https://m2.mtmt.hu/ hun 2026-05-25 23:55 JournalArticle 35578246 VALIDATED true Export Date: 22 April 2026; Cited By: 3; Correspondence Address: G. Tóth; Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hőgyes E. u. 9, 1092, Hungary; email: toth.gergo@semmelweis.hu; 0 false 2026-05-24T11:34:49.804+0000 2026-04-22T09:25:30.307+0000 2024-11-18T16:28:34.108+0000 true 10079694 Lázárné Zalán Zita /api/admin/10079694 Admin Lázárné Zalán Zita (SZTE admin5 GYTK Gyógyszerkémiai Intézet) true 2026-04-22T09:25:30.251+0000 2025-09-09T09:34:03.854+0000 true 10015544 Csajbók Edit /api/admin/10015544 Admin Csajbók Edit (SE_KK_Admin5_CSE, admin) true 10015544 true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Ferencz, E. true true /api/journal/10019966 REVIEWED SCIENTIFIC REPORTS 2045-2322 true false 10019966 false 10019966 true 2045-2322 Journal FOREIGN 14 1 26757 26757 12 2024 In this study, a direct HPLC method was developed to determine the enantiomeric purity of the immunomodulatory drug, ozanimod. A systematic method development process was followed, incorporating risk assessment, identification of critical analytical procedure parameters, initial screening of stationary phases, and software-assisted optimization of method parameters. Eight different polysaccharide-based chiral columns were selected to assess chiral separation of enantiomers under polar organic elution mode. The most promising results were obtained using a methanol:2-propanol mixture on the amylose-based Chiralpak AD column. Following this, systematic modeling was conducted using DryLab software to optimize method conditions, including isocratic eluent composition, temperature, and flow rate. Baseline separation was achieved within fifteen minutes using the optimized parameters: Chiralpak AD column thermostated at 10 °C, and a mobile phase of methanol:2-propanol: diethylamine, 70:30:0.1 (v/v/v %), delivered at a flow rate of 0.8 mL/min. The developed method was validated according to current guidelines and in silico robustness testing was conducted to determine tolerance limits for critical separation parameters and their impact on enantioresolution. Our findings demonstrate the utility of DryLab, typically employed for reversed-phase achiral separations, in optimizing chiral methods even in polar organic mode. Limitations of the selected approach the development of chiral separation methods are also highlighted. © The Author(s) 2024. Funding 2046665 /api/funding/2046665 (FK 146930) false true Funding 2046666 /api/funding/2046666 Nemzeti Kutatási, Fejlesztési és Innovációs Alap(ÚNKP-23-3-II) false true Funding 2046667 /api/funding/2046667 (ÚNKP-23-2-III) false true Funding 2046668 /api/funding/2046668 (EKÖP-2024-57) false true Funding 2046669 /api/funding/2046669 (János Bolyai Research Scholarship) false true true true 2024 true true true false true false GOLD 2026-05-24 false http://www.nature.com/srep/index.html 3 0 3 2 0 3 3 3 3 3 3 2 2 2 1 0 3 2 3 2 0 11 11 Folyóiratcikk Journal Article 24 3 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 2 1 2 1 2026 0 1 1 1 1 Q1 A nemzetközi false false false false 2024-11-17T16:29:12.708+0000 Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); Gyógyszerészi Kémiai Intézet (SE / GYTK) 4 12 2 9 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 121650022 /api/authorship/121650022 Ferencz, E. 1 0.11111111 true false false Ferencz E. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650023 /api/authorship/121650023 Szabó, Z.-I.* 2 0.111 true false false Szabó Z.-I. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650024 /api/authorship/121650024 Zöldhegyi, A. 3 0.11111111 false false false Zöldhegyi A. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650025 /api/authorship/121650025 Dombi, G. [Dombi, Gergely (Gyógyszeranalitik...), szerző] Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); Gyógyszerészi Kémiai Intézet (SE / GYTK) 4 0.11111111 false false false Author 10087450 /api/author/10087450 Dombi Gergely (Gyógyszeranalitika, HPLC) Dombi Gergely true true Dombi G. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650026 /api/authorship/121650026 Molnár, G. [Molnár, Gergely (Királis nagyhaték...), szerző] Gyógyszerészi Kémiai Intézet (SE / GYTK); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE) 5 0.111 false false false Author 10100801 /api/author/10100801 Molnár Gergely (Királis nagyhatékonyságú folyadékkromatográfia) Molnár Gergely true true Molnár G. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650027 /api/authorship/121650027 Dobó, M. [Dobó, Máté (elválasztástechni...), szerző] Gyógyszerészi Kémiai Intézet (SE / GYTK); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE) 6 0.111 false false false Author 10081854 /api/author/10081854 Dobó Máté (elválasztástechnika, (királis HPLC)) Dobó Máté true true Dobó M. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650028 /api/authorship/121650028 Varga, E. 7 0.11111111 false false false Varga E. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650029 /api/authorship/121650029 Molnár, I. 8 0.11111111 false false false Molnár I. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 121650030 /api/authorship/121650030 Tóth, G. ✉ [Tóth, Gergő (gyógyszerkémia/an...), szerző] Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); Gyógyszerészi Kémiai Intézet (SE / GYTK) 9 0.111 false true true Author 10028950 /api/author/10028950 Tóth Gergő (gyógyszerkémia/analitika) Tóth Gergő true 10028950 true Tóth G. true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PublicationIdentifier 27940749 /api/publicationidentifier/27940749 DOI: 10.1038/s41598-024-78415-1 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.1038/s41598-024-78415-1 https://doi.org/10.1038/s41598-024-78415-1 false true PublicationIdentifier 27940750 /api/publicationidentifier/27940750 WoS: 001348897100032 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 001348897100032 https://www.webofscience.com/wos/woscc/full-record/001348897100032 false true PublicationIdentifier 27940748 /api/publicationidentifier/27940748 Scopus: 85208602910 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 85208602910 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85208602910 false true PublicationIdentifier 27944328 /api/publicationidentifier/27944328 PubMed: 39500751 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true IDENTICAL 39500751 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39500751&dopt=Abstract true true PublicationIdentifier 31712053 /api/publicationidentifier/31712053 Egyéb URL: https://www.nature.com/articles/s41598-024-78415-1 PlainSource 40 /api/publicationsource/40 Egyéb URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true IDENTICAL https://www.nature.com/articles/s41598-024-78415-1 https://www.nature.com/articles/s41598-024-78415-1 false true PublicationIdentifier 31876485 /api/publicationidentifier/31876485 Egyéb URL: https://www.scopus.com/pages/publications/85208602910?origin=resultslist PlainSource 40 /api/publicationsource/40 Egyéb URL PublicationSourceType 10006 /api/publicationsourcetype/10006 Link true true true Egyéb URL Other URL @@@ true true 40 true NONE IDENTICAL https://www.scopus.com/pages/publications/85208602910?origin=resultslist https://www.scopus.com/pages/publications/85208602910?origin=resultslist false true Classification 12197 /api/classification/12197 Gyógyszerészet, farmakogenomika, gyógyszerkutatás és tervezés, gyógyszeres kezelés true true Keyword 136 /api/keyword/136 CHIRAL SEPARATION true 136 true Keyword 959 /api/keyword/959 METHANOL true 959 true Keyword 959 /api/keyword/959 METHANOL true 959 true Keyword 3177 /api/keyword/3177 2-PROPANOL true 3177 true Keyword 3338 /api/keyword/3338 Chemistry true 