https://m2.mtmt.hu/ hun 2026-09-22 18:19 JournalArticle 36183268 VALIDATED true Funding Agency and Grant Number: Hungarian National Research, Development, and Innovation Office (NKFIH) [K146906]; National Research, Development, and Innovation Fund by the Ministry of Innovation and Technology of Hungary [TKP2021-EGA-24, TKP2021-EGA]; University Research Scholarship Programme of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [2024-2.1.1.-EKOP-2020-00004] Funding text: The authors acknowledge support from the Hungarian National Research, Development, and Innovation Office (NKFIH) (grants K146906 to PI) and TKP2021-EGA-24 from the National Research, Development, and Innovation Fund by the Ministry of Innovation and Technology of Hungary, financed under the TKP2021-EGA) funding scheme, and also supported by the 2024-2.1.1.-EKOP-2020-00004 University Research Scholarship Programme of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. 0 false 2026-08-10T20:25:35.634+0000 2026-05-27T09:31:37.483+0000 2025-06-08T18:07:23.188+0000 true 10026589 Tichy Rita /api/admin/10026589 Admin true 10026589 2026-05-20T09:38:07.297+0000 2025-07-24T10:58:44.756+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 Admin 10025544 /api/admin/10025544 Lévayné Deseő Katalin true 10025544 true 2026-06-09T18:53:34.342+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Vékony, Bálint Challenges in circulating miRNA analysis in adrenocortical tumors true true /api/journal/7357 REVIEWED true false 7357 false 7357 true 1351-0088 1479-6821 Journal FOREIGN 32 6 e250045 e250045 2025 The differentiation of benign and malignant adrenocortical tumors is of major clinical relevance. Circulating microRNAs (miRNAs), hold promise as blood-borne biomarkers of adrenocortical cancer (ACC). There are, however, many difficulties with their use including technical and biological standardization challenges. Our aim was to evaluate the interchangeability of quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) for measuring circulating miRNAs and to investigate whether K2- and K3-EDTA as anticoagulant influence the measurements. Blood samples were drawn simultaneously from 20 participants into K2- and K3-EDTA tubes. Three miRNAs shown to be associated with ACC (miR-483-5p, miR-210-3p, miR-21-5p) together with two controls (miR-16-5p, cel-miR-39-3p) were analyzed using RT-qPCR and dPCR. qPCR and dPCR results showed different correlations in K2- and K3-EDTA samples with K2 performing better regarding ΔCt values. Moreover, proportional biases relating to low or high miRNA expression between the two methods were observed. In qPCR measurements, K3-EDTA samples showed larger standard deviations, particularly for cel-miR-39. While raw Ct values differed between K2 and K3 EDTA only for miR-483-5p, ΔCt values showed statistically significant differences across all miRNAs except for miR-483-5p. dPCR results were not affected by the choice of the anticoagulant. In conclusion, this is the first study demonstrating that dPCR and qPCR results are not easily interchangeable for circulating miRNA, particularly for abundant or rare miRNAs, making cross-validation studies challenging. K2 and K3-EDTA could potentially influence qPCR outcomes, underscoring the need for standardized protocols. A consensus-based methodology could improve reproducibility, enhancing miRNA-based biomarker utility in adrenocortical tumor diagnostics. Funding 2055518 /api/funding/2055518 false true Funding 2055519 /api/funding/2055519 false true Funding 2055520 /api/funding/2055520 false true Funding 2055521 /api/funding/2055521 false true true true INTERNATIONAL 2025 true true true false true false NONE 2026-08-10 false 1 0 1 1 0 0 1 0 1 1 1 1 1 1 0 0 1 1 1 1 0 10 10 Folyóiratcikk Journal Article 24 0 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2026 0 1 1 1 1 Q1 false 10000070 false false false 2025-06-30T09:16:36.234+0000 Belgyógyászati és Onkológiai Klinika (SE / AOK / K); Endokrinológiai Tanszék (SE / AOK / K / BOK); Farmakológiai és Farmakoterápiás Intézet (SE / AOK / I); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); HUN-REN-SE Örökletes Daganatok Kutatócsoport (SE / AOK / I / LABMEDINT); Laboratóriumi Medicina Intézet (SE / AOK / I); Országos Onkológiai Intézet 8 42 7 8 0 true 10025544 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 126843986 /api/authorship/126843986 1 0.125 true false false Author 10084110 /api/author/10084110 Vékony Bálint true true