TY - JOUR AU - Bende, Gábor AU - Zsindely, Nóra AU - Laczi, Krisztián AU - Kristóffy, Zsolt AU - Papp, Csaba Gergő AU - Farkas, Attila AU - Tóth, Liliána AU - Sáringer, Szabolcs AU - Bodai, László AU - Rákhely, Gábor AU - Marx, Florentine AU - Galgóczi, László Norbert TI - The Neosartorya (Aspergillus) fischeri antifungal protein NFAP2 has low potential to trigger resistance development in Candida albicans in vitro JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 13 PY - 2025 IS - 1 PG - 22 SN - 2165-0497 DO - 10.1128/spectrum.01273-24 UR - https://m2.mtmt.hu/api/publication/35590762 ID - 35590762 N1 - Funding Agency and Grant Number: Nemzeti Kutatsi Fejlesztsi s Innovcis Hivatal (NKFI) [FK 134343, K 146131]; Hungarian National Research, Development and Innovation Office-NKFIH [I3132-B21, 7051, CA21145]; Austrian Science Fund FWF; COST (European Cooperation in Science and Technology) Funding text: The present work of L.G. was financed by the Hungarian National Research, Development and Innovation Office-NKFIH, FK 134343, and K 146131 projects. The research was funded in part by the Austrian Science Fund FWF (I3132-B21) to F.M. University of Szeged Open Access Fund, Grant ID: 7051. This article/publication is based upon work from COST Action EURESTOP, CA21145, supported by COST (European Cooperation in Science and Technology). AB - Due to the increase in the number of drug-resistant Candida albicans strains, new antifungal compounds with limited potential for the development of resistance are urgently needed. NFAP2, an antifungal protein (AFP) secreted by Neosartorya (Aspergillus) fischeri, is a promising candidate. We investigated the ability of C. albicans to develop resistance to NFAP2 in a microevolution experiment compared with generic fluconazole (FLC). C. albicans adapted to only 1× minimum inhibitory concentration (MIC) of NFAP2, which can be considered tolerance rather than resistance, compared with 32× MIC of FLC. Genome analysis revealed non-silent mutations in only two genes in NFAP2-tolerant strains and in several genes in FLC-resistant strains. Tolerance development to NFAP2 did not influence cell morphology. The susceptibility of NFAP2-tolerant strains did not change to FLC, amphotericin B, micafungin, and terbinafine. These strains did not show altered susceptibility to AFPs from Penicillium chrysogenum, except one which had less susceptibility to Penicillium chrysogenum antifungal protein B. FLC-resistant strains had decreased susceptibility to terbinafine and NFAP2, but not to other drugs and AFPs from P. chrysogenum. NFAP2-tolerant and FLC-resistant strains showed decreased and increased NFAP2 binding and uptake, respectively. The development of tolerance to NFAP2 decreased tolerance to cell wall, heat, and UV stresses. The development of FLC resistance increased tolerance to cell wall stress and decreased tolerance to heat and UV stresses. Tolerance to NFAP2 did not have significant metabolic fitness cost and could not increase virulence, compared with resistance to FLC. LA - English DB - MTMT ER - TY - JOUR AU - Bodai, László AU - Borosta, Roberta AU - Ferencz, Ágnes AU - Kovács, Mercédesz AU - Zsindely, Nóra TI - The role of miR-137 in neurodegenerative disorders JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 13 PG - 16 SN - 1661-6596 DO - 10.3390/ijms25137229 UR - https://m2.mtmt.hu/api/publication/35212546 ID - 35212546 AB - Neurodegenerative diseases affect an increasing part of the population of modern societies, burdening healthcare systems and causing immense suffering at the personal level. The pathogenesis of several of these disorders involves dysregulation of gene expression, which depends on several molecular processes ranging from transcription to protein stability. microRNAs (miRNAs) are short non-coding RNA molecules that modulate gene expression by suppressing the translation of partially complementary mRNAs. miR-137 is a