https://m2.mtmt.hu/ hun 2026-09-23 05:45 JournalArticle 35784145 APPROVED true Funding Agency and Grant Number: Ministry of Innovation and Technology, Hungary [TKP2021-EGA-42, 2024-2.1.1-EKOP]; National Research Development and Innovation Fund [TKP2021]; Pazmany Peter Catholic University Research Scholarship Program (Egyetemi Kutatoi Osztondij Program) Funding text: This work was supported by TKP2021-EGA-42 and 2024-2.1.1-EKOP grants, funded by the Ministry of Innovation and Technology, Hungary with support from the National Research Development and Innovation Fund under the TKP2021 program and Pazmany Peter Catholic University Research Scholarship Program (Egyetemi Kutatoi Osztondij Program). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 0 false 2026-09-18T10:48:36.974+0000 2026-09-17T12:25:16.957+0000 2025-02-24T17:41:18.568+0000 true 10080212 Kocsis Dorottya /api/author/10080212 Author Kocsis Dorottya (biológia) true 2026-09-18T10:46:46.064+0000 true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Kocsis, Dorottya true true /api/journal/10071051 REVIEWED BIOENGINEERING 2306-5354 true false 10071051 false 10071051 true 2306-5354 Journal FOREIGN 12 3 230 230 16 2025 In recent times, with the need for a reduction, refinement, and replacement of in vivo animal testing, there has been an increasing demand for the use of relevant in vitro human cell systems in drug development. There is also a great demand for the replacement of skin tissue in various wounds and burns. Furthermore, human skin cell-based in vitro systems can be used to investigate the side effects (toxicity and irritation) and tissue penetration of topical preparations. In this study, exploratory experiments were performed to produce artificial epidermis using two hydrogel scaffolds, alginate and GelMA C. The amount of keratinocytes added to the matrix (10–50–100 × 106/mL) and the duration of tissue maturation (fresh, 1–3–4 weeks) were optimized in an extensive study. The behavior and structure of the two hydrogels were functionally and morphologically assessed. The permeability order for caffeine in the tested barriers was the following: alginate > GelMA C > cellulose acetate membrane > rat skin. It was concluded that GelMA C matrix provides a more favorable environment for cell survival and tissue differentiation (as demonstrated by histology and immunohistochemistry) than alginate. The 3-week incubation and 50 × 106/mL cell number proved to be the most beneficial in the given system. This study provides data for the first time on the multifactorial optimization of two potential skin substitutes for tissue manufacturing. In order to use these results in tissue engineering, the fabricated artificial epidermis preparations must also be optimized for biocompatibility and from physical and mechanical point of views. Funding 2050405 /api/funding/2050405 Thematic Excellence Program(TKP2021-EGA-42) false true true false 2025 true true true false true false GOLD 2026-09-18 true https://publikacio.ppke.hu/id/eprint/2733 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 5 Q2 false 10080212 false false false 2025-02-24T07:52:33.415+0000 Belgyógyászati és Onkológiai Klinika (SE / AOK / K); Gyógyszertechnológiai Tanszék (DE / GYTK); Gyógyszerészeti Intézet (SE / GYTK); Információs Technológiai és Bionikai Kar (PPKE); Patológiai és Kísérleti Rákkutató Intézet (SE / AOK / I); Roska Tamás Műszaki és Természettudományi Dokto... (PPKE / ITK) 11 44 6 11 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 123492752 /api/authorship/123492752 Kocsis, Dorottya [Kocsis, Dorottya (biológia), szerző] Roska Tamás Műszaki és Természettudományi Dokto... (PPKE / ITK) 1 0.091 true false false Author 10080212 /api/author/10080212 Kocsis Dorottya (biológia) Kocsis Dorottya true true Kocsis Dorottya true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492753 /api/authorship/123492753 Sztankovics, Dániel [Sztankovics, Dániel (Onkológia), szerző] Patológiai és Kísérleti Rákkutató Intézet (SE / AOK / I) 2 0.091 false false false Author 10074525 /api/author/10074525 Sztankovics Dániel (Onkológia) Sztankovics Dániel true true Sztankovics Dániel true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492754 /api/authorship/123492754 Józsa, Liza [Józsa, Liza (gyógyszertechnológia), szerző] Gyógyszertechnológiai Tanszék (DE / GYTK) 3 0.091 false false false Author 10069255 /api/author/10069255 Józsa Liza (gyógyszertechnológia) Józsa Liza true true Józsa Liza true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492755 /api/authorship/123492755 Németh, Afrodité [Németh, Afrodité (biológia), szerző] Roska Tamás Műszaki és Természettudományi Dokto... (PPKE / ITK) 4 0.09090909 false false false Author 10080217 /api/author/10080217 Németh Afrodité (biológia) Németh Afrodité true true Németh Afrodité true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492756 /api/authorship/123492756 Garay, Tamás ✉ [Garay, Tamás (sejtbiológia, tum...), szerző] Információs Technológiai és Bionikai Kar (PPKE); Belgyógyászati és Onkológiai Klinika (SE / AOK / K) 5 0.091 false false true Author 10030126 /api/author/10030126 Garay Tamás (sejtbiológia, tumorbiológia) Garay Tamás true 10030126 true Garay Tamás true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492757 /api/authorship/123492757 Naszlady, Márton Bese ✉ [Naszlady, Márton Bese (Műszaki tudományok), szerző] Információs Technológiai és Bionikai Kar (PPKE) 6 0.091 false false true Author 10084103 /api/author/10084103 Naszlady Márton Bese (Műszaki tudományok) Naszlady Márton Bese true true Naszlady Márton Bese true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492758 /api/authorship/123492758 Lengyel, Miléna [Lengyel, Miléna (Gyógyszertechnológia), szerző] Gyógyszerészeti Intézet (SE / GYTK) 7 0.09090909 false false false Author 10020628 /api/author/10020628 Lengyel Miléna (Gyógyszertechnológia) Lengyel Miléna true 10020628 true Lengyel Miléna true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492759 /api/authorship/123492759 Vecsernyés, Miklós [Vecsernyés, Miklós (gyógyszerésztudomány), szerző] Gyógyszertechnológiai Tanszék (DE / GYTK) 8 0.091 false false false Author 10001742 /api/author/10001742 Vecsernyés Miklós (gyógyszerésztudomány) Vecsernyés Miklós true 10001742 true Vecsernyés Miklós true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492760 /api/authorship/123492760 Antal, István [Antal, István (Gyógyszertechnológia), szerző] Gyógyszerészeti Intézet (SE / GYTK) 9 0.091 false false false Author 10020626 /api/author/10020626 Antal István (Gyógyszertechnológia) Antal István true 10020626 true Antal István true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492761 /api/authorship/123492761 Sebestyén, Anna [Sebestyén, Anna (Tumorbiológia), szerző] Patológiai és Kísérleti Rákkutató Intézet (SE / AOK / I) 10 0.091 false false false Author 10000745 /api/author/10000745 Sebestyén Anna (Tumorbiológia) Sebestyén Anna true 10000745 true Sebestyén Anna true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 123492762 /api/authorship/123492762 Erdő, Franciska ✉ [Erdő, Franciska (biológia, neurode...), szerző] Információs Technológiai és Bionikai Kar (PPKE) 11 0.091 false true true Author 10044620 /api/author/10044620 Erdő Franciska (biológia, neurodegenerativ betegségek, gyógyszerkutatás, mikrodialízis, in vivo állatmodellek, innovatív gyógyszerformák, gyógyszerpenetráció) Erdő Franciska DUPLUM true 10044620 true Erdő Franciska true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PublicationIdentifier 28466262 /api/publicationidentifier/28466262 DOI: 10.3390/bioengineering12030230 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.3390/bioengineering12030230 