TY - JOUR AU - Basa, Péter AU - Fodor, B. AU - Nagy, Zs. AU - Oyunbolor, B. AU - Hajtman, A. AU - Bordács, Sándor AU - Kézsmárki, István AU - Halbritter, András Ernő AU - Orbánová, Agnesa TI - Analysis of malaria infection byproducts with Mueller matrix transmission ellipsometry JF - THIN SOLID FILMS J2 - THIN SOLID FILMS VL - 766 PY - 2023 SN - 0040-6090 DO - 10.1016/j.tsf.2022.139637 UR - https://m2.mtmt.hu/api/publication/33548398 ID - 33548398 AB - In this work, hemozoin, a microcrystalline byproduct of the malaria parasites was studied by transmission Mueller matrix ellipsometry. Measurement data was collected for different magnetic field orientations and as a function of the density of the hemozoin suspension. Our ellipsometric study demonstrates the magnetic alignment of the hemozoin crystals via the corresponding large linear birefringence and dichroism signals. These results reveal optical anisotropies of this material, which could be utilized for future optimization of detection schemes or optical instruments for diagnostic use. LA - English DB - MTMT ER - TY - JOUR AU - Arndt, L. AU - Koleala, T. AU - Orbánová, Agnesa AU - Ibam, C. AU - Lufele, E. AU - Timinao, L. AU - Lorry, L. AU - Butykai, Ádám AU - Kaman, P. AU - Molnár, Petra AU - Krohns, S. AU - Nate, E. AU - Kucsera, I. AU - Orosz, E. AU - Moore, B. AU - Robinson, L.J. AU - Laman, M. AU - Kézsmárki, István AU - Karl, S. TI - Magneto-optical diagnosis of symptomatic malaria in Papua New Guinea JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 SN - 2041-1723 DO - 10.1038/s41467-021-21110-w UR - https://m2.mtmt.hu/api/publication/31902046 ID - 31902046 N1 - Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 5 March 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: National Health and Medical Research Council, NHMRC, GNT1127356 Funding details: James Cook University, JCU Funding text 1: The authors would like to sincerely thank all study participants. We thank Yagaum Hospital and Madang Town Clinic staff for their collaboration in this research study. We would like to thank Professor William Pomat, Dr. Livingstone Tavul, the PNGIMR microscopy unit, and molecular parasitology and entomology laboratory staff for their support. We would like to thank Ivo Mueller and Louis Schofield for helpful discussions. S.Ka. (GNT1141441) and L.J.R. (GNT1161627) are recipients of an Australian National Health and Medical Research Council (NHMRC) Career Development Fellowship. This study was funded by NHMRC Project Grant (GNT1127356). L.T. is supported by a James Cook University PhD scholarship. A.B. and A.O. were supported by the BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT). Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 20 April 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 13 May 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Chemicals/CAS: hemozoin, 39404-00-7; Hemeproteins; hemozoin Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: National Health and Medical Research Council, NHMRC, GNT1127356 Funding details: James Cook University, JCU Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding text 1: The authors would like to sincerely thank all study participants. We thank Yagaum Hospital and Madang Town Clinic staff for their collaboration in this research study. We would like to thank Professor William Pomat, Dr. Livingstone Tavul, the PNGIMR microscopy unit, and molecular parasitology and entomology laboratory staff for their support. We would like to thank Ivo Mueller and Louis Schofield for helpful discussions. S.Ka. (GNT1141441) and L.J.R. (GNT1161627) are recipients of an Australian National Health and Medical Research Council (NHMRC) Career Development Fellowship. This study was funded by NHMRC Project Grant (GNT1127356). L.T. is supported by a James Cook University PhD scholarship. A.B. and A.O. were supported by the BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT). Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 14 May 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Chemicals/CAS: hemozoin, 39404-00-7; Hemeproteins; hemozoin Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: National Health and Medical Research Council, NHMRC, GNT1127356 Funding details: James Cook University, JCU Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding text 1: The authors would like to sincerely thank all study participants. We thank Yagaum Hospital and Madang Town Clinic staff for their collaboration in this research study. We would like to thank Professor William Pomat, Dr. Livingstone Tavul, the PNGIMR microscopy unit, and molecular parasitology and entomology laboratory staff for their support. We would like to thank Ivo Mueller and Louis Schofield for helpful discussions. S.Ka. (GNT1141441) and L.J.R. (GNT1161627) are recipients of an Australian National Health and Medical Research Council (NHMRC) Career Development Fellowship. This study was funded by NHMRC Project Grant (GNT1127356). L.T. is supported by a James Cook University PhD scholarship. A.B. and A.O. were supported by the BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT). Funding Agency and Grant Number: Australian National Health and Medical Research Council (NHMRC)National Health and Medical Research Council of Australia [GNT1141441, GNT1161627]; NHMRCNational Health and Medical Research Council of Australia [GNT1127356]; James Cook University; BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT) Funding text: The authors would like to sincerely thank all study participants. We thank Yagaum Hospital and Madang Town Clinic staff for their collaboration in this research study. We would like to thank Professor William Pomat, Dr. Livingstone Tavul, the PNGIMR microscopy unit, and molecular parasitology and entomology laboratory staff for their support. We would like to thank Ivo Mueller and Louis Schofield for helpful discussions. S.Ka. (GNT1141441) and L.J.R. (GNT1161627) are recipients of an Australian National Health and Medical Research Council (NHMRC) Career Development Fellowship. This study was funded by NHMRC Project Grant (GNT1127356). L.T. is supported by a James Cook University PhD scholarship. A.B. and A.O. were supported by the BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT). Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 27 May 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Cited By :4 Export Date: 8 September 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Institute of Natural Materials Technology, University of Technology, Dresden, Germany Vector-borne Diseases Unit, PNG Institute of Medical Research, Madang, Madang Province, Papua New Guinea Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia Experimental Physics 5, Center for Electronic Correlations and Magnetism, University of Augsburg, Augsburg, Germany National Public Health Center, Budapest, Hungary School of Pharmacy, Curtin University, Bentley, WA, Australia Burnet Institute, Melbourne, VIC, Australia Export Date: 18 September 2021 Correspondence Address: Karl, S.; Vector-borne Diseases Unit, Papua New Guinea; email: stephan.karl@jcu.edu.au Correspondence Address: Kézsmárki, I.; Department of Physics, Hungary; email: istvan.kezsmarki@physik.uni-augsburg.de Chemicals/CAS: hemozoin, 39404-00-7; Hemeproteins; hemozoin Tradenames: CFX96 Touch, Biorad, Australia Manufacturers: Biorad, Australia Funding details: Budapesti Műszaki és Gazdaságtudományi Egyetem, BME Funding details: National Health and Medical Research Council, NHMRC, GNT1127356 Funding details: James Cook University, JCU Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding text 1: The authors would like to sincerely thank all study participants. We thank Yagaum Hospital and Madang Town Clinic staff for their collaboration in this research study. We would like to thank Professor William Pomat, Dr. Livingstone Tavul, the PNGIMR microscopy unit, and molecular parasitology and entomology laboratory staff for their support. We would like to thank Ivo Mueller and Louis Schofield for helpful discussions. S.Ka. (GNT1141441) and L.J.R. (GNT1161627) are recipients of an Australian National Health and Medical Research Council (NHMRC) Career Development Fellowship. This study was funded by NHMRC Project Grant (GNT1127356). L.T. is supported by a James Cook University PhD scholarship. A.B. and A.O. were supported by the BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT). AB - Improved methods for malaria diagnosis are urgently needed. Here, we evaluate a novel method named rotating-crystal magneto-optical detection (RMOD) in 956 suspected malaria patients in Papua New Guinea. RMOD tests can be conducted within minutes and at low cost. We systematically evaluate the capability of RMOD to detect infections by directly comparing it with expert light microscopy, rapid diagnostic tests and polymerase chain reaction on capillary blood samples. We show that compared to light microscopy, RMOD exhibits 82% sensitivity and 84% specificity to detect any malaria infection and 87% sensitivity and 88% specificity to detect Plasmodium