Vékony Bálint true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843987 /api/authorship/126843987 2 0.125 true false false Author 10027574 /api/author/10027574 Nyírő Gábor true 10027574 true Nyirő Gábor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843988 /api/authorship/126843988 3 0.125 false false false Author 10020420 /api/author/10020420 Butz Henriett true 10020420 true Butz Henriett true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843989 /api/authorship/126843989 4 0.125 false false false Author 10095127 /api/author/10095127 Szeredás Bálint Kende true true Szeredás Bálint Kende true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843990 /api/authorship/126843990 5 0.125 false false false Author 10040905 /api/author/10040905 Tóth Viktória true 10040905 true Tóth Viktória true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843991 /api/authorship/126843991 6 0.125 false false false Author 10010125 /api/author/10010125 Ferdinandy Péter true 10010125 true Ferdinandy Peter true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843992 /api/authorship/126843992 7 0.125 false false false Author 10001006 /api/author/10001006 Patócs Attila Balázs true 10001006 true Patocs Attila true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126843993 /api/authorship/126843993 8 0.125 false true true Author 10000070 /api/author/10000070 Igaz Péter true 10000070 true Igaz Peter true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 29466403 /api/publicationidentifier/29466403 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1530/ERC-25-0045 https://doi.org/10.1530/ERC-25-0045 false true PublicationIdentifier 29645864 /api/publicationidentifier/29645864 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001529647700012 https://www.webofscience.com/wos/woscc/full-record/001529647700012 true true PublicationIdentifier 29540774 /api/publicationidentifier/29540774 PlainSource 3 /api/publicationsource/3 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https://erc.bioscientifica.com/view/journals/erc/32/6/ERC-25-0045.xml https://erc.bioscientifica.com/view/journals/erc/32/6/ERC-25-0045.xml false true PublicationIdentifier 32259419 /api/publicationidentifier/32259419 PlainSource 40 /api/publicationsource/40 PublicationSourceType 10006 /api/publicationsourcetype/10006 true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/pages/publications/105008502815?origin=resultslist https://www.scopus.com/pages/publications/105008502815?origin=resultslist false true Classification 12284 /api/classification/12284 true true SjrRating 11579968 /api/sjrrating/11579968 24 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1310 /api/classificationexternal/1310 true 1310 true Q1 DIRECT true true /api/publication/36183268 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10084110"><a href="/gui2/?type=authors&mode=browse&sel=10084110" target="_blank">Vékony Bálint (<span class="authorship-author-name">Vékony Bálint</span> <span class="authorAux-mtmt"> endokrinológia, molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Belgyógyászati és Onkológiai Klinika">BOK</span>/Endokrinológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027574"><a href="/gui2/?type=authors&mode=browse&sel=10027574" target="_blank">Nyirő Gábor<sup>*</sup> (<span class="authorship-author-name">Nyírő Gábor</span> <span class="authorAux-mtmt"> Molekuláris biológia-genetika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Belgyógyászati és Onkológiai Klinika">BOK</span>/Endokrinológiai Tanszék; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Laboratóriumi Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020420"><a href="/gui2/?type=authors&mode=browse&sel=10020420" target="_blank">Butz Henriett (<span class="authorship-author-name">Butz Henriett</span> <span class="authorAux-mtmt"> orvostudomány</span>) </a> </span> <span class="author-affil">Országos Onkológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Laboratóriumi Medicina Intézet">LABMEDINT</span>/HUN-REN-SE Örökletes Daganatok Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Laboratóriumi Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10095127"><a href="/gui2/?type=authors&mode=browse&sel=10095127" target="_blank">Szeredás Bálint Kende (<span class="authorship-author-name">Szeredás Bálint Kende</span> <span class="authorAux-mtmt"> Endokrin és neuroendokrin daganatok diagnosztik...