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K1A 0R6, Canada; email: Lu.Yang@nrc.ca \n Chemicals/CAS: 1,1,1 trichloroethane, 71-55-6; carbon 13, 14762-74-4; chloroform, 67-66-3; mesylic acid, 2386-57-4, 75-75-2; methionine, 59-51-8, 63-68-3, 7005-18-7; selenomethionine, 1464-42-2, 3211-76-5; Carbon Isotopes; Mesylates; Methionine, 63-68-3; Selenomethionine, 1464-42-2; methanesulfonic acid, 75-75-2", "unhandledTickets" : 0, "deleted" : false, "lastRefresh" : "2024-02-19T20:25:17.145+0000", "lastModified" : "2020-12-23T08:16:13.302+0000", "created" : "2019-01-15T17:53:31.781+0000", "creator" : { "otype" : "Author", "mtid" : 10066231, "link" : "/api/author/10066231", "label" : "Mester Zoltán (Kémia)", "familyName" : "Mester", "givenName" : "Zoltán", "published" : true, "snippet" : true }, "duplumSearchResult" : { "id" : 4452896, "creationDate" : "2023-02-21T20:34:24.961+0000", "startDate" : "2023-02-21T20:34:25.100+0000", "creator" : { "otype" : "Author", "mtid" : 10066231, "link" : "/api/author/10066231", "label" : "Mester Zoltán (Kémia)", "familyName" : "Mester", "givenName" : "Zoltán", "published" : true, "snippet" : true } }, "duplumRole" : "SUSPECT", "lastDuplumOK" : "2022-08-22T11:51:37.372+0000", "lastDuplumSearch" : "2023-02-21T20:34:25.100+0000", "core" : true, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Szakcikk (Folyóiratcikk)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Tudományos", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "Angol", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Yang, L.", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 85008908, "link" : "/api/authorship/85008908", "label" : "Yang, L.", "listPosition" : 1, "share" : 0.33333334, "first" : true, "last" : false, "familyName" : "Yang", "givenName" : "L.", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 85008909, "link" : "/api/authorship/85008909", "label" : "Mester, Z. 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"pageLength" : 8, "publishedYear" : 2004, "abstractText" : "A method for the simultaneous determination of methionine (Met) and selenomethionine (SeMet) in yeast using species-specific isotope dilution (ID) gas chromatography/mass spectrometry (GC/MS) is described. Samples were digested by refluxing for 16 h with 4 M methane-sulfonic acid. Analytes were derivatized with methyl chloroformate and extracted into chloroform for GC/MS analysis. In addition to use of commercially available 13C-enriched Met and SeMet spikes for species specific ID analysis, a 74Se-enriched SeMet spike was also available for comparison of results. In selective ion monitoring mode, the intensities of ions at m/z 221, 222, 269, 270, and 263 were used to calculate the 221/222, 269/270, and 269/263 ion ratios for quantification of Met and SeMet. Concentrations of 5959 ± 33 and 3404 ± 12 μg g -1 (one standard deviation, n = 6) with relative standard deviations of 0.55 and 0.36% for Met and SeMet, respectively, were obtained using 13C-enriched spikes. A concentration of 3417 ± 8 μg g -1 (one standard deviation, n = 6) was obtained using the 74Se-enriched SeMet spike. The concentration of SeMet measured in the yeast is equivalent to 66.43 ± 0.24% of total Se and 30.31 ± 0.11% of total Met is in the form of SeMet. Method detection limits (three times the standard deviation) of 3.4 and 1.0 μg g-1 were estimated for Met and SeMet, respectively, based on a 0.25-g subsample of yeast with 1 mL of extract used for derivatization. A similar concentration of 5930 ± 29 μg g-1 (one standard deviation, n = 4) for Met and a lower concentration of 2787 ± 49 μg g-1 (one standard deviation, n = 4) for SeMet were obtained for this yeast sample using species-specific ID analysis based on GC/MS with 13C-enriched Met and SeMet spikes when a 2-h open microwave digestion approach using 8 M methanesulfonic acid was used.", "keywords" : [ { "otype" : "Keyword", "mtid" : 112, "link" : "/api/keyword/112", "label" : "DERIVATIVES", "published" : true, "oldId" : 112, "snippet" : true }, { "otype" : "Keyword", "mtid" : 227, "link" : "/api/keyword/227", "label" : "EXTRACTION", "published" : true, "oldId" : 227, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1699, "link" : "/api/keyword/1699", "label" : "ARTICLE", "published" : true, "oldId" : 1699, "snippet" : true }, { "otype" : "Keyword", "mtid" : 2307, "link" : "/api/keyword/2307", "label" : "YEAST", "published" : true, "oldId" : 2307, "snippet" : true }, { "otype" : 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