3338 true Keyword 3493 /api/keyword/3493 Software true 3493 true Keyword 3493 /api/keyword/3493 Software true 3493 true Keyword 3735 /api/keyword/3735 AMYLOSE true 3735 true Keyword 3735 /api/keyword/3735 AMYLOSE true 3735 true Keyword 4383 /api/keyword/4383 Chromatography, High Pressure Liquid true 4383 true Keyword 4386 /api/keyword/4386 high performance liquid chromatography true 4386 true Keyword 1007497 /api/keyword/1007497 Stereoisomerism true 1007497 true Keyword 1007497 /api/keyword/1007497 Stereoisomerism true 1007497 true Keyword 1011332 /api/keyword/1011332 Polysaccharides true 1011332 true Keyword 1011333 /api/keyword/1011333 polysaccharide true 1011333 true Keyword 1011336 /api/keyword/1011336 carbamic acid derivative true 1011336 true Keyword 1018664 /api/keyword/1018664 2 propanol true 1018664 true Keyword 1328022 /api/keyword/1328022 procedures true 1328022 true Keyword 1352052 /api/keyword/1352052 Drylab true 1352052 true Keyword 1498938 /api/keyword/1498938 ozanimod true true Keyword 1912934 /api/keyword/1912934 AQbD true true Keyword 3656244 /api/keyword/3656244 Computer-assisted method development true true Keyword 3656245 /api/keyword/3656245 Polysaccharide-type chiral column true true Keyword 3656246 /api/keyword/3656246 Chiralpak AD true true Keyword 3656247 /api/keyword/3656247 Phenylcarbamates true true MtaRating 519359 /api/mtarating/519359 IX. Szociológiai Tudományos Bizottság:A nemzetközi SCIENTIFIC REPORTS 2045-2322 RatingType 10007 /api/ratingtype/10007 IX. Szociológiai Tudományos Bizottság SZTB Institute 14102 /api/institute/14102 Szociológiai Tudományos Bizottság IXGJO SZTB [1901-] 0 false true false Szociológiai Tudományos Bizottság Committee on Sociology true 14102 true true true A nemzetközi true 19359 true MtaRating 11551161 /api/mtarating/11551161 IX. Regionális Tudományok Bizottsága:B nemzetközi SCIENTIFIC REPORTS 2045-2322 RatingType 10010 /api/ratingtype/10010 IX. Regionális Tudományok Bizottsága RegTB Institute 14100 /api/institute/14100 Regionális Tudományok Bizottsága IXGJO RTB [1901-] 0 false true false Regionális Tudományok Bizottsága Committee on Regional Studies true 14100 true true true B nemzetközi false true SjrRating 11736109 /api/sjrrating/11736109 sjr:Q1 (2024) Scopus - Multidisciplinary SCIENTIFIC REPORTS 2045-2322 23 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 1000 /api/classificationexternal/1000 Scopus - Multidisciplinary true 1000 true Q1 DIRECT true true Reference 59918420 /api/reference/59918420 1. Rasche, L., Paul, F., Ozanimod for the treatment of relapsing remitting multiple sclerosis (2018) Expert Opin. Pharmacother, 19, pp. 2073-2086. , 30407868 1 false true Reference 59918421 /api/reference/59918421 2. Lassiter, G., Ozanimod to treat relapsing forms of multiple sclerosis: a comprehensive review of disease, drug efficacy and side effects (2020) Neuro Int, 12, pp. 89-108 2 false true Reference 59918422 /api/reference/59918422 3. Fronza, M., Lorefice, L., Frau, J., Cocco, E., An overview of the efficacy and safety of Ozanimod for the treatment of relapsing multiple sclerosis (2021) Drug Des. Dev. Ther, 15, pp. 1993-2004 3 false true Reference 59918423 /api/reference/59918423 4. Lamb, Y.N., Ozanimod: First approval (2020) Drugs, 80, pp. 841-848 4 false true Reference 59918424 /api/reference/59918424 5. (2006), ICH Q3A—Impurities in New Drug Substances 5 false true Reference 59918425 /api/reference/59918425 6. (2006) ICH Q3B—Impurities in New Drug Products 6 false true Reference 59918426 /api/reference/59918426 7. Hancu, G., Modroiu, A., Chiral switch: between therapeutical benefit and marketing strategy (2022) Pharmaceuticals 2022, 15, p. 240 7 false true Reference 59918427 /api/reference/59918427 8. Cianferotti, C., Enantioselective synthesis of ozanimod, the active pharmaceutical ingredient of a new drug for multiple sclerosis (2021) Eur. J. Org. Chem, 2021, pp. 1924-1930 8 false true Reference 59918428 /api/reference/59918428 9. Horváth, S., Németh, G., Hysteresis of retention and enantioselectivity on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in mixtures of 2-propanol and methanol (2018) J. Chromatogr. A, 1568, pp. 149-159. , 30064816 9 false true Reference 59918429 /api/reference/59918429 10. Uthoff, F., Löwe, J., Harms, C., Donsbach, K., Gröger, H., Chemoenzymatic synthesis of a chiral ozanimod key intermediate starting from naphthalene as cheap petrochemical feedstock (2019) J. Org. Chem, 84, pp. 4856-4866. , 30977649 10 false true Reference 59918430 /api/reference/59918430 11. Porcs-Makkay, M., (2018), Process for the Production of Ozanimod—Patent No. WO2018/215807A1 11 false true Reference 59918431 /api/reference/59918431 12. Buchlovic, M., Chalupova, L., Vyklicky, L., Bartl, J., (2019), Process for Preparing Ozanimod—Patent No. US11390593 B2 12 false true Reference 59918432 /api/reference/59918432 13. Papp, L.A., Szabó, Z.I., Hancu, G., Farczádi, L., Mircia, E., Comprehensive review on chiral stationary phases in single-column simultaneous chiral–achiral HPLC separation methods (2024) Molecules, 29, p. 1346. , 38542982, 10975973 13 false true Reference 59918433 /api/reference/59918433 14. Liu, H., Wu, Z., Chen, J., Wang, J., Qiu, H., Recent advances in chiral liquid chromatography stationary phases for pharmaceutical analysis (2023) J. Chromatogr. A, 1708, p. 464367. , 37714014 14 false true Reference 59918434 /api/reference/59918434 15. Dallocchio, R., Dessì, A., Sechi, B., Peluso, P., Molecular dynamics simulations of amylose- and cellulose-based selectors and related enantioseparations in liquid phase chromatography (2023) Molecules, 28, p. 7419. , 37959839, 10647714 15 false true Reference 59918435 /api/reference/59918435 16. Sechi, B., Unravelling dispersion forces in liquid-phase enantioseparation. Part I: impact of ferrocenyl versus phenyl groups (2023) Anal. Chim. Acta, 1278, p. 341725. , 37709466 16 false true Reference 59918436 /api/reference/59918436 17. Peluso, P., Comparative enantioseparation of chiral 4,4’-bipyridine derivatives on coated and immobilized amylose-based chiral stationary phases (2020) J. Chromatogr. A, 1625, p. 461303. , 32709346 17 false true Reference 59918437 /api/reference/59918437 18. Ferencz, E., The applicability of chromatographic retention modeling on chiral stationary phases in reverse-phase mode: a case study for ezetimibe and its impurities (2023) Int. J. Mol. Sci, 24, p. 16097. , 38003286, 10671152 18 false true Reference 59918438 /api/reference/59918438 19. Sousa, E.P., Enantiomeric resolution of kielcorin derivatives by HPLC on polysaccharide stationary phases using multimodal elution (2004) Chirality, 16, pp. 279-285. , 15069657 19 false true Reference 59918439 /api/reference/59918439 20. Nistor, I., Implementation of a design space approach for enantiomeric separations in polar organic solvent chromatography (2013) J. Pharm. Biomed. Anal, 74, pp. 273-283. , 23245261 20 false true Reference 59918440 /api/reference/59918440 21. Chankvetadze, B., Recent trends in preparation, investigation and application of polysaccharide-based chiral stationary phases for separation of enantiomers in high-performance liquid chromatography (2020) Trends Anal. Chem, 122, p. 115709 21 false true Reference 59918441 /api/reference/59918441 22. Matarashvili, I., High-performance liquid chromatographic separations of stereoisomers of chiral basic agrochemicals with polysaccharide-based chiral columns and polar organic mobile phases (2015) J. Sep. Sci, 38, pp. 4173-4179. , 26497314 22 false true Reference 59918442 /api/reference/59918442 23. Szabó, Z.I., Chiral separation of lenalidomide by liquid chromatography on polysaccharide-type stationary phases and by capillary electrophoresis using cyclodextrin