https://doi.org/10.3390/bioengineering12030230 false true PublicationIdentifier 29526325 /api/publicationidentifier/29526325 PPKE Publikáció: 2733 SwordSource 10035 /api/publicationsource/10035 PPKE Publikáció PublicationSourceType 10007 /api/publicationsourcetype/10007 Repozitórium true true true PPKE Publikáció PPCE Publications https://publikacio.ppke.hu/id/eprint/@@@ true true true GREEN true IDENTICAL 2733 https://publikacio.ppke.hu/id/eprint/2733 false true PublicationIdentifier 29223841 /api/publicationidentifier/29223841 WoS: 001454023900001 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001454023900001 https://www.webofscience.com/wos/woscc/full-record/001454023900001 true true PublicationIdentifier 29223824 /api/publicationidentifier/29223824 Scopus: 105001364840 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 105001364840 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105001364840 true true PublicationIdentifier 29223827 /api/publicationidentifier/29223827 PubMed: 40150694 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 40150694 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40150694&dopt=Abstract true true SjrRating 11560761 /api/sjrrating/11560761 sjr:Q2 (2025) Scopus - Bioengineering BIOENGINEERING 2306-5354 61 0.49 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1502 /api/classificationexternal/1502 Scopus - Bioengineering true 1502 true Q2 DIRECT true true Reference 64062170 /api/reference/64062170 1. Moxon 2020: Application of Physiologically Based Kinetic (PBK) Modelling in the next Generation Risk Assessment of Dermally Applied Consumer Products., Toxicol. Vitr., 63, p. 104746, DOI: 10.1016/j.tiv.2019.104746 1 10.1016/j.tiv.2019.104746 false true Reference 64062171 /api/reference/64062171 2. Riabinin, A., Pankratova, M., Rogovaya, O., Vorotelyak, E., Terskikh, V., and Vasiliev, A. (2024). Ideal Living Skin Equivalents, From Old Technologies and Models to Advanced Ones: The Prospects for an Integrated Approach. BioMed Res. Int., 2024., DOI: 10.1155/2024/9947692 2 10.1155/2024/9947692 false true Reference 64062172 /api/reference/64062172 3. Albanna, M., Binder, K.W., Murphy, S.V., Kim, J., Qasem, S.A., Zhao, W., Tan, J., El-Amin, I.B., Dice, D.D., and Marco, J. (2019). In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds. Sci. Rep., 9., DOI: 10.1038/s41598-018-38366-w 3 10.1038/s41598-018-38366-w false true Reference 64062173 /api/reference/64062173 4. Mania, S., Banach-Kopeć, A., Maciejewska, N., Czerwiec, K., Słonimska, P., Deptuła, M., Baczyński-Keller, J., Pikuła, M., Sachadyn, P., and Tylingo, R. (2024). From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour. Molecules, 29., DOI: 10.20944/preprints202408.1701.v1 4 10.20944/preprints202408.1701.v1 false true Reference 64062174 /api/reference/64062174 5. Ramakrishnan 2022: Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl Cellulose-Fibrinogen Based Bioink for 3D Bioprinting of Skin Tissue Constructs., Carbohydr. Polym. Technol. Appl., 3, p. 100184 5 false true Reference 64062175 /api/reference/64062175 6. Loukelis, K., Koutsomarkos, N., Mikos, A.G., and Chatzinikolaidou, M. (2024). Advances in 3D Bioprinting for Regenerative Medicine Applications. Regen. Biomater., 11., DOI: 10.1093/rb/rbae033 6 10.1093/rb/rbae033 false true Reference 64062176 /api/reference/64062176 7. 