly become apparent because, after the ban of atrazine, isoxaflutole has become its replacement active ingredient (a.i.). Obtaining information regarding the fate of this a.i. in environmental matrices and its ecotoxicological effects on aquatic organisms is essential for the risk assessment of the herbicide. In this study, the effects of Merlin Flexx- and Merlin WG75 formulated isoxaflutole-based herbicide products and two selected additives (cyprosulfamide safener and 1,2-benzisothiazol-3(2H)-one antimicrobial agent) were investigated on Raphidocelis subcapitata in growth inhibition assays. In ecotoxicological tests, two conventional (optical density and chlorophyll-a content) and two induced fluorescence-based (Fv*/Fp: efficiency of the photosystem PSII and Rfd* changes in the observed ratio of fluorescence decrease) endpoints were determined by UV-spectrophotometer and by our FluoroMeter Module, respectively. Furthermore, dissipation of isoxaflutole alone and in its formulated products was examined by an HPLC-UV method. In ecotoxicological assays, the fluorescence-based Rfd* was observed as the most sensitive endpoint. In this study, the effects of the safener cyprosulfamide and the antimicrobial agent 1,2-benzisothiazol-3(2H)-one on R. subcapitata is firstly reported. The results indicated that the isoxaflutole-equivalent toxicity of the mixture of the isoxaflutole–safener–antimicrobial agent triggered lower toxicity (EC50 = 2.81 ± 0.22 mg/L) compared to the individual effect of the a.i. (EC50 = 0.02 ± 0.00 mg/L). The Merlin Flexx formulation (EC50 = 27.04 ± 1.41 mg/L) was found to be approximately 50-fold less toxic than Merlin WG75, which can be explained by the different chemical characteristics and quantity of additives in them. The additives influenced the dissipation of the a.i. in Z8 medium, as the DT50 value decreased by approximately 1.2- and 3.5-fold under light and dark conditions, respectively. LA - English DB - MTMT ER - TY - JOUR AU - Czene, Szabolcs AU - Jegenyés, Nikoletta AU - Homokiné Krafcsik, Olga AU - Lenk, Sándor AU - Czigány, Zsolt AU - Bortel, Gábor AU - Kamarás, Katalin AU - Rohonczy, János AU - Beke, Dávid AU - Gali, Ádám TI - Amino-Termination of Silicon Carbide Nanoparticles JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 13 PG - 17 SN - 2079-4991 DO - 10.3390/nano13131953 UR - https://m2.mtmt.hu/api/publication/34040657 ID - 34040657 AB - Silicon carbide nanoparticles (SiC NPs) are promising inorganic molecular-sized fluorescent biomarkers. It is imperative to develop methods to functionalize SiC NPs for certain biological applications. One possible route is to form amino groups on the surface, which can be readily used to attach target biomolecules. Here, we report direct amino-termination of aqueous SiC NPs. We demonstrate the applicability of the amino-terminated SiC NPs by attaching bovine serum albumin as a model for functionalization. We monitor the optical properties of the SiC NPs in this process and find that the fluorescence intensity is very sensitive to surface termination. Our finding may have implications for a few nanometers sized SiC NPs containing paramagnetic color centers with optically read electron spins. LA - English DB - MTMT ER - TY - JOUR AU - Koppány, Ferenc AU - Csomó, Krisztián Benedek AU - Varmuzsa, Edvárd Márton AU - Bognár, Eszter AU - Pelyhe, Liza AU - Nagy, Péter AU - Kientzl, Imre AU - Szabó, Dániel AU - Weszl, Miklós AU - Dobos, Gábor AU - Lenk, Sándor AU - Erdei, Gábor AU - Kiss, Gábor