vivax. This indicates that RMOD could be useful in P. vivax dominated elimination settings. Parasite density correlates well with the quantitative magneto-optical signal. Importantly, residual hemozoin present in malaria-negative patients is also detectable by RMOD, indicating its ability to detect previous infections. This could be exploited to reveal transmission hotspots in low-transmission settings. © 2021, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Petra AU - Orbánová, Agnesa AU - Izrael, Richard AU - Babai, R. AU - Marton, L. AU - Butykai, Ádám AU - Karl, S. AU - Vértessy, Beáta (Grolmuszné) AU - Kézsmárki, István TI - Rapid and quantitative antimalarial drug efficacy testing via the magneto-optical detection of hemozoin JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 10 PY - 2020 IS - 1 PG - 11 SN - 2045-2322 DO - 10.1038/s41598-020-70860-y UR - https://m2.mtmt.hu/api/publication/31607172 ID - 31607172 N1 - Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Export Date: 22 September 2020 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of AugsburgHungary; email: molnar.petra@ttk.mta.hu Funding details: 2017-1.3.1-VKE-2017-00002, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, K119493, 2017-1.3.1-VKE-2017-00013 Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Export Date: 30 September 2020 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of AugsburgHungary; email: molnar.petra@ttk.mta.hu Funding details: 2017-1.3.1-VKE-2017-00002, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, K119493, 2017-1.3.1-VKE-2017-00013 Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Export Date: 7 January 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of AugsburgHungary; email: molnar.petra@ttk.mta.hu Funding details: 2017-1.3.1-VKE-2017-00002, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, K119493, 2017-1.3.1-VKE-2017-00013 Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Export Date: 10 February 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Funding details: 2017-1.3.1-VKE-2017-00002, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, K119493, 2017-1.3.1-VKE-2017-00013 Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, VEKOP-2.3.2-16-2017-00013]; NHMRC Career Development FellowshipNational Health and Medical Research Council of Australia [GNT 1141441]; BME-Biotechnology and Nanotechnology FIKP Grant (BME FIKPBIO); BME-Biotechnology and Nanotechnology FIKP Grant (FIKP-NAT) Funding text: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKPBIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Export Date: 25 February 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Funding details: 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, NKP-2018-1.2.1-NKP-2018-00005, VEKOP-2.3.2-16-2017-00013 Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI;NKFIH, K119493 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Cited By :1 Export Date: 13 May 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, K119493, NKP-2018-1.2.1-NKP-2018-00005, VEKOP-2.3.2-16-2017-00013 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Cited By :1 Export Date: 14 May 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, K119493, NKP-2018-1.2.1-NKP-2018-00005, VEKOP-2.3.2-16-2017-00013 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, NKP-2018-1.2.1-NKP-2018-00005]; BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO); BME-Biotechnology and Nanotechnology FIKP Grants (FIKP-NAT); NHMRC Career Development FellowshipNational Health and Medical Research Council of Australia [GNT 1141441] Funding text: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Cited By :1 Export Date: 27 May 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Cited By :1 Export Date: 8 September 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Malaria Research Laboratory, Institute of Enzymology, Research Centre for Natural Sciences, Budapest, 1117, Hungary Department of Physics, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, 6720, Hungary Department of Applied Biotechnology and Food Sciences, BME Budapest University of Technology and Economics, Budapest, 1111, Hungary Australian Institute of Tropical Health and Medicine, James Cook University, 1/14-88 McGregor Road, Smithfield, QLD 4870, Australia Vector-Borne Diseases Unit, PNG Institute of Medical Research, P.O. Box 378, Madang, Madang Province 511, Papua New Guinea Experimental Physics 5, Center for Electronic Correlations and Magnetism, Institute of Physics, University of Augsburg, Augsburg, 