https://m2.mtmt.hu/ eng 2026-07-26 10:36 JournalArticle 37120098 APPROVED true 0 false 2026-07-15T11:26:07.627+0000 2026-05-14T07:27:30.765+0000 2026-05-11T20:30:55.831+0000 true 10082377 Mogyoróssy Sándor Lajos /api/author/10082377 Author true 2026-05-14T07:27:31.191+0000 2026-07-01T06:24:07.666+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Mogyoróssy, Sándor Clinical Relevance and Methodological Comparison of Anti-MDA5 Antibody Detection: A Five-Year Retrospective and Exploratory Pilot Study true true /api/journal/10018879 REVIEWED true false 10018879 false 10018879 true 2218-273X 2218-273X Journal FOREIGN 16 5 698 698 2026 Anti-melanoma differentiation-associated gene 5 (anti-MDA5) antibodies are critical biomarkers in myositis, associated with distinct clinical features and prognosis. This study aimed to evaluate the proportion of anti-MDA5 positivity and compare the diagnostic performance of local immunoblotting (IB) with gold-standard immunoprecipitation (IP). We performed a retrospective analysis of 3272 physician-requested anti-MDA5 IB determinations over a five-year period (2019–2023). A subsequent exploratory pilot study of ten Hungarian patients with myositis was conducted to compare IB results with radiolabeled protein IP. Confirmatory in-house enzyme-linked immunosorbent assay (ELISA) was used to distinguish between 140 kDa bands (anti-MDA5 vs. anti-NXP2). Indirect immunofluorescence (IIF) on HEp-2 cells was also evaluated. In the retrospective cohort, 3.7% (n = 121) of samples were non-negative. Among 64 borderline patients, only one (1.6%) had a definitive diagnosis of dermatomyositis (DM). Conversely, the proportion of confirmed myositis cases was notably higher among patients with strong positive IB results. In our exploratory cross-sectional pilot study, complete concordance between the two assays was observed for negative and strong positive results. Discrepancies were noted in borderline and weak positivity ranges, where anti-MDA5 was not detected by IP; instead, alternative autoantibodies were identified. The three IP-confirmed MDA5 positive samples were all validated by ELISA. The characteristic IIF cytoplasmic staining was identifiable in 2 out of 10 cases (20%). In our cohort, borderline IB cases were frequently potential false positives, highlighting the need for careful clinical evaluation. Borderline and weak results require clinical correlation or confirmatory testing to avoid misdiagnosis. true 2026 true true true false true false GOLD 2026-07-15 false http://www.mdpi.com/journal/biomolecules 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Q1 false false false false 2026-08-09T20:44:57.602+0000 Belgyógyászati Intézet (DE / ÁOK); Klinikai Immunológiai Tanszék (DE / ÁOK / BelgyI); Laboratóriumi Medicina Intézet (DE / ÁOK); Orvostudományi Doktori Iskola (DE / ODT); Reumatológiai Tanszék (DE / ÁOK / BelgyI) 17 31 5 19 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 137860943 /api/authorship/137860943 1 0.053 true false false Author 10082377 /api/author/10082377 Mogyoróssy Sándor Lajos true true Mogyoróssy Sándor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860944 /api/authorship/137860944 2 0.053 false false false Author 10078258 /api/author/10078258 Nagy Gábor true true Nagy Gábor 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/api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860949 /api/authorship/137860949 7 0.053 false false false Author 10030122 /api/author/10030122 Tarr Tünde true 10030122 true Tarr Tünde true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860950 /api/authorship/137860950 8 0.053 false false false Author 10038759 /api/author/10038759 Zöld Éva true 10038759 true Zöld Éva true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860951 /api/authorship/137860951 9 0.053 false false false Author 10010008 /api/author/10010008 Pfliegler György true 10010008 true Pfliegler György true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860952 /api/authorship/137860952 10 0.053 false false false Author 10043292 /api/author/10043292 Brúgós Boglárka Csilla true 10043292 true Brúgós Boglárka Csilla true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860953 /api/authorship/137860953 11 0.053 false false false Lu Hui true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860954 /api/authorship/137860954 12 0.053 false false false Tansley Sarah true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860955 /api/authorship/137860955 13 0.053 false false false Author 10009455 /api/author/10009455 Antal-Szalmás Péter true 10009455 true Antal-Szalmás Péter true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860956 /api/authorship/137860956 14 0.053 false false false Author 10099496 /api/author/10099496 Domján Andrea true true Domján Andrea true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860957 /api/authorship/137860957 15 0.053 false false false Author 10048806 /api/author/10048806 Szamosi Szilvia true 10048806 true Szamosi Szilvia true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860958 /api/authorship/137860958 16 0.053 false false false Author 10030837 /api/author/10030837 Szűcs Gabriella true 10030837 true Szűcs Gabriella true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860959 /api/authorship/137860959 17 0.053 false false false Author 10003464 /api/author/10003464 Szekanecz Zoltán true 10003464 true Szekanecz Zoltán true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860960 /api/authorship/137860960 18 0.053 false true false Author 10083539 /api/author/10083539 Horváth Ágnes true true Horváth Ágnes true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 137860961 /api/authorship/137860961 19 0.053 false true true Author 10047408 /api/author/10047408 Bodoki Levente true 10047408 true Bodoki Levente true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 31988343 /api/publicationidentifier/31988343 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.3390/biom16050698 https://doi.org/10.3390/biom16050698 false true PublicationIdentifier 31988344 /api/publicationidentifier/31988344 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true IDENTICAL https://www.mdpi.com/2218-273X/16/5/698 https://www.mdpi.com/2218-273X/16/5/698 false true SjrRating 11668926 /api/sjrrating/11668926 69 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1303 /api/classificationexternal/1303 true 1303 true Q1 FROM_LAST_YEAR true true /api/publication/37120098 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10082377"><a href="/gui2/?type=authors&mode=browse&sel=10082377" target="_blank">Mogyoróssy Sándor (<span class="authorship-author-name">Mogyoróssy Sándor Lajos</span> <span class="authorAux-mtmt"> Reumatológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék; <span title="University of Debrecen">UD</span>/<span title="Orvostudományi Doktori Tanács">ODT</span>/Doctoral School of Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10078258"><a href="/gui2/?type=authors&mode=browse&sel=10078258" target="_blank">Nagy Gábor (<span class="authorship-author-name">Nagy Gábor</span> <span class="authorAux-mtmt"> laboratóriumi diagnosztika</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/Department of Laboratory Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10017537"><a href="/gui2/?type=authors&mode=browse&sel=10017537" target="_blank">Griger Zoltán (<span class="authorship-author-name">Griger Zoltán</span> <span class="authorAux-mtmt"> Belgyógyászat, Immunológia, Sejtélettan</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Klinikai Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10046165"><a href="/gui2/?type=authors&mode=browse&sel=10046165" target="_blank">Nagy-Vincze Melinda (<span class="authorship-author-name">Nagy-Vincze Melinda</span> <span class="authorAux-mtmt"> orvostudomány</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Klinikai Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10074511"><a href="/gui2/?type=authors&mode=browse&sel=10074511" target="_blank">Bodoki Monika (<span class="authorship-author-name">Bodoki Monika</span> <span class="authorAux-mtmt"> molekuláris biológus, angol-magyar orvos- és eg...