https://m2.mtmt.hu/ eng 2026-10-23 13:36 JournalArticle 36197999 APPROVED true Zsófia E. Kálmán and András Czajlik have equal contribution to the work. 0 false 2026-10-15T12:06:27.726+0000 2026-03-06T14:15:17.694+0000 2025-06-19T06:52:01.598+0000 true 10019567 Sifter Viktória /api/admin/10019567 Admin true 10019567 2026-10-15T12:04:24.789+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Zsófia, E. Kálmán Structural Modeling and Dynamics of the Full-Length Homer1 Multimer true true /api/journal/3802 REVIEWED true false 3802 false 3802 true 0887-3585 1097-0134 Journal FOREIGN 94 4 947 960 947 14 2025 Homer proteins are modular scaffold molecules that constitute an integral part of the protein network within the postsynaptic density. Full-length Homer1 forms a large homotetramer via a long coiled coil region, and can interact with proline-rich target sequences with its globular EVH1 domain. Here we report an atomistic model of the Homer1 coiled coil region along with the NMR solution structure and backbone dynamics of its EVH1 domain, with implications for the organization of the full-length tetramer. Compared to the already available EVH1 structures, our NMR ensemble exhibits subtle differences, mostly in and around its partner binding region, suggesting the presence of ligand-induced conformational transitions. Molecular dynamics simulations of the long coiled coil reveal distinct regions with different stability and flexibility, with the N-terminal part of the coiled coil exhibiting the largest motions. Interestingly, this segment is highly conserved, pointing to the functional relevance of the observed dynamical features. Our results indicate previously unexplored aspects of the flexibility of the full-length Homer1 tetramer that might contribute to the dynamic rearrangements of the postsynaptic protein network linked to its functional transitions. 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Kálmán (<span class="authorship-author-name">Kálmán Zsófia Etelka</span> <span class="authorAux-mtmt"> Fehérje bioinformatika</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10017084"><a href="/gui2/?type=authors&mode=browse&sel=10017084" target="_blank">András Czajlik<sup>*</sup> (<span class="authorship-author-name">Czajlik András</span> <span class="authorAux-mtmt"> Szerkezeti kémia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/<span title="Biokémiai és Molekuláris Biológiai Intézet">BMBI</span>/Biokémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Brigitta Maruzs </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076788"><a href="/gui2/?type=authors&mode=browse&sel=10076788" target="_blank">Fanni Farkas (<span class="authorship-author-name">Farkas Fanni</span> <span class="authorAux-mtmt"> Szerkezeti biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >István Pap </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Csilla Homonnay </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Tomas Klumpler </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10007156"><a href="/gui2/?type=authors&mode=browse&sel=10007156" target="_blank">Gyula Batta (<span class="authorship-author-name">Batta Gyula</span> <span class="authorAux-mtmt"> NMR spektroszkópia</span>) </a> </span> <span class="author-affil"><span title="University of Debrecen">UD</span>/<span title="Faculty of Science and Technology">Faculty of Science and Technology</span>/<span title="Institute of Chemistry">IChem</span>/Department of Organic Chemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001271"><a href="/gui2/?type=authors&mode=browse&sel=10001271" target="_blank">Zoltán Gáspári (<span class="authorship-author-name">Gáspári Zoltán</span> <span class="authorAux-mtmt"> Fehérje NMR, szerkezeti bioinformatika</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/Információs Technológiai és Bionikai Kar</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026672"><a href="/gui2/?type=authors&mode=browse&sel=10026672" target="_blank">Bálint Péterfia ✉ (<span class="authorship-author-name">Péterfia Bálint</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/Információs Technológiai és Bionikai Kar</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36197999" target="_blank">Structural Modeling and Dynamics of the Full-Length Homer1 Multimer</a></div> <div> <span class="journal-title">PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0887-3585">0887-3585</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1097-0134">1097-0134</a>)</span>: <span class="journal-volume">94</span> <span class="journal-issue">4</span> <span class="page"> pp 947-960 </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1002/prot.70091" target="_blank" href="https://doi.org/10.1002/prot.70091"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001619575800001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001619575800001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="405369" target="_blank" href="http://hdl.handle.net/2437/405369"> DEA </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105022635167" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105022635167"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="41267651" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=41267651&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Genetics&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Structural Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 1</span> | Independent citation: 0 | Self citation: 1 | Unknown citation: 0 | Number of citations in WoS: 1 | Number of citations in Scopus:&nbsp;1 | WoS/Scopus assigned:&nbsp;1 | Number of citations with DOI:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;36197999&sort=publishedYear,desc&sort=title"> Number of cited publications: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 36197999</span> | <span class="status-data status-APPROVED"> Published </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">WoS-XML</span> </div> <div class="funder"> (137947), Thematic Excellence Program(TKP2021-EGA-42), (2021-4.1.2-NEMZ_KI-2022-00027), (GINOP-2.3.2-15-2016-00008) Funder: Ministry of Human Capacities, GINOP(GINOP-2.3.3-15-2016-00004), (Digital Government Development and Project Management Ltd.) Funder: DKF </div> <div class="lastModified">Last Modified: 2026.03.06. 15:15 Mariann Szulyákné Kovács (DE/TTK admin 5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Zsófia E. Kálmán and András Czajlik have equal contribution to the work.</pre> </div></div>