86159, Germany Cited By :1 Export Date: 18 September 2021 Correspondence Address: Molnár, P.; Department of Applied Biotechnology and Food Sciences, Hungary; email: molnar.petra@ttk.mta.hu Correspondence Address: Vértessy, B.G.; Department of Applied Biotechnology and Food Sciences, Hungary; email: vertessy@mail.bme.hu Correspondence Address: Kézsmárki, I.; Experimental Physics 5, Germany; email: istvan.kezsmarki@physik.uni-augsburg.de Chemicals/CAS: hemozoin, 39404-00-7; Antimalarials; Hemeproteins; hemozoin Funding details: National Health and Medical Research Council, NHMRC, GNT 1141441 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, K119493, NKP-2018-1.2.1-NKP-2018-00005, VEKOP-2.3.2-16-2017-00013 Funding text 1: The research reported in this paper was supported by the National Research, Development and Innovation Office of Hungary (K119493, 2017-1.3.1-VKE-2017-00002, 2017-1.3.1-VKE-2017-00013, VEKOP-2.3.2-16-2017-00013, NKP-2018-1.2.1-NKP-2018-00005), and the BME-Biotechnology and Nanotechnology FIKP Grants (BME FIKP-BIO and FIKP-NAT). SK was supported by an NHMRC Career Development Fellowship (GNT 1141441). AB - Emergence of resistant Plasmodium species makes drug efficacy testing a crucial part of malaria control. Here we describe a novel assay for sensitive, fast and simple drug screening via the magneto-optical detection of hemozoin, a natural biomarker formed during the hemoglobin metabolism of Plasmodium species. By quantifying hemozoin production over the intraerythrocytic cycle, we reveal that hemozoin formation is already initiated by ~ 6–12 h old ring-stage parasites. We demonstrate that the new assay is capable of drug efficacy testing with incubation times as short as 6–10 h, using synchronized P. falciparum 3D7 cultures incubated with chloroquine, piperaquine and dihydroartemisinin. The determined 50% inhibitory concentrations agree well with values established by standard assays requiring significantly longer testing time. Accordingly, we conclude that magneto-optical hemozoin detection provides a practical approach for the quick assessment of drug effect with short incubation times, which may also facilitate stage-specific assessment of drug inhibitory effects. © 2020, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Orbánová, Agnesa AU - Maria, Rebelo AU - Molnár, Petra AU - Inês, S Albuquerque AU - Butykai, Ádám AU - Kézsmárki, István TI - Efficient monitoring of blood-stage infection in a malaria rodent model by the rotating-crystal magneto-optical method JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 6 PY - 2016 PG - 9 SN - 2045-2322 DO - 10.1038/srep23218 UR - https://m2.mtmt.hu/api/publication/2902961 ID - 2902961 N1 - Funding Agency and Grant Number: Hungarian Research Funds OTKA [K108918] Funding text: We thank Thomas Hanscheid for the continuous support of the project and critical reading of the manuscript. We thank Carolina Tempera and Vanessa Zuzarte-Luis for their kind help during the experiments. This work was supported by the Hungarian Research Funds OTKA #K108918. Department of Physics, Budapest University of Technology and Economics, Budapest, 1111, Hungary Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, 1649-028, Portugal Cited By :5 Export Date: 26 September 2019 Correspondence Address: Orban, A.; Department of Physics, Budapest University of Technology and EconomicsHungary; email: orban@dept.phy.bme.hu Department of Physics, Budapest University of Technology and Economics, Budapest, 1111, Hungary Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, 1649-028, Portugal Cited By :10 Export Date: 10 February 2021 Correspondence Address: Orban, A.; Department of Physics, Hungary; email: orban@dept.phy.bme.hu Chemicals/CAS: hemozoin, 39404-00-7; DNA, Protozoan; Hemeproteins; hemozoin; Protozoan Proteins Funding details: Hungarian Scientific Research Fund, OTKA, 108918 Funding text 1: We thank Thomas Hänscheid for the continuous support of the project and critical reading of the manuscript. We thank Carolina Tempera and Vanessa Zuzarte-Luis for their kind help during the experiments. This work was supported by the Hungarian Research Funds OTKA #K108918. Funding Agency and Grant Number: Hungarian Research Funds OTKAOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K108918] Funding text: We thank Thomas Hanscheid for the continuous support of the project and critical reading of the manuscript. We thank Carolina Tempera and Vanessa Zuzarte-Luis for their kind help during the experiments. This work was supported by the Hungarian Research