https://m2.mtmt.hu/ hun 2026-04-29 21:00 JournalArticle 36105462 VALIDATED true 0 false 2026-03-25T12:47:38.576+0000 2025-06-20T10:52:58.160+0000 2025-04-24T05:57:41.702+0000 true 10000091 Ilisz István /api/author/10000091 Author true 10000091 2025-12-31T03:08:26.892+0000 2025-05-20T11:29:54.182+0000 true 10058176 Nagyné Tari Emese /api/admin/10058176 Admin true 10058176 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Németi, Gábor High-Performance Liquid Chromatographic Separation of Stereoisomers of ß-Methyl-Substituted Unusual Amino Acids Utilizing Ion Exchangers Based on Cinchona Alkaloids true true /api/journal/10003252 REVIEWED true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 26 9 4004 4004 17 2025 Novel peptides based on common amino acid building blocks may serve as possible drug candidates; however, their flexible structures may require stabilization via the incorporation of conformational constraints. The insertion of unusual amino acids is a feasible option that may provide improved pharmacokinetic and pharmacodynamic properties of such peptide-type drugs. The stereochemical purity of these kinds of building blocks must be verified by an efficient separation technique, such as high-performance liquid chromatography. Here, we present and discuss the results of the stereoselective separation mechanism of ß-methylated phenylalanine (ß-MePhe), tyrosine (ß-MeTyr), 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (ß-MeTic), and cyclohexylalanine (ß-MeCha) together with non-methylated Phe, Tyr, Tic, and Cha applying Cinchona alkaloid-based chiral stationary phases (CSPs). The studied zwitterionic CSPs acting as ion exchangers provided optimal performance in the polar ionic mode when methanol or a mixture of methanol and acetonitrile was utilized as the mobile phase together with organic acid and base additives. It was found that the basicity of small amines applied as mobile phase additives did not directly influence the chromatographic ion exchange concept. However, the size of the amines and their concentration led to a reduced retention time following the principles of ion exchange chromatography. On the basis of a systematic study of the effects of the eluent composition on the chromatographic behavior, important structure–retention and enantioselectivity relationships could be revealed. Through a temperature study, it has become evident that the composition of the eluent and the structure of analytes markedly affect the thermodynamic properties. Funding 2052175 /api/funding/2052175 false true true 2025 true true true false true false GOLD 2026-03-25 true http://real.mtak.hu/235759 2 0 2 2 0 2 2 2 0 2 2 2 2 2 0 0 2 2 2 2 0 12 12 Folyóiratcikk Journal Article 24 2 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2026 0 2 2 2 2 D1 false 10000091 false false false 2025-05-19T11:29:53.886+0000 Biokémiai Intézet (HRN SZBK); Gyógyszeranalitikai Intézet (SZTE / GYTK); Gyógyszerkémiai Intézet (SZTE / GYTK) 7 28 3 10 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 126242512 /api/authorship/126242512 1 0.1 true false false Author 10077549 /api/author/10077549 Németi Gábor true true Németi Gábor true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242513 /api/authorship/126242513 2 0.1 false false false Author 10027474 /api/author/10027474 Berkecz Róbert true 10027474 true Berkecz Róbert true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242514 /api/authorship/126242514 3 0.1 false false false Author 10068206 /api/author/10068206 Ozsvár Dániel true true Ozsvár Dániel true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242515 /api/authorship/126242515 4 0.1 false false false Author 10002784 /api/author/10002784 Szakonyi Zsolt true 10002784 true Szakonyi Zsolt true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242516 /api/authorship/126242516 5 0.1 false false false Lindner Wolfgang true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242517 /api/authorship/126242517 6 0.1 false false false Misicka Aleksandra true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242518 /api/authorship/126242518 7 0.1 false false false Tymecka Dagmara true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242519 /api/authorship/126242519 8 0.1 false false false Author 10005935 /api/author/10005935 Tóth Géza true 10005935 true Tóth Géza true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242520 /api/authorship/126242520 9 0.1 false false false Author 10001707 /api/author/10001707 Péter Antal true 10001707 true Péter Antal true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 126242521 /api/authorship/126242521 10 0.1 false true true Author 10000091 /api/author/10000091 Ilisz István true 10000091 true Ilisz István true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 29271704 /api/publicationidentifier/29271704 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.3390/ijms26094004 https://doi.org/10.3390/ijms26094004 false true PublicationIdentifier 29503712 /api/publicationidentifier/29503712 