@article{MTMT:34779108, title = {Diterpenes Isolated from Three Different Plectranthus Sensu Lato Species and Their Antiproliferative Activities against Gynecological and Glioblastoma Cancer Cells}, url = {https://m2.mtmt.hu/api/publication/34779108}, author = {Gáborová, Mária and Vágvölgyi, Máté and Tayeb, Bizhar Ahmed and Minorics, Renáta and Zupkó, István and Jurček, Ondřej and Béni, Szabolcs and Kubínová, Renata and Balogh, György Tibor and Hunyadi, Attila}, doi = {10.1021/acsomega.4c00800}, journal-iso = {ACS OMEGA}, journal = {ACS OMEGA}, volume = {9}, unique-id = {34779108}, issn = {2470-1343}, year = {2024}, eissn = {2470-1343}, pages = {18495-18504}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Tayeb, Bizhar Ahmed/0000-0001-5197-564X; Minorics, Renáta/0000-0001-9685-813X; Zupkó, István/0000-0003-3243-5300; Béni, Szabolcs/0000-0001-7056-6825; Balogh, György Tibor/0000-0003-3347-1880; Hunyadi, Attila/0000-0003-0074-3472} } @article{MTMT:34691003, title = {17-Oxime ethers of oxidized ecdysteroid derivatives modulate oxidative stress in human brain endothelial cells and dose-dependently might protect or damage the blood-brain barrier}, url = {https://m2.mtmt.hu/api/publication/34691003}, author = {Vágvölgyi, Máté and Laczkó, Dávid and Santa Maria, Anaraquel and Vigh, Judit Piroska and Walter, Fruzsina and Berkecz, Róbert and Deli, Mária Anna and Tóth, Gábor and Hunyadi, Attila}, doi = {10.1371/journal.pone.0290526}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {19}, unique-id = {34691003}, issn = {1932-6203}, abstract = {20-Hydroxyecdysone and several of its oxidized derivatives exert cytoprotective effect in mammals including humans. Inspired by this bioactivity of ecdysteroids, in the current study it was our aim to prepare a set of sidechain-modified derivatives and to evaluate their potential to protect the blood-brain barrier (BBB) from oxidative stress. Six novel ecdysteroids, including an oxime and five oxime ethers, were obtained through regioselective synthesis from a sidechain-cleaved calonysterone derivative 2 and fully characterized by comprehensive NMR techniques revealing their complete 1 H and 13 C signal assignments. Surprisingly, several compounds sensitized hCMEC/D3 brain microvascular endothelial cells to tert -butyl hydroperoxide (tBHP)-induced oxidative damage as recorded by impedance measurements. Compound 8 , containing a benzyloxime ether moiety in its sidechain, was the only one that exerted a protective effect at a higher, 10 μM concentration, while at lower (10 nM– 1 μM) concentrations it promoted tBHP-induced cellular damage. Brain endothelial cells were protected from tBHP-induced barrier integrity decrease by treatment with 10 μM of compound 8 , which also mitigated the intracellular reactive oxygen species production elevated by tBHP. Based on our results, 17-oxime ethers of oxidized ecdysteroids modulate oxidative stress of the BBB in a way that may point towards unexpected toxicity. Further studies are needed to evaluate any possible risk connected to dietary ecdysteroid consumption and CNS pathologies in which BBB damage plays an important role.}, year = {2024}, eissn = {1932-6203}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Santa Maria, Anaraquel/0000-0003-3505-5477; Walter, Fruzsina/0000-0001-8145-2823; Berkecz, Róbert/0000-0002-9076-2177; Deli, Mária Anna/0000-0001-6084-6524; Hunyadi, Attila/0000-0003-0074-3472} } @CONFERENCE{MTMT:33963548, title = {Antitrypanosomal activity of natural and semi-synthetic ecdysteroids}, url = {https://m2.mtmt.hu/api/publication/33963548}, author = {Háznagy, Márton Benedek and Vágvölgyi, Máté and Krishnan, Sandhya R. and Gertsch, Jürg and Hunyadi, Attila}, booktitle = {4th Symposium of Young Researchers on Pharmacognosy}, doi = {10.14232/syrmpnpr.2023.10}, unique-id = {33963548}, year = {2023}, pages = {16-16}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Gertsch, Jürg/0000-0003-0978-1555; Hunyadi, Attila/0000-0003-0074-3472} } @CONFERENCE{MTMT:33963351, title = {Structure elucidation of diterpenoids isolated from three Plectranthus sensu lato species}, url = {https://m2.mtmt.hu/api/publication/33963351}, author = {Gáborová, Mária and Vágvölgyi, Máté and Hunyadi, Attila and Béni, Szabolcs and Kubínová, Renata}, booktitle = {4th Symposium of Young Researchers on Pharmacognosy}, doi = {10.14232/syrmpnpr.2023.2}, unique-id = {33963351}, year = {2023}, pages = {8-8}, orcid-numbers = {Gáborová, Mária/0000-0002-2928-1219; Vágvölgyi, Máté/0000-0002-2233-9422; Hunyadi, Attila/0000-0003-0074-3472; Béni, Szabolcs/0000-0001-7056-6825} } @article{MTMT:34103969, title = {Anticancer Meroterpenoids from Centrapalus pauciflorus leaves: Chromone- and 2,4-Chromadione-Monoterpene Derivatives}, url = {https://m2.mtmt.hu/api/publication/34103969}, author = {Krstić, Gordana and Saidu, Muhammad Bello and Barta, Anita and Vágvölgyi, Máté and Ali, Hazhmat and Zupkó, István and Berkecz, Róbert and Gallah, Umar Shehu and Rédei, Dóra and Hohmann, Judit}, doi = {10.1021/acsomega.3c03884}, journal-iso = {ACS OMEGA}, journal = {ACS OMEGA}, volume = {8}, unique-id = {34103969}, issn = {2470-1343}, year = {2023}, eissn = {2470-1343}, pages = {31389-31398}, orcid-numbers = {Krstić, Gordana/0000-0001-6945-6178; Vágvölgyi, Máté/0000-0002-2233-9422; Zupkó, István/0000-0003-3243-5300; Berkecz, Róbert/0000-0002-9076-2177; Rédei, Dóra/0000-0002-5013-247X; Hohmann, Judit/0000-0002-2887-6392} } @{MTMT:33552107, title = {Effects of calonysterone and 20-hydroxyecdysone in the obese rat model}, url = {https://m2.mtmt.hu/api/publication/33552107}, author = {Alaa, A.M. Osman and Laczkó, Dávid and Vágvölgyi, Máté and Hunyadi, Attila and Ducza, Eszter}, booktitle = {Natural vs. Artificial Networks: The Usefulness of the Concept in Health, Life, and Technical Sciences}, unique-id = {33552107}, abstract = {Obesity is a global pandemic and a serious health problem. Phytoecdysteroids, the polyhydroxylated steroids of the C-27 cholesterol skeleton, has a wide variety of beneficial effects including anabolic, antidiabetic, and anti-obesity. Our studies aimed to investigate the effects of two phytoecdysteroids derivatives (calonysterone and 20-hydroxyecdysone) on body weight, blood glucose, antioxidant capacity, and the level of adipokines, the expression of cytokines of lowgrade inflammation, and the epigenetic modification in the obese rat model. 48 male Sprague Dawley rats, aged 5-6 weeks were divided into 8 groups (6 rats/group) and fed high fat and high sugar diet (HFHSD) or a normal diet. Rats received daily oral treatments for 12 weeks of calonysterone, 20-hydroxyecdysone, vehicle, or no treatment. Body weight, caloric intake, and plasma glucose during the glucose tolerance test were measured at baseline and during the experiment. We measured the liver levels of catalase (CAT) and superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and global DNA methylation using colorimetric assay kits. The hepatic expression of leptin, adiponectin, TNF α, IL-6, IL2, and IL10 mRNA and protein were measured using RT-PCR and western blot, respectively. CAT levels were increased by both treatments, while T-AOC increased by 20-hydroxyecdysone. Obese rats showed increased expression of leptin mRNA and protein levels that were reduced by calonysterone. 20-hydroxyecdysone further increased leptin mRNA but reduced protein levels. The levels of global DNA methylation were increased by both treatments. Calonysterone may have anti-obesity effects and both treatments may have antidiabetic and antioxidant effects. Our preliminary screening of the effects of treatments on markers of inflammation showed significant differences in the levels of IL-6 and IL10 between normal and obese rats. We suppose these phytoecdysteroids may affect epigenetic modification at specific gene levels too.}, year = {2022}, pages = {83-84}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Hunyadi, Attila/0000-0003-0074-3472} } @article{MTMT:33686924, title = {Antitrypanosomal activity of semisyntetic enone-type derivatives}, url = {https://m2.mtmt.hu/api/publication/33686924}, author = {Háznagy, Márton Benedek and Vágvölgyi, Máté and Krishnan, S R and Gertsch, J and Hunyadi, Attila}, doi = {10.1055/s-0042-1759246}, journal-iso = {PLANTA MED}, journal = {PLANTA MEDICA: NATURAL PRODUCTS AND MEDICINAL PLANT RESEARCH}, volume = {88}, unique-id = {33686924}, issn = {0032-0943}, year = {2022}, eissn = {1439-0221}, pages = {1534}, orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Hunyadi, Attila/0000-0003-0074-3472} } @article{MTMT:33041582, title = {C20-nor-Abietane and Three Abietane Diterpenoids from Plectranthus mutabilis Leaves as P-Glycoprotein Modulators}, url = {https://m2.mtmt.hu/api/publication/33041582}, author = {Ntungwe, Epole N. and Stojanov, Sofija Jovanović and Duarte, Noélia M. and Candeias, Nuno R. and Díaz-Lanza, Ana M. and Vágvölgyi, Máté and Hunyadi, Attila and Pešić, Milica and Rijo, Patrícia}, doi = {10.1021/acsmedchemlett.1c00711}, journal-iso = {ACS MED CHEM LETT}, journal = {ACS MEDICINAL CHEMISTRY LETTERS}, volume = {13}, unique-id = {33041582}, issn = {1948-5875}, year = {2022}, pages = {674-680}, orcid-numbers = {Candeias, Nuno R./0000-0003-2414-9064; Vágvölgyi, Máté/0000-0002-2233-9422; Hunyadi, Attila/0000-0003-0074-3472; Pešić, Milica/0000-0002-9045-8239; Rijo, Patrícia/0000-0001-7992-8343} } @article{MTMT:32765771, title = {Triterpenes and Phenolic Compounds from Euphorbia deightonii with Antiviral Activity against Herpes Simplex Virus Type-2}, url = {https://m2.mtmt.hu/api/publication/32765771}, author = {Saidu, Muhammad Bello and Kúsz, Norbert and Tsai, Yu-Chi and Vágvölgyi, Máté and Berkecz, Róbert and Kókai, Dávid and Burián, Katalin and Hohmann, Judit and Rédei, Dóra}, doi = {10.3390/plants11060764}, journal-iso = {PLANTS-BASEL}, journal = {PLANTS-BASEL}, volume = {11}, unique-id = {32765771}, abstract = {Two undescribed compounds, 3 beta,7 beta-dihydroxy-24-methylenelanosta-8-ene-11-one (1) and neolignane deightonin (4) were isolated from the aerial parts of Euphorbia deightonii Croizat together with six known compounds, namely, kansenone (2), euphorbol-7-one (3), dehydrodiconiferyl diacetate (5), marylaurencinol D (6), scoparon (7), and 3,4,3'-tri-O-methylellagic acid (8). The structures of the isolated compounds were determined by HRESIMS, 1D (H-1, C-13 JMOD) and 2D NMR (HSQC, HMBC, H-1-H-1 COSY, NOESY) spectroscopic analysis, and by comparison of the assignments with literature data. The anti-herpes simplex virus type-2 activity of the isolated compounds were investigated by qRT-PCR assay on Vero cells after determining cytotoxic concentration 50% (CC50). Compounds 1, 3, 4, and 7 exhibited inhibitory effects with respective IC50 values of 7.05, 2.42, 11.73, and 0.032 mu M. Scoparon (7) showed the strongest anti-HSV activity with a selectivity index of 10.93.}, year = {2022}, eissn = {2223-7747}, orcid-numbers = {Kúsz, Norbert/0000-0002-9973-6442; Vágvölgyi, Máté/0000-0002-2233-9422; Berkecz, Róbert/0000-0002-9076-2177; Burián, Katalin/0000-0003-1300-2374; Hohmann, Judit/0000-0002-2887-6392; Rédei, Dóra/0000-0002-5013-247X} } @article{MTMT:33538161, title = {Semi-Synthetic Ecdysteroid 6-Oxime Derivatives of 20-Hydroxyecdysone Possess Anti-Cryptococcal Activity}, url = {https://m2.mtmt.hu/api/publication/33538161}, author = {Szerencsés, Bettina and Vörös, Mónika and Bagi, Kristóf and Háznagy, Márton Benedek and Hunyadi, Attila and Vágvölgyi, Csaba and Pfeiffer, Ilona and Vágvölgyi, Máté}, doi = {10.3390/microbiolres13040071}, journal-iso = {MICROBIOL RES-ITALY}, journal = {MICROBIOLOGY RESEARCH}, volume = {13}, unique-id = {33538161}, issn = {2036-7473}, abstract = {Cryptococcosis, a life-threatening fungal infection, frequently occurs in patients suffering from AIDS. The treatment of the disease is hampered by the limited number of the effective drugs and the increasing resistance; therefore, to find new active substances is needed. As meningitis is the most serious infection affecting the AIDS patients, effective drugs have to be capable of entering to the central nervous system. Ecdysteroids are natural bioactive molecules with considerable anabolic activity and without toxic side effects on humans. The aim of this work was to study the anti-cryptococcal activity of a natural ecdysteroid, 20E, and its three semi-synthetic derivatives obtained by structural modification of the original molecule. We established the minimum inhibitory concentration of the compounds with microdilution method and demonstrated their fungicidal activity by flow cytometry and cultivation of the drug-treated cells. The interaction of the compounds with each other and efflux transporter inhibitors was assessed by checkerboard titration method. Two derivatives, 20E-EOx and 20E-ZOx, inhibited the growth of Cryptococcus neoformans with minimum inhibitory concentration 2 mg/mL and 1 mg/mL, respectively; both compounds possess fungicidal effect. A combination of the ecdysteroids with each other and verapamil resulted in additive interaction. This study confirmed that structural modification of an originally non-antimicrobial molecule can enhance its effectiveness.}, year = {2022}, eissn = {2036-7481}, pages = {985-994}, orcid-numbers = {Vörös, Mónika/0000-0003-4236-3929; Hunyadi, Attila/0000-0003-0074-3472; Vágvölgyi, Csaba/0000-0003-0009-7773; Pfeiffer, Ilona/0000-0003-0680-7596; Vágvölgyi, Máté/0000-0002-2233-9422} }