Funds OTKA #K108918. Department of Physics, Budapest University of Technology and Economics, Budapest, 1111, Hungary Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, 1649-028, Portugal Cited By :10 Export Date: 25 February 2021 Correspondence Address: Orban, A.; Department of Physics, Hungary; email: orban@dept.phy.bme.hu Chemicals/CAS: hemozoin, 39404-00-7; DNA, Protozoan; Hemeproteins; hemozoin; Protozoan Proteins Funding details: Hungarian Scientific Research Fund, OTKA, 108918 Funding text 1: We thank Thomas Hänscheid for the continuous support of the project and critical reading of the manuscript. We thank Carolina Tempera and Vanessa Zuzarte-Luis for their kind help during the experiments. This work was supported by the Hungarian Research Funds OTKA #K108918. Department of Physics, Budapest University of Technology and Economics, Budapest, 1111, Hungary Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, 1649-028, Portugal Cited By :11 Export Date: 13 May 2021 Correspondence Address: Orban, A.; Department of Physics, Hungary; email: orban@dept.phy.bme.hu Chemicals/CAS: hemozoin, 39404-00-7; DNA, Protozoan; Hemeproteins; hemozoin; Protozoan Proteins Funding details: Hungarian Scientific Research Fund, OTKA, 108918 Funding text 1: We thank Thomas Hänscheid for the continuous support of the project and critical reading of the manuscript. We thank Carolina Tempera and Vanessa Zuzarte-Luis for their kind help during the experiments. This work was supported by the Hungarian Research Funds OTKA #K108918. Department of Physics, Budapest University of Technology and Economics, Budapest, 1111, Hungary Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, 1649-028, Portugal Cited By :12 Export Date: 27 May 2021 Correspondence Address: Orban, A.; Department of Physics, Hungary; email: orban@dept.phy.bme.hu LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Recent advances in hemozoin-based malaria detection using the rotating-crystal magneto-optical method PY - 2016 UR - https://m2.mtmt.hu/api/publication/3089591 ID - 3089591 LA - English DB - MTMT ER - TY - JOUR AU - Pukáncsik, Mária AU - Orbánová, Agnesa AU - Nagy, Kinga AU - Matsuo, K AU - Gekko, K AU - Maurin, D AU - Hart, D AU - Kézsmárki, István AU - Vértessy, Beáta (Grolmuszné) TI - Secondary Structure Prediction of Protein Constructs Using Random Incremental Truncation and Vacuum-Ultraviolet CD Spectroscopy JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 6 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0156238 UR - https://m2.mtmt.hu/api/publication/3080797 ID - 3080797 N1 - Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Department of Physics, Budapest University of Technology and Economics, MTA-BME Lendület Magneto-optical Spectroscopy Research Group, Budapest, 1111, Hungary Hiroshima Synchrotron Radiation Center, Hiroshima University, Higashi, Hiroshima, Japan Institut de Biologie Structurale (IBS), CEA, CNRS, University Grenoble Alpes, Grenoble, 38044, France Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary Cited By :4 Export Date: 6 May 2021 CODEN: POLNC Chemicals/CAS: DNA-Binding Proteins; Drosophila Proteins; UDE protein, Drosophila Funding details: Japan Society for the Promotion of Science, JSPS, 15K07028 Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Department of Physics, Budapest University of Technology and Economics, MTA-BME Lendület Magneto-optical Spectroscopy Research Group, Budapest, 1111, Hungary Hiroshima Synchrotron Radiation Center, Hiroshima University, Higashi, Hiroshima, Japan Institut de Biologie Structurale (IBS), CEA, CNRS, University Grenoble Alpes, Grenoble, 38044, France Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary Cited By :4 Export Date: 12 May 2021 CODEN: POLNC Chemicals/CAS: DNA-Binding Proteins; Drosophila Proteins; UDE protein, Drosophila Funding details: Japan Society for the Promotion of Science, JSPS, 15K07028 Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [OTKA K 109486, 84008, OTKA K 108918]; FRISBI [ANR-10-INSB-05-1102]; GRAL within the Grenoble Partnership for Structural Biology (PSB) [ANR-10-LABX-49-01]; P-CUBE [INFRA-2008-227764]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [15K07028] Funding Source: KAKEN Funding text: National Research, Development and Innovation Office, Hungary (OTKA K 109486 and 84008 to BGV, OTKA K 108918 to IK).; The ESPRIT platform is part of the Grenoble Instruct centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI (ANR-10-INSB-05-1102) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB) and