https://m2.mtmt.hu/ hun 2026-09-22 02:55 JournalArticle 35594374 VALIDATED true Export Date: 26 November 2024 Correspondence Address: Vág, J.; Department of Restorative Dentistry and Endodontics, H-1088 Budapest, Szentkirályi utca 47, Hungary; email: vag.janos@semmelweis.hu 0 false 2026-05-24T11:46:17.116+0000 2025-10-19T16:18:30.279+0000 2024-11-21T19:53:38.053+0000 true 10018804 Szalóky-Siki Ágnes /api/admin/10018804 Admin true 10018804 2026-04-21T15:26:17.759+0000 2024-11-25T09:52:14.435+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Nagy, Tamás László Dose-related effect of acetylcholine on human gingival blood flow. true true /api/journal/10016258 REVIEWED true false 10016258 false 10016258 true 1472-6831 1472-6831 Journal FOREIGN 24 1 1398 1398 10 2024 This study investigates the dose-response relationship of acetylcholine (ACh) on healthy human gingival blood flow (GBF). Understanding this dose-response relationship contributes to studying vasodilatory mechanisms in various pathological conditions.The study involved 22 young healthy men (21 - 32 years) to investigate the dose-response relationship of ACh on GBF. Semi-circular wells were created on the labial surface of the upper right second incisor (FDI #12) and upper left first incisor (FDI #21), including the gingival sulcus, for the application of drugs. ACh-chloride solutions at 0.1, 1, and 10 mg/mL were administered to the gingival sulcus of tooth FDI #12 with a Hamilton syringe. Physiological saline was applied on the contralateral side to FDI #21 as a control. The GBF was measured non-invasively by the laser speckle contrast imaging method in four 1mm high adjacent regions: coronal, midway1, midway2, and apical, and was expressed in a laser speckle perfusion unit (LSPU). After the baseline blood flow recording, ACh doses were applied sequentially, with washout periods in between. Data were statistically analyzed using a linear mixed model.The GBF did not change on the saline site throughout the experiment. The GBF was significantly higher at the coronal region after all ACh doses (baseline: 218±31 LSPU, and 227±38 LSPU p < 0.05, 239±40 LSPU p < 0.001, 291±54 LSPU p < 0.001, respectively) compared to the saline. It was also elevated following 1 and 10 mg/mL at the midway1 (245±48 LSPU, p < 0.05, 293±65 LSPU p < 0.001). At midway2 and apical, only the 10 mg/mL dose was effective (285±71 LSPU, p < 0.001; 302±82 LSPU, p < 0.001).Our findings suggest a dose-dependent vasodilation to ACh, emphasizing its role in human gingival microcirculation. Only the 10 mg/mL ACh could evoke remote vasodilation 3 mm from the application. The described method could facilitate the investigation of endothelium-dependent vasodilation in disorders affecting microcirculation, such as periodontitis or diabetes. 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src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10084900"><a href="/gui2/?type=authors&mode=browse&sel=10084900" target="_blank">Nagy Tamás László (<span class="authorship-author-name">Nagy Tamás László</span> <span class="authorAux-mtmt"> Endodoncia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Fogorvostudományi Kar">FOK</span>/Helyreállító Fogászati és Endodonciai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10051827"><a href="/gui2/?type=authors&mode=browse&sel=10051827" target="_blank">Mikecs Barbara (<span class="authorship-author-name">Mikecs Barbara</span> <span class="authorAux-mtmt"> Konzerváló fogászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Fogorvostudományi Kar">FOK</span>/Helyreállító Fogászati és Endodonciai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10018912"><a href="/gui2/?type=authors&mode=browse&sel=10018912" target="_blank">Lohinai Zsolt M (<span class="authorship-author-name">Lohinai Zsolt</span> <span class="authorAux-mtmt"> Konzerváló fogászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Fogorvostudományi Kar">FOK</span>/Helyreállító Fogászati és Endodonciai Klinika</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10035110"><a href="/gui2/?type=authors&mode=browse&sel=10035110" target="_blank">Vág János ✉ (<span class="authorship-author-name">Vág János</span> <span class="authorAux-mtmt"> Fogászat</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Fogorvostudományi Kar">FOK</span>/Helyreállító Fogászati és Endodonciai Klinika</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35594374" target="_blank">Dose-related effect of acetylcholine on human gingival blood flow.</a></div> <div> <span class="journal-title">BMC ORAL HEALTH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1472-6831">1472-6831</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1472-6831">1472-6831</a>)</span>: <span class="journal-volume">24</span> <span class="journal-issue">1</span> <span class="page"> Paper 1398. 10 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1186/s12903-024-05169-7" target="_blank" href="https://doi.org/10.1186/s12903-024-05169-7"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" 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</span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Dentistry (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 2</span> | Független: 2 | Függő: 0 | Nem jelölt: 0 | WoS jelölt: 2 | WoS/Scopus jelölt:&nbsp;2 | DOI jelölt:&nbsp;2 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35594374&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 6 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35594374</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (EFOP-3.6.2–16-2017–00006), (Open access funding provided by Semmelweis University) </div> <div class="lastModified">Utolsó módosítás: 2025.10.19. 18:18 Szalóky-Siki Ágnes (SE_FOK_Admin5_SIKIÁ, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Export Date: 26 November 2024 Correspondence Address: Vág, J.; Department of Restorative Dentistry and Endodontics, H-1088 Budapest, Szentkirályi utca 47, Hungary; email: vag.janos@semmelweis.hu</pre> </div></div>