AU - Nagy, Lilien AU - Sréter, Attila AU - Belik, Andrea AU - Tóth, Zsuzsanna AU - Vág, János AU - Joób-Fancsaly, Árpád AU - Németh, Zsolt TI - Enhancement of Hydrophilicity of Nano-Pitted TiO2 Surface Using Phosphoric Acid Etching JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 3 PG - 9 SN - 2079-4991 DO - 10.3390/nano13030511 UR - https://m2.mtmt.hu/api/publication/33607227 ID - 33607227 N1 - Funding Agency and Grant Number: REA [FP7-SME-2013-606624] Funding text: This research was funded by REA in the 7th Framework Program within the funding scheme of SME Instrument under the scope of the NANOTI Project (FP7-SME-2013-606624). AB - Our research group developed a novel nano-pitted (NP) TiO2 surface on grade 2 titanium that showed good mechanical, osteogenic, and antibacterial properties; however, it showed weak hydrophilicity. Our objective was to develop a surface treatment method to enhance the hydrophilicity of the NP TiO2 surface without the destruction of the nano-topography. The effects of dilute and concentrated orthophosphoric (H3PO4) and nitric acids were investigated on wettability using contact angle measurement. Optical profilometry and atomic force microscopy were used for surface roughness measurement. The chemical composition of the TiO2 surface and the oxidation state of Ti was investigated using X-ray photoelectron spectroscopy. The ccH3PO4 treatment significantly increased the wettability of the NP TiO2 surfaces (30°) compared to the untreated control (88°). The quantity of the absorbed phosphorus significantly increased following ccH3PO4 treatment compared to the control and caused the oxidation state of titanium to decrease (Ti4+ → Ti3+). Owing to its simplicity and robustness the presented surface treatment method may be utilized in the industrial-scale manufacturing of titanium implants. LA - English DB - MTMT ER - TY - JOUR AU - Lázár, Diána AU - Takács, Eszter AU - Mörtl, Mária AU - Klátyik, Szandra AU - Barócsi, Attila AU - Kocsányi, László AU - Lenk, Sándor AU - Domjan, László AU - Szarvas , Gábor AU - Lengyel, Edina AU - Székács, András TI - Application of a Fluorescence-Based Instrument Prototype for Chlorophyll Measurements and Its Utility in an Herbicide Algal Ecotoxicity Assay JF - WATER J2 - WATER-SUI VL - 15 PY - 2023 IS - 10 PG - 21 SN - 2073-4441 DO - 10.3390/w15101866 UR - https://m2.mtmt.hu/api/publication/33832426 ID - 33832426 N1 - Agro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, Budapest, H-1022, Hungary Limnology Research Group, Center for Natural Science, University of Pannonia, Egyetem u. 10, Veszprém, H-8200, Hungary Department of Atomic Physics, Institute of Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Optimal Optik Ltd, Dayka Gábor u. 6/B, Budapest, H-1118, Hungary Limnoecology Research Group, ELKH-PE, Egyetem u. 10, Veszprém, H-8200, Hungary Agrotechnology National Laboratory, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Páter K. u. 1., Gödöllő, H-2100, Hungary Export Date: 15 June 2023 Correspondence Address: Székács, A.; Agro-Environmental Research Centre, Herman O. út 15, Hungary; email: szekacs.andras@uni-mate.hu AB - Project Aquafluosense was designed to develop prototypes for a modular fluorescence-based instrumental setup for in situ measurement of major water quality parameters. A fluorometer was developed for algal density estimation based on the fluorescent excitation of chlorophyll. The appropriate type of sample holder microplate was determined, along with the need for dark