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001487747100001 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/api/classification/12184 true true Classification 10354 /api/classification/10354 true true Classification 10002 /api/classification/10002 true true SjrRating 11609941 /api/sjrrating/11609941 18 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1605 /api/classificationexternal/1605 true 1605 true D1 FROM_LAST_YEAR true true /api/publication/36105462 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10077549"><a href="/gui2/?type=authors&mode=browse&sel=10077549" target="_blank">Németi Gábor (<span class="authorship-author-name">Németi Gábor</span> <span class="authorAux-mtmt"> Kromatográfia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszeranalitikai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10027474"><a href="/gui2/?type=authors&mode=browse&sel=10027474" target="_blank">Berkecz Róbert (<span class="authorship-author-name">Berkecz Róbert</span> <span class="authorAux-mtmt"> bioanalitika</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszeranalitikai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10068206"><a href="/gui2/?type=authors&mode=browse&sel=10068206" target="_blank">Ozsvár Dániel (<span class="authorship-author-name">Ozsvár Dániel</span> <span class="authorAux-mtmt"> kémia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszeranalitikai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002784"><a href="/gui2/?type=authors&mode=browse&sel=10002784" target="_blank">Szakonyi Zsolt (<span class="authorship-author-name">Szakonyi Zsolt</span> <span class="authorAux-mtmt"> szerves- és gyógyszerkémia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerkémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lindner Wolfgang </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Misicka Aleksandra </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Tymecka Dagmara </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10005935"><a href="/gui2/?type=authors&mode=browse&sel=10005935" target="_blank">Tóth Géza (<span class="authorship-author-name">Tóth Géza</span> <span class="authorAux-mtmt"> Radiokémia</span>) </a> </span> <span class="author-affil"><span title="HUN-REN Szegedi Biológiai Kutatóközpont">HRN SZBK</span>/Biokémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001707"><a href="/gui2/?type=authors&mode=browse&sel=10001707" target="_blank">Péter Antal (<span class="authorship-author-name">Péter Antal</span> <span class="authorAux-mtmt"> Kromatográfia</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszeranalitikai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000091"><a href="/gui2/?type=authors&mode=browse&sel=10000091" target="_blank">Ilisz István ✉ (<span class="authorship-author-name">Ilisz István</span> <span class="authorAux-mtmt"> Elválasztástechnika</span>) </a> </span> <span class="author-affil"><span title="Szegedi Tudományegyetem">SZTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszeranalitikai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;36105462" target="_blank">High-Performance Liquid Chromatographic Separation of Stereoisomers of ß-Methyl-Substituted Unusual Amino Acids Utilizing Ion Exchangers Based on Cinchona Alkaloids</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">26</span> <span class="journal-issue">9</span> <span class="page"> Paper 4004. 17 p. </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.3390/ijms26094004" target="_blank" href="https://doi.org/10.3390/ijms26094004"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001487747100001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001487747100001"> WoS </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="235759" target="_blank" href="http://real.mtak.hu/235759"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105004903646" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105004903646"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="40362243" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40362243&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="https://www.mdpi.com/1422-0067/26/9/4004" target="_blank" href="https://www.mdpi.com/1422-0067/26/9/4004"> Egyéb URL </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="36799" target="_blank" href="http://publicatio.bibl.u-szeged.hu/36799"> SZTE Publicatio </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Catalysis&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 2</span> | Független: 2 | Függő: 0 | Nem jelölt: 0 | WoS jelölt: 2 | WoS/Scopus jelölt:&nbsp;2 | DOI jelölt:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;36105462&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 12 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 36105462</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (TKP2021-EGA-32) Támogató: NKFIH </div> <div class="lastModified">Utolsó módosítás: 2025.06.20. 12:52 Patkósné Hanesz Andrea (SZBK-4-es admin) </div> </div></div>