2019: Matrix Molecules and Skin Biology., Semin. Cell Dev. Biol., 89, p. 136, DOI: 10.1016/j.semcdb.2018.07.025 7 10.1016/j.semcdb.2018.07.025 false true Reference 64062177 /api/reference/64062177 8. Chun, H.J., Reis, R.L., Motta, A., and Khang, G. (2020). Alginate Hydrogels: A Tool for 3D Cell Encapsulation, Tissue Engineering, and Biofabrication. Biomimicked Biomaterials: Advances in Tissue Engineering and Regenerative Medicine, Springer., DOI: 10.1007/978-981-15-3262-7 8 10.1007/978-981-15-3262-7 false true Reference 64062178 /api/reference/64062178 9. Wei, Z., Zuo, Y., Wu, E., Huang, L., Qian, Y., Wang, J., and Chen, Z. (2024). Highly Biocompatible, Antioxidant and Antibacterial Gelatin Methacrylate/Alginate—Tannin Hydrogels for Wound Healing. Int. J. Biol. Macromol., 279., DOI: 10.1016/j.ijbiomac.2024.135417 9 10.1016/j.ijbiomac.2024.135417 false true Reference 64062179 /api/reference/64062179 10. Raucci, M.G., D’Amora, U., Ronca, A., Demitri, C., and Ambrosio, L. (2019). Bioactivation Routes of Gelatin-Based Scaffolds to Enhance at Nanoscale Level Bone Tissue Regeneration. Front. Bioeng. Biotechnol., 7., DOI: 10.3389/fbioe.2019.00027 10 10.3389/fbioe.2019.00027 false true Reference 64062180 /api/reference/64062180 11. Somasekharan 2021: Biofabrication of Skin Tissue Constructs Using Alginate, Gelatin and Diethylaminoethyl Cellulose Bioink., Int. J. Biol. Macromol., 189, p. 398, DOI: 10.1016/j.ijbiomac.2021.08.114 11 10.1016/j.ijbiomac.2021.08.114 false true Reference 64062181 /api/reference/64062181 12. Seok, J.M., Ahn, M., Kim, D., Lee, J.-S., Lee, D., Choi, M.-J., Yeo, S.J., Lee, J.H., Lee, K., and Kim, B.S. (2024). Decellularized Matrix Bioink with Gelatin Methacrylate for Simultaneous Improvements in Printability and Biofunctionality. Int. J. Biol. Macromol., 262., DOI: 10.1016/j.ijbiomac.2024.130194 12 10.1016/j.ijbiomac.2024.130194 false true Reference 64062182 /api/reference/64062182 13. Xu, L., Zhang, Z., Jorgensen, A.M., Yang, Y., Jin, Q., Zhang, G., Cao, G., Fu, Y., Zhao, W., and Ju, J. (2023). Bioprinting a Skin Patch with Dual-Crosslinked Gelatin (GelMA) and Silk Fibroin (SilMA): An Approach to Accelerating Cutaneous Wound Healing. Mater. Today Bio, 18., DOI: 10.1016/j.mtbio.2023.100550 13 10.1016/j.mtbio.2023.100550 false true Reference 64062183 /api/reference/64062183 14. Jin 2021: Three-Dimensional Bioprinting of a Full-Thickness Functional Skin Model Using Acellular Dermal Matrix and Gelatin Methacrylamide Bioink., Acta Biomater., 131, p. 248, DOI: 10.1016/j.actbio.2021.07.012 14 10.1016/j.actbio.2021.07.012 false true Reference 64062184 /api/reference/64062184 15. Piao 2021: Biomedical Applications of Gelatin Methacryloyl Hydrogels., Eng. Regen., 2, p. 47 15 false true Reference 64062185 /api/reference/64062185 16. Gaglio, C.G., Baruffaldi, D., Pirri, C.F., Napione, L., and Frascella, F. (2024). GelMA Synthesis and Sources Comparison for 3D Multimaterial Bioprinting. Front. Bioeng. Biotechnol., 12., DOI: 10.3389/fbioe.2024.1383010 16 10.3389/fbioe.2024.1383010 false true Reference 64062186 /api/reference/64062186 17. Turksen, K. (2014). Growth and Differentiation of HaCaT Keratinocytes. Epidermal Cells: Methods and Protocols, Springer., DOI: 10.1007/978-1-4939-1224-7 17 10.1007/978-1-4939-1224-7 false true Reference 64062187 /api/reference/64062187 18. Temirel, M., Dabbagh, S.R., and Tasoglu, S. (2022). Shape Fidelity Evaluation of Alginate-Based Hydrogels through Extrusion-Based Bioprinting. J. Funct. Biomater., 13., DOI: 10.3390/jfb13040225 18 10.3390/jfb13040225 false true Reference 64062188 /api/reference/64062188 19. Zhang, J., Wehrle, E., Vetsch, J.R., Paul, G.R., Rubert, M., and Müller, R. (2019). Alginate Dependent Changes of Physical Properties in 3D Bioprinted Cell-Laden Porous Scaffolds Affect Cell Viability and Cell Morphology. Biomed. Mater., 14., DOI: 10.1088/1748-605X/ab3c74 19 10.1088/1748-605X/ab3c74 false true Reference 64062189 /api/reference/64062189 20. Svanström, A., Rosendahl, J., Salerno, S., Leiva, M.C., Gregersson, P., Berglin, M., Bogestål, Y., Lausmaa, J., Oko, A., and Chinga-Carrasco, G. (2021). Optimized Alginate-Based 3D Printed Scaffolds as a Model of Patient Derived Breast Cancer Microenvironments in Drug Discovery. Biomed. Mater., 16., DOI: 10.1088/1748-605X/ac0451 20 10.1088/1748-605X/ac0451 false true Reference 64062190 /api/reference/64062190 21. (2025, February 05). GelMA C, CELLINK. Available online: https://www.cellink.com/product/gelma-c/. 