acclimation, prior to the measurements during the instrument’s development. Model species of green (Raphidocelis subcapitata) and blue-green alga (Microcystis aeruginosa) were applied in forms of pure monocultures and their mixtures, and improved analytical limits of detection were achieved (3.70 × 103 cell/mL and 1.13 × 105 for R. subcapitata and M. aeruginosa, respectively). The fluorescence-based determination of algal density was validated by conventional methods, such as cell counting in a Bürker chamber, optical density measurement, and chlorophyll extraction with ethanol. The signals obtained by the fluorometer correlated well with the conventional methods. Pearson r coefficients (applied where the correlation was linear) were ≥0.988 and Spearman ρ coefficients (applied where the correlation was not linear) were >0.976, indicating a strong and positive correlation. The applicability of the developed fluorometer was demonstrated in a growth inhibition ecotoxicity assay on R. subcapitata using the herbicide active ingredient isoxaflutole. During the assay, light intensity (continuous, 104.9 ± 14.9 µE/m2/s), temperature (22 ± 2 °C), pH of algal media (pH = 6–7 for Zehnder and Allen media, as well), and intensity of stirring (continuous, 100 rpm) were controlled. The results indicated that the FluoroMeter Module is applicable for screening algal toxicity: the observed ratio of fluorescence decrease determined by fluorescence induction provided significantly lower toxicity values (EC50: 0.015 ± 0.001 µg/mL) compared to values determined by the optical density (EC50: 0.034 ± 0.004 µg/mL) and chlorophyll a content (EC50: 0.033 ± 0.000 µg/mL). LA - English DB - MTMT ER - TY - JOUR AU - Merkel, Dániel AU - Sájerman, K. AU - Váczi, Tamás AU - Lenk, Sándor AU - Hegedűs, Gergő AU - Sajti, Szilárd Mihály AU - Németh, Attila AU - Gracheva, Maria AU - Petrik, Péter AU - Mukherjee, Deshabrato AU - Horváth, Zsolt Endre AU - Nagy, Dénes Lajos AU - Lengyel, Attila TI - Laser irradiation effects in FeRh thin film JF - MATERIALS RESEARCH EXPRESS J2 - MATER RES EXPRESS VL - 10 PY - 2023 IS - 7 PG - 7 SN - 2053-1591 DO - 10.1088/2053-1591/ace4a3 UR - https://m2.mtmt.hu/api/publication/34536298 ID - 34536298 LA - English DB - MTMT ER - TY - JOUR AU - Sági-Kazár, Máté AU - Sárvári, Éva AU - Cseh, Barnabás AU - Illés, Levente AU - May, Zoltán AU - Hegedűs, Csaba AU - Barócsi, Attila AU - Lenk, Sándor AU - Solymosi, Katalin AU - Solti, Ádám TI - Iron uptake of etioplasts is independent from photosynthesis but applies the reduction based strategy JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 14 PY - 2023 PG - 17 SN - 1664-462X DO - 10.3389/fpls.2023.1227811 UR - https://m2.mtmt.hu/api/publication/34077350 ID - 34077350 N1 - Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Doctoral School of Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Umeå, Sweden Department of Atomic Physics, Budapest University of Technology and Economics, Budapest, Hungary Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, Hungary Department of Plant Anatomy, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Export Date: 4 September 2023 Correspondence Address: Solti, Á.; Department of Plant Physiology and Molecular Plant Biology, Hungary; email: adam.solti@ttk.elte.hu AB - Iron (Fe) is one of the most important cofactors in the photosynthetic apparatus, and its uptake by chloroplasts have been also associated with the operation of the photosynthetic electron transport chain during