This work demonstrates that the recently introduced TADA-MS method (originally designed
in a CE instrument)
can be applied in a simple, low-pressure flow injection system, which is more widely
accessible in an average MS
analytical laboratory. An essential part of the system is an internal sample chamber
micro-scale injector. A
sample volume of 0.5 μL was mobilized by a syringe pump. The analytical applicability
of the proposed system
was tested by analyzing concanavalin A protein under denaturing and native conditions.
The obtained results
demonstrated that in the proposed low-pressure flow injection TADA-MS system the capillaries
of both 50 μm
and 100 μm i.d., and both the conventional ESI interface and the coaxial sheath-liquid
CE-ESI interface can be
efficiently applied – the obtained MS spectra were similar.
