TY - JOUR AU - Héja, László AU - Simon, Ágnes AU - Kardos, Julianna TI - Simulation of gap junction formation reveals critical role of Cys disulfide redox state in connexin hemichannel docking JF - CELL COMMUNICATION AND SIGNALING J2 - CELL COMM SIGN VL - 22 PY - 2024 IS - 1 SN - 1478-811X DO - 10.1186/s12964-023-01439-z UR - https://m2.mtmt.hu/api/publication/34768350 ID - 34768350 LA - English DB - MTMT ER - TY - JOUR AU - Héja, László AU - Kardos, Julianna TI - GABA fluctuations driven by astrocytic Glu-GABA exchange explain synaptic acuity JF - Cell Signaling J2 - Cell Signal VL - 1 PY - 2023 IS - 1 SP - 76 EP - 80 PG - 5 SN - 2837-8253 DO - 10.46439/signaling.1.016 UR - https://m2.mtmt.hu/api/publication/34718118 ID - 34718118 LA - English DB - MTMT ER - TY - JOUR AU - Héja, László AU - Simon, Ágnes AU - Szabó, Zsolt AU - Kardos, Julianna TI - Connexons coupling to gap junction channel: Potential role for extracellular protein stabilization centers JF - BIOMOLECULES J2 - BIOMOLECULES VL - 12 PY - 2022 IS - 1 SN - 2218-273X DO - 10.3390/biom12010049 UR - https://m2.mtmt.hu/api/publication/32612048 ID - 32612048 N1 - Export Date: 24 January 2022 Correspondence Address: Héja, L.; Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, Hungary; email: heja.laszlo@ttk.hu Chemicals/CAS: cystine, 24645-67-8, 56-89-3, 6020-39-9 Funding details: National Research, Development and Innovation Office, OTKA K124558 Funding text 1: Funding: This work was supported by National Research, Development and Innovation Office grant OTKA K124558. AB - Connexin (Cx) proteins establish intercellular gap junction channels (Cx GJCs) through coupling of two apposed hexameric Cx hemichannels (Cx HCs, connexons). Pre-and post-GJ interfaces consist of extracellular EL1 and EL2 loops, each with three conserved cysteines. Previously, we reported that known peptide inhibitors, mimicking a variety of Cx43 sequences, appear non-selective when binding to homomeric Cx43 vs. Cx36 GJC homology model subtypes. In pursuit of finding potentially Cx subtype-specific inhibitors of connexon-connexon coupling, we aimed at to understand better how the GJ interface is formed. Here we report on the discovery of Cx GJC subtype-specific protein stabilization centers (SCs) featuring GJ interface architecture. First, the Cx43 GJC homology model, embedded in two opposed membrane bilayers, has been devised. Next, we endorsed the fluctuation dynamics of SCs of the interface domain of Cx43 GJC by applying standard molecular dynamics under open and closed cystine disulfide bond (CS-SC) preconditions. The simulations confirmed the major role of of the unique trans-GJ SC pattern comprising conserved (55N, 56T) and non-conserved (57Q) residues of the apposed EL1 loops in the stabilization of the GJC complex. Importantly, clusters of SC patterns residing close to the GJ interface domain appear to orient the interface formation via the numerous SCs between EL1 and EL2. These include central54CS-S198C or61CS-S192C contacts with residues 53R, 54C, 55N, 197D, 199F or 64V, 191P, respectively. In addition, we revealed that GJC interface formation is favoured when the psi dihedral angle of the nearby 193P residue is stable around 180◦ and the interface SCs disappear when this angle moves to the 0◦ to −45◦ range. The potential of the association of non-conserved residues with SC motifs in connexon-connexon coupling makes the development of Cx subtype-specific inhibitors viable. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Skatchkov, Serguei N. AU - Szabó, Zsolt AU - Qahtan, Saif AU - Méndez-González, Miguel P. AU - Malpica-Nieves, Christian J. AU - Eaton, Misty J. AU - Kardos, Julianna AU - Héja, László TI - Putrescine Intensifies Glu/GABA Exchange Mechanism and Promotes Early Termination of Seizures JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 15 SN - 1661-6596 DO - 10.3390/ijms23158191 UR - https://m2.mtmt.hu/api/publication/33031938 ID - 33031938 N1 - Department of Biology, Savaria University Centre, ELTE Eötvös Loránd University, Károlyi Gáspár tér 4, Szombathely, 9700, Hungary Department of Physiology, Universidad Central del Caribe, Bayamon, 00960, Puerto Rico Department of Biochemistry, Universidad Central del Caribe, Bayamon, 00960, Puerto Rico Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, Budapest, 1117, Hungary School of Chemistry, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary College of Science, University of Al-Qadisiyah, Al-Diwaniyah, 58001, Iraq Natural Sciences Department, University of Puerto Rico in Aguadilla, Aguadilla, 00604, Puerto Rico Department of Science and Technology, Antilles Adventist University, Mayagüez, 00681, Puerto Rico Export Date: 25 October 2022 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Magyar Tudósok Körútja 2, Hungary; email: heja.laszlo@ttk.hu LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Zsolt AU - Skatchkov, Serguei N. AU - Veh, Rüdiger W. AU - Szabó, Zsolt AU - Németh, Krisztina AU - Szabó, Pál Tamás AU - Kardos, Julianna AU - Héja, László TI - Critical Role of Astrocytic Polyamine and GABA Metabolism in