https://m2.mtmt.hu/ eng 2026-05-04 10:12 JournalArticle 36141720 ADMIN_APPROVED true Funding Agency and Grant Number: National Research, Development and Innovation Fund of Hungary [K138937]; Hungarian Academy of Sciences [NAP3.0 Program] [NAP2022-I-3/2022]; Eotvos Lorand University Excellence Fund [EKA 2022/045-P278-1]; DKOEP-23 Doctoral Excellence Program of the Ministry for Culture and Innovation from NRDI Fund; SOLEIL Synchrotron, France [20230777, 20231948, 20240797, 20241998]; National Research Foundation of Korea grant - Korean government [RS-2022-NR069719, RS-2021-NR057690]; KBSI fund [A412580, A423310, A439200, C512120, C523200, C539200]; National Research, Development and Innovation Fund of Hungary [K138937] Funding text: This work was supported by the National Research, Development and Innovation Fund of Hungary [grant #K138937], by the Hungarian Academy of Sciences [NAP3.0 Program NAP2022-I-3/2022] and by the Eotvos Lorand University Excellence Fund [EKA 2022/045-P278-1]. M.P.N. was supported by the DKOEP-23 Doctoral Excellence Program of the Ministry for Culture and Innovation from the source of the NRDI Fund. SRCD measurements were supported by SOLEIL Synchrotron, France (proposals 20230777, 20231948, 20240797, and 20241998). Y.-H.L. was supported by the National Research Foundation of Korea grant funded by the Korean government [RS-2022-NR069719, RS-2021-NR057690] and the KBSI fund [A412580, A423310, A439200, C512120, C523200, and C539200]. Funding to pay the Open Access publication charges for this article was provided by the National Research, Development and Innovation Fund of Hungary [grant # K138937]. 0 false 2026-04-30T13:32:05.842+0000 2025-09-17T09:45:30.327+0000 2025-05-14T17:13:15.165+0000 true 10071503 Szabó Regina /api/admin/10071503 Admin Regina Szabó (ELTE TTK admin 5) true 2026-04-30T13:25:45.051+0000 2025-09-17T09:45:30.585+0000 true 10071503 Szabó Regina /api/admin/10071503 Admin Regina Szabó (ELTE TTK admin 5) true true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Micsonai, András true true /api/journal/3431 REVIEWED NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 true false 3431 false 3431 true 0305-1048 1362-4962 Journal FOREIGN 53 W1 W73 W83 W73 2025 Circular dichroism (CD) spectroscopy is a widely used technique to characterize the secondary structure composition of proteins. We have developed the Beta Structure Selection (BeStSel) method (PNAS, 112, E3095), which solves the main problem of protein CD spectroscopy—namely, the spectral variability of β-structures. The BeStSel web server utilizes this method to provide tools to the community for CD spectrum analysis. BeStSel uniquely provides information on eight secondary structure components, including parallel β-structure and antiparallel β-sheets with three different twist groups. It outperforms all available methods in accuracy and information content, and is also able to predict protein folds down to the topology/homology level of the CATH classification. The algorithm has been further developed, and the accuracy of the estimation of the secondary structure elements is improved by 0.7% as an average on the reference dataset. A new module of the web server calculates protein stability from the thermal denaturation profile followed by CD. Secondary structure calculations of uploaded PDB and mmcif files support the experimental verification of MD simulations and AlphaFold models by CD spectroscopy. Well-proven modules for disorder–order classification and extinction coefficient calculation continue to work. The BeStSel server is freely accessible at https://bestsel.elte.hu. true 2025 true true true false false GOLD 2026-04-30 true https://doi.org/10.1093/nar/gkaf378 14 0 14 12 0 14 14 13 10 13 13 12 12 12 2 0 14 12 14 12 0 9 9 Folyóiratcikk Journal Article 24 14 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 9 8 9 8 2026 0 5 4 5 4 D1 false 10014881,10020440 false false false 2025-08-12T17:15:55.449+0000 Biokémiai Tanszék (ELTE / TTK / Bio_I); MTA-ELTE-NAP B Neuroimmunológiai Kutatócsoport (ELTE / TTK / Bio_I) 5 18 2 10 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 126521661 /api/authorship/126521661 Micsonai, András ✉ [Micsonai, András (biokémia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I) 1 0.1 true false true Author 10020440 /api/author/10020440 András Micsonai (biokémia) Micsonai András true 10020440 true Micsonai András true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521662 /api/authorship/126521662 Wien, Frank 2 0.1 false false false Wien Frank true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521663 /api/authorship/126521663 Murvai, Nikoletta [Murvai, Nikoletta (Molekuláris biológia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I) 3 0.1 false false false Author 10060353 /api/author/10060353 Nikoletta Murvai (Molekuláris biológia) Murvai Nikoletta true 10060353 true Murvai Nikoletta true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521664 /api/authorship/126521664 Nyiri, Márton Péter [Nyiri, Márton Péter (Biokémia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I) 4 0.1 false false false Author 10089499 /api/author/10089499 Márton Péter Nyiri (Biokémia) Nyiri Márton Péter true true Nyiri Márton Péter true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521665 /api/authorship/126521665 Balatoni, Bori 5 0.1 false false false Balatoni Bori true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521666 /api/authorship/126521666 Lee, Young-Ho 6 0.1 false false false Lee Young-Ho true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521667 /api/authorship/126521667 Molnár, Tamás [Molnár, Tamás (Biokémia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I); MTA-ELTE-NAP B Neuroimmunology Research Group (ELTE / ELU FoS / Bio_I) 7 0.1 false false false Author 10020510 /api/author/10020510 Tamás Molnár (Biokémia) Molnár Tamás true 10020510 true Molnár Tamás true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521668 /api/authorship/126521668 Goto, Yuji 8 0.1 false false false Goto Yuji true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521669 /api/authorship/126521669 Jamme, Frédéric 9 0.1 false false false Jamme Frédéric true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 126521670 /api/authorship/126521670 Kardos, József ✉ [Kardos, József (Biológia), author] Department of Biochemistry (ELTE / ELU FoS / Bio_I); MTA-ELTE-NAP B Neuroimmunology Research Group (ELTE / ELU FoS / Bio_I) 10 0.1 false true true Author 10014881 /api/author/10014881 József Kardos (Biológia) Kardos József true 10014881 