</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Belgyógyászati és Onkológiai Klinika">BOK</span>/Endokrinológiai Tanszék; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040905"><a href="/gui2/?type=authors&mode=browse&sel=10040905" target="_blank">Tóth Viktória (<span class="authorship-author-name">Tóth Viktória</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10010125"><a href="/gui2/?type=authors&mode=browse&sel=10010125" target="_blank">Ferdinandy Peter (<span class="authorship-author-name">Ferdinandy Péter</span> <span class="authorAux-mtmt"> Farmakológia, molekuláris medicina</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Farmakológiai és Farmakoterápiás Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001006"><a href="/gui2/?type=authors&mode=browse&sel=10001006" target="_blank">Patocs Attila (<span class="authorship-author-name">Patócs Attila Balázs</span> <span class="authorAux-mtmt"> Orvostudomány</span>) </a> </span> <span class="author-affil">Országos Onkológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Laboratóriumi Medicina Intézet">LABMEDINT</span>/HUN-REN-SE Örökletes Daganatok Kutatócsoport; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Laboratóriumi Medicina Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000070"><a href="/gui2/?type=authors&mode=browse&sel=10000070" target="_blank">Igaz Peter ✉ (<span class="authorship-author-name">Igaz Péter</span> <span class="authorAux-mtmt"> belgyógyászat, endokrinológia, klinikai molekul...</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/<span title="Belgyógyászati és Onkológiai Klinika">BOK</span>/Endokrinológiai Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36183268" target="_blank">Challenges in circulating miRNA analysis in adrenocortical tumors</a></div> <div> <span class="journal-title">ENDOCRINE-RELATED CANCER</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1351-0088">1351-0088</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1479-6821">1479-6821</a>)</span>: <span class="journal-volume">32</span> <span class="journal-issue">6</span> <span class="page"> Paper e250045. </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1530/ERC-25-0045" target="_blank" href="https://doi.org/10.1530/ERC-25-0045"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001529647700012" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001529647700012"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105008502815" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105008502815"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="40472370" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40472370&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="https://erc.bioscientifica.com/view/journals/erc/32/6/ERC-25-0045.xml" target="_blank" href="https://erc.bioscientifica.com/view/journals/erc/32/6/ERC-25-0045.xml"> Egyéb URL </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/pages/publications/105008502815?origin=resultslist" target="_blank" href="https://www.scopus.com/pages/publications/105008502815?origin=resultslist"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Endocrinology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Endocrinology, Diabetes and Metabolism&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Oncology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cancer Research&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 1</span> | Független: 1 | Függő: 0 | Nem jelölt: 0 | Scopus jelölt:&nbsp;1 | WoS/Scopus jelölt:&nbsp;1 | DOI jelölt:&nbsp;1 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;36183268&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 10 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 36183268</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (K146906), (TKP2021-EGA-24), (TKP2021-EGA), (2024-2.1.1.-EKOP-2020-00004) </div> <div class="lastModified">Utolsó módosítás: 2026.05.27. 11:31 Szalóky-Siki Ágnes (SE_KK_Admin5_SZSA, admin) </div> <div class="lockedBy">Központi kezelésű 2026.06.09. 20:53 Lévayné Deseő Katalin (MTMT Központi admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: Hungarian National Research, Development, and Innovation Office (NKFIH) [K146906]; National Research, Development, and Innovation Fund by the Ministry of Innovation and Technology of Hungary [TKP2021-EGA-24, TKP2021-EGA]; University Research Scholarship Programme of the Ministry for Culture and Innovation from the source of the National Research, Development and...</pre> </div></div>