funded access was provided by the P-CUBE (INFRA-2008-227764). AB - A novel uracil-DNA degrading protein factor (termed UDE) was identified in Drosophila melanogaster with no significant structural and functional homology to other uracil-DNA binding or processing factors. Determination of the 3D structure of UDE is excepted to provide key information on the description of the molecular mechanism of action of UDE catalysis, as well as in general uracil-recognition and nuclease action. Towards this long-term aim, the random library ESPRIT technology was applied to the novel protein UDE to overcome problems in identifying soluble expressing constructs given the absence of precise information on domain content and arrangement. Nine constructs of UDE were chosen to decipher structural and functional relationships. Vacuum ultraviolet circular dichroism (VUVCD) spectroscopy was performed to define the secondary structure content and location within UDE and its truncated variants. The quantitative analysis demonstrated exclusive alpha-helical content for the full-length protein, which is preserved in the truncated constructs. Arrangement of alpha-helical bundles within the truncated protein segments suggested new domain boundaries which differ from the conserved motifs determined by sequence-based alignment of UDE homologues. Here we demonstrate that the combination of ESPRIT and VUVCD spectroscopy provides a new structural description of UDE and confirms that the truncated constructs are useful for further detailed functional studies. LA - English DB - MTMT ER - TY - JOUR AU - Rebelo, Maria AU - Grenho, Rita AU - Orbánová, Agnesa AU - Haenscheid, Thomas TI - Transdermal Diagnosis of Malaria Using Vapor Nanobubbles JF - EMERGING INFECTIOUS DISEASES J2 - EMERG INFECT DIS VL - 22 PY - 2016 IS - 2 SP - 343 EP - 344 PG - 2 SN - 1080-6040 DO - 10.3201/eid2202.151203 UR - https://m2.mtmt.hu/api/publication/30484135 ID - 30484135 N1 - Instituto de Medicina Molecular, Lisbon, Portugal Budapest University of Technology and Economics, Budapest, Hungary MTA-BME Lendulet Magneto-optical Spectroscopy Research Group, Budapest, Hungary CODEN: EIDIF Correspondence Address: Hänscheid, T.; Instituto de Medicina Molecular, Av Prof Egas Moniz, Portugal; email: t.hanscheid@medicina.ulisboa.pt LA - English DB - MTMT ER - TY - JOUR AU - Orbánová, Agnesa AU - Butykai, Ádám AU - Molnar, A AU - Prohle, Z AU - Fülöp, Gergő AU - Zelles, Tivadar AU - Forsyth, W AU - Hill, D AU - Muller, I AU - Schofield, L AU - Rebelo, M AU - Hanscheid, T AU - Karl, S AU - Kézsmárki, István TI - Evaluation of a novel magneto-optical method for the detection of malaria parasites JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 5 PG - 8 SN - 1932-6203 DO - 10.1371/journal.pone.0096981 UR - https://m2.mtmt.hu/api/publication/2710385 ID - 2710385 N1 - Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary Department of Oral Biology, Semmelweis University, Budapest, Hungary Infection and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia Queensland Tropical Health Alliance Australian Institute of Tropical Health and Medicine, James Cook University, Douglas, QLD, Australia Instituto de Medicina Molecular, Faculdade de Medicina, Lisbon, Portugal Condensed Matter Research Group, Hungarian Academy of Sciences, Budapest, Hungary CODEN: POLNC Chemicals/CAS: hemozoin, 39404-00-7; Biological Markers; Hemeproteins; hemozoin Funding details: GNT1021544, GNT1043345, GNT1052760, GNT1058665, GNT637406 Funding Agency and Grant Number: Hungarian Research FundsOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [OTKA K108918, TAMOP-4.2.1.B-09/1/KMR-2010-0001]; NHMRCNational Health and Medical Research Council of Australia [GNT1021544, GNT1043345, GNT637406, GNT1058665]; NHMRC early career research fellowshipNational Health and Medical Research Council of Australia [GNT1052760] Funding text: This work was supported by Hungarian Research Funds OTKA K108918, TAMOP-4.2.1.B-09/1/KMR-2010-0001, by NHMRC grants GNT1021544 and GNT1043345 awarded to Ivo Muller and by NHMRC grants GNT637406 and GNT1058665 awarded to Louis Schofield. Stephan Karl is supported through an NHMRC early career research fellowship (GNT1052760). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AB - Improving the efficiency of malaria diagnosis is one of the main goals of current malaria research. We have recently developed a magneto-optical (MO) method which allows high-sensitivity detection of malaria pigment (hemozoin crystals) in blood via the magnetically induced rotational motion of the hemozoin crystals. Here, we evaluate this MO technique for the detection of Plasmodium falciparum in infected erythrocytes using in-vitro parasite cultures covering the entire intraerythrocytic life cycle. Our novel method detected parasite densities as low as approximately 40 parasites per microliter of blood (0.0008% parasitemia) at the ring stage and less than 10 parasites/microL (0.0002% parasitemia) in the case of the later stages. These limits of detection, corresponding to approximately 20 pg/microL of hemozoin produced by the parasites, exceed that of rapid diagnostic tests and compete with the threshold achievable by light microscopic observation of blood smears. The MO diagnosis requires no special training of the operator or specific reagents for parasite detection, except for an inexpensive lysis solution to release intracellular hemozoin. The devices can be designed to a portable format for clinical and in-field tests. Besides testing its diagnostic performance, we also applied the MO technique to investigate the change in hemozoin concentration during parasite maturation. Our preliminary data indicate that this method may offer an efficient tool to determine the amount of hemozoin produced by the different parasite stages in synchronized cultures. Hence, it could eventually be used for testing the susceptibility of parasites to antimalarial drugs. LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Evaluation of a novel magneto-optical method for the detection of malaria PY - 2014 UR - https://m2.mtmt.hu/api/publication/2913605 ID - 2913605 LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Mesmerizing malaria PY - 2014 UR - https://m2.mtmt.hu/api/publication/2913598 ID - 2913598 LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Evaluation of the rotating-crystal magneto-optical method for the detection of malaria parasites PY - 2014 UR - https://m2.mtmt.hu/api/publication/2913588 ID - 2913588 LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa TI - Evaluation of a novel magneto-optical method for the detection of malaria parasites PY - 2014 UR - https://m2.mtmt.hu/api/publication/2913575 ID - 2913575 LA - English DB - MTMT ER - TY - JOUR AU - Butykai, Ádám AU - Orbánová, Agnesa AU - Kocsis, Vilmos AU - Szaller, Dávid AU - Bordács, Sándor AU - Tátrainé Szekeres, Erzsébet AU - Kiss, László Ferenc AU - Bóta, Attila AU - Vértessy, Beáta (Grolmuszné) AU - Zelles, Tivadar AU - Kézsmárki, István TI - Malaria pigment crystals as magnetic micro-rotors: Key for high-sensitivity diagnosis JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 3 PY - 2013 PG - 10 SN - 2045-2322 DO - 10.1038/srep01431 UR - https://m2.mtmt.hu/api/publication/2242098 ID - 2242098 AB - The need to develop new methods for the high-sensitivity diagnosis of malaria has initiated a global activity in medical and interdisciplinary sciences. Most of the diverse variety of emerging techniques are based on research-grade instruments, sophisticated reagent-based assays or rely on expertise. Here, we suggest an alternative optical methodology with an easy-to- use and cost-effective instrumentation based on unique properties of malaria pigment reported previously and determined quantitatively in the present study. Malaria pigment, also called hemozoin, is an insoluble microcrystalline form of heme. These crystallites show remarkable magnetic and optical anisotropy distinctly from any other components of blood. As a consequence, they can simultaneously act as magnetically driven micro-rotors and spinning polarizers in suspensions. These properties can gain importance not only in malaria diagnosis and therapies, where hemozoin is considered as drug target or immune modulator, but also in the magnetic manipulation of cells and tissues on the microscopic scale. LA - English DB - MTMT ER - TY - JOUR AU - Ceolin, G AU - Orbánová, Agnesa AU - Kocsis, Vilmos AU - Gyurcsányi, Ervin Róbert AU - Kézsmárki, István AU - Horváth, Viola TI - Electrochemical template synthesis of protein-imprinted magnetic polymer microrods JF - JOURNAL OF MATERIALS SCIENCE J2 - J MATER SCI VL - 48 PY - 2013 IS - 15 SP - 5209 EP - 5218 PG - 10 SN - 0022-2461 DO - 10.1007/s10853-013-7309-6 UR - https://m2.mtmt.hu/api/publication/2415941 ID - 2415941 N1 - Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest 1111, Hungary Department of Physics, Budapest University of Technology and Economics, Condensed Matter Research Group of the Hungarian Academy of Sciences, Budafoki út 8., Budapest 1111, Hungary MTA-BME Research