Epileptogenesis JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 15 PY - 2022 PG - 15 SN - 1662-5102 DO - 10.3389/fncel.2021.787319 UR - https://m2.mtmt.hu/api/publication/32578354 ID - 32578354 N1 - Department of Biology, ELTE Eötvös Loránd University, Savaria University Centre, Szombathely, Hungary Department of Physiology, Universidad Central Del Caribe, Bayamon, PR, United States Department of Biochemistry, Universidad Central Del Caribe, Bayamon, PR, United States Institut für Zell- und Neurobiologie, Centrum 2, Charité - Universitätsmedizin Berlin, Berlin, Germany Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, Hungary MS Metabolomics Research Group, Centre for Structural Study, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, Hungary Export Date: 28 February 2022 Correspondence Address: Skatchkov, S.N.; Department of Physiology, United States; email: serguei.skatchkov@uccaribe.edu Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.hu Chemicals/CAS: 4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid, 7365-45-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; acetonitrile, 75-05-8; alcohol, 64-17-5; alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid, 77521-29-0; ammonium sulfate, 7783-20-2; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; bicarbonate, 144-55-8, 71-52-3; calcium chloride, 10043-52-4; carbon dioxide, 124-38-9, 58561-67-4; diaminobenzidine, 7411-49-6, 91-95-2; dimethyl sulfoxide, 67-68-5; eflornithine, 67037-37-0, 70052-12-9; ethanolamine, 141-43-5; formic acid, 64-18-6, 71-47-6; gluconic acid, 133-42-6, 526-95-4, 66664-08-2; glucose, 50-99-7, 84778-64-3; glutaraldehyde, 111-30-8, 37245-61-7; histamine, 51-45-6, 56-92-8, 93443-21-1; hydrogen peroxide, 7722-84-1; imidazole, 1467-16-9, 288-32-4; immunoglobulin G, 97794-27-9; isoflurane, 26675-46-7; levetiracetam, 102767-28-2; lidocaine, 137-58-6, 24847-67-4, 56934-02-2, 73-78-9; lysine, 56-87-1, 6899-06-5, 70-54-2; magnesium sulfate, 7487-88-9; methyl green, 54327-10-5; n methyl dextro aspartic acid, 6384-92-5; nitrogen, 7727-37-9; ornithine, 70-26-8, 7006-33-9; ornithine decarboxylase, 9024-60-6; paraformaldehyde, 30525-89-4; peroxidase, 9003-99-0; picric acid, 14798-26-6, 88-89-1; potassium chloride, 7447-40-7; putrescine, 110-60-1, 333-93-7; sodium borohydride, 16940-66-2; sodium chloride, 7647-14-5, 23724-87-0, 49658-21-1; sodium dihydrogen phosphate, 7558-80-7, 7632-05-5; spermidine, 124-20-9, 334-50-9; sucrose, 122880-25-5, 57-50-1; water, 7732-18-5 Tradenames: 1100, Agilent, United States; Bioamp4; BX51WI, Olympus, Japan; DP30BW, Olympus, Japan; MX7500; QTRAP 6500, Sciex, United States; VT1000S, Leica, Germany Manufacturers: Vector, Germany; Tocris, United Kingdom; Egis, Hungary; TCI, Japan; Ivoclar, Liechtenstein; Sigma Aldrich, United StatesLeica, Germany; Olympus, Japan; Agilent, United States; Axon, United States; Sciex, United States; Sutter, United States; World Precision Instruments, United States Funding details: National Institutes of Health, NIH, NIMHD-PRCTRC-8U54MD007587-03 Funding details: National Institute of General Medical Sciences, NIGMS, G12MD007583 Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R15-NS-116478, RO1-NS-065201 Funding details: Magyar Tudományos Akadémia, MTA Funding details: National Research, Development and Innovation Office, OTKA K124558 Funding text 1: This work was supported by the National Research, Development and Innovation Office grant OTKA K124558 and by the National Institutes of Health grants: NIH NINDS RO1-NS-065201 (to SS), NIH NINDS R15-NS-116478 (to SS), and NIH NIMHD-PRCTRC-8U54MD007587-03 (to UCC and PMS consortium); NIH NIGMS G12MD007583 (UCC Core facilities). LH is a recipient of the János Bolyai Scholarship of the Hungarian Academy of Sciences. Funding text 2: This work was supported by the National Research, Development and Innovation Office grant OTKA K124558 and by the National Institutes of Health grants: NIH NINDS RO1-NS-065201 (to SS), NIH NINDS R15-NS-116478 (to SS), and NIH NIMHD-PRCTRC-8U54MD007587-03 (to UCC and PMS consortium); NIH NIGMS G12MD007583 (UCC Core facilities). LH is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. LA - English DB - MTMT ER - TY - JOUR AU - Héja, László AU - Szabó, Zsolt AU - Péter, Márton AU - Kardos, Julianna TI - Spontaneous Ca2+ Fluctuations Arise in Thin Astrocytic Processes With Real 3D Geometry JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 15 PY - 2021 SN - 1662-5102 DO - 10.3389/fncel.2021.617989 UR - https://m2.mtmt.hu/api/publication/31925857 ID - 31925857 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office [OTKA K124558]; Hungarian Academy of SciencesHungarian Academy of Sciences Funding text: This work was supported by National Research, Development, and Innovation Office grant OTKA K124558. LH is a recipient of the Janos Bolyai Scholarship of the Hungarian Academy of Sciences. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences (MTA), Budapest, Hungary Hevesy György PhD School of Chemistry, ELTE Eötvös Loránd University, Budapest, Hungary Cited By :1 Export Date: 29 July 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Funding details: Magyar Tudományos Akadémia, MTA Funding details: National Research, Development and Innovation Office, OTKA K124558 Funding text 1: We thank P?ter Somogyi FRS, FMedSci, Professor of Neurobiology, Department of Pharmacology, University of Oxford for valuable discussions. Funding. This work was supported by National Research, Development, and Innovation Office grant OTKA K124558. LH is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences (MTA), Budapest, Hungary Hevesy György PhD School of Chemistry, ELTE Eötvös Loránd University, Budapest, Hungary Cited By :2 Export Date: 23 September 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; calcium ion, 14127-61-8; sodium ion, 17341-25-2 Funding details: Magyar Tudományos Akadémia, MTA Funding details: National Research, Development and Innovation Office, OTKA K124558 Funding text 1: We thank P?ter Somogyi FRS, FMedSci, Professor of Neurobiology, Department of Pharmacology, University of Oxford for valuable discussions. Funding. This work was supported by National Research, Development, and Innovation Office grant OTKA K124558. LH is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Zsolt AU - Péter, Márton AU - Héja, László AU - Kardos, Julianna TI - Dual Role for Astroglial Copper-Assisted Polyamine Metabolism during Intense Network Activity JF - BIOMOLECULES J2 - BIOMOLECULES VL - 11 PY - 2021 IS - 4 SN - 2218-273X DO - 10.3390/biom11040604 UR - https://m2.mtmt.hu/api/publication/31971626 ID - 31971626 N1 - Functional Pharmacology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Budapest, H-1117, Hungary Hevesy György Ph.D. School of Chemistry, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Export Date: 28 July 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.hu Funding details: Hungarian Scientific Research Fund, OTKA, K124558 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding: This research was partly supported by the grant OTKA K124558. László Héja is a recipient of the János Bolyai Scholarship of the Hungarian Academy of Sciences. Funding text 2: This research was partly supported by the grant OTKA K124558. L?szl? H?ja is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. Functional Pharmacology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Budapest, H-1117, Hungary Hevesy György Ph.D. School of Chemistry, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Export Date: 29 July 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.hu Funding details: Hungarian Scientific Research Fund, OTKA, K124558 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding: This research was partly supported by the grant OTKA K124558. László Héja is a recipient of the János Bolyai Scholarship of the Hungarian Academy of Sciences. Funding text 2: This research was partly supported by the grant OTKA K124558. L?szl? H?ja is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. Functional Pharmacology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Budapest, H-1117, Hungary Hevesy György Ph.D. School of Chemistry, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Export Date: 23 September 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.hu Chemicals/CAS: calcium ion, 14127-61-8; copper, 15158-11-9, 7440-50-8; glutamine, 56-85-9, 6899-04-3; silver nitrate, 7761-88-8 Funding details: Hungarian Scientific Research Fund, OTKA, K124558 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding: This research was partly supported by the grant OTKA K124558. László Héja is a recipient of the János Bolyai Scholarship of the Hungarian Academy of Sciences. Funding text 2: This research was partly supported by the grant OTKA K124558. L?szl? H?ja is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. Functional Pharmacology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Budapest, H-1117, Hungary Hevesy György Ph.D. School of Chemistry, ELTE Eötvös Loránd University, Budapest, H-1117, Hungary Export Date: 24 September 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.hu Chemicals/CAS: calcium ion, 14127-61-8; copper, 15158-11-9, 7440-50-8; glutamine, 56-85-9, 6899-04-3; silver nitrate, 7761-88-8 Funding details: Hungarian Scientific Research Fund, OTKA, K124558 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: Funding: This research was partly supported by the grant OTKA K124558. László Héja is a recipient of the János Bolyai Scholarship of the Hungarian Academy of Sciences. Funding text 2: This research was partly supported by the grant OTKA K124558. L?szl? H?ja is a recipient of the J?nos Bolyai Scholarship of the Hungarian Academy of Sciences. LA - English DB - MTMT ER - TY - JOUR AU - Héja, László AU - Kardos, Julianna TI - NCX activity generates spontaneous Ca2+ oscillations in the astrocytic leaflet microdomain JF - CELL CALCIUM J2 - CELL CALCIUM VL - 86 PY - 2020 IS - March 2020 SN - 0143-4160 DO - 10.1016/j.ceca.2019.102137 UR - https://m2.mtmt.hu/api/publication/31123982 ID - 31123982 N1 - Export Date: 13 January 2020 CODEN: CECAD Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of