conserved, neuronally enriched miRNA that is implicated in neurodegeneration. Here, we review the current body of knowledge about the role that miR-137 plays in five prominent neurodegenerative disorders, including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and multiple sclerosis. The presented data indicate that, rather than having a general neuroprotective role, miR-137 modulates the pathology of distinct disorders differently. LA - English DB - MTMT ER - TY - CONF AU - Kovács, Mercédesz AU - Farkas, Anita AU - Siági, Fruzsina AU - Zsindely, Nóra AU - Nagy, Gábor AU - Bodai, László TI - miRNS-ek kifejeződésének és hatásának vizsgálata neurodegeneratív betegség modellekben T2 - Genetikai Műhelyek Magyarországon XXIII. Konferencia Programfüzet PY - 2024 SP - 20 UR - https://m2.mtmt.hu/api/publication/35415401 ID - 35415401 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kukri, András AU - Czékus, Zalán AU - Gallé, Ágnes AU - Nagy, Gábor AU - Zsindely, Nóra AU - Bodai, László AU - Galgóczi, László Norbert AU - Hamow, Kamirán Áron AU - Szalai, Gabriella AU - Ördög, Attila AU - Poór, Péter TI - Exploring the effects of red light night break on the defence mechanisms of tomato against fungal pathogen Botrytis cinerea JF - PHYSIOLOGIA PLANTARUM J2 - PHYSIOL PLANTARUM VL - 176 PY - 2024 IS - 4 PG - 19 SN - 0031-9317 DO - 10.1111/ppl.14504 UR - https://m2.mtmt.hu/api/publication/35260445 ID - 35260445 AB - Plant infections caused by fungi lead to significant crop losses worldwide every year. This study aims to better understand the plant defence mechanisms regulated by red light, in particular, the effects of red light at night when most phytopathogens are highly infectious. Our results showed that superoxide production significantly increased immediately after red light exposure and, together with hydrogen peroxide levels, was highest at dawn after 30 min of nocturnal red‐light treatment. In parallel, red‐light‐induced expression and increased the activities of several antioxidant enzymes. The nocturnal red light did not affect salicylic acid but increased jasmonic acid levels immediately after illumination, whereas abscisic acid levels increased 3 h after nocturnal red‐light exposure at dawn. Based on the RNAseq data, red light immediately increased the transcription of several chloroplastic chlorophyll a‐b binding protein and circadian rhythm‐related genes, such as Constans 1 , CONSTANS interacting protein 1 and zinc finger protein CONSTANS‐LIKE 10. In addition, the levels of several transcription factors were also increased after red light exposure, such as the DOF zinc finger protein and a MYB transcription factor involved in the regulation of circadian rhythms and defence responses in tomato. In addition to identifying these key transcription factors in tomato, the application of red light at night for one week not only reactivated key antioxidant enzymes at the gene and enzyme activity level at dawn but also contributed to a more efficient and successful defence against Botrytis cinerea infection. LA - English DB - MTMT ER - TY - CONF AU - Kukri, András AU - Czékus, Zalán AU - Gallé, Ágnes AU - Nagy, Gábor AU - Zsindely, Nóra AU - Bodai, László AU - Galgóczy, László AU - Hamow, Kamirán Áron AU - Szalai, Gabriella AU - Ördög, Attila AU - Poór, Péter TI - The role of ethylene in the red light-induced defence responses of tomato against Botrytis cinerea infection T2 - XIV. Magyar Növénybiológiai Kongresszus : összefoglaló kötet PB - Szegedi Biológiai Kutatóközpont C1 - Szeged SN - 9786156833006 PY - 2024 SP - 82 EP - 82 PG - 1 UR - https://m2.mtmt.hu/api/publication/35227587 ID - 35227587 LA - English DB - MTMT ER - TY - CONF AU - Kukri, András AU - Czékus, Zalán AU - Gallé, Ágnes AU - Nagy, Gábor AU - Zsindely, Nóra AU - Bodai, László AU - Galgóczi, László Norbert AU - Hamow, Kamirán Áron AU - Szalai, Gabriella AU - Ördög, Attila AU - Poór, Péter TI - Éjszakai vörös