https://m2.mtmt.hu/ eng 2026-10-25 07:51 JournalArticle 36409789 VALIDATED true 0 false 2026-07-24T11:38:43.977+0000 2026-05-21T19:22:27.934+0000 2025-10-31T08:31:35.143+0000 true 10065463 Turek Márta /api/admin/10065463 Admin true 2026-05-26T13:06:48.420+0000 2025-12-18T15:32:46.984+0000 true 521 Szuper Admin /api/admin/521 Admin true Admin 10025544 /api/admin/10025544 Lévayné Deseő Katalin true 10025544 true 2026-05-20T07:50:31.394+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Layous, R Optical recordings of unitary synaptic connections reveal high and random local connectivity between CA3 pyramidal cells true true /api/journal/2707 REVIEWED true false 2707 false 2707 true 0270-6474 1529-2401 Journal FOREIGN 45 36 e0102252025 e0102252025 13 2025 The hippocampal CA3 region is thought to play crucial roles in episodic memory functions because of the extensive recurrent connections between CA3 pyramidal cells (CA3PCs). However, different methods provided contradicting observations about the synaptic connectivity between CA3PCs. Therefore, we estimated the connectivity rate between individual CA3PCs using a new approach that is not affected by the confounds of conventional methods. Specifically, we used voltage imaging with the Voltron sensor in acute slices from rats of either sex to test CA3PC connections by detecting spontaneous spiking and subthreshold responses in anatomically identified neurons. We detected 164 monosynaptic excitatory connections in 3078 tested CA3PC-CA3PC pairs. This 5.3% connectivity rate was much higher than that we observed with the theoretically more sensitive patch clamp method in similar experimental conditions, but it remained below the anatomically observed number of CA3PC-CA3PC contacts. We verified that the imaged excitatory connections were mediated by AMPA receptors. Our results also showed that the recurrent connections did not enrich into preferred connectivity motifs and followed a distribution that was consistent with random connectivity in general. Moreover, voltage imaging revealed CA3PCs with distinct firing properties and somatic locations corresponding to previously established heterogeneity and showed that specific connectivity rules create preferred information routes among these subpopulations. Finally, we showed that there is at least one condition, influencing patch clamp recordings but not voltage imaging, that affects the observable functional connections between CA3PCs. Altogether, our results obtained with a new voltage imaging approach argue for high local connectivity rates between CA3PCs. 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false false false false 2026-01-29T08:33:31.015+0000 Celluláris Neurofarmakológia Kutatócsoport (HRN KOKI); MTA Kísérleti Orvostudományi Kutatóintézet 9 46 2 10 0 true 10025544 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 128332919 /api/authorship/128332919 1 0.1 true false Author 10101427 /api/author/10101427 Layous Róbert true true Layous R true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332920 /api/authorship/128332920 2 0.1 false false Author 73 /api/author/73 Bártfai Tamás true 73 true Tamas B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332921 /api/authorship/128332921 3 0.1 false false Author 10011504 /api/author/10011504 Mike Árpád true 10011504 true Mike A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332922 /api/authorship/128332922 4 0.1 false false Author 10040883 /api/author/10040883 Bodóné Sipos Eszter true 10040883 true Sipos E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332923 /api/authorship/128332923 5 0.1 false false Author 156 /api/author/156 Arszovszki Antónia true 156 true Arszovszki A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332924 /api/authorship/128332924 6 0.1 false false Author 10014148 /api/author/10014148 Brunner János true 10014148 true Brunner J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332925 /api/authorship/128332925 7 0.1 false false Szatai A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332926 /api/authorship/128332926 8 0.1 false false Author 10085824 /api/author/10085824 Farah Yaseen true true Yaseen F true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332927 /api/authorship/128332927 9 0.1 false false Author 10039582 /api/author/10039582 Andrási Tibor true 10039582 true Andrasi T true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 128332928 /api/authorship/128332928 10 0.1 false true Author 215 /api/author/215 Szabadics János true 215 true Szabadics J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 29701645 /api/publicationidentifier/29701645 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1523/JNEUROSCI.0102-25.2025 https://doi.org/10.1523/JNEUROSCI.0102-25.2025 false true PublicationIdentifier 30198781 /api/publicationidentifier/30198781 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS 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10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 40707357 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40707357&dopt=Abstract false