SciencesHungary; email: heja.laszlo@ttk.mta.hu Chemicals/CAS: calcium ion, 14127-61-8; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; sodium ion, 17341-25-2 Funding details: K124558 . Funding text 1: This work was supported by grants VEKOP - 2.1.1-15-2016-00156 and National Research, Development and Innovation Office grant OTKA K124558 . Export Date: 23 February 2021 CODEN: CECAD Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Funding Agency and Grant Number: National Research, Development and Innovation Office [OTKA K124558]; [VEKOP-2.1.1-15-2016-00156] Funding text: This work was supported by grants VEKOP-2.1.1-15-2016-00156 and National Research, Development and Innovation Office grant OTKA K124558. Cited By :2 Export Date: 6 April 2021 CODEN: CECAD Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Cited By :2 Export Date: 7 April 2021 CODEN: CECAD Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Cited By :4 Export Date: 23 September 2021 CODEN: CECAD Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Chemicals/CAS: calcium ion, 14127-61-8; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; sodium ion, 17341-25-2; calcium, 7440-70-2, 14092-94-5; Calcium; Sodium-Calcium Exchanger Funding details: OTKA K124558 Funding text 1: This work was supported by grants VEKOP-2.1.1-15-2016-00156 and National Research, Development and Innovation Office grant OTKA K124558. AB - The synergy between synaptic Glu release and astrocytic Glu-Na+ symport is essential to the signalling function of the tripartite synapse. Here we used kinetic data of astrocytic Glu transporters (EAAT) and the Na+/Ca2+ exchanger (NCX) to simulate Glu release, Glu uptake and subsequent Na+ and Ca2+ dynamics in the astrocytic leaflet microdomain following single release event. Model simulations show that Glu-Na+ symport differently affect intracellular [Na+] in synapses with different extent of astrocytic coverage. Surprisingly, NCX activity alone has been shown to generate markedly stable, spontaneous Ca2+ oscillation in the astrocytic leaflet. These on-going oscillations appear when NCX operates either in the forward or reverse direction. We conjecture that intrinsic NCX activity may play a prominent role in the generation of astrocytic Ca2+ oscillations. © 2019 The Authors LA - English DB - MTMT ER - TY - JOUR AU - Simon, Ágnes AU - Magyar, Csaba AU - Héja, László AU - Kardos, Julianna TI - Peptide Binding Sites of Connexin Proteins JF - CHEMISTRY J2 - CHEMISTRY VL - 2 PY - 2020 IS - 3 SP - 662 EP - 673 PG - 12 SN - 2624-8549 DO - 10.3390/chemistry2030042 UR - https://m2.mtmt.hu/api/publication/31795296 ID - 31795296 LA - English DB - MTMT ER - TY - JOUR AU - Héja, László AU - Simon, Ágnes AU - Szabó, Zsolt AU - Kardos, Julianna TI - Feedback adaptation of synaptic excitability via Glu:Na+ symport driven astrocytic GABA and Gln release JF - NEUROPHARMACOLOGY J2 - NEUROPHARMACOLOGY VL - 161 PY - 2019 SN - 0028-3908 DO - 10.1016/j.neuropharm.2019.05.006 UR - https://m2.mtmt.hu/api/publication/30721430 ID - 30721430 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office Budapest, Hungary [VEKOP2.1.1-15-2016-00156, OTKA K124558] Funding text: This work was supported by grants of the National Research, Development and Innovation Office Budapest, Hungary grant VEKOP2.1.1-15-2016-00156 and grant OTKA K124558. Cited By :3 Export Date: 6 April 2021 CODEN: NEPHB Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Cited By :3 Export Date: 7 April 2021 CODEN: NEPHB Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Julianna AU - Dobolyi, Árpád AU - Szabó, Zsolt AU - Simon, Ágnes AU - Lourmet, Guillaume AU - Palkovits, Miklós AU - Héja, László TI - Molecular Plasticity of the Nucleus Accumbens Revisited—Astrocytic Waves Shall Rise JF - MOLECULAR NEUROBIOLOGY J2 - MOL NEUROBIOL VL - 56 PY - 2019 IS - 12 SP - 7950 EP - 7965 PG - 16 SN - 0893-7648 DO - 10.1007/s12035-019-1641-z UR - https://m2.mtmt.hu/api/publication/30694084 ID - 30694084 N1 - Funding Agency and Grant Number: MTA Research Centre for Natural Sciences (MTA TTK) [KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156, OTKA K124558] Funding text: Open access funding provided by MTA Research Centre for Natural Sciences (MTA TTK). This work was supported by grants KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156 and OTKA K124558. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Üllői út 26, Budapest, 1086, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Eötvös Loránd University and the Hungarian Academy of Sciences, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Human Brain Tissue Bank, Semmelweis University, Tűzoltó utca 58, Budapest, H-1094, Hungary Export Date: 10 January 2020 CODEN: MONBE Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Üllői út 26, Budapest, 1086, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Eötvös Loránd University and the Hungarian Academy of Sciences, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Human Brain Tissue Bank, Semmelweis University, Tűzoltó utca 58, Budapest, H-1094, Hungary Cited By :5 Export Date: 17 March 2021 CODEN: MONBE Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; Adenosine Triphosphate; gamma-Aminobutyric Acid; Glutamic Acid Funding details: K124558. Funding text 1: Open access funding provided by MTA Research Centre for Natural Sciences (MTA TTK). This work was supported by grants KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156 and OTKA K124558. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Üllői út 26, Budapest, 1086, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Eötvös Loránd University and the Hungarian Academy of Sciences, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Human Brain Tissue Bank, Semmelweis University, Tűzoltó utca 58, Budapest, H-1094, Hungary Cited By :5 Export Date: 6 April 2021 CODEN: MONBE Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Üllői út 26, Budapest, 1086, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Eötvös Loránd University and the Hungarian Academy of Sciences, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Human Brain Tissue Bank, Semmelweis University, Tűzoltó utca 58, Budapest, H-1094, Hungary Cited By :5 Export Date: 7 April 2021 CODEN: MONBE Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Laboratory of Neuromorphology, Department of Anatomy, Histology and Embryology, Semmelweis University, Üllői út 26, Budapest, 1086, Hungary MTA-ELTE Laboratory of Molecular and Systems Neurobiology, Department of Physiology and Neurobiology, Eötvös Loránd University and the Hungarian Academy of Sciences, Pázmány Péter sétány 1C, Budapest, 1117, Hungary Human Brain Tissue Bank, Semmelweis University, Tűzoltó utca 58, Budapest, H-1094, Hungary Cited By :7 Export Date: 28 July 2021 CODEN: MONBE Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; Adenosine Triphosphate; gamma-Aminobutyric Acid; Glutamic Acid Funding details: K124558. Funding text 1: Open access funding provided by MTA Research Centre for Natural Sciences (MTA TTK). This work was supported by grants KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156 and OTKA K124558. LA - English DB - MTMT ER - TY - JOUR AU - Vincze, R. AU - Péter, Márton AU - Szabó, Zsolt AU - Kardos, Julianna AU - Kovács, Zsolt AU - Héja, László TI - Astrocytic gap junctions differentially affect seizures in absence and temporal lobe epilepsy models JF - GLIA J2 - GLIA VL - 67 PY - 2019 SP - E710 EP - E711 PG - 2 SN - 0894-1491 UR - https://m2.mtmt.hu/api/publication/31096625 ID - 31096625 N1 - Poszter Funding Agency and Grant Number: National Research, Development and Innovation Office grant [OTKA K124558]; [VEKOP-2.1.1-15-2016-00156] Funding text: This work was supported by grants VEKOP-2.1.1-15-2016-00156 and National Research, Development and Innovation Office grant OTKA K124558. Supplement: S1 LA - English DB - MTMT ER - TY - JOUR AU - Vincze, Renáta AU - Péter, Márton AU - Szabó, Zsolt AU - Kardos, Julianna AU - Héja, László AU - Kovács, Zsolt TI - Connexin 43 Differentially Regulates Epileptiform Activity in Models of Convulsive and Non-Convulsive Epilepsies JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 13 PY - 2019 SN - 1662-5102 DO - 10.3389/fncel.2019.00173 UR - https://m2.mtmt.hu/api/publication/30685187 ID - 30685187 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [OTKA K124558]; [VEKOP-2.1.1-15-2016-00156] Funding text: This work was supported by grants VEKOP-2.1.1-15-2016-00156 and National Research, Development and Innovation Office grant no. OTKA K124558. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Department of Biology, Eötvös Loránd University, Savaria University Centre, Szombathely, Hungary Cited By :5 Export Date: 6 April 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Department of Biology, Eötvös Loránd University, Savaria University Centre, Szombathely, Hungary Cited By :5 Export Date: 7 April 2021 Correspondence Address: Héja, L.; Functional Pharmacology Research Group, Hungary; email: heja.laszlo@ttk.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Julianna AU - Héja, László AU - Simon, Ágnes AU - Jablonkai, István AU - Kovacs, Richard AU - Jemnitz, Katalin TI - Copper signalling: causes and consequences (vol 16, 71 , 2018) JF - CELL COMMUNICATION AND SIGNALING J2 - CELL COMM SIGN VL - 16 PY - 2018 PG - 3 SN - 1478-811X DO - 10.1186/s12964-018-0292-4 UR - https://m2.mtmt.hu/api/publication/30379283 ID - 30379283 AB - Following publication of the original article [1], the authors reported an error in Table 3. The correct version of Table 3 is shown below:The publishers apologise for this error. The original article [1] has been corrected. LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Julianna AU - Héja, László AU - Simon, Ágnes AU - Jablonkai, István AU - Kovács, Richárd AU - Jemnitz, Katalin TI - Copper signalling: causes and consequences JF - CELL COMMUNICATION AND SIGNALING J2 - CELL COMM SIGN VL - 16 PY - 2018 IS - 1 PG - 21 SN - 1478-811X DO - 10.1186/s12964-018-0277-3 UR - https://m2.mtmt.hu/api/publication/30317831 ID - 30317831 N1 - Journal Article; Review Funding Agency and Grant Number: [KMR_12-1-2012-0112 TRANSRAT]; [VEKOP-2.1.1-15-2016-00156]; [OTKA K124558] Funding text: This work was supported by grants KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156 and OTKA K124558. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, Budapest, 1117, Hungary Institute of Neurophysiology, Charité-Universitätsmedizin, Berlin, Germany Cited By :28 Export Date: 6 April 2021 Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar Tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, Budapest, 1117, Hungary Institute of Neurophysiology, Charité-Universitätsmedizin, Berlin, Germany Cited By :28 Export Date: 7 April 2021 Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar Tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, Budapest, 1117, Hungary Institute of Neurophysiology, Charité-Universitätsmedizin, Berlin, Germany Cited By :37 Export Date: 29 July 2021 Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Magyar Tudósok körútja 2, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; captopril, 62571-86-2; clioquinol, 130-26-7, 8057-20-3; copper, 15158-11-9, 7440-50-8; disulfiram, 97-77-8; elesclomol, 488832-69-5; glutathione, 70-18-8; metformin, 1115-70-4, 657-24-9; penicillamine, 2219-30-9, 52-67-5; tetrathiomolybdic acid, 13718-35-9, 16330-92-0; trientine, 112-24-3, 38260-01-4; Copper Funding details: Hungarian Scientific Research Fund, OTKA, K124558 Funding text 1: This work was supported by grants KMR_12-1-2012-0112 TRANSRAT, VEKOP-2.1.1-15-2016-00156 and OTKA K124558. AB - Copper-containing enzymes perform fundamental functions by activating dioxygen (O-2) and therefore allowing chemical energy-transfer for aerobic metabolism. The copper-dependence of O-2 transport, metabolism and production of signalling molecules are supported by molecular systems that regulate and preserve tightly-bound static and weakly-bound dynamic cellular copper pools. Disruption of the reducing intracellular environment, characterized by glutathione shortage and ambient Cu(II) abundance drives oxidative stress and interferes with the bidirectional, copper-dependent communication between neurons and astrocytes, eventually leading to various brain disease forms. A deeper understanding of of the regulatory effects of copper on neuro-glia coupling via polyamine metabolism may reveal novel copper signalling functions and new directions for therapeutic intervention in brain disorders associated with aberrant copper metabolism. LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Julianna AU - Héja, László AU - Jemnitz, Katalin AU - Kovacs, R AU - Palkovits, Miklós TI - The nature of early astroglial protection-Fast activation and signaling.. Fast activation and signaling TS - Fast activation and signaling JF - PROGRESS IN NEUROBIOLOGY: AN INTERNATIONAL REVIEW JOURNAL J2 - PROG NEUROBIOL VL - 153 PY - 2017 SP - 86 EP - 99 PG - 14 SN - 0301-0082 DO - 10.1016/j.pneurobio.2017.03.005 UR - https://m2.mtmt.hu/api/publication/3209058 ID - 3209058 N1 - Journal Article; Review; Research Support, Non-U.S. Gov't --- összevonáskor átvett szöveg --- doi: 10.1016/j.pneurobio.2017.03.005. [Epub ahead of print] Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :4 Export Date: 22 July 2019 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :8 Export Date: 15 April 2020 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :8 Export Date: 22 April 2020 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :9 Export Date: 29 May 2020 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :12 Export Date: 31 July 2020 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 21 September 2020 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 17 March 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 29 March 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Funding Agency and Grant Number: ERA-Chemistry grant [OTKA102166]; [KMR_12-1-2012-0112] Funding text: We gratefully thank Dr Orsolya Toke for her critical comments and careful editing of the manuscript as well as professor Ferenc Hajos for the tripartite synapse photocopy shown in Fig. 3. Financial support by the KMR_12-1-2012-0112 and ERA-Chemistry OTKA102166 grants are greatly acknowledged. Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 6 April 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 7 April 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :13 Export Date: 20 April 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :14 Export Date: 5 May 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid Functional Pharmacology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Hungary Institute of Neurophysiology, Charité – Universitätsmedizin, Berlin, Germany Human Brain Tissue Bank and Laboratory, Semmelweis University, Budapest, Hungary Cited By :15 Export Date: 14 September 2021 CODEN: PGNBA Correspondence Address: Kardos, J.; Research Centre for Natural Sciences, H‐1117 Budapest, Hungary; email: kardos.julianna@ttk.mta.