fény kezelés hatásának vizsgálata paradicsom növények védekezésének fokozására T2 - XIV. Magyar Növénybiológiai Kongresszus : összefoglaló kötet PB - Szegedi Biológiai Kutatóközpont C1 - Szeged SN - 9786156833006 PY - 2024 SP - 19 EP - 19 PG - 1 UR - https://m2.mtmt.hu/api/publication/35219259 ID - 35219259 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Apjok, Gábor AU - Számel, Mónika AU - Christodoulou, Chryso AU - Seregi, Viktória AU - Vásárhelyi, Bálint Márk AU - Stirling, Tamás AU - Eszenyi, Bálint Dénes AU - Sári, Tóbiás AU - Vidovics, Fanni AU - Nagrand, Erika AU - Kovács, Dorina AU - Szili, Petra AU - Lantos, Ildikó Ilona AU - Méhi, Orsolya Katinka AU - Jangir, Pramod Kumar AU - Herczeg, Róbert AU - Gálik, Bence AU - Urbán, Péter AU - Gyenesei, Attila AU - Draskovits, Gábor AU - Nyerges, Ákos AU - Fekete, Gergely AU - Bodai, László AU - Zsindely, Nóra AU - Dénes, Béla AU - Yosef, Ido AU - Qimron, Udi AU - Papp, Balázs AU - Pál, Csaba AU - Kintses, Bálint TI - Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains JF - NATURE MICROBIOLOGY J2 - NAT MICROBIOL VL - 8 PY - 2023 IS - 3 SP - 410 EP - 423 PG - 14 SN - 2058-5276 DO - 10.1038/s41564-023-01320-2 UR - https://m2.mtmt.hu/api/publication/33634821 ID - 33634821 N1 - Funding Agency and Grant Number: National Laboratory of Biotechnology Grants [NKFIH-871-3/2020, 2022-2.1.1-NL-2022-00008]; European Union [754432]; European Research Council [648364, 862077]; National Research, Development and Innovation Office grant [FK-135245, FK-124254]; National Research, Development and Innovation Office; Ministry for Innovation and Technology [KKP 129814, 126506]; New National Excellence Program of the Ministry of Human Capacities [UNKP-20-5-SZTE-654, UNKP-21-5-SZTE-579]; New National Excellence Program of the Ministry for Innovation and Technology - National Research, Development and Innovation Fund [UNKP-20-3 -SZTE-452]; Doctoral Student Scholarship Program of the Co-Operative Doctoral Program of the Ministry of Innovation and Technology - National Research, Development and Innovation Fund [KDP-17-4/ PALY-2021, C992025]; European Union's Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant [754432]; Polish Ministry of Science and Higher Education; National Academy of Scientist Education Program of the National Biomedical Foundation under Hungarian Ministry of Culture and Innovation; National Laboratory for Health Security [RRF-2.3.1-212022-00006, GINOP-2.3.2-15-2016-00014, GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00035]; Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences [BO/352/20, BO/00303/19/8]; [GINOP-2.3.4-15-2020-00010]; [GINOP-2.3.1-20-2020-00001]; [BECOMING-2019-1-HU01-KA203-061251] Funding text: We thank D. Verma from the Department of Microbiology and B. Bhimrao of Ambedkar University, Lucknow, India for help with soil sample collection and NBA approval. This work was supported by National Laboratory of Biotechnology Grants NKFIH-871-3/2020 and 2022-2.1.1-NL-2022-00008 (B.K. and C.P.); the European Union's Horizon 2020 research and innovation programme under grant agreement no. 739593 (B.P. and B.K.); the European Research Council H2020-ERC-2014-CoG 648364-Resistance Evolution (C.P.) and H2020-ERC-2019-PoC 862077-Aware (C.P.); National Research, Development and Innovation Office grant FK-135245 (B.K.) and FK-124254 (O.M.); the National Research, Development and Innovation Office and the Ministry for Innovation and Technology under the `Frontline' Programme KKP 129814 and 126506 (B.P. and C.P.); the National Laboratory for Health Security RRF-2.3.1-212022-00006 (B.P.), GINOP-2.3.2-15-2016-00014 (EVOMER, C.P. and B.P.), GINOP-2.3.2-15-2016-00020 (MolMedEx TUMORDNS, C.P.), GINOP-2.3.2-15-2016-00035 (N.Z.); a Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences (BO/352/20 (B.K.), BO/00303/19/8 (O.M)); New National Excellence Program of the Ministry of Human Capacities (UNKP-20-5-SZTE-654 