reduction-based plastidial Fe uptake. Therefore, plastidial Fe uptake was considered not to be operational in the absence of the photosynthetic activity. Nevertheless, Fe is also required for enzymatic functions unrelated to photosynthesis, highlighting the importance of Fe acquisition by nonphotosynthetic plastids. Yet, it remains unclear how these plastids acquire Fe in the absence of photosynthetic function. Furthermore, plastids of etiolated tissues should already possess the ability to acquire Fe, since the biosynthesis of thylakoid membrane complexes requires a massive amount of readily available Fe. Thus, we aimed to investigate whether the reduction based plastidial Fe uptake solely relies on the functioning photosynthetic apparatus. We used Savoy cabbage plant as a model, which develops natural etiolation in the inner leaves of the heads due to the shading of the outer leaf layers. Foliar and plastidial Fe content of Savoy cabbage leaves decreased towards the inner leaf layers. The leaves of the innermost leaf layers proved to be etiolated, containing etioplasts which lacked the photosynthetic machinery and thus were photosynthetically inactive. However, we discovered that these etioplasts contained, and were able to take up Fe. Although, the relative transcript abundance of genes associated with plastidial Fe uptake and homeostasis decreased towards the inner leaf layers, both ferric chelate reductase FRO7 transcripts and activity was detected in the innermost leaf layer. Additionally, a significant NADP(H) pool and NAD(P)H dehydrogenase activity was detected in the etioplasts of the innermost leaf layer, indicating the presence of the reducing capacity that likely supports the reduction based Fe uptake of etioplasts. Based on these findings, the reduction based plastidial Fe acquisition should not be considered exclusively dependent on the photosynthetic functions. LA - English DB - MTMT ER - TY - JOUR AU - Saláta, József AU - Fehér, Dóra AU - Lenk, Sándor AU - Ujhelyi, Ferenc AU - Borbély, Judit AU - Hermann, Péter AU - Ábrám, Emese TI - Monolitikus cirkónium-dioxid fogpótlások anyagának spektrofotometriai vizsgálata JF - FOGORVOSI SZEMLE J2 - FOGORV SZLE VL - 116 PY - 2023 IS - 1 SP - 2 EP - 8 PG - 7 SN - 0015-5314 DO - 10.33891/FSZ.116.1.2-8 UR - https://m2.mtmt.hu/api/publication/33707930 ID - 33707930 AB - Bevezetés: Világszerte egyre szélesebb körben alkalmazzák a cirkónium-dioxid kerámiákat monolitikus fogpótlások anyagaként. A teljes kerámiarendszerek esetén a végleges restaurátum esztétikáját a kerámiaanyag színén túl olyan további tényezők is befolyásolják, mint a csonkszín, a kerámia vastagsága és transzlucenciája, illetve a cement színe és rétegvastagsága. Célkitűzés: A Semmelweis Egyetem Fogpótlástani Klinikájának és a Budapesti Műszaki és Gazdaságtudományi Egyetem Atomfizika Tanszékének közös in vitro kísérletének célja tanulmányozni, hogy a különböző árnyalatú és vastagságú monolitikus cirkónium-dioxid kerámiák optikai tulajdonságait milyen módon befolyásolja a csonkszín és a cementszín. Anyag és módszer: Vizsgálatunkhoz kétféle árnyalatú (A2P1, WHITE), ötféle rétegvastagságú cirkónium-dioxid mintát (Erran Tech), háromféle próbacementet (Variolink Esthetic Try-In Paste, Ivoclar Vivadent), kilencféle csonkanyagot (hat VITA Simulate, három fémtartalmú csonkanyag) használtunk fel. Méréseinket a Budapesti Műszaki és Gazdaságtudományi Egyetem Atomfizika Tanszékén lévő PerkinElmer LAMBDA 1050 UV/Vis/NIR spektrofotométerrel végeztük el. A színkülönbség (ΔE) számításához a CIEDE2000 képletet alkalmaztuk. Eredmények: 0,5 mm vastagságú A2P1-minták esetén az átlagos ΔE xΔE = 4,10 (σΔE = 2,91); 2,5 mm vastagság mellett xΔE = 1,88 (σΔE = 0,67). 