CardiLect: A combined cross‐species lectin histochemistry protocol for the automated analysis of cardiac remodelling

https://m2.mtmt.hu/ eng 2026-04-16 13:43 JournalArticle 35562030 VALIDATED true Export Date: 23 June 2025; Correspondence Address: Z.V. Varga; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary; email: varga.zoltan@semmelweis.hu 0 false 2026-03-17T12:27:43.690+0000 2026-03-16T10:19:24.042+0000 2024-11-13T13:55:29.065+0000 true 10071323 Mórotz Gábor Miklós /api/author/10071323 Author Gábor Miklós Mórotz (Molekuláris sejtbiológia) true 2026-03-27T10:21:16.153+0000 2026-03-27T10:21:16.153+0000 2025-05-15T08:58:06.960+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin Tamásné Gere (SE_KK_Admin5_GT, admin) true 10062062 true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Gergely, Tamás G. CardiLect: A combined cross‐species lectin histochemistry protocol for the automated analysis of cardiac remodelling true true /api/journal/10035112 REVIEWED ESC HEART FAILURE 2055-5822 2055-5822 true false 10035112 false 10035112 true 2055-5822 2055-5822 Journal FOREIGN 12 2 1398 1415 1398 18 2025 Cardiac remodelling, a crucial aspect of heart failure, is commonly investigated in preclinical models by quantifying cardiomyocyte cross-sectional area (CSA) and microvascular density (MVD) via histological methods, such as immunohistochemistry. To achieve this, optimized protocols are needed, and the species specificity is dependent on the antibody used. Lectin histochemistry offers several advantages compared to antibody-based immunohistochemistry, including as cost-effectiveness and cross-species applicability. Direct comparisons between the two methods are lacking from the literature.In this study, we compared antibody- and lectin-based methods for the histological assessment of cardiomyocyte CSA (with the use of anti-laminin and wheat germ agglutinin [WGA]) and microvascular density (utilizing anti-CD31 and isolectin B4 [ILB4]) using different embedding and antigen/carbohydrate retrieval techniques. Here, we describe a detailed, easy-to-use combined lectin histochemistry protocol (WGA and ILB4, 'CardiLect' protocol) for the histological assessment of cardiac remodelling. The lectin-based approach has been evaluated on a cross-species basis, and its efficacy has been demonstrated in zebrafish, rodents, large animals and human samples. We provide an ImageJ script ('CardiLect Analyser') for automated image analysis, validated in a preclinical heart failure model by correlating histological parameters with echocardiographic findings. CSA showed a significant positive correlation with left ventricular (LV) mass (P = 0.0098, rS = 0.7545) and significant negative correlation with markers of systolic function, such as ejection fraction (EF) (P = 0.0402, rS = -0.6364). Microvascular density showed significant negative correlation with LV mass (P = 0.0055, rS = -0.7622) and significant positive correlation with EF (P = 0.0106, rS = 0.7203).The described combined lectin histochemistry protocol with the provided ImageJ script is an easy-to-use, cost-effective, cross-species approach for the histological assessment of cardiac remodelling. Funding 2046579 /api/funding/2046579 National Research, Development and Innovation Office (NKFIH) of Hungary(FK134751) false true Funding 2046580 /api/funding/2046580 Thematic Excellence Program (Semmelweis University)(2020-4.1.1.