{ "labelLang" : "eng", "responseDate" : "2024-03-28 09:19", "paging" : { "last" : true, "first" : true, "totalPages" : 1, "totalElements" : 15, "totalUncutElements" : 15, "totalEstimatedElements" : 20, "size" : 100, "number" : 1, "numberOfElements" : 15, "sort" : [ { "direction" : "DESC", "property" : "publishedYear", "ascending" : false }, { "direction" : "ASC", "property" : "firstAuthor", "ascending" : true } ] }, "content" : [ { "otype" : "JournalArticle", "mtid" : 34232792, "status" : "VALIDATED", "published" : true, "unhandledTickets" : 0, "deleted" : false, "lastRefresh" : "2024-02-23T12:07:20.214+0000", "lastModified" : "2024-01-29T08:05:29.694+0000", "created" : "2023-11-02T14:03:25.211+0000", "creator" : { "otype" : "Author", "mtid" : 10015499, "link" : "/api/author/10015499", "label" : "László Csanády (Biokémia)", "familyName" : "Csanády", "givenName" : "László", "published" : true, "oldId" : 10015499, "snippet" : true }, "lastDuplumSearch" : "2024-02-17T07:04:09.323+0000", "validated" : "2024-01-29T08:05:30.017+0000", "validator" : { "otype" : "Admin", "mtid" : 10080205, "link" : "/api/admin/10080205", "label" : "Ágnes Szalóky-Siki (SE_KK_Admin5_SZSA, admin)", "familyName" : "Szalóky-Siki", "givenName" : "Ágnes", "published" : true, "snippet" : true }, "core" : true, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Journal Article", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Article (Journal Article)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Journal Article", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Scientific", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "English", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Simon, Márton A", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 111064555, "link" : "/api/authorship/111064555", "label" : "Simon, Márton A [Simon, Márton (Biokémia), author] Biokémiai és Molekuláris Biológiai Intézet (SU / FM / I); Biokémiai Tanszék (SU / FM / I / BMBI); HCEMM-SU Molecular Channelopathies Research Group (SU / FM / I / BMBI / BT)", "listPosition" : 1, "share" : 0.25, "first" : true, "last" : false, "corresponding" : false, "author" : { "otype" : "Author", "mtid" : 10066737, "link" : "/api/author/10066737", "label" : "Márton Simon (Biokémia)", "familyName" : "Simon", "givenName" : "Márton", "published" : true, "snippet" : true }, "familyName" : "Simon", "givenName" : "Márton A", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Author", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 111064556, "link" : "/api/authorship/111064556", "label" : "Iordanov, Iordan [Iordanov, Iordan (biokémia), author] Biokémiai és Molekuláris Biológiai Intézet (SU / FM / I); Biokémiai Tanszék (SU / FM / I / BMBI); HUN-REN-SE Ion Channel Research Group (SU / FM / I / BMBI / BT); HCEMM-SU Molecular Channelopathies Research Group (SU / FM / I / BMBI / BT)", "listPosition" : 2, "share" : 0.25, "first" : false, "last" : false, "corresponding" : false, "author" : { "otype" : "Author", "mtid" : 10052053, "link" : "/api/author/10052053", "label" : "Iordan Iordanov (biokémia)", "familyName" : "Iordanov", "givenName" : "Iordan", "published" : true, "oldId" : 10052053, "snippet" : true }, "familyName" : "Iordanov", "givenName" : "Iordan", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Author", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 111064557, "link" : "/api/authorship/111064557", "label" : "Szollosi, Andras [Szöllősi, András (Biokémia), author] Biokémiai és Molekuláris Biológiai Intézet (SU / FM / I); Biokémiai Tanszék (SU / FM / I / BMBI); HUN-REN-SE Ion Channel Research Group (SU / FM / I / BMBI / BT); HCEMM-SU Molecular Channelopathies Research Group (SU / FM / I / BMBI / BT)", "listPosition" : 3, "share" : 0.25, "first" : false, "last" : false, "corresponding" : false, "author" : { "otype" : "Author", "mtid" : 10020191, "link" : "/api/author/10020191", "label" : "András Szöllősi (Biokémia)", "familyName" : "Szöllősi", "givenName" : "András", "published" : true, "oldId" : 10020191, "snippet" : true }, "familyName" : "Szollosi", "givenName" : "Andras", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Author", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 111064558, "link" : "/api/authorship/111064558", "label" : "Csanády, László ✉ [Csanády, László (Biokémia), author] Biokémiai és Molekuláris Biológiai Intézet (SU / FM / I); Biokémiai