TY  - JOUR
AU  - E Kövér, Katalin
AU  - Wéber, Edit
AU  - Martinek, Tamás
AU  - Monostori, Éva
AU  - Batta, Gyula
TI  - 15N and 13C Group‐Selective Techniques Extend the Scope of STD NMR Detection of Weak Host–Guest Interactions and Ligand Screening
JF  - CHEMBIOCHEM
J2  - CHEMBIOCHEM
VL  - 11
PY  - 2010
IS  - 15
SP  - 2182
EP  - 2187
PG  - 6
SN  - 1439-4227
DO  - 10.1002/cbic.201000317
UR  - https://m2.mtmt.hu/api/publication/1452809
ID  - 1452809
N1  - Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary            
            Institute of Pharmaceutical Chemistry, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary            
            Lymphocyte Signal Transduction Laboratory, Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt. 62, 6726 Szeged, Hungary            
            Department of Organic Chemistry, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary            
            Cited By :9            
            Export Date: 8 September 2023            
            CODEN: CBCHF            
            Correspondence Address: Kövér, K.E.; Department of Inorganic and Analytical Chemistry, Egyetem tér 1, 4032 Debrecen, Hungary; email: kover@tigris.unideb.hu
AB  - Saturation transfer difference (STD) is a valuable tool for studying the binding of small molecules to large biomolecules and for obtaining detailed information on the binding epitopes. Here, we demonstrate that the proposed N-15/C-13 variants of group-selective, "GS-STD" experiments provide a powerful approach to mapping the binding epitope of a ligand even in the absence of efficient spin diffusion within the target protein. Therefore, these experimental variants broaden the scope of STD studies to smaller and/or more-dynamic targets. The STD spectra obtained in four different experimental setups (selective H-1 STD, N-15 GS-STD, C-13(Ar) and C-13(aliphatic) GS-STD approaches) revealed that the signal-intensity pattern of the difference spectra is affected by both the type and the spatial distribution of the excited "transmitter" atoms, as well as by the efficiency of the spin-diffusion-mediated magnetization transfer. The performance of the experiments is demonstrated on a system by using the lectin, galectin-1 and its carbohydrate ligand, lactose.
LA  - English
DB  - MTMT
ER  -