0,5 mm vastagságú WHITE-minták esetén az átlagos ΔE xΔE = 6,40 (σΔE = 2,75); 2,5 mm vastagság mellett xΔE = 5,46 (σΔE = 0,79). Megbeszélés: A kutatás rámutatott arra, hogy a cirkónium-dioxid minták színezettsége és rétegvastagsága nagyban befolyásolja a fedőképességet, illetve 1,5 mm kerámiavastagság mellett a próbacementek szignifikáns színeltérést okoznak. A színezetlen cirkónium-dioxidok színét kevésbé befolyásolja a csonkszín, különösen 1,5 mm rétegvastagság felett. Következtetés: Elszíneződött csonkok monolitikus cirkónium-dioxid fogpótlással való fedése esetén a rétegvastagságnövelésével esztétikusabb végeredményt tudunk elérni a vizsgált színezett cirkónium-dioxid alkalmazása mellett. LA - Hungarian DB - MTMT ER - TY - CHAP AU - Lázár, Diána AU - Klátyik, Szandra AU - Takács, Eszter AU - Barócsi, Attila AU - László, Kocsányi AU - Lenk, Sándor AU - László, Domján AU - Gábor, Szarvas AU - Edina, Lengyel AU - Székács, András ED - Alapi, Tünde ED - Berkecz, Róbert ED - Ilisz, István TI - APPLICATION OF AN INDUCED FLUOROMETRY-BASED METHOD IN ALGAL GROWTH INHIBITION TESTS T2 - Proceedings of the 28th International Symposium on Analytical and Environmental Problems PB - University of Szeged CY - Szeged SN - 9789633069042 PY - 2022 SP - 211 EP - 215 PG - 5 UR - https://m2.mtmt.hu/api/publication/33291485 ID - 33291485 LA - English DB - MTMT ER - TY - CHAP AU - Takács, Eszter AU - Gémes, Borbála Letícia AU - Fanni, Szendrei AU - Csaba, Keszei AU - Barócsi, Attila AU - Lenk, Sándor AU - László, Domján AU - Mörtl, Mária AU - Klátyik, Szandra AU - Székács, András ED - Alapi, Tünde ED - Berkecz, Róbert ED - Ilisz, István TI - ENZYME-LINKED FLUORESCENT IMMUNOASSAY FOR MONITORING THE HERBICIDE ACTIVE INGREDIENT GLYPHOSATE IN WATER T2 - Proceedings of the 28th International Symposium on Analytical and Environmental Problems PB - University of Szeged CY - Szeged SN - 9789633069042 PY - 2022 SP - 350 EP - 354 PG - 5 UR - https://m2.mtmt.hu/api/publication/33291661 ID - 33291661 LA - English DB - MTMT ER - TY - JOUR AU - Lázár, Diána AU - Klátyik, Szandra AU - Takács, Eszter AU - Kocsányi, László AU - Barócsi, Attila AU - Lenk, Sándor AU - Domján, László AU - Szarvas, Gábor AU - Lengyel, Edina AU - Székács, András TI - Az isoxaflutole gyomirtószer-hatóanyag és formázott készítményeinek ökotoxikológiai vizsgálata Raphidochelis subcapitata algafajon JF - ÖKOTOXIKOLÓGIA J2 - ÖKOTOXIKOLÓGIA VL - 4 PY - 2022 IS - 3-4 SP - 39 EP - 47 PG - 9 SN - 2732-2556 UR - https://m2.mtmt.hu/api/publication/33290350 ID - 33290350 LA - Hungarian DB - MTMT ER - TY - BOOK AU - Lenk, Sándor AU - Illés, Levente AU - Steinbach, Ferenc AU - Mayer, Péter AU - Barócsi, Attila AU - Sági-Kazár, Máté AU - Solymosi, Katalin AU - Solti, Ádám TI - Növénybiológiai minták fluoreszcencia lecsengési idejének vizsgálata PY - 2022 UR - https://m2.mtmt.hu/api/publication/33874519 ID - 33874519 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Takács, Eszter AU - Gémes, Borbála Letícia AU - Szendrei, F. AU - Keszei, C. AU - Barócsi, Attila AU - Lenk, Sándor AU - Domján, L. AU - Mörtl, Mária AU - Székács, András TI - Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 19 PG - 12 SN - 1420-3049 DO - 10.3390/molecules27196514 UR - https://m2.mtmt.hu/api/publication/33189815 ID - 33189815 N1 - Agro-Environmental Research Centre, Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Herman O. út 15, Budapest, H-1022, Hungary Institute of Isotopes Co. Ltd, Konkoly-Thege Miklós út 29-33, Budapest, H-1121, Hungary Department of Atomic Physics, Institute of Physics, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Optimal Optik Ltd, Dayka Gábor u. 6/B, Budapest, H-1118, Hungary Export Date: 25 October 2022 CODEN: MOLEF Correspondence Address: Takács, E.; Agro-Environmental Research Centre, Herman O. út 15, Hungary; email: takacs.eszter84@uni-mate.hu AB - An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds. LA - English DB - MTMT ER - TY - JOUR AU - Takács, Eszter AU - Gémes, Borbála Letícia AU - Szendrei, Fanni AU - Keszei, Csaba AU - Barócsi, Attila AU - Lenk, Sándor AU - Domján, László AU - Mörtl, Mária AU - Klátyik, Szandra AU - Székács, András TI - Enzimjelzéses fluoreszcens immunanalitikai módszer a glyphosate gyomirtószer-hatóanyag kimutatására felszíni vízben JF - ÖKOTOXIKOLÓGIA J2 - ÖKOTOXIKOLÓGIA VL - 4 PY - 2022 IS - 3-4 SP - 48 EP - 53 PG - 6 SN - 2732-2556 UR - https://m2.mtmt.hu/api/publication/33290406 ID - 33290406 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Gémes, Borbála Letícia AU - Takács, Eszter AU - Gádoros, Patrik AU - Barócsi, Attila AU - Kocsányi, László AU - Lenk, Sándor AU - Csákányi, Attila AU - Kautny, Szabolcs AU - Domjan, László AU - Szarvas , Gábor AU - Adányiné Kisbocskói, Nóra AU - Nabok, Alexei AU - Mörtl, Mária AU - Székács, András TI - Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water JF - TOXINS J2 - TOXINS VL - 13 PY - 2021 IS - 3 PG - 17 SN - 2072-6651 DO - 10.3390/toxins13030182 UR - https://m2.mtmt.hu/api/publication/31931250 ID - 31931250 AB - Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detection of several environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by several plant pathogenic Fusarium species, has recently been identified as an emerging pollutant in surface water, presenting a hazard to aquatic ecosystems. Due to its physico-chemical properties, detection of ZON at low concentrations in surface water is a challenging task. The 96-well microplate-based fluorescence instrument is capable of detecting ZON in the concentration range of 0.09–400 ng/mL. The sensitivity and accuracy of the analytical method has been demonstrated by a comparative assessment with detection by high-performance liquid chromatography and by total internal reflection ellipsometry. The limit of detection of the method, 0.09 ng/mL, falls in the low range compared to the other reported immunoassays, but the main advantage of this ELFIA method is its efficacy in combined in situ applications for determination of various important water quality parameters detectable by induced fluorimerty—e.g., total organic carbon content, algal density or the level of other organic micropollutants detectable by immunofluorimetry. In addition, the immunofluorescence module can readily be expanded to other target analytes if proper antibodies are available for detection. LA - English DB - MTMT ER - TY - CHAP AU - Lázár, Diána AU - Takács, Eszter AU - Klátyik, Szandra AU - Lenk, Sándor AU - Barócsi, Attila AU - Kocsányi, László AU - Adányiné Kisbocskói, Nóra AU - Székács, András ED - Jakab, Gusztáv ED - Csengeri, Erzsébet TI - Fluoriméter fejlesztése klorofill in situ mérésére T2 - MINDEN CSEPP SZÁMÍT: IV. VÍZTUDOMÁNYI NEMZETKÖZI KONFERENCIA = EVERY DROP COUNTS: IVth INTERNATIONAL CONFERENCE ON WATER SCIENCES PB - Hungarian University of Agriculture