Antibody-drug conjugates (ADCs) represent a therapeutic modality that guides chemotherapies
to tumoral cells by using antibodies against tumor-associated antigens (TAAs). The
antibody and the chemotherapy or payload are attached by a chemical structure called
the linker. The strategy for the development of this type of drug was based on several
rational pillars, including the use of a very potent payload and the use of specific
antibodies acting only on antigens expressed on tumoral cells. In this article, by
using data from all approved ADCs that have received regulatory approval, we analyze
the potential contribution of each ADC component to preclinical activity. We suggest
that payload potency and the drug-to-antibody ratio (DAR) have a less relevant role
in relation to efficacy than previously considered. Additionally, we have observed
that some ADCs have been developed against antigens also present in non-transformed
tissues, which could suggest that TAA specificity is not a mandatory requirement.
Finally, we have identified that ADCs with payloads harboring more favorable physicochemical
characteristics showed better potential activity. In this article, we also review
other aspects that should be taken into consideration for ADC design, including linker
structure, stability, conjugation type, pharmacokinetics, receptor internalization,
and recycling. Based on currently available data, our study summaries different concepts
that should be considered in the design of novel ADCs in the future.