21 false true Reference 64062191 /api/reference/64062191 22. de Castilho, A.R.F., Rosalen, P.L., Oliveira, M.Y., Burga-Sánchez, J., Duarte, S., Murata, R.M., and Rontani, R.M.P. (2024). Bioactive Compounds Enhance the Biocompatibility and the Physical Properties of a Glass Ionomer Cement. J. Funct. Biomater., 15., DOI: 10.3390/jfb15110332 22 10.3390/jfb15110332 false true Reference 64062192 /api/reference/64062192 23. de Castilho, A.R.F., Rosalen, P., Oliveira, M., Sánchez, J., Duarte, S., and Murata, R. (2019, January 18). Cytotoxicity and Physical Properties of Glass Ionomer Cement Containing Flavonoids IADR Abstract Archives. Proceedings of the 2019 IADR/AADR/CADR General Session, Vancouver, BC, Canada. 23 false true Reference 64062193 /api/reference/64062193 24. Orellana, E.A., and Kasinski, A.L. (2016). Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc., 6., DOI: 10.21769/BioProtoc.1984 24 10.21769/BioProtoc.1984 false true Reference 64062194 /api/reference/64062194 25. Kocsis, D., Dhinakaran, S., Pandey, D., Laki, A.J., Laki, M., Sztankovics, D., Lengyel, M., Vrábel, J., Naszlady, M.B., and Sebestyén, A. (2024). Fluid Dynamics Optimization of Microfluidic Diffusion Systems for Assessment of Transdermal Drug Delivery: An Experimental and Simulation Study. Sci. Pharm., 92., DOI: 10.3390/scipharm92020035 25 10.3390/scipharm92020035 false true Reference 64062195 /api/reference/64062195 26. Lukács, B., Bajza, Á., Kocsis, D., Csorba, A., Antal, I., Iván, K., Laki, A.J., and Erdő, F. (2019). Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing. Pharmaceutics, 11., DOI: 10.3390/pharmaceutics11090445 26 10.3390/pharmaceutics11090445 false true Reference 64062196 /api/reference/64062196 27. Szederkényi, G., Kocsis, D., Vághy, M.A., Czárán, D., Sasvári, P., Lengyel, M., Naszlady, M.B., Kreis, F., Antal, I., and Csépányi-Kömi, R. (2024). Mathematical Modeling of Transdermal Delivery of Topical Drug Formulations in a Dynamic Microfluidic Diffusion Chamber in Health and Disease. PLoS ONE, 19., DOI: 10.1371/journal.pone.0299501 27 10.1371/journal.pone.0299501 false true Reference 64062197 /api/reference/64062197 28. Bajza, Á., Kocsis, D., Berezvai, O., Laki, A.J., Lukács, B., Imre, T., Iván, K., Szabó, P., and Erdő, F. (2020). Verification of P-Glycoprotein Function at the Dermal Barrier in Diffusion Cells and Dynamic “Skin-On-A-Chip” Microfluidic Device. Pharmaceutics, 12., DOI: 10.3390/pharmaceutics12090804 28 10.3390/pharmaceutics12090804 false true Reference 64062198 /api/reference/64062198 29. Varga-Medveczky, Z., Kocsis, D., Naszlady, M.B., Fónagy, K., and Erdő, F. (2021). Skin-on-a-Chip Technology for Testing Transdermal Drug Delivery-Starting Points and Recent Developments. Pharmaceutics, 13., DOI: 10.3390/pharmaceutics13111852 29 10.3390/pharmaceutics13111852 false true Reference 64062199 /api/reference/64062199 30. Franco 2024: Misleading Pore Size Measurements in Gelatin and Alginate Hydrogels Revealed by Confocal Microscopy., Tissue Eng. Part C Methods, 30, p. 307, DOI: 10.1089/ten.tec.2024.0117 30 10.1089/ten.tec.2024.0117 false true Reference 64062200 /api/reference/64062200 31. Zhuang, P., Ng, W.L., An, J., Chua, C.K., and Tan, L.P. (2019). Layer-by-Layer Ultraviolet Assisted Extrusion-Based (UAE) Bioprinting of Hydrogel Constructs with High Aspect Ratio for Soft Tissue Engineering Applications. PLoS ONE, 14., DOI: 10.1371/journal.pone.0216776 31 10.1371/journal.pone.0216776 false true Reference 64062201 /api/reference/64062201 32. Hafezi, M., Khorasani, S.N., Khalili, S., and Neisiany, R.E. (2023). Self-Healing Interpenetrating Network Hydrogel Based on GelMA/Alginate/Nano-Clay. Int. J. Biol. Macromol., 242., DOI: 