and UNKP-215-SZTE-579, B.K.); New National Excellence Program of the Ministry for Innovation and Technology funded by the National Research, Development and Innovation Fund (UNKP-20-3 -SZTE-452, G.A.); the Doctoral Student Scholarship Program of the Co-Operative Doctoral Program of the Ministry of Innovation and Technology financed by the National Research, Development and Innovation Fund (KDP-17-4/ PALY-2021, C992025, M.S.). R.H., B.G., P.U. and A.G. were supported by GINOP-2.3.4-15-2020-00010, GINOP-2.3.1-20-2020-00001, BECOMING-2019-1-HU01-KA203-061251, the European Union's Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 754432 and the Polish Ministry of Science and Higher Education, from the financial resources for science in 2018-2023. This research work was conducted with the support of the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation (D.K.). AB - Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but the range of suitable host bacterial species is limited. This limitation affects both the scope of the identified ARGs and the interpretation of their clinical relevance. Here we present a functional metagenomics pipeline called Reprogrammed Bacteriophage Particle Assisted Multi-species Functional Metagenomics (DEEPMINE). This approach combines and improves the use of T7 bacteriophage with exchanged tail fibres and targeted mutagenesis to expand phage host-specificity and efficiency for functional metagenomics. These modified phage particles were used to introduce large metagenomic plasmid libraries into clinically relevant bacterial pathogens. By screening for ARGs in soil and gut microbiomes and clinical genomes against 13 antibiotics, we demonstrate that this approach substantially expands the list of identified ARGs. Many ARGs have species-specific effects on resistance; they provide a high level of resistance in one bacterial species but yield very limited resistance in a related species. Finally, we identified mobile ARGs against antibiotics that are currently under clinical development or have recently been approved. Overall, DEEPMINE expands the functional metagenomics toolbox for studying microbial communities. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Henrietta AU - Varga, Viktória AU - Bodai, László AU - Zsindely, Nóra AU - Nagy, Gábor AU - Hegedűs, Zoltán AU - Hultmark, Dan AU - Andó, István TI - Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2023.1322381 UR - https://m2.mtmt.hu/api/publication/34446740 ID - 34446740 N1 - * Megosztott szerzőség AB - Background: Insects have specialized cell types that participate in the elimination of parasites, for instance, the lamellocytes of the broadly studied species Drosophila melanogaster. Other drosophilids, such as Drosophila ananassae and the invasive Zaprionus indianus, have multinucleated giant hemocytes, a syncytium of blood cells that participate in the encapsulation of the eggs or larvae of parasitoid wasps. These cells can be formed by the fusion of hemocytes in circulation or originate from the lymph gland. Their ultrastructure highly resembles that of the mammalian megakaryocytes. Methods: Morphological, protein expressional, and functional features of blood cells were revealed using epifluorescence and confocal microscopy. The respective hemocyte subpopulations were identified using monoclonal antibodies in indirect immunofluorescence assays. Fluorescein isothiocyanate (FITC)-labeled Escherichia coli bacteria were used in phagocytosis tests. Gene expression analysis was performed following mRNA sequencing of blood cells. Results: D. ananassae and Z. indianus encapsulate foreign particles with the involvement of multinucleated giant hemocytes and mount a highly efficient immune response against parasitoid wasps. Morphological, protein expressional, and functional assays of Z. indianus blood cells suggested that these cells could be derived from large plasmatocytes, a unique