-TKP2020) Funder: Ministry for Innovation and Technology false true Funding 2046581 /api/funding/2046581 (ÚNKP-23-4-I-SE-5) false true Funding 2046582 /api/funding/2046582 (ÚNKP-23-II-SE-43) false true Funding 2046583 /api/funding/2046583 (ÚNKP-23-5-SE-9) false true Funding 2046584 /api/funding/2046584 (BO/00277/23/5) false true Funding 2047076 /api/funding/2047076 (RRF-2.3.1-21-2022-00003) false true Funding 2047077 /api/funding/2047077 (FK-146163) false true true 2024 true true true false true false GOLD 2026-03-17 true http://real.mtak.hu/225018 5 0 3 2 2 3 4 1 2 2 2 1 1 3 2 0 5 3 3 2 0 9 9 Folyóiratcikk Journal Article 24 3 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 1 Egyéb Miscellaneous 29 1 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 4 2 4 2 2026 0 1 1 1 1 Q1 false 10074629 false false false 2025-02-11T13:55:29.612+0000 Biofizikai és Sugárbiológiai Intézet (SE / AOK / I); Farmakológiai és Farmakoterápiás Intézet (SE / AOK / I); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); HCEMM-SE Gyulladásos Jelátviteli Kutatócsoport (SE / AOK / I / ÉI); HCEMM-SE Kardiometabolikus Immunológia Kutatócs... (SE / AOK / I / FFI); MTA-SE Lendület Szöveti Sérülés Kutatócsoport (SE / AOK / I / ÉI); MTA-SE Momentum Kardio-Onkológia és Kardioimmun... (SE / AOK / I / FFI); Élettani Intézet (SE / AOK / I) 14 34 8 15 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 121550097 /api/authorship/121550097 Gergely, Tamás G. [Gergely, Tamás G (kardiovaszkuláris...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... (SU / FM / I / DPP) 1 0.067 true false false Author 10074629 /api/author/10074629 Tamás G Gergely (kardiovaszkuláris farmakológia) Gergely Tamás G true true Gergely Tamás G. true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550098 /api/authorship/121550098 Kovács, Tamás* [Kovács, Tamás (Molekuláris orvos...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... (SU / FM / I / DPP) 2 0.067 true false false Author 10060030 /api/author/10060030 Tamás Kovács (Molekuláris orvostudományok) Kovács Tamás true 10060030 true Kovács Tamás true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550099 /api/authorship/121550099 Kovács, Andrea [Kovács, Andrea (hisztológia, immu...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... (SU / FM / I / DPP) 3 0.067 false false false Author 10091976 /api/author/10091976 Andrea Kovács (hisztológia, immunológia, kardiológia) Kovács Andrea true true Kovács Andrea true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550100 /api/authorship/121550100 Tóth, Viktória E. [Tóth, Viktória (Farmakológia), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... (SU / FM / I / DPP) 4 0.067 false false false Author 10040905 /api/author/10040905 Viktória Tóth (Farmakológia) Tóth Viktória true 10040905 true Tóth Viktória E. true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550101 /api/authorship/121550101 Sayour, Nabil V. [Sayour, Viktor Nabil (farmakológia), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... 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(SU / FM / I / DPP) 9 0.067 false false false Author 10061054 /api/author/10061054 Zsófia Onódi (Farmakológia) Onódi Zsófia true 10061054 true Onódi Zsófia true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550106 /api/authorship/121550106 Enyedi, Balázs [Enyedi, Balázs (élettan, molekulá...), author] Department of Physiology (SU / FM / I); HCEMM-SU Inflammatory Signaling Research Group (SU / FM / I / DP); MTA-SE Lendület Tissue Damage Research Group (SU / FM / I / DP) 10 0.067 false false false Author 10023424 /api/author/10023424 Balázs Enyedi (élettan, molekuláris biológia) Enyedi Balázs true 10023424 true Enyedi Balázs true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550107 /api/authorship/121550107 Máthé, Domokos [Máthé, Domokos (Molekuláris képal...), author] Departmnet of Biophysics and Radiation Biology (SU / FM / I) 11 0.067 false false false Author 10054788 /api/author/10054788 Domokos Máthé (Molekuláris képalkotás) Máthé Domokos true 10054788 true Máthé Domokos true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550108 /api/authorship/121550108 Leszek, Przemyslaw 12 0.06666667 false false false Leszek Przemyslaw true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550109 /api/authorship/121550109 Giricz, Zoltán [Giricz, Zoltán (Biokémia, Farmako...