10.1016/j.ijbiomac.2023.124962 32 10.1016/j.ijbiomac.2023.124962 false true Reference 64062202 /api/reference/64062202 33. Karam, M., Faraj, M., Jaffa, M.A., Jelwan, J., Aldeen, K.S., Hassan, N., Mhanna, R., and Jaffa, A.A. (2024). Development of Alginate and Alginate Sulfate/Polycaprolactone Nanoparticles for Growth Factor Delivery in Wound Healing Therapy. Biomed. Pharmacother., 175., DOI: 10.1016/j.biopha.2024.116750 33 10.1016/j.biopha.2024.116750 false true Reference 64062203 /api/reference/64062203 34. Trapani, A., Corbo, F., Stefàno, E., Capobianco, L., Muscella, A., Marsigliante, S., Cricenti, A., Luce, M., Becerril, D., and Bellucci, S. (2022). Oxidized Alginate Dopamine Conjugate: A Study to Gain Insight into Cell/Particle Interactions. J. Funct. Biomater., 13., DOI: 10.3390/jfb13040201 34 10.3390/jfb13040201 false true Reference 64062204 /api/reference/64062204 35. Dragomir, D.C., Carbunaru, V., Moldovan, C.A., Lascar, I., Dontu, O., Ristoiu, V., Gheorghe, R., Oproiu, A.M., Firtat, B., and Franti, E. (2023). Biocompatibility Analysis of GelMa Hydrogel and Silastic RTV 9161 Elastomer for Encapsulation of Electronic Devices for Subdermal Implantable Devices. Coatings, 13., DOI: 10.3390/coatings13010019 35 10.3390/coatings13010019 false true Reference 64062205 /api/reference/64062205 36. Bakshi 2023: Biocompatible Alginate Film Crosslinked with Ca2+ and Zn2+ Possesses Antibacterial, Antiviral, and Anticancer Activities., ACS Omega, 8, p. 24396, DOI: 10.1021/acsomega.3c01935 36 10.1021/acsomega.3c01935 false true Reference 64062206 /api/reference/64062206 37. Cameron 1990: Caffeine and Human Cerebral Blood Flow: A Positron Emission Tomography Study., Life Sci., 47, p. 1141, DOI: 10.1016/0024-3205(90)90174-P 37 10.1016/0024-3205(90)90174-P false true Reference 64062207 /api/reference/64062207 38. Koo 2007: Protection from Photodamage by Topical Application of Caffeine after Ultraviolet Irradiation., Br. J. Dermatol., 156, p. 957, DOI: 10.1111/j.1365-2133.2007.07812.x 38 10.1111/j.1365-2133.2007.07812.x false true Reference 64062208 /api/reference/64062208 39. Leonardi 2008: The Effect of Topical Caffeine on the Morphology of Swine Hypodermis as Measured by Ultrasound., J. Cosmet. Dermatol., 7, p. 232, DOI: 10.1111/j.1473-2165.2008.00397.x 39 10.1111/j.1473-2165.2008.00397.x false true Reference 64062209 /api/reference/64062209 40. Todo 2021: Improvement of Skin Permeation of Caffeine, a Hydrophilic Drug, by the Application of Water Droplets Provided by a Novel Humidifier Device., Chem. Pharm. Bull., 69, p. 727, DOI: 10.1248/cpb.c21-00110 40 10.1248/cpb.c21-00110 false true Reference 64062210 /api/reference/64062210 41. Kurzyk, A., Szumera-Ciećkiewicz, A., Miłoszewska, J., and Chechlińska, M. (2024). 3D Modeling of Normal Skin and Cutaneous Squamous Cell Carcinoma. A Comparative Study in 2D Cultures, Spheroids, and 3D Bioprinted Systems. Biofabrication, 16., DOI: 10.1088/1758-5090/ad2b06 41 10.1088/1758-5090/ad2b06 false true Reference 64062211 /api/reference/64062211 42. Inci 2018: Bioinks for 3D Bioprinting: An Overview., Biomater. Sci., 6, p. 915, DOI: 10.1039/C7BM00765E 42 10.1039/C7BM00765E false true Reference 64062212 /api/reference/64062212 43. Rutz 2017: Toward Next-Generation Bioinks: Tuning Material Properties Pre- and Post-Printing to Optimize Cell Viability., MRS Bull., 42, p. 563, DOI: 10.1557/mrs.2017.162 43 10.1557/mrs.2017.162 false true Reference 64062213 /api/reference/64062213 44. Hölzl, K., Lin, S., Tytgat, L., Van Vlierberghe, S., Gu, L., and Ovsianikov, A. (2016). Bioink Properties before, during and after 3D Bioprinting. Biofabrication, 8., DOI: 10.1088/1758-5090/8/3/032002 44 10.1088/1758-5090/8/3/032002 false true Reference 64062214 /api/reference/64062214 45. Schulik, J., Salehi, S., Boccaccini, A.R., Schrüfer, S., Schubert, D.W., Arkudas, A., Kengelbach-Weigand, A., Horch, R.E., and