@article{MTMT:3335848,
	title = {Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes},
	url = {https://m2.mtmt.hu/api/publication/3335848},
	author = {Rónavári, Andrea and Igaz, Nóra and Gopisetty, Mohana Krishna and Szerencsés, Bettina and Kovács, Dávid and Papp, Csaba Gergő and Vágvölgyi, Csaba and Boros, Imre Miklós and Kónya, Zoltán and Csontné Kiricsi, Mónika and Pfeiffer, Ilona},
	doi = {10.2147/IJN.S152010},
	journal-iso = {INT J NANOMED},
	journal = {INTERNATIONAL JOURNAL OF NANOMEDICINE},
	volume = {13},
	unique-id = {3335848},
	issn = {1176-9114},
	abstract = {Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns and providing much of the impetus for current attempts to develop novel and efficient agents against cutaneous mycosis causing species. Innovative, environmentally benign and economic nanotechnology-based approaches have recently emerged utilizing principally biological sources to produce nano-sized structures with unique antimicrobial properties. In line with this, our aim was to generate silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) by biological synthesis and to study the effect of the obtained nanoparticles on cutaneous mycosis causing fungi and on human keratinocytes. Methods: Cell-free extract of the red yeast Phaffia rhodozyma proved to be suitable for nanoparticle preparation and the generated AgNPs and AuNPs were characterized by transmission electron microscopy, dynamic light scattering and X-ray powder diffraction. Results: Antifungal studies demonstrated that the biosynthesized silver particles were able to inhibit the growth of several opportunistic Candida or Cryptococcus species and were highly potent against filamentous Microsporum and Trichophyton dermatophytes. Among the tested species only Cryptococcus neoformans was susceptible to both AgNPs and AuNPs. Neither AgNPs nor AuNPs exerted toxicity on human keratinocytes. Conclusion: Our results emphasize the therapeutic potential of such biosynthesized nanoparticles, since their biocompatibility to skin cells and their outstanding antifungal performance can be exploited for topical treatment and prophylaxis of superficial cutaneous mycosis. © 2018 Rónavári et al.},
	keywords = {TOXICITY; Antifungal activity; Silver nanoparticles; Dermatophytes; BIOLOGICAL SYNTHESIS; Opportunistic pathogenic yeasts},
	year = {2018},
	eissn = {1178-2013},
	pages = {695-703},
	orcid-numbers = {Rónavári, Andrea/0000-0001-7054-0975; Igaz, Nóra/0000-0003-1580-4397; Gopisetty, Mohana Krishna/0000-0002-4310-3478; Papp, Csaba Gergő/0000-0003-4450-0667; Vágvölgyi, Csaba/0000-0003-0009-7773; Boros, Imre Miklós/0000-0001-8504-9687; Kónya, Zoltán/0000-0002-9406-8596; Csontné Kiricsi, Mónika/0000-0002-8416-2052; Pfeiffer, Ilona/0000-0003-0680-7596}
}

@article{MTMT:2367028,
	title = {Characterization of Virulence Properties in the C. parapsilosis Sensu Lato Species.},
	url = {https://m2.mtmt.hu/api/publication/2367028},
	author = {Németh, Tibor Mihály and Tóth, Adél and Szenzenstein, Judit and Horváth, Péter Ferenc and Nosanchuk, JD and Grózer, Zsuzsanna Barbara and Tóth, Renáta and Papp, Csaba Gergő and Kozma-Bognárné Hamari, Zsuzsanna and Vágvölgyi, Csaba and Gácser, Attila},
	doi = {10.1371/journal.pone.0068704},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {8},
	unique-id = {2367028},
	issn = {1932-6203},
	abstract = {The C. parapsilosis sensu lato group involves three closely related species, C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis. Although their overall clinical importance is dramatically increasing, there are few studies regarding the virulence properties of the species of the psilosis complex. In this study, we tested 63 C. parapsilosis sensu stricto, 12 C. metapsilosis and 18 C. orthopsilosis isolates for the ability to produce extracellular proteases, secrete lipases and form pseudohyphae. Significant differences were noted between species, with the C. metapsilosis strains failing to secrete lipase or to produce pseudohyphae. Nine different clinical isolates each of C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis were co-cultured with immortalized murine or primary human macrophages. C. parapsilosis sensu stricto isolates showed a significantly higher resistance to killing by primary human macrophages compared to C. orthopsilosis and C. metapsilosis isolates. In contrast, the killing of isolates by J774.2 mouse macrophages did not differ significantly between species. However, C. parapsilosis sensu stricto isolates induced the most damage to murine and human macrophages, and C. metapsilosis strains were the least toxic. Furthermore, strains that produced lipase or pseudohyphae were most resistant to macrophage-mediated killing and produced the most cellular damage. Finally, we used 9 isolates of each of the C. parapsilosis sensus lato species to examine their impact on the survival of Galleriamellonella larvae. The mortality rate of G. mellonella larvae infected with C. metapsilosis isolates was significantly lower than those infected with C. parapsilosis sensu stricto or C. orthopsilosis strains. Taken together, our findings demonstrate that C. metapsilosis is indeed the least virulent member of the psilosis group, and also highlight the importance of pseudohyphae and secreted lipases during fungal-host interactions.},
	year = {2013},
	eissn = {1932-6203},
	orcid-numbers = {Tóth, Renáta/0000-0001-7395-0689; Papp, Csaba Gergő/0000-0003-4450-0667; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083; Vágvölgyi, Csaba/0000-0003-0009-7773}
}