Group of Technical Analytical Chemistry, Hungarian Academy of Sciences, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest 1111, Hungary Cited By :19 Export Date: 25 March 2021 CODEN: JMTSA Correspondence Address: Horváth, V.; MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4., Budapest 1111, Hungary; email: vhorvath@mail.bme.hu Funding details: European Commission, EC, MRTN-CT-2006-033873 Funding details: Hungarian Scientific Research Fund, OTKA, CNK80991, K104724, PD75615 Funding text 1: Acknowledgements This work is connected to the scientific program of the ‘‘Development of quality-oriented and harmonized R ? D ? I strategy and functional model at BME’’ project (TÁMOP-4.2.1/B-09/1/KMR-2010–0002). This work was supported by the European Commission (MRTN-CT-2006-033873) and Hungarian OTKA under Grant Nos. PD75615, K104724, and CNK80991. The authors would like to thank Dr. László Ferenc Kiss for the assistance during the magnetization measurements and Prof. László Bezúr for the atomic absorption measurements. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest 1111, Hungary Department of Physics, Budapest University of Technology and Economics, Condensed Matter Research Group of the Hungarian Academy of Sciences, Budafoki út 8., Budapest 1111, Hungary MTA-BME Research Group of Technical Analytical Chemistry, Hungarian Academy of Sciences, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest 1111, Hungary Cited By :19 Export Date: 27 March 2021 CODEN: JMTSA Correspondence Address: Horváth, V.; MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4., Budapest 1111, Hungary; email: vhorvath@mail.bme.hu Funding details: European Commission, EC, MRTN-CT-2006-033873 Funding details: Hungarian Scientific Research Fund, OTKA, CNK80991, K104724, PD75615 Funding text 1: Acknowledgements This work is connected to the scientific program of the ‘‘Development of quality-oriented and harmonized R ? D ? I strategy and functional model at BME’’ project (TÁMOP-4.2.1/B-09/1/KMR-2010–0002). This work was supported by the European Commission (MRTN-CT-2006-033873) and Hungarian OTKA under Grant Nos. PD75615, K104724, and CNK80991. The authors would like to thank Dr. László Ferenc Kiss for the assistance during the magnetization measurements and Prof. László Bezúr for the atomic absorption measurements. AB - A novel method for the electrochemical template synthesis of surface-imprinted magnetic polymer microrods for protein recognition is proposed. The polymer was electrodeposited into sacrificial cylindrical microreactors, the internal walls of which were previously modified with a target model protein, avidin, by simple physisorption. The electropolymerization was performed from a mixture of 3,4-ethylenedioxythiophene, poly(styrenesulfonate) (PSS), and PSS-coated superparamagnetic nanoparticles resulting in the formation of inherently electroconductive polymers confined to the volume of the microreactor. Here we show that: (i) the template synthesis within cylindrical microreactors results in polymer rods with dimensions matching that of the reactor, (ii) the incorporation of superparamagnetic particles induces magnetic properties that allow for efficient collection and manipulation of the microrods released from the microreactors in magnetic field even from dilute solution, and (iii) the protein coating on the internal walls of the microreactors is shown to generate molecular imprints on the surface of the polymeric rods. This latter property was demonstrated by comparative binding experiments of a fluorescent avidin derivative to the surface-imprinted and non-imprinted magnetic polymer microrods. LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Development of a malaria diagnostic platform using magnetically induced linear dichroism PY - 2012 UR - https://m2.mtmt.hu/api/publication/2913701 ID - 2913701 LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Determination of the secondary structure of a newly developed protein from circular dichroism spectroscopy PY - 2012 UR - https://m2.mtmt.hu/api/publication/2913682 ID - 2913682 LA - English DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - A malária diagnózisa mágnesesen indukált lineáris dikroizmus révén PY - 2012 UR - https://m2.mtmt.hu/api/publication/2913677 ID - 2913677 LA - Hungarian DB - MTMT ER - TY - GEN AU - Orbánová, Agnesa ED - Orbánová, Agnesa TI - Secondary structure of proteins from circular dichroism spectroscopy PY - 2010 UR - https://m2.mtmt.hu/api/publication/2913685 ID - 2913685 LA - English DB - MTMT ER -