Schmid, R. (2023). Comparison of the Behavior of 3D-Printed Endothelial Cells in Different Bioinks. Bioengineering, 10., DOI: 10.3390/bioengineering10070751 45 10.3390/bioengineering10070751 false true Reference 64062215 /api/reference/64062215 46. Chopin-Doroteo, M., Mandujano-Tinoco, E.A., and Krötzsch, E. (2021). Tailoring of the Rheological Properties of Bioinks to Improve Bioprinting and Bioassembly for Tissue Replacement. Biochim. Biophys. Acta Gen. Subj., 1865., DOI: 10.1016/j.bbagen.2020.129782 46 10.1016/j.bbagen.2020.129782 false true Reference 64062216 /api/reference/64062216 47. Schwab 2020: Printability and Shape Fidelity of Bioinks in 3D Bioprinting., Chem. Rev., 120, p. 11028, DOI: 10.1021/acs.chemrev.0c00084 47 10.1021/acs.chemrev.0c00084 false true Reference 64062217 /api/reference/64062217 48. Chimene 2020: Hydrogel Bioink Reinforcement for Additive Manufacturing: A Focused Review of Emerging Strategies., Adv. Mater., 32, p. 1902026, DOI: 10.1002/adma.201902026 48 10.1002/adma.201902026 false true Reference 64062218 /api/reference/64062218 49. Williams 2018: A Perspective on the Physical, Mechanical and Biological Specifications of Bioinks and the Development of Functional Tissues in 3D Bioprinting., Bioprinting, 9, p. 19, DOI: 10.1016/j.bprint.2018.02.003 49 10.1016/j.bprint.2018.02.003 false true Reference 64062219 /api/reference/64062219 50. Ramasamy, S., Davoodi, P., Vijayavenkataraman, S., Teoh, J.H., Thamizhchelvan, A.M., Robinson, K.S., Wu, B., Fuh, J.Y.H., DiColandrea, T., and Zhao, H. (2021). Optimized Construction of a Full Thickness Human Skin Equivalent Using 3D Bioprinting and a PCL/Collagen Dermal Scaffold. Bioprinting, 21., DOI: 10.1016/j.bprint.2020.e00123 50 10.1016/j.bprint.2020.e00123 false true /api/publication/35784145 Kocsis Dorottya et al. In Vitro Functional and Structural Evaluation of Low-Complexity Artificial Human Epidermis for 3D Tissue Engineering. (2025) BIOENGINEERING 2306-5354 12 3<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10080212"><a href="/gui2/?type=authors&mode=browse&sel=10080212" target="_blank">Kocsis Dorottya (<span class="authorship-author-name">Kocsis Dorottya</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10074525"><a href="/gui2/?type=authors&mode=browse&sel=10074525" target="_blank">Sztankovics Dániel (<span class="authorship-author-name">Sztankovics Dániel</span> <span class="authorAux-mtmt"> Onkológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Patológiai és Kísérleti Rákkutató Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10069255"><a href="/gui2/?type=authors&mode=browse&sel=10069255" target="_blank">Józsa Liza (<span class="authorship-author-name">Józsa Liza</span> <span class="authorAux-mtmt"> gyógyszertechnológia</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszertechnológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10080217"><a href="/gui2/?type=authors&mode=browse&sel=10080217" target="_blank">Németh Afrodité (<span class="authorship-author-name">Németh Afrodité</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10030126"><a href="/gui2/?type=authors&mode=browse&sel=10030126" target="_blank">Garay Tamás ✉ (<span class="authorship-author-name">Garay Tamás</span> <span class="authorAux-mtmt"> sejtbiológia, tumorbiológia</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/Információs Technológiai és Bionikai Kar; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Klinikum">K</span>/Belgyógyászati és Onkológiai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10084103"><a href="/gui2/?type=authors&mode=browse&sel=10084103" target="_blank">Naszlady Márton Bese ✉ (<span class="authorship-author-name">Naszlady Márton Bese</span> <span class="authorAux-mtmt"> Műszaki tudományok</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/Információs