@article{MTMT:30376956,
	title = {Echinocandin-Induced Microevolution of Candida parapsilosis Influences Virulence and Abiotic Stress Tolerance},
	url = {https://m2.mtmt.hu/api/publication/30376956},
	author = {Papp, Csaba Gergő and Kocsis, Katica and Tóth, Renáta and Bodai, László and Willis, Jesse R. and Ksiezopolska, Ewa and Lozoya-Pérez, Nancy E. and Vágvölgyi, Csaba and Mora Montes, Hector and Gabaldón, Toni and Nosanchuk, Joshua D. and Gácser, Attila},
	doi = {10.1128/mSphere.00547-18},
	journal-iso = {MSPHERE},
	journal = {mSPHERE},
	volume = {3},
	unique-id = {30376956},
	issn = {2379-5042},
	year = {2018},
	eissn = {2379-5042},
	orcid-numbers = {Papp, Csaba Gergő/0000-0003-4450-0667; Tóth, Renáta/0000-0001-7395-0689; Bodai, László/0000-0001-8411-626X; Vágvölgyi, Csaba/0000-0003-0009-7773}
}

@article{MTMT:30332415,
	title = {In vivo applicability of Neosartorya fischeri antifungal protein 2 (NFAP2) in treatment of vulvovaginal candidiasis},
	url = {https://m2.mtmt.hu/api/publication/30332415},
	author = {Kovács, Renátó and Holzknecht, J and Hargitai, Z and Papp, Csaba Gergő and Farkas, Attila and Borics, Attila and Tóth, Liliána and Váradi, Györgyi and Tóth, Gábor and Kovács, Ilona and Dubrac, S and Majoros, László and Marx, F and Galgóczi, László Norbert},
	doi = {10.1128/AAC.01777-18},
	journal-iso = {ANTIMICROB AGENTS CH},
	journal = {ANTIMICROBIAL AGENTS AND CHEMOTHERAPY},
	volume = {63},
	unique-id = {30332415},
	issn = {0066-4804},
	abstract = {As a consequence of emerging numbers of vulvovaginitis cases caused by azole-resistant and biofilm-forming Candida species, fast and efficient treatment of this infection has become challenging. The problem is further exacerbated by the severe side effects of azoles as long-term-use medications in the recurrent form. There is therefore an increasing demand for novel and safely applicable effective antifungal therapeutic strategies. The small, cysteine-rich, and cationic antifungal proteins from filamentous ascomycetes are potential candidates, as they inhibit the growth of several Candida spp. in vitro; however, no information is available about their in vivo antifungal potency against yeasts. In the present study, we investigated the possible therapeutic application of one of their representatives in the treatment of vulvovaginal candidiasis, Neosartorya fischeri antifungal protein 2 (NFAP2). NFAP2 inhibited the growth of a fluconazole (FLC)-resistant Candida albicans strain isolated from a vulvovaginal infection, and it was effective against both planktonic cells and biofilm in vitro. We observed that the fungal cell-killing activity of NFAP2 is connected to its pore-forming ability in the cell membrane. NFAP2 did not exert cytotoxic effects on primary human keratinocytes and dermal fibroblasts at the MIC in vitro. In vivo murine vulvovaginitis model experiments showed that NFAP2 significantly decreases the number of FLC-resistant C. albicans cells, and combined application with FLC enhances the efficacy. These results suggest that NFAP2 provides a feasible base for the development of a fundamental new, safely applicable mono- or polytherapeutic topical agent for the treatment of superficial candidiasis.},
	year = {2019},
	eissn = {1098-6596},
	orcid-numbers = {Papp, Csaba Gergő/0000-0003-4450-0667; Tóth, Liliána/0000-0003-1400-6174; Váradi, Györgyi/0000-0001-7907-8908; Tóth, Gábor/0000-0002-3604-4385; Galgóczi, László Norbert/0000-0002-6976-8910}
}

