TY  - JOUR
AU  - Pecze, L
AU  - Jósvay, Katalin
AU  - Blum, W
AU  - Petrovics, G
AU  - Vizler, Csaba
AU  - Oláh, Zoltán
AU  - Schwaller, B
TI  - Activation of endogenous TRPV1 fails to induce overstimulation-based cytotoxicity in breast and prostate cancer cells but not in pain-sensing neurons
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
J2  - BBA-MOL CELL RES
VL  - 1863
PY  - 2016
IS  - 8
SP  - 2054
EP  - 2064
PG  - 11
SN  - 0167-4889
DO  - 10.1016/j.bbamcr.2016.05.007
UR  - https://m2.mtmt.hu/api/publication/3095574
ID  - 3095574
N1  - Megjegyzés-27115000
N1 Funding details: 130680, SNF, Stavros Niarchos Foundation
AB  - Vanilloids including capsaicin and resiniferatoxin are potent transient receptor potential vanilloid type I (TRPV1) agonists. TRPV1 overstimulation selectively ablates capsaicin-sensitive sensory neurons in animal models in vivo. The cytotoxic mechanisms are based on strong Na+ and Ca2+ influx via TRPV1 channels, which leads to mitochondrial Ca2+ accumulation and necrotic cell swelling. Increased TRPV1 expression levels are also observed in breast and prostate cancer and derived cell lines. Here, we examined whether potent agonist-induced over stimulation mediated by TRPV1 might represent a means for the eradication of prostate carcinoma (PC-3, Du 145, LNCaP) and breast cancer (MCF7, MDA-MB-231, BT-474) cells in vitro. While rat sensory neurons were highly vanilloid-sensitive, normal rat prostate epithelial cells were resistant in vivo. We found TRPV1 to be expressed in all cancer cell lines at mRNA and protein levels, yet protein expression levels were significantly lower compared to sensory neurons. Treatment of all human carcinoma cell lines with capsaicin didn't lead to overstimulation cytotoxicity in vitro. We assume that the low vanilloid-sensitivity of prostate and breast cancer cells is associated with low expression levels of TRPV1, since ectopic TRPV1 expression rendered them susceptible to the cytotoxic effect of vanilloids evidenced by plateau-type Ca2+ signals, mitochondrial Ca2+ accumulation and Na+- and Ca2+-dependent membrane disorganization. Moreover, long-term monitoring revealed that merely the ectopic expression of TRPV1 stopped cell proliferation and often induced apoptotic processes via strong activation of caspase-3 activity. Our results indicate that specific targeting of TRPV1 function remains a putative strategy for cancer treatment. (C) 2016 The Authors. Published by Elsevier B.V.
LA  - English
DB  - MTMT
ER  -