European Union’s Horizon 2020 research and innovation program(952491-AmReSu)
(BO/00286/22/5)
Szakterületek:
Orvosi mikrobiológia
Multidrug-resistant Acinetobacter baumannii is a major concern in healthcare institutions
worldwide. Several reports described the dissemination of A. baumannii high-risk clones
that are responsible for a high number of difficult-to-treat infections. In our study,
19 multidrug-resistant A. baumannii strains from Budapest, Hungary, were investigated
based on whole-genome sequencing (WGS). The obtained results were analysed together
with data from 433 strains of A. baumannii from the Pathogenwatch database. WGS analysis
of 19 A. baumannii strains detected that 12 belonged to ST2 and seven belonged to
ST636. Among ST2 strains, 11 out of 12 carried either blaOXA-23 or blaOXA-58 genes;
however, all strains of ST636 uniformly carried blaOXA-72 gene. All strains of ST2
and ST636 carried blaOXA-66 and blaADC-25 genes. Based on core genome multilocus sequence
typing (cgMLST), 10 strains of ST2 belonged to cgMLST906, one strain to cgMLST458,
and one strain to cgMLST1320; by contrast, all strains of ST636 belonged to cgMLST1178.
Certain virulence determinants were present in all strains of both ST2 and ST636,
namely, Ata, Bap, BfmRS, T2SS and PNAG. Interestingly, OmpA was present in all strains
of ST2, but it was absent in all strains of ST636. Comparative analysis of 19 strains
of this study and the collection of 433 isolates from Pathogenwatch database, proved
a diverse clonal distribution of high-risk A. baumannii clones in Europe. The major
clone in Europe is ST2, which is present all over the continent. However, ST636 has
been mainly reported in Eastern Europe. Interestingly, cgMLSTs of ST2 correspond to
the production of different beta-lactamases, namely, OXA-82 in cgMLST116, OXA-72 in
cgMLST506, and cgMLST556, PER-1 in cgMLST456 and cgMLST1041. Our study demonstrates
that the ST2 high-risk clone of A. baumannii is the most widespread in Europe; however,
based on cgMLST analysis, a detailed detection of beta-lactamase production can be
determined.