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; carrier protein, 80700-39-6; estradiol, 50-28-2; glutamate ammonia ligase, 9023-70-5; glutamate dehydrogenase, 9001-46-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; raloxifene, 82640-04-8, 84449-90-1; succinic acid, 110-15-6; tamoxifen, 10540-29-1; gamma-Aminobutyric Acid; Glutamic Acid; Succinic Acid AB - Our present review is focusing on the uniqueness of balanced astroglial signaling. The balance of excitatory and inhibitory signaling within the CNS is mainly determined by sharp synaptic transients of excitatory glutamate (Glu) and inhibitory gamma- aminobutyrate (GABA) acting on the sub-second timescale. Astroglia is involved in excitatory chemical transmission by taking up i) Glu through neurotransmitter-sodium transporters, ii) K+ released due to presynaptic action potential generation, and iii) water keeping osmotic pressure. Glu uptake-coupled Na+ influx may either ignite long-range astroglial Ca2+ transients or locally counteract over-excitation via astroglial GABA release and increased tonic inhibition. Imbalance of excitatory and inhibitory drives is associated with a number of disease conditions, including prevalent traumatic and ischaemic injuries or the emergence of epilepsy. Therefore, when addressing the potential of early therapeutic intervention, astroglial signaling functions combating progress of Glu excitotoxicity is of critical importance. We suggest, that excitotoxicity is linked primarily to over-excitation induced by the impairment of astroglial Glu uptake and/or GABA release. Within this framework, we discuss the acute alterations of Glu- cycling and metabolism and conjecture the therapeutic promise of regulation. We also confer the role played by key carrier proteins and enzymes as well as their interplay at the molecular, cellular, and organ levels. Moreover, based on our former studies, we offer potential prospect on the emerging theme of astroglial succinate sensing in course of Glu excitotoxicity. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Zsolt AU - Héja, László AU - Szalay, Gergely AU - Kékesi, Orsolya Sára AU - Füredi, András AU - Szebényi, Kornélia AU - Dobolyi, Árpád AU - Orbán, Tamás I. AU - Kolacsek, Orsolya AU - Tompa, Tamás AU - Miskolczy, Zsombor AU - Biczók, László AU - Rózsa J., Balázs AU - Sarkadi, Balázs AU - Kardos, Julianna TI - Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 7 PY - 2017 PG - 18 SN - 2045-2322 DO - 10.1038/s41598-017-06073-7 UR - https://m2.mtmt.hu/api/publication/3251043 ID - 3251043 AB - Slow wave activity (SWA) is a characteristic brain oscillation in sleep and quiet wakefulness. Although the cell types contributing to SWA genesis are not yet identified, the principal role of neurons in the emergence of this essential cognitive mechanism has not been questioned. To address the possibility of astrocytic involvement in SWA, we used a transgenic rat line expressing a calcium sensitive fluorescent protein in both astrocytes and interneurons and simultaneously imaged astrocytic and neuronal activity in vivo. Here we demonstrate, for the first time, that the astrocyte network display synchronized recurrent activity in vivo coupled to UP states measured by field recording and neuronal calcium imaging. Furthermore, we present evidence that extensive synchronization of the astrocytic network precedes the spatial build-up of neuronal synchronization. The earlier extensive recruitment of astrocytes in the synchronized activity is reinforced by the observation that neurons surrounded by active astrocytes are more likely to join SWA, suggesting causality. Further supporting this notion, we demonstrate that blockade of astrocytic gap junctional communication or inhibition of astrocytic Ca2+ transients reduces the ratio of both astrocytes and neurons involved in SWA. These in vivo findings conclusively suggest a causal role of the astrocytic syncytium in SWA generation. LA - English DB - MTMT ER - TY - JOUR AU - Kardos, Julianna AU - Szabó, Zsolt AU - Héja, László TI - Framing Neuro-Glia Coupling in Antiepileptic Drug Design. JF - JOURNAL OF MEDICINAL CHEMISTRY J2 - J MED CHEM VL - 59 PY - 2016 IS - 3 SP - 777 EP - 787 PG - 11 SN - 0022-2623 DO - 10.1021/acs.jmedchem.5b00331 UR - https://m2.mtmt.hu/api/publication/2972586 ID - 2972586 N1 - Funding Agency and Grant Number: TRANS-RAT [KMR_12-1-2012-0112]; ERA Chemistry [NN 102166] Funding text: Financial support from Grants KMR_12-1-2012-0112 TRANS-RAT and ERA Chemistry NN 102166 on the "Novel targets and new drug candidates to combat epilepsy: Design of subtype-selective spirobicyclic inhibitors to distinguish among gamma-aminobutyric acid transporter protein subtypes" is greatly acknowledged. Cited By :13 Export Date: 6 April 2021 CODEN: JMCMA Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Hungary; email: kardos.julianna@ttk.mta.hu Cited By :13 Export Date: 7 April 2021 CODEN: JMCMA Correspondence Address: Kardos, J.; Functional Pharmacology Research Group, Hungary; email: kardos.julianna@ttk.mta.hu AB - We delineate perspectives for the design and discovery of antiepileptic drugs (AEDs) with fewer side effects by focusing on astroglial modulation of spatiotemporal seizure dynamics. It is now recognized that the major inhibitory neurotransmitter of the brain, gamma-aminobutyric