https://m2.mtmt.hu/ hun 2026-10-25 08:59 JournalArticle 37528373 VALIDATED true 0 false 2026-10-24T06:43:43.426+0000 2026-10-19T02:32:11.946+0000 2026-09-18T09:49:10.748+0000 true 10072271 Andódy Katalin /api/admin/10072271 Admin true 2026-10-24T06:42:33.947+0000 2026-10-19T02:32:11.020+0000 true 521 Szuper Admin /api/admin/521 Admin true false true true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Wong, Lok Hang Population balance modelling case studies for protein crystallisation: Workflows addressing impurities and heterogeneous seeding true true /api/journal/971 REVIEWED true false 971 false 971 true 0263-8762 1744-3563 Journal FOREIGN 232 544 565 544 22 2026 In this study, a population balance modelling (PBM) workflow for protein crystallisation is introduced and applied to two case studies, modelling lysozyme crystallisation in the presence of protein impurities (thaumatin and bovine serum albumin (BSA)) and heterogeneous silica seeds. The workflow systematically applies engineering judgement to identify phenomena (nucleation, growth, and enhancement/inhibition by foreign species), incorporates linear and exponential empirical adjustments to traditional kinetic models, and employs sequential kinetic parameter estimation to quantify phenomena. Across the two studies, workflow applicability was demonstrated for different crystalliser configurations (static/orbital shaking), buffer systems, foreign species, and scale-up (1-5 mL), whilst also overcoming experimental and solubility data limitations often encountered in high-cost crystallisation experiments. Although some calibrated parameters were uncertain with the 95% t-value smaller than the reference (attributed to a need for larger datasets), the workflow not only increased system knowledge but also revealed a critical knowledge gap: the complex, protein impurity-dependent behaviour of ternary lysozyme-silica-impurity systems, which traditional kinetic models proved unable to capture. The proposed workflow is broadly applicable to other peptides, proteins, or even small molecules, positioning it as a potential method for accelerating process design and understanding. true 2026 true true true false false false NONE 2026-10-24 false 0 0 0 0 0 0 0 0 0 0 0 0 6 6 Q2 A false false false false 2026-12-17T12:41:27.596+0000 14 21 0 3 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 141773347 /api/authorship/141773347 1 0.33333334 true false false Wong Lok Hang true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 141773348 /api/authorship/141773348 2 0.33333334 false false false Mitchell Niall Anthony true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 141773349 /api/authorship/141773349 3 0.33333334 false true true Heng Jerry Yong Yew true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 33050009 /api/publicationidentifier/33050009 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1016/j.cherd.2026.06.049 https://doi.org/10.1016/j.cherd.2026.06.049 false true PublicationIdentifier 33050010 /api/publicationidentifier/33050010 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001831780200001 https://www.webofscience.com/wos/woscc/full-record/001831780200001 false true PublicationIdentifier 33050013 /api/publicationidentifier/33050013 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 105045385954 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105045385954 false true PublicationIdentifier 32571104 /api/publicationidentifier/32571104 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true https://linkinghub.elsevier.com/retrieve/pii/S0263876226004132 https://linkinghub.elsevier.com/retrieve/pii/S0263876226004132 false true Keyword 3621 /api/keyword/3621 true 3621 true Keyword 6349 /api/keyword/6349 true 6349 true Keyword 10949 /api/keyword/10949 true 10949 true Keyword 1007206 /api/keyword/1007206 true 1007206 true Keyword 1100797 /api/keyword/1100797 true 1100797 true Keyword 1127681 /api/keyword/1127681 true 1127681 true Keyword 1389350 /api/keyword/1389350 true 1389350 true Keyword 3099244 /api/keyword/3099244 true true MtaRating 11435030 /api/mtarating/11435030 RatingType 10031 /api/ratingtype/10031 X. 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Földtudományok Osztálya HAS Section of Earth Sciences (X.) true 70 true true true A true true SjrRating 11795432 /api/sjrrating/11795432 93 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1501 /api/classificationexternal/1501 true 1501 true Q2 FROM_LAST_YEAR true true /api/publication/37528373 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Wong Lok Hang </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mitchell Niall Anthony </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Heng Jerry Yong Yew ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;37528373" target="_blank">Population balance modelling case studies for protein crystallisation: Workflows addressing impurities and heterogeneous seeding</a></div> <div> <span class="journal-title">CHEMICAL ENGINEERING RESEARCH AND DESIGN</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0263-8762">0263-8762</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1744-3563">1744-3563</a>)</span>: <span class="journal-volume">232</span> <span class="page"> pp 544-565 </span> <span class="year">(2026)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1016/j.cherd.2026.06.049" target="_blank" href="https://doi.org/10.1016/j.cherd.2026.06.049"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001831780200001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001831780200001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105045385954" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105045385954"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://linkinghub.elsevier.com/retrieve/pii/S0263876226004132" target="_blank" href="https://linkinghub.elsevier.com/retrieve/pii/S0263876226004132"> Egyéb URL </a> </span> </span> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;37528373&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 6 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 37528373</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS TAGGED</span> </div> <div class="lastModified">Utolsó módosítás: 2026.10.19. 04:32 Szuper Admin (admin) </div> </div></div>