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE) 13 0.067 false false false Author 10026898 /api/author/10026898 Zoltán Giricz (Biokémia, Farmakológia) Giricz Zoltán true 10026898 true Giricz Zoltán true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550110 /api/authorship/121550110 Ferdinandy, Péter [Ferdinandy, Péter (Farmakológia, mol...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE) 14 0.067 false false false Author 10010125 /api/author/10010125 Péter Ferdinandy (Farmakológia, molekuláris medicina) Ferdinandy Péter true 10010125 true Ferdinandy Péter true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false true PersonAuthorship 121550111 /api/authorship/121550111 Varga, Zoltán V. ✉ [Varga, Zoltán (kardiovaszkuláris...), author] Department of Pharmacology and Pharmacotherapy (SU / FM / I); Centre for Pharmacology and Pharmaceutical Rese... (SU / KSZE); HCEMM-SU Cardiometabolic Immunology Research Group (SU / FM / I / DPP); MTA-SE Momentum Cardio-Oncology and Cardioimmun... 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DOI: 10.1007/BF00737716 38 10.1007/BF00737716 false true Reference 59775481 /api/reference/59775481 39. DOI: 10.1002/prca.201200014 39 10.1002/prca.201200014 false true /api/publication/35562030 Gergely Tamás G. et al. CardiLect: A combined cross‐species lectin histochemistry protocol for the automated analysis of cardiac remodelling. (2025) ESC HEART FAILURE 2055-5822 2055-5822 12 2 1398-1415<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <a href="/gui2/?type=authors&mode=browse&sel=10074629" target="_blank">Gergely Tamás G. (Gergely Tamás G kardiovaszkuláris farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10060030" target="_blank">Kovács Tamás* (Kovács Tamás Molekuláris orvostudományok) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10091976" target="_blank">Kovács Andrea (Kovács Andrea hisztológia, immunológia, kardiológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10040905" target="_blank">Tóth Viktória E. (Tóth Viktória Farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10074459" target="_blank">Sayour Nabil V. (Sayour Viktor Nabil farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10071323" target="_blank">Mórotz Gábor M. (Mórotz Gábor Miklós Molekuláris sejtbiológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10069239" target="_blank">Kovácsházi Csenger (Kovácsházi Csenger Gyógyszertudomány) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development ;    <a href="/gui2/?type=authors&mode=browse&sel=10052112" target="_blank">Brenner Gábor B. (Brenner Gábor Kardiovaszkuláris farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development ;    <a href="/gui2/?type=authors&mode=browse&sel=10061054" target="_blank">Onódi Zsófia (Onódi Zsófia Farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10023424" target="_blank">Enyedi Balázs (Enyedi Balázs élettan, molekuláris biológia) </a> SU/FM/I/Department of Physiology; SU/FM/I/DP/HCEMM-SU Inflammatory Signaling Research Group; SU/FM/I/DP/MTA-SE Lendület Tissue Damage Research Group ;    <a href="/gui2/?type=authors&mode=browse&sel=10054788" target="_blank">Máthé Domokos (Máthé Domokos Molekuláris képalkotás) </a> SU/FM/I/Departmnet of Biophysics and Radiation Biology ;    Leszek Przemyslaw ;    <a href="/gui2/?type=authors&mode=browse&sel=10026898" target="_blank">Giricz Zoltán (Giricz Zoltán Biokémia, Farmakológia) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development ;    <a href="/gui2/?type=authors&mode=browse&sel=10010125" target="_blank">Ferdinandy Péter (Ferdinandy Péter Farmakológia, molekuláris medicina) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development ;    <a href="/gui2/?type=authors&mode=browse&sel=10028393" target="_blank">Varga Zoltán V. ✉ (Varga Zoltán kardiovaszkuláris kutatás) </a> SU/FM/I/Department of Pharmacology and Pharmacotherapy; SU/KSZE/Centