Víztudományi és Vízbiztonsági Nemzeti Laboratórium(RRF-2.3.1-21-2022-00008) Támogató:
NKFIH
The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout
Europe, primarily inhabiting stony upper sections of stream networks. This study presents
an updated genome assembly of B. barbatula , contributing to the species’ available
genomic resources for downstream applications such as conservation genetics. The draft
assembly was 550 Mbp in size, with an N50 of 11.21 Mbp. We used the species’ available
chromosome scaffolds to finish the genome. The final assembly had a BUSCO score of
96.7%. We identified 23270 protein-coding genes, and the proteome exhibited high completeness
with BUSCO (93.1%) and OMArk (90.81%). Despite using multiple approaches to reduce
duplicate contigs, we observed a relatively high duplicate ratio of 6.1% (BUSCO) and
8.52% (OMArk) in the annotations. We aimed to find microsatellite loci present in
both the species’ publicly available genome and the new assembly to aid marker development
for downstream analyses. This dataset serves as a reference for genomic analysis and
is useful for developing markers to study the species’ biodiversity and support conservation
efforts.