</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10088694"><a href="/gui2/?type=authors&mode=browse&sel=10088694" target="_blank">Csige Dóra (<span class="authorship-author-name">Csige Dóra</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10030122"><a href="/gui2/?type=authors&mode=browse&sel=10030122" target="_blank">Tarr Tünde (<span class="authorship-author-name">Tarr Tünde</span> <span class="authorAux-mtmt"> klinikai orvostudomány</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Klinikai Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10038759"><a href="/gui2/?type=authors&mode=browse&sel=10038759" target="_blank">Zöld Éva (<span class="authorship-author-name">Zöld Éva</span> <span class="authorAux-mtmt"> belgyógyászat, immunológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Klinikai Immunológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10010008"><a href="/gui2/?type=authors&mode=browse&sel=10010008" target="_blank">Pfliegler György (<span class="authorship-author-name">Pfliegler György</span> <span class="authorAux-mtmt"> Haematológia - haemostasis, ritka betegségek</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/Belgyógyászati Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10043292"><a href="/gui2/?type=authors&mode=browse&sel=10043292" target="_blank">Brúgós Boglárka Csilla (<span class="authorship-author-name">Brúgós Boglárka Csilla</span> <span class="authorAux-mtmt"> belgyógyászat, reumatológia, klinikai immunológ...</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/Belgyógyászati Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lu Hui </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Tansley Sarah </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10009455"><a href="/gui2/?type=authors&mode=browse&sel=10009455" target="_blank">Antal-Szalmás Péter (<span class="authorship-author-name">Antal-Szalmás Péter</span> <span class="authorAux-mtmt"> Immunológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/Department of Laboratory Medicine</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10099496"><a href="/gui2/?type=authors&mode=browse&sel=10099496" target="_blank">Domján Andrea (<span class="authorship-author-name">Domján Andrea</span> <span class="authorAux-mtmt"> Reumatológia, immunológia, ápolástan</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10048806"><a href="/gui2/?type=authors&mode=browse&sel=10048806" target="_blank">Szamosi Szilvia (<span class="authorship-author-name">Szamosi Szilvia</span> <span class="authorAux-mtmt"> belgyógyászat, reumatológia, klinikai immunológ...</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10030837"><a href="/gui2/?type=authors&mode=browse&sel=10030837" target="_blank">Szűcs Gabriella (<span class="authorship-author-name">Szűcs Gabriella</span> <span class="authorAux-mtmt"> Reumatológia, allergológia és klinikai immunoló...</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10003464"><a href="/gui2/?type=authors&mode=browse&sel=10003464" target="_blank">Szekanecz Zoltán (<span class="authorship-author-name">Szekanecz Zoltán</span> <span class="authorAux-mtmt"> Reumatológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10083539"><a href="/gui2/?type=authors&mode=browse&sel=10083539" target="_blank">Horváth Ágnes<sup>**</sup> (<span class="authorship-author-name">Horváth Ágnes</span> <span class="authorAux-mtmt"> reumatológia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10047408"><a href="/gui2/?type=authors&mode=browse&sel=10047408" target="_blank">Bodoki Levente ✉ (<span class="authorship-author-name">Bodoki Levente</span> <span class="authorAux-mtmt"> orvostudomány</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Medicine">Faculty of Medicine</span>/<span title="Belgyógyászati Intézet">BelgyI</span>/Reumatológiai Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;37120098" target="_blank">Clinical Relevance and Methodological Comparison of Anti-MDA5 Antibody Detection: A Five-Year Retrospective and Exploratory Pilot Study</a></div> <div> <span class="journal-title">BIOMOLECULES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/2218-273X">2218-273X</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/2218-273X">2218-273X</a>)</span>: <span class="journal-volume">16</span> <span class="journal-issue">5</span> <span class="page"> p. 698. </span> <span class="year">(2026)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.3390/biom16050698" target="_blank" href="https://doi.org/10.3390/biom16050698"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="https://www.mdpi.com/2218-273X/16/5/698" target="_blank" href="https://www.mdpi.com/2218-273X/16/5/698"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="mtid"><span class="long-pub-mtid">Publication: 37120098</span> | <span class="status-data status-APPROVED"> Published </span> Core | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">DOI XML</span> </div> <div class="lastModified">Last Modified: 2026.05.14. 09:27 Zsuzsa Reményiné Kállai (DE admin 4) </div> </div></div>