@article{MTMT:2989146,
	title = {Probing pattern and dynamics of disulfide bridges using synthesis and NMR of an ion channel blocker peptide toxin with multiple diselenide bonds},
	url = {https://m2.mtmt.hu/api/publication/2989146},
	author = {Fehér, Krisztina and Timári, István and Rákosi, Kinga and Szolomájer, János and Tóthné Illyés, Tünde Zita and Bartók, Ádám and Varga, Zoltán and Panyi, György and Tóth, Gábor and E Kövér, Katalin},
	doi = {10.1039/C5SC03995A},
	journal-iso = {CHEM SCI},
	journal = {CHEMICAL SCIENCE},
	volume = {7},
	unique-id = {2989146},
	issn = {2041-6520},
	abstract = {Anuroctoxin (AnTx), a 35-amino-acid scorpion toxin contg. four disulfide bridges, is a high affinity blocker of the voltage-gated potassium channel Kv1.3, but also blocks Kv1.2. To improve the potential therapeutic use of the toxin, we have designed a double substituted analog, [N17A/F32T]-AnTx, which showed comparable Kv1.3 affinity to the wild-type peptide, but 2500-fold increase in the selectivity for Kv1.3 over Kv1.2. In the present study we have achieved the chem. synthesis of a Sec-analog in which all cysteine (Cys) residues have been replaced by selenocysteine (Sec) forming four diselenide bonds. To the best of our knowledge this is the first time to replace, by chem. synthesis, all disulfide bonds with isosteric diselenides in a peptide/protein. Gratifyingly, the key pharmacol. properties of the Sec-[N17A/F32T]-AnTx are retained since the peptide is functionally active. We also propose here a combined exptl. and theor. approach including NOE- and 77Se-based NMR supplemented by MD simulations for conformational and dynamic characterization of the Sec-[N17A/F32T]-AnTx. The use of such combined approach allowed us to attain unequivocal assignment of all four diselenide bonds and supplemental MD simulations allowed to characterize the conformational dynamics around each disulfide/diselenide bridge. [on SciFinder(R)]},
	year = {2016},
	eissn = {2041-6539},
	pages = {2666-2673},
	orcid-numbers = {Szolomájer, János/0000-0003-1458-6156; Bartók, Ádám/0000-0002-1232-5246; Panyi, György/0000-0001-6227-3301; Tóth, Gábor/0000-0002-3604-4385}
}