cell type developing specifically after parasitoid wasp infection. Transcriptomic analysis of blood cells, isolated from naïve and wasp-infected Z. indianus larvae, revealed several differentially expressed genes involved in signal transduction, cell movements, encapsulation of foreign targets, energy production, and melanization, suggesting their role in the anti-parasitoid response. A large number of genes that encode proteins associated with coagulation and wound healing, such as phenoloxidase activity factor-like proteins, fibrinogen-related proteins, lectins, and proteins involved in the differentiation and function of platelets, were constitutively expressed. The remarkable ultrastructural similarities between giant hemocytes and mammalian megakaryocytes, and presence of platelets, and giant cell- derived anucleated fragments at wound sites hint at the involvement of this cell subpopulation in wound healing processes, in addition to participation in the encapsulation reaction. Conclusion: Our observations provide insights into the broad repertoire of blood cell functions required for efficient defense reactions to maintain the homeostasis of the organism. The analysis of the differentiation and function of multinucleated giant hemocytes gives an insight into the diversification of the immune mechanisms. LA - English DB - MTMT ER - TY - CONF AU - Szebenyi, Csilla AU - Kocsubé, Sándor AU - Molnár, Anna AU - Kovács, Vanda AU - Csilla, Masa AU - Kiss, Karina AU - Yiyou, Gu AU - Ashraf, S Ibrahim AU - Sinka, Rita AU - Roland, Patai AU - Bodai, László AU - Nagy, Gábor AU - Kiss-Vetráb, Sándor AU - Kocsis, Dominik Sándor AU - Rafael, Bence AU - Abu Saleem, Tammam Khaliefeh Siliman AU - Vágvölgyi, Csaba AU - Papp, Tamás AU - Nagy, Gábor TI - CotH genes are necessary for normal spore formation and virulence in Mucor lusitanicusc T2 - 16th European Conference on Fungal Genetics: Programme & Abstracts PB - Universität Innsbruck C1 - Innsbruck PY - 2023 SP - 879 EP - 880 PG - 2 UR - https://m2.mtmt.hu/api/publication/33704383 ID - 33704383 LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Anita AU - Zsindely, Nóra AU - Nagy, Gábor AU - Kovács, Levente AU - Deák, Péter AU - Bodai, László TI - The ubiquitin thioesterase YOD1 ameliorates mutant Huntingtin induced pathology in Drosophila JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-49241-8 UR - https://m2.mtmt.hu/api/publication/34431874 ID - 34431874 AB - Huntington’s disease (HD) is a neurodegenerative disorder caused by a dominant gain-of-function mutation in the huntingtin gene, resulting in an elongated polyglutamine repeat in the mutant Huntingtin (mHtt) that mediates aberrant protein interactions. Previous studies implicated the ubiquitin–proteasome system in HD, suggesting that restoring cellular proteostasis might be a key element in suppressing pathology. We applied genetic interaction tests in a Drosophila model to ask whether modulating the levels of deubiquitinase enzymes affect HD pathology. By testing 32 deubiquitinase genes we found that overexpression of Yod1 ameliorated all analyzed phenotypes, including neurodegeneration, motor activity, viability, and longevity. Yod1 did not have a similar effect in amyloid beta overexpressing flies, suggesting that the observed effects might be specific to mHtt. Yod1 overexpression did not alter the number of mHtt aggregates but moderately increased the ratio of larger aggregates. Transcriptome analysis showed that Yod1 suppressed the transcriptional effects of mHtt and restored the expression of genes involved in neuronal plasticity, vesicular transport, antimicrobial defense, and protein synthesis, modifications, and clearance. Furthermore, Yod1 overexpression in HD flies leads to the upregulation of genes involved in transcriptional regulation and synaptic transmission, which might be part of a response mechanism to mHtt-induced stress. LA - English DB - MTMT ER -