selectors (2018) J. Sep. Sci, 41, pp. 1414-1423. , 29211341 23 false true Reference 59918443 /api/reference/59918443 24. Németi, G., High-performance liquid chromatographic enantioseparation of azole analogs of monoterpene lactones and amides focusing on the separation characteristics of polysaccharide-based chiral stationary phases (2024) J. Chromatogr. A, 1717, p. 464660. , 38280361 24 false true Reference 59918444 /api/reference/59918444 25. Dobó, M., Foroughbakhshfasaei, M., Horváth, P., Szabó, Z.I., Tóth, G., Chiral separation of oxazolidinone analogues by liquid chromatography on polysaccharide stationary phases using polar organic mode (2022) J. Chromatogr. A, 1662, p. 462741. , 34929572 25 false true Reference 59918445 /api/reference/59918445 26. Horváth, S., Thuy, N., Eke, H.H., Németh, G., Exploitation of the enantioselectivity space of coated amylose tris(3,5-dimethylphenylcarbamate) in mixtures of 2-propanol and acetonitrile (2023) J. Chromatogr. A, 1705, p. 464161. , 37352691 26 false true Reference 59918446 /api/reference/59918446 27. Jadhav, S.B., Mane, R.M., Narayanan, K.L., Bhosale, P.N., Analytical enantio-separation of linagliptin in linagliptin and metformin HCl dosage forms by applying two-level factorial design (2016) Sci. Pharm, 84, pp. 671-684. , 27763526, 5198026 27 false true Reference 59918447 /api/reference/59918447 28. Papp, L.A., Hancu, G., Szabó, Z.I., Simultaneous determination of enantiomeric and organic impurities of vildagliptin on a cellulose tris(3-chloro-4-methylphenylcarbamate) column under revered-phase conditions (2023) J. Pharm. Biomed. Anal, 234, p. 115495. , 37343452 28 false true Reference 59918448 /api/reference/59918448 29. Ferencz, E., Simultaneous determination of chiral and achiral impurities of ivabradine on a cellulose tris(3-chloro-4-methylphenylcarbamate) chiral column using polar organic mode (2020) J. Pharm. Biomed. Anal, 177, p. 112851. , 31499427 29 false true Reference 59918449 /api/reference/59918449 30. Wadie, M., Abdel-Moety, E.M., Rezk, M.R., Tantawy, M.A., Eco-friendly chiral HPLC method for determination of alfuzosin enantiomers and solifenacin in their newly pharmaceutical combination: method optimization via central composite design (2021) Microchem. J, 165. , & 30 false true Reference 59918450 /api/reference/59918450 31. Dobó, M., Simultaneous determination of enantiomeric purity and organic impurities of dexketoprofen using reversed-phase liquid chromatography—enhancing enantioselectivity through hysteretic behavior and temperature-dependent enantiomer elution order reversal on polysaccharide chiral stationary phases (2024) Int. J. Mol. Sci, 25, p. 2697. , 38473945, 10932108 31 false true Reference 59918451 /api/reference/59918451 32. Bobály, B., D’Atri, V., Beck, A., Guillarme, D., Fekete, S., Analysis of recombinant monoclonal antibodies in hydrophilic interaction chromatography: a generic method development approach (2017) J. Pharm. Biomed. Anal, 145, pp. 24-32. , 28646659 32 false true Reference 59918452 /api/reference/59918452 33. Baczek, T., Kaliszan, R., Claessens, H., van Straten, M., Computer-assisted optimization of reversed-phase HPLC isocratic separations of neutral compounds (2001) LCGC Europe, pp. 2-6 33 false true Reference 59918453 /api/reference/59918453 34. (2022) Monography 1220: Analytical procedures life cycle, 1. , In United States Pharmacopeia—National Formulary 34 false true Reference 59918454 /api/reference/59918454 35. Snyder, L.R., Dolan, J.W., Lommen, D.C., Drylab® computer simulation for high-performance liquid chromatographic method development: I. Isocratic elution (1989) J. Chromatogr. A, 485, pp. 65-89 35 false true Reference 59918455 /api/reference/59918455 36. Dolan, J.W., Lommen, D.C., Snyder, L.R., Drylab® computer simulation for high-performance liquid chromatographic method development: II. Gradient