Technológiai és Bionikai Kar</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020628"><a href="/gui2/?type=authors&mode=browse&sel=10020628" target="_blank">Lengyel Miléna (<span class="authorship-author-name">Lengyel Miléna</span> <span class="authorAux-mtmt"> Gyógyszertechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészeti Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10001742"><a href="/gui2/?type=authors&mode=browse&sel=10001742" target="_blank">Vecsernyés Miklós (<span class="authorship-author-name">Vecsernyés Miklós</span> <span class="authorAux-mtmt"> gyógyszerésztudomány</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszertechnológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020626"><a href="/gui2/?type=authors&mode=browse&sel=10020626" target="_blank">Antal István (<span class="authorship-author-name">Antal István</span> <span class="authorAux-mtmt"> Gyógyszertechnológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészeti Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000745"><a href="/gui2/?type=authors&mode=browse&sel=10000745" target="_blank">Sebestyén Anna (<span class="authorship-author-name">Sebestyén Anna</span> <span class="authorAux-mtmt"> Tumorbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Patológiai és Kísérleti Rákkutató Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10044620"><a href="/gui2/?type=authors&mode=browse&sel=10044620" target="_blank">Erdő Franciska ✉ (<span class="authorship-author-name">Erdő Franciska</span> <span class="authorAux-mtmt"> biológia, neurodegenerativ betegségek, gyógysze...</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Katolikus Egyetem">PPKE</span>/Információs Technológiai és Bionikai Kar</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35784145" target="_blank">In Vitro Functional and Structural Evaluation of Low-Complexity Artificial Human Epidermis for 3D Tissue Engineering</a></div> <div> <span class="journal-title">BIOENGINEERING</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2306-5354">2306-5354</a>)</span>: <span class="journal-volume">12</span> <span class="journal-issue">3</span> <span class="page"> Paper 230. 16 p. </span> <span class="year">(2025)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.3390/bioengineering12030230" target="_blank" href="https://doi.org/10.3390/bioengineering12030230"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="2733" target="_blank" href="https://publikacio.ppke.hu/id/eprint/2733"> PPKE Publikáció </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001454023900001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001454023900001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="105001364840" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-105001364840"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="40150694" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=40150694&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Bioengineering&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35784145&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 5 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35784145</span> | <span class="status-data status-APPROVED"> Nyilvános </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> Thematic Excellence Program(TKP2021-EGA-42) </div> <div class="lastModified">Utolsó módosítás: 2026.09.17. 14:25 Áncsán Gizella (SE admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: Ministry of Innovation and Technology, Hungary [TKP2021-EGA-42, 2024-2.1.1-EKOP]; National Research Development and Innovation Fund [TKP2021]; Pazmany Peter Catholic University Research Scholarship Program (Egyetemi Kutatoi Osztondij Program) Funding text: This work was supported by TKP2021-EGA-42 and 2024-2.1.1-EKOP grants, funded by the Ministry of...</pre> </div></div>