and Life Sciences, Institute of Environmental Sciences Department of Irrigation and Land Improvement CY - Szarvas SN - 9789632699523 PY - 2021 SP - 10 EP - 17 PG - 8 UR - https://m2.mtmt.hu/api/publication/32708152 ID - 32708152 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Lenk, Sándor AU - Sági-Kazár, Máté AU - Illés, Levente AU - Solymosi, Katalin AU - Solti, Ádám AU - Barócsi, Attila ED - Földi, Péter ED - Magashegyi, István TI - Növényi minták fluoreszcencia lecsengési idejének vizsgálata T2 - Kvantumelektronika 2021 PB - Szegedi Tudományegyetem TTIK Fizikai Intézet CY - Szeged SN - 9789633067758 PY - 2021 SP - 123 EP - 127 PG - 5 DO - 10.14232/kvantumelektronika.9.24 UR - https://m2.mtmt.hu/api/publication/31852843 ID - 31852843 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Molnár, János AU - Zuba, Z. AU - Sepsi, Örs AU - Ujhelyi, Ferenc AU - Erdei, Gábor AU - Lenk, Sándor AU - Kállay-Menyhárd, Alfréd TI - Structural investigation of semicrystalline polymers JF - POLYMER TESTING J2 - POLYM TEST VL - 95 PY - 2021 SN - 0142-9418 DO - 10.1016/j.polymertesting.2021.107098 UR - https://m2.mtmt.hu/api/publication/31875450 ID - 31875450 N1 - Department of Physical Chemistry and Materials Science, Laboratory of Plastics and Rubber Technology, Budapest University of Technology and Economics, Rkp. 3. H. ép. I, Budapest, Műegyetem H-1111, Hungary Department of Atomic Physics, Budapest University of Technology and Economics, Budafoki 8, Budapest, H-1111, Hungary Export Date: 19 May 2021 CODEN: POTED Correspondence Address: Molnár, J.; Department of Physical Chemistry and Materials Science, Rkp. 3. H. ép. I, Hungary; email: molnar.janos@mail.bme.hu AB - This work demonstrates the most widely used characterization methods and techniques of the supermolecular and lamellar structure of semicrystalline polymers. Polarized optical microscopy (POM) equipped with a hot-stage (thermo-optical microscopy, TOM), brightfield microscopy (BF), darkfield microscopy (DF), digital image processing techniques, optical profilometry (OP), scanning electron microscopy (SEM), and atomic force microscopy (AFM) were used as investigation techniques. The same iPP grade was used with different sample preparation techniques to compare these methods. The advantages and drawbacks of the sample preparation and investigation methods were discussed. The results show how the introduced techniques could reveal different kinds of information, and it is also shown how the experimental techniques should be matched to the goals of a structural study. LA - English DB - MTMT ER - TY - CHAP AU - Sági-Kazár, Máté AU - Lenk, Sándor AU - Cseh, Barnabás AU - Illés, Levente AU - Barócsi, Attila AU - Sárvári, Éva AU - Solymosi, Katalin AU - Solti, Ádám ED - Györgyey, János TI - A redukción alapuló plasztidiális vasfelvétel mintázata és szabályozása T2 - XIII. Magyar Növénybiológiai Kongresszus: Összefoglaló kötet PB - Szegedi Biológiai Kutatóközpont CY - Szeged SN - 9786150123509 PY - 2021 UR - https://m2.mtmt.hu/api/publication/32182293 ID - 32182293 LA - Hungarian DB - MTMT ER - TY - CONF AU - Sági-Kazár, Máté AU - Lenk, Sándor AU - Sárvári, Éva AU - Hegedűs, Csaba AU - Barócsi, Attila AU - Solymosi, Katalin AU - Solti, Ádám TI - Molecular background of iron uptake mechanisms of non-photosynthetic plastids T2 - 19th Congress of the European Society for Photobiology - Book of Abstracts PY - 2021 SP - SP-8.4.6 UR - https://m2.mtmt.hu/api/publication/32182182 ID - 32182182 LA - English DB - MTMT ER -