The vitreous humor of the eye presents a crucial target for posterior segment therapy
due to its proximity to the retina and relatively easy accessibility. Although intravitreous
injections have long been the primary method for treating posterior segment disorders,
recent successes in non-invasive topical treatments have led to the exploration of
alternative administration routes. The objective of our study is to establish a platform
for a Parallel Artificial Membrane Permeability Assay (PAMPA) model that mimics the
posterior segment, modelling the permeation process of compounds applied topically
on the eye. The study demonstrates the acceptor-phase effects of sodium hyaluronate
and agar on passive permeability, while applying a previously published vitreous humor-mimetic
material. Physicochemical similarities of the acceptor phase media and freshly excised
porcine vitreous humor further support the applicability of the mimetic material,
based on viscosity and zeta potential measurement. As a result, a new concept is introduced
for measuring posterior segment permeability, with potential for future high throughput
screening applications.