Tanszék (SU / FM / I / BMBI); HUN-REN-SE Ion Channel Research Group (SU / FM / I / BMBI / BT); HCEMM-SU Molecular Channelopathies Research Group (SU / FM / I / BMBI / BT)", "listPosition" : 4, "share" : 0.25, "first" : false, "last" : true, "corresponding" : true, "author" : { "otype" : "Author", "mtid" : 10015499, "link" : "/api/author/10015499", "label" : "László Csanády (Biokémia)", "familyName" : "Csanády", "givenName" : "László", "published" : true, "oldId" : 10015499, "snippet" : true }, "familyName" : "Csanády", "givenName" : "László", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Author", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true } ], "title" : "Estimating the true stability of the prehydrolytic outward-facing state in an ABC protein.", "identifiers" : [ { "otype" : "PublicationIdentifier", "mtid" : 24612551, "link" : "/api/publicationidentifier/24612551", "label" : "DOI: 10.7554/eLife.90736", "source" : { "otype" : "PlainSource", "mtid" : 6, "link" : "/api/publicationsource/6", "label" : "DOI", "type" : { "otype" : "PublicationSourceType", "mtid" : 10001, "link" : "/api/publicationsourcetype/10001", "label" : "DOI", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "DOI", "nameEng" : "DOI", "linkPattern" : "https://doi.org/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 6, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "10.7554/eLife.90736", "realUrl" : "https://doi.org/10.7554/eLife.90736", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 25378278, "link" : "/api/publicationidentifier/25378278", "label" : "WoS: 001134823500001", "source" : { "otype" : "PlainSource", "mtid" : 1, "link" : "/api/publicationsource/1", "label" : "WoS", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "WoS", "nameEng" : "WoS", "linkPattern" : "https://www.webofscience.com/wos/woscc/full-record/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 1, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "001134823500001", "realUrl" : "https://www.webofscience.com/wos/woscc/full-record/001134823500001", "published" : true, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 24612552, "link" : "/api/publicationidentifier/24612552", "label" : "Scopus: 85174752039", "source" : { "otype" : "PlainSource", "mtid" : 3, "link" : "/api/publicationsource/3", "label" : "Scopus", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "Scopus", "linkPattern" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@", "publiclyVisible" : true, "published" : true, "oldId" : 3, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "85174752039", "realUrl" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85174752039", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 24612550, "link" : "/api/publicationidentifier/24612550", "label" : "PubMed: 37782012", "source" : { "otype" : "PlainSource", "mtid" : 17, "link" : "/api/publicationsource/17", "label" : "PubMed", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "PubMed", "nameEng" : "PubMed", "linkPattern" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract", "publiclyVisible" : true, "published" : true, "oldId" : 17, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "37782012", "realUrl" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=37782012&dopt=Abstract", "published" : false, "snippet" : true } ], "journal" : { "otype" : "Journal", "mtid" : 10031024, "link" : "/api/journal/10031024", "label" : "ELIFE 2050-084X 2050-084X", "pIssn" : "2050-084X", "eIssn" : "2050-084X", "reviewType" : "REVIEWED", "noIF" : false, "sciIndexed" : true, "scopusIndexed" : true, "lang" : "FOREIGN", "hungarian" : false, "published" : true, "oldId" : 10031024, "snippet" : true }, "volume" : "12", "internalId" : "e90736", "firstPageOrInternalIdForSort" : "e90736", "pageLength" : 19, "publishedYear" : 2023, "abstractText" : "CFTR, the anion channel mutated in cystic fibrosis patients, is a model ABC protein whose ATP-driven conformational cycle is observable at single-molecule level in patch-clamp recordings. Bursts of CFTR pore openings are coupled to tight dimerization of its two nucleotide-binding domains (NBDs) and in wild-type (WT) channels are mostly terminated by ATP hydrolysis. The slow rate of non-hydrolytic closure - which determines how tightly bursts and ATP hydrolysis are coupled - is unknown, as burst durations of catalytic site mutants span a range of ~200-fold. Here, we show that Walker A mutation K1250A, Walker B mutation D1370N, and catalytic glutamate mutations E1371S and E1371Q all completely disrupt ATP hydrolysis. True non-hydrolytic closing rate of WT CFTR approximates that of K1250A and E1371S. That rate is slowed ~15-fold in E1371Q by a non-native inter-NBD H-bond, and accelerated ~15-fold in D1370N. These findings uncover unique features of the NBD interface in human CFTR.", "fundings" : [ { "otype" : "Funding", "mtid" : 2036410, "link" : "/api/funding/2036410", "label" : "(KKP 144199)", "published" : false, "snippet" : true }, { "otype" : "Funding", "mtid" : 2036411, "link" : "/api/funding/2036411", "label" : "(UNKP-22-3-II-SE-12)", "published" : false, "snippet" : true }, { "otype" : "Funding", "mtid" : 2036412, "link" : "/api/funding/2036412", "label" : "(CSANAD21G0)", "published" : false, "snippet" : true } ], "keywords" : [ { "otype" : "Keyword", "mtid" : 1519, "link" : "/api/keyword/1519", "label" : "ZEBRAFISH", "published" : true, "oldId" : 1519, "snippet" : true }, { "otype" : "Keyword", "mtid" : 7673, "link" : "/api/keyword/7673", "label" : "Xenopus", "published" : true, "oldId" : 7673, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1066395, "link" : "/api/keyword/1066395", "label" : "molecular biophysics", "published" : true, "oldId" : 1066395, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1175577, "link" : "/api/keyword/1175577", "label" : "Structural biology", "published" : true, "oldId" : 1175577, "snippet" : true }, { "otype" : "Keyword", "mtid" : 1264463, "link" : "/api/keyword/1264463", "label" : "D-loop", "published" : true, "oldId" : 1264463, "snippet" : true }, { "otype" : "Keyword", "mtid" : 3168070, "link" : "/api/keyword/3168070", "label" : "composite ATP-binding site", "published" : true, "snippet" : true }, { "otype" : "Keyword", "mtid" : 3168071, "link" : "/api/keyword/3168071", "label" : "flickery closure", "published" : true, "snippet" : true }, { "otype" : "Keyword", "mtid" : 3168072, "link" : "/api/keyword/3168072", "label" : "mutant cycle", "published" : true, "snippet" : true } ], "digital" : true, "printed" : null, "sourceYear" : 2023, "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : true, "missingAuthor" : false, "oaType" : "GOLD", "oaCheckDate" : "2024-02-23", "oaFree" : false, "oaLink" : "http://elife.elifesciences.org/", "citationCount" : 0, "citationCountUnpublished" : 0, "citationCountWoOther" : 0, "independentCitCountWoOther" : 0, "nationalOriginCitationCount" : 0, "foreignEditionCitationCount" : 0, "doiCitationCount" : 0, "wosCitationCount" : 0, "scopusCitationCount" : 0, "wosScopusCitationCount" : 0, "wosScopusCitationCountWoOther" : 0, "wosScopusIndependentCitationCount" : 0, "wosScopusIndependentCitationCountWoOther" : 0, "independentCitationCount" : 0, "selfCitationCount" : 0, "unhandledCitationCount" : 0, "citingPubCount" : 0, "independentCitingPubCount" : 0, "citingPubCountWoOther" : 0, "independentCitingPubCountWoOther" : 0, "unhandledCitingPubCount" : 0, "citedPubCount" : 12, "citedCount" : 12, "ratings" : [ { "otype" : "SjrRating", "mtid" : 11358162, "link" : "/api/sjrrating/11358162", "label" : "sjr:D1 (2023) Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous) ELIFE 2050-084X 2050-084X", "listPos" : 14, "rankValue" : 0.1, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 1301, "link" : "/api/classificationexternal/1301", "label" : "Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)", "published" : true, "oldId" : 1301, "snippet" : true }, "ranking" : "D1", "calculation" : "FROM_LAST_YEAR", "published" : true, "snippet" : true } ], "ratingsForSort" : "D1", "hasCitationDuplums" : false, "inSelectedPubs" : "10015499,10020191", "userChangeableUntil" : "2023-11-06T12:07:55.231+0000", "directInstitutesForSort" : "Biokémiai Tanszék (SE / AOK / I / BMBI); Biokémiai és Molekuláris Biológiai Intézet (SE / AOK / I); HCEMM-SE Molekuláris Csatornabetegségek Kutatóc... (SE / AOK / I / BMBI / BT); HUN-REN-SE Ioncsatorna Kutatócsoport (SE / AOK / I / BMBI / BT)", "ownerAuthorCount" : 4, "ownerInstituteCount" : 18, "directInstituteCount" : 4, "authorCount" : 4, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/34232792", "label" : "Simon Márton A et al. Estimating the true stability of the prehydrolytic outward-facing state in an ABC protein.. (2023) ELIFE 2050-084X 2050-084X 12", "template" : "