@article{MTMT:31353819,
	title = {Size-dependent activity of silver nanoparticles on the morphological switch and biofilm formation of opportunistic pathogenic yeasts},
	url = {https://m2.mtmt.hu/api/publication/31353819},
	author = {Szerencsés, Bettina and Igaz, Nóra and Tóbiás, Ákos and Prucsi, Zsombor and Rónavári, Andrea and Bélteky, Péter and Madarász, Dániel and Papp, Csaba Gergő and Makra, Ildikó and Vágvölgyi, Csaba and Kónya, Zoltán and Pfeiffer, Ilona and Csontné Kiricsi, Mónika},
	doi = {10.1186/s12866-020-01858-9},
	journal-iso = {BMC MICROBIOL},
	journal = {BMC MICROBIOLOGY},
	volume = {20},
	unique-id = {31353819},
	issn = {1471-2180},
	year = {2020},
	eissn = {1471-2180},
	orcid-numbers = {Igaz, Nóra/0000-0003-1580-4397; Rónavári, Andrea/0000-0001-7054-0975; Papp, Csaba Gergő/0000-0003-4450-0667; Vágvölgyi, Csaba/0000-0003-0009-7773; Kónya, Zoltán/0000-0002-9406-8596; Pfeiffer, Ilona/0000-0003-0680-7596; Csontné Kiricsi, Mónika/0000-0002-8416-2052}
}

@article{MTMT:2802304,
	title = {Kinetic studies of Candida parapsilosis phagocytosis by macrophages and detection of intracellular survival mechanisms.},
	url = {https://m2.mtmt.hu/api/publication/2802304},
	author = {Tóth, Renáta and Tóth, Adél and Papp, Csaba Gergő and Jankovics, Ferenc and Vágvölgyi, Csaba and Alonso, MF and Bain, JM and Erwig, LP and Gácser, Attila},
	doi = {10.3389/fmicb.2014.00633},
	journal-iso = {FRONT MICROBIOL},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {5},
	unique-id = {2802304},
	issn = {1664-302X},
	abstract = {Even though the number of Candida infections due to non-albicans species like C. parapsilosis has been increasing, little is known about their pathomechanisms. Certain aspects of C. parapsilosis and host interactions have already been investigated; however we lack information about the innate cellular responses toward this species. The aim of our project was to dissect and compare the phagocytosis of C. parapsilosis to C. albicans and to another Candida species C. glabrata by murine and human macrophages by live cell video microscopy. We broke down the phagocytic process into three stages: macrophage migration, engulfment of fungal cells and host cell killing after the uptake. Our results showed increased macrophage migration toward C. parapsilosis and we observed differences during the engulfment processes when comparing the three species. The engulfment time of C. parapsilosis was comparable to that of C. albicans regardless of the pseudohypha length and spatial orientation relative to phagocytes, while the rate of host cell killing and the overall uptake regarding C. parapsilosis showed similarities mainly with C. glabrata. Furthermore, we observed difference between human and murine phagocytes in the uptake of C. parapsilosis. UV-treatment of fungal cells had varied effects on phagocytosis dependent upon which Candida strain was used. Besides statistical analysis, live cell imaging videos showed that this species similarly to the other two also has the ability to survive in host cells via the following mechanisms: yeast replication, and pseudohypha growth inside of phagocytes, exocytosis of fungal cells and also abortion of host cell mitosis following the uptake. According to our knowledge this is the first study that provides a thorough examination of C. parapsilosis phagocytosis and reports intracellular survival mechanisms associated with this species.},
	year = {2014},
	eissn = {1664-302X},
	orcid-numbers = {Tóth, Renáta/0000-0001-7395-0689; Papp, Csaba Gergő/0000-0003-4450-0667; Vágvölgyi, Csaba/0000-0003-0009-7773}
}