https://m2.mtmt.hu/ eng 2026-03-05 09:20 BookChapter 36332642 ADMIN_APPROVED true 0 false 2026-03-02T10:20:28.166+0000 2026-02-12T09:56:40.620+0000 2025-09-15T14:07:19.490+0000 true 10020056 Solti Ádám /api/author/10020056 Author true 10020056 2026-02-12T09:56:35.645+0000 2026-02-12T09:56:35.645+0000 2026-02-12T09:56:40.625+0000 true 10019782 Csámer Iván /api/admin/10019782 Admin true 10019782 true false true 31 31 /api/publicationtype/31 PublicationType 4 true 31 BookChapter true 10000175 Abstract true 31 31 /api/publicationtype/31 PublicationType 4 true 31 BookChapter /api/subtype/10000175 Absztrakt / Kivonat SubType 453 true 10000175 true 1 /api/category/1 Category true 1 Francis, Michel Unraveling the role nitric oxide in plastidial iron allocation using bioinformatics and multielement clustering 16 16 2025 Understanding essential transition metal allocation in plants cells is pivotal to decode metal homeostasis in organelles. In that revealing microscopy X-Ray Fluorescence (μXRF) is a powerful technique, yet indeed generates a massive amount of data, requiring appropriate bioinformatics in dataset handling. To understand intracellular Fe allocation in nitric oxide signaling and autophagy compromised Arabidopsis thaliana mesophyll cells, we applied 20 μm thick cryosection samples. Dataset on the K edge signal of transition metal distribution were gained at ID21, European Synchrotron Radiation Facility, and analyzed using two computational approaches. First, sample was analyzed as composed of foreground and background layers, differentiated by intensity thresholds computing, and morphological operations were applied to improve the clarity of segmented regions. Focusing on background layer, away from organelles overlapping or noise, provided detailed structural interpretation of Fe-rich zones. The second approach applied k-means clustering, treating each pixel as a point in multidimensional element space, where each organelle occupied a distinct region in this space region based on its characteristic elemental composition and corresponding to its specific functions. Both approaches independently indicated that compromised lines show altered plastidial Fe allocation at senescence initiation. This agreement increases the confidence in the biological findings and in the robustness of each method. This work was supported by the grant K-146865 of NKFIH, Hungary. Á.S. was supported by the János Bolyai Scholarship of the Hungarian Academy of Sciences (BO-00113-23-8). We acknowledge the European Synchrotron Radiation Facility (ESRF) for provision of synchrotron radiation facilities under proposal number LS-3039. 2025 true true false true false NONE 2026-03-02 false 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 false false false false 2025-12-14T15:07:19.823+0000 Növényszervezettani Tanszék (ELTE / TTK / Bio_I); Növényélettani és Molekuláris Növénybiológiai T... (ELTE / TTK / Bio_I) 4 15 2 7 0 true true 10002 English /api/language/10002 Angol Language true 2 false /api/publication/36332640 true true 32 32 /api/publicationtype/32 PublicationType 6 true 32 Book Book of Abstracts PLANTMETALS - TRACE METAL METABOLISM IN PLANTS Conference 2025 false 36332640 false true true true false Book 2025 true 1 /api/category/1 Category true 1 false Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 127813985 /api/authorship/127813985 1 0.14285715 true false Francis Michel true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813986 /api/authorship/127813986 2 0.14285715 false false Author 10071444 /api/author/10071444 Gracheva Maria true true Gracheva Maria true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813987 /api/authorship/127813987 3 0.143 false false Author 10076692 /api/author/10076692 Sági-Kazár Máté true true Sági-Kazár Máté true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813988 /api/authorship/127813988 4 0.143 false false Author 10012272 /api/author/10012272 Solymosi Katalin true 10012272 true Solymosi Katalin true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813989 /api/authorship/127813989 5 0.14285715 false false Colocho Hurtarte Luis Carlos true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813990 /api/authorship/127813990 6 0.14285715 false false Castillo-Michel Hiram true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 127813991 /api/authorship/127813991 7 0.143 false true Author 10020056 /api/author/10020056 Solti Ádám true 10020056 true Solti Ádám true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true /api/publication/36332642 <div class="BookChapter Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <div class="autype autype0"> <span class="author-name" >Francis Michel </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071444"><a href="/gui2/?type=authors&mode=browse&sel=10071444" target="_blank">Gracheva Maria (<span class="authorship-author-name">Gracheva Maria</span> <span class="authorAux-mtmt"> Mössbauer Spectroscopy</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076692"><a href="/gui2/?type=authors&mode=browse&sel=10076692" target="_blank">Sági-Kazár Máté (<span class="authorship-author-name">Sági-Kazár Máté</span> <span class="authorAux-mtmt"> Növénybiológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Plant Physiology and Molecular Plant Biology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012272"><a href="/gui2/?type=authors&mode=browse&sel=10012272" target="_blank">Solymosi Katalin (<span class="authorship-author-name">Solymosi Katalin</span> <span class="authorAux-mtmt"> növénybiológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of of Plant Anatomy</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Colocho Hurtarte Luis Carlos </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Castillo-Michel Hiram </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020056"><a href="/gui2/?type=authors&mode=browse&sel=10020056" target="_blank">Solti Ádám (<span class="authorship-author-name">Solti Ádám</span> <span class="authorAux-mtmt"> Növénybiológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Plant Physiology and Molecular Plant Biology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36332642" target="_blank">Unraveling the role nitric oxide in plastidial iron allocation using bioinformatics and multielement clustering</a></div> <div class="InBook"><div class="chapter-in">In:</div> <div class="authors"> <div class="autype autype-1"> <span class="author-name" >Küpper H </span> (eds.) </div> </div> <div class="booktitle"><a href="/gui2/?mode=browse&params=publication;36332640" target="_blank">Book of Abstracts PLANTMETALS - TRACE METAL METABOLISM IN PLANTS Conference 2025 </a></div> <div class="conference"> Conference: <span class="location">České Budějovice, Czech Republic <span class="conference-date">2025.09.09. - 2025.09.12.</span> </div> : <span class="page"> p. 16. </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English </span> <span class="identifiers"> </span> <div class="mtid"><span class="long-pub-mtid">Publication: 36332642</span> | <span class="status-data status-ADMIN_APPROVED"> Admin approved </span> <span class="long-book-mtid">Book: 36332640</span> Core | <span class="type-subtype">Conference paper ( Abstract ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData"></span> </div> <div class="lastModified">Last Modified: 2026.02.12. 10:56 Iván Csámer (ELTE TTK kari admin 5) </div> </div> </div>