true Kardos József true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PublicationIdentifier 29368002 /api/publicationidentifier/29368002 DOI: 10.1093/nar/gkaf378 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1093/nar/gkaf378 https://doi.org/10.1093/nar/gkaf378 false true PublicationIdentifier 29395152 /api/publicationidentifier/29395152 WoS: 001485714000001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001485714000001 https://www.webofscience.com/wos/woscc/full-record/001485714000001 false true PublicationIdentifier 29515093 /api/publicationidentifier/29515093 EDIT: 115828 SwordSource 92 /api/publicationsource/92 EDIT PublicationSourceType 10007 /api/publicationsourcetype/10007 Repozitórium true true true EDIT EDIT https://edit.elte.hu/xmlui/handle/10831/@@@ true true 92 true GOLD true 115828 https://edit.elte.hu/xmlui/handle/10831/115828 false true PublicationIdentifier 29722757 /api/publicationidentifier/29722757 Scopus: 105010747780 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 105010747780 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105010747780 false true PublicationIdentifier 29368004 /api/publicationidentifier/29368004 PubMed: 40357643 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 40357643 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40357643&dopt=Abstract false true PublicationIdentifier 29368003 /api/publicationidentifier/29368003 Other URL: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaf378/8129375 PlainSource 40 /api/publicationsource/40 Other URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaf378/8129375 https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaf378/8129375 false true SjrRating 11584367 /api/sjrrating/11584367 sjr:D1 (2025) Scopus - Genetics NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 5 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1311 /api/classificationexternal/1311 Scopus - Genetics true 1311 true D1 DIRECT true true Reference 65818889 /api/reference/65818889 1. 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BeStSel: analysis site for protein CD spectra—2025 update. (2025) NUCLEIC ACIDS RESEARCH 0305-1048 1362-4962 53 W1 W73-W83<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10020440"><a href="/gui2/?type=authors&mode=browse&sel=10020440" target="_blank">Micsonai András ✉ (<span class="authorship-author-name">Micsonai András</span> <span class="authorAux-mtmt"> biokémia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Wien Frank </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10060353"><a href="/gui2/?type=authors&mode=browse&sel=10060353" target="_blank">Murvai Nikoletta (<span class="authorship-author-name">Murvai Nikoletta</span> <span class="authorAux-mtmt"> Molekuláris biológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10089499"><a href="/gui2/?type=authors&mode=browse&sel=10089499" target="_blank">Nyiri Márton Péter (<span class="authorship-author-name">Nyiri Márton Péter</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Balatoni Bori </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lee Young-Ho </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020510"><a href="/gui2/?type=authors&mode=browse&sel=10020510" target="_blank">Molnár Tamás (<span class="authorship-author-name">Molnár Tamás</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry; <span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/MTA-ELTE-NAP B Neuroimmunology Research Group</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Goto Yuji </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Jamme Frédéric </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014881"><a href="/gui2/?type=authors&mode=browse&sel=10014881" target="_blank">Kardos József ✉ (<span class="authorship-author-name">Kardos József</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/Department of Biochemistry; <span title="Eötvös Loránd University">ELTE</span>/<span title="Faculty of Science">ELU FoS</span>/<span title="Institute of Biology">Bio_I</span>/MTA-ELTE-NAP B Neuroimmunology Research Group</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36141720" target="_blank">BeStSel: analysis site for protein CD spectra—2025 update</a></div> <div> <span class="journal-title">NUCLEIC ACIDS RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0305-1048">0305-1048</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1362-4962">1362-4962</a>)</span>: <span class="journal-volume">53</span> <span class="journal-issue">W1</span> <span class="page"> pp W73-W83 </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1093/nar/gkaf378" target="_blank" href="https://doi.org/10.1093/nar/gkaf378"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001485714000001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001485714000001"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="115828" target="_blank" href="https://edit.elte.hu/xmlui/handle/10831/115828"> EDIT </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105010747780" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105010747780"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="40357643" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40357643&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaf378/8129375" target="_blank" href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaf378/8129375"> Other URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Genetics&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 14</span> | Independent citation: 12 | Self citation: 2 | Unknown citation: 0 | Number of citations in WoS: 13 | Number of citations in Scopus:&nbsp;10 | WoS/Scopus assigned:&nbsp;13 | Number of citations with DOI:&nbsp;14 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;36141720&sort=publishedYear,desc&sort=title"> Number of cited publications: 9 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 36141720</span> | <span class="status-data status-ADMIN_APPROVED"> Admin approved </span> Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Last Modified: 2025.09.17. 11:45 Regina Szabó (ELTE TTK admin 5) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Funding Agency and Grant Number: National Research, Development and Innovation Fund of Hungary [K138937]; Hungarian Academy of Sciences [NAP3.0 Program] [NAP2022-I-3/2022]; Eotvos Lorand University Excellence Fund [EKA 2022/045-P278-1]; DKOEP-23 Doctoral Excellence Program of the Ministry for Culture and Innovation from NRDI Fund; SOLEIL Synchrotron, France [20230777, 20231948, 20240797, 202419...</pre> </div></div>