elution (1989) J. Chromatogr. A, 485, pp. 91-112 36 false true Reference 59918456 /api/reference/59918456 37. Larew, L.A., Olsen, B.A., Stafford, J.D., Wilhelm, M.V., Comparison of theory-based and empirical modeling for the prediction of chromatographic behavior in the ion-pairing separation of benzodiazepine-derived pharmaceutical compounds (1995) J. Chromatogr. A, 692, pp. 183-193 37 false true Reference 59918457 /api/reference/59918457 38. Fekete, S., Beck, A., Fekete, J., Guillarme, D., Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, part I: salt gradient approach (2015) J. Pharm. Biomed. Anal, 102, pp. 33-44. , 25240157 38 false true Reference 59918458 /api/reference/59918458 39. Fekete, S., Beck, A., Fekete, J., Guillarme, D., Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, part II: pH gradient approach (2015) J. Pharm. Biomed. Anal, 102, pp. 282-289. , 25459925 39 false true Reference 59918459 /api/reference/59918459 40. D’Atri, V., Pell, R., Clarke, A., Guillarme, D., Fekete, S., Is hydrophobic interaction chromatography the most suitable technique to characterize site-specific antibody-drug conjugates? (2019) J. Chromatogr. A, 1586, pp. 149-153. , 30554777 40 false true Reference 59918460 /api/reference/59918460 41. Neumann, J., Schmidtsdorff, S., Schmidt, A.H., Parr, M.K., Retention modeling of therapeutic peptides in sub-/supercritical fluid chromatography (2024) Sep. Sci. Plus, 7, p. 2300239 41 false true Reference 59918461 /api/reference/59918461 42. Lämmerhofer, M., Di Eugenio, P., Molnar, I., Lindner, W., Computerized optimization of the high-performance liquid chromatographic enantioseparation of a mixture of 4-dinitrophenyl amino acids on a quinine carbamate-type chiral stationary phase using drylab (1997) J. Chromatogr. B Biomed. Sci. Appl, 689, pp. 123-135. , 9061488 42 false true Reference 59918462 /api/reference/59918462 43. Zhang, L., Hu, Y., Galella, E., Tomasella, F.P., Fish, W.P., Separation of atropisomers by chiral liquid chromatography and thermodynamic analysis of separation mechanism (2017) J. Pharm. Anal, 7, pp. 156-162. , 29404032, 5790684 43 false true Reference 59918463 /api/reference/59918463 44. Wagdy, H.A., Hanafi, R.S., El-Nashar, R.M., Aboul-Enein, H.Y., Predictability of enantiomeric chromatographic behavior on various chiral stationary phases using typical reversed phase modeling software (2013) Chirality, 25, pp. 506-513. , 23775938 44 false true Reference 59918464 /api/reference/59918464 45. (2022) ICH Q14—Analytical Procedure Development 45 false true Reference 59918465 /api/reference/59918465 46. (2005) ICH Q9(R1)—Quality Risk Management 46 false true Reference 59918466 /api/reference/59918466 47. Fekete, S., Molnár, I., Rieger, H.J., Kormány, R., HPLC method development by QbD compatible resolution modeling (DryLab4) (2018) In Software-Assisted Method Development in High Performance Liquid Chromatography, World Science, pp. 11-51. , (Europe) 47 false true Reference 59918467 /api/reference/59918467 48. Kormány, R., Molnár, I., Fekete, J., Guillarme, D., Fekete, S., Robust UHPLC separation method development for multi-API product containing amlodipine and bisoprolol: the impact of column selection (2014) Chromatographia, 77, pp. 1119-1127 48 false true /api/publication/35578246 Ferencz E. et al. Possibilities and limitations of computer assisted chiral HPLC method development for ozanimod on polysaccharide based chiral stationary phases. (2024) SCIENTIFIC REPORTS 2045-2322 14 1<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Ferencz E. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Szabó Z.-I.^* </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zöldhegyi A. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10087450"><a href="/gui2/?type=authors&mode=browse&sel=10087450" target="_blank">Dombi G. (<span class="authorship-author-name">Dombi Gergely</span> <span class="authorAux-mtmt"> Gyógyszeranalitika, HPLC</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ; <span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10100801"><a href="/gui2/?type=authors&mode=browse&sel=10100801" target="_blank">Molnár G. (<span class="authorship-author-name">Molnár Gergely</span> <span class="authorAux-mtmt"> Királis nagyhatékonyságú folyadékkromatográfia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10081854"><a href="/gui2/?type=authors&mode=browse&sel=10081854" target="_blank">Dobó M. (<span class="authorship-author-name">Dobó Máté</span> <span class="authorAux-mtmt"> elválasztástechnika, (királis HPLC)</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Varga E. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Molnár I. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10028950"><a href="/gui2/?type=authors&mode=browse&sel=10028950" target="_blank">Tóth G. ✉ (<span class="authorship-author-name">Tóth Gergő</span> <span class="authorAux-mtmt"> gyógyszerkémia/analitika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ; <span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35578246" target="_blank">Possibilities and limitations of computer assisted chiral HPLC method development for ozanimod on polysaccharide based chiral stationary phases</a></div> <div> <span class="journal-title">SCIENTIFIC REPORTS</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2045-2322">2045-2322</a>)</span>: <span class="journal-volume">14</span> <span class="journal-issue">1</span> <span class="page"> Paper 26757. 12 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1038/s41598-024-78415-1" target="_blank" href="https://doi.org/10.1038/s41598-024-78415-1"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001348897100032" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001348897100032"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85208602910" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85208602910"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="39500751" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39500751&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="https://www.nature.com/articles/s41598-024-78415-1" target="_blank" href="https://www.nature.com/articles/s41598-024-78415-1"> Egyéb URL </a> </span> <span class="id identifier oa_NONE" title=" Nincs "> <a style="color:blue" title="https://www.scopus.com/pages/publications/85208602910?origin=resultslist" target="_blank" href="https://www.scopus.com/pages/publications/85208602910?origin=resultslist"> Egyéb URL </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Multidisciplinary&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Szociológiai Tudományos Bizottság IXGJO SZTB [1901-]&nbsp;&nbsp;&nbsp;A nemzetközi</div> <div class="journal-subject">Regionális Tudományok Bizottsága IXGJO RTB [1901-]&nbsp;&nbsp;&nbsp;B nemzetközi</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 3</span> | Független: 2 | Függő: 1 | Nem jelölt: 0 | WoS jelölt: 3 | Scopus jelölt:&nbsp;3 | WoS/Scopus jelölt:&nbsp;3 | DOI jelölt:&nbsp;3 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35578246&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 11 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35578246</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="funder"> (FK 146930), Nemzeti Kutatási, Fejlesztési és Innovációs Alap(ÚNKP-23-3-II), (ÚNKP-23-2-III), (EKÖP-2024-57), (János Bolyai Research Scholarship) </div> <div class="lastModified">Utolsó módosítás: 2026.04.22. 11:25 Szalóky-Siki Ágnes (SE_KK_Admin5_SZSA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Export Date: 22 April 2026; Cited By: 3; Correspondence Address: G. Tóth; Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hőgyes E. u. 9, 1092, Hungary; email: toth.gergo@semmelweis.hu;</pre> </div></div>