acid (GABA), can be released through the reversal of astroglial GABA transporters. Synaptic spillover and subsequent glutamate (Glu) uptake in neighboring astrocytes evoke replacement of extracellular Glu for GABA, driving neurons away from the seizure threshold. Attenuation of synaptic signaling by this negative feedback through the interplay of Glu and GABA transporters of adjacent astroglia can result in shortened seizures. By contrast, long-range activation of astroglia through gap junctions may promote recurrent seizures on the model of pharmacoresistant temporal lobe epilepsy. From their first detection to our current understanding, we identify various targets that shape both short- and long-range neuro-astroglia coupling, as these are manifest in epilepsy phenomena and in the associated research promotions of AED. LA - English DB - MTMT ER - TY - JOUR AU - Kirischuk, S AU - Héja, László AU - Kardos, Julianna AU - Billups, B TI - Astrocyte sodium signaling and the regulation of neurotransmission JF - GLIA J2 - GLIA VL - 64 PY - 2016 IS - 10 SP - 1655 EP - 1666 PG - 12 SN - 0894-1491 DO - 10.1002/glia.22943 UR - https://m2.mtmt.hu/api/publication/2972585 ID - 2972585 N1 - University Medical Center of the Johannes Gutenberg University Mainz, Institute of Physiology, Mainz, Germany Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, ACT, Australia Cited By :38 Export Date: 6 April 2021 CODEN: GLIAE Correspondence Address: Kirischuk, S.; University Medical Center of the Johannes Gutenberg University Mainz, Germany; email: brian.billups@anu.edu.au AB - The transmembrane Na+ concentration gradient is an important source of energy required not only to enable the generation of action potentials in excitable cells, but also for various transmembrane transporters both in excitable and non-excitable cells, like astrocytes. One of the vital functions of astrocytes in the central nervous system (CNS) is to regulate neurotransmitter concentrations in the extracellular space. Most neurotransmitters in the CNS are removed from the extracellular space by Na+ -dependent neurotransmitter transporters (NeuTs) expressed both in neurons and astrocytes. Neuronal NeuTs control mainly phasic synaptic transmission, i.e., synaptically induced transient postsynaptic potentials, while astrocytic NeuTs contribute to the termination of phasic neurotransmission and modulate the tonic tone, i.e., the long-lasting activation of extrasynaptic receptors by neurotransmitter that has diffused out of the synaptic cleft. Consequently, local intracellular Na+ ([Na+ ]i ) transients occurring in astrocytes, for example via the activation of ionotropic neurotransmitter receptors, can affect the driving force for neurotransmitter uptake, in turn modulating the spatio-temporal profiles of neurotransmitter levels in the extracellular space. As some NeuTs are close to thermodynamic equilibrium under resting conditions, an increase in astrocytic [Na+ ]i can stimulate the direct release of neurotransmitter via NeuT reversal. In this review we discuss the role of astrocytic [Na+ ]i changes in the regulation of uptake/release of neurotransmitters. It is emphasized that an activation of one neurotransmitter system, including either its ionotropic receptor or Na+ -coupled co-transporter, can strongly influence, or even reverse, other Na+ -dependent NeuTs, with potentially significant consequences for neuronal communication. GLIA 2015. LA - English DB - MTMT ER - TY - JOUR AU - Ma, X AU - Lubin, H AU - Ioja, Enikő AU - Kékesi, Orsolya Sára AU - Simon, Ágnes AU - Apáti, Ágota AU - Orbán, Tamás I. AU - Héja, László AU - Kardos, Julianna AU - Marko, IE TI - Straightforward and effective synthesis of gamma-aminobutyric acid transporter subtype 2-selective acyl-substituted azaspiro[4.5]decanes JF - BIOORGANIC & MEDICINAL CHEMISTRY LETTERS J2 - BIOORG MED CHEM LETT VL - 26 PY - 2016 IS - 2 SP - 417 EP - 423 PG - 7 SN - 0960-894X DO - 10.1016/j.bmcl.2015.11.100 UR - https://m2.mtmt.hu/api/publication/2988827 ID - 2988827 AB - Supply of major metabolites such as gamma-aminobutyric acid (GABA), beta-alanine and taurine is an essential instrument that shapes signalling, proper cell functioning and survival in the brain and peripheral organs. This background motivates the synthesis of novel classes of compounds regulating their selective transport through various fluid-organ barriers via the low-affinity gamma-aminobutyric acid (GABA) transporter subtype 2 (GAT2). Natural and synthetic spirocyclic compounds or therapeutics with a range of structures and biological activity are increasingly recognised in this regard. Based on pre-validated GABA transport activity, straightforward and efficient synthesis method was developed to provide an azaspiro[4.5]decane scaffold, holding a variety of charge, substituent and 3D constrain of spirocyclic amine. Investigation of the azaspiro[4.5]decane scaffold in cell lines expressing the four GABA transporter subtypes led to the discovery of a subclass of a GAT2-selective compounds with acyl-substituted azaspiro[4.5]decane core. LA - English DB - MTMT ER -