true PublicationIdentifier 29701646 /api/publicationidentifier/29701646 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0102-25.2025 https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0102-25.2025 false true SjrRating 11607960 /api/sjrrating/11607960 20 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2801 /api/classificationexternal/2801 true 2801 true Q1 DIRECT true true /api/publication/36409789 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10101427"><a href="/gui2/?type=authors&mode=browse&sel=10101427" target="_blank">Layous R (<span class="authorship-author-name">Layous Róbert</span> <span class="authorAux-mtmt"> </span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="73"><a href="/gui2/?type=authors&mode=browse&sel=73" target="_blank">Tamas B (<span class="authorship-author-name">Bártfai Tamás</span> <span class="authorAux-mtmt"> Idegtudományok</span>) </a> </span> <span class="author-affil">MTA Kísérleti Orvostudományi Kutatóintézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011504"><a href="/gui2/?type=authors&mode=browse&sel=10011504" target="_blank">Mike A (<span class="authorship-author-name">Mike Árpád</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040883"><a href="/gui2/?type=authors&mode=browse&sel=10040883" target="_blank">Sipos E (<span class="authorship-author-name">Bodóné Sipos Eszter</span> <span class="authorAux-mtmt"> Idegtudomány</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="156"><a href="/gui2/?type=authors&mode=browse&sel=156" target="_blank">Arszovszki A (<span class="authorship-author-name">Arszovszki Antónia</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014148"><a href="/gui2/?type=authors&mode=browse&sel=10014148" target="_blank">Brunner J (<span class="authorship-author-name">Brunner János</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Szatai A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10085824"><a href="/gui2/?type=authors&mode=browse&sel=10085824" target="_blank">Yaseen F (<span class="authorship-author-name">Farah Yaseen</span> <span class="authorAux-mtmt"> cellular neurosceince</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10039582"><a href="/gui2/?type=authors&mode=browse&sel=10039582" target="_blank">Andrasi T (<span class="authorship-author-name">Andrási Tibor</span> <span class="authorAux-mtmt"> Elektrofiziológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="215"><a href="/gui2/?type=authors&mode=browse&sel=215" target="_blank">Szabadics J (<span class="authorship-author-name">Szabadics János</span> <span class="authorAux-mtmt"> Celluláris neurofarmakológia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Institute of Experimental Medicine">HUN-REN IEM</span>/Laboratory of Cellular Neuropharmacology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36409789" target="_blank">Optical recordings of unitary synaptic connections reveal high and random local connectivity between CA3 pyramidal cells</a></div> <div> <span class="journal-title">JOURNAL OF NEUROSCIENCE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0270-6474">0270-6474</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1529-2401">1529-2401</a>)</span>: <span class="journal-volume">45</span> <span class="journal-issue">36</span> <span class="page"> Paper e0102252025. 13 p. </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1523/JNEUROSCI.0102-25.2025" target="_blank" href="https://doi.org/10.1523/JNEUROSCI.0102-25.2025"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001574291100006" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001574291100006"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="243203" target="_blank" href="https://real.mtak.hu/243203"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105014993664" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105014993664"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="40707357" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40707357&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0102-25.2025" target="_blank" href="https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0102-25.2025"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 2</span> | Independent citation: 2 | Self citation: 0 | Unknown citation: 0 | Number of citations in WoS: 2 | Number of citations in Scopus:&nbsp;1 | WoS/Scopus assigned:&nbsp;2 | Number of citations with DOI:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;36409789&sort=publishedYear,desc&sort=title"> Number of cited publications: 16 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 36409789</span> | <span class="status-data status-VALIDATED"> Validated </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">WOS TAGGED</span> </div> <div class="funder"> Academy of Sciences(LP-2009-009), Union’s Horizon 2020(772452), Wellcome Trust(087497) </div> <div class="lastModified">Last Modified: 2026.05.21. 21:22 Katalin Lévayné Deseő (MTMT Központi admin) </div> <div class="lockedBy">Centrally managed 2026.05.20. 09:50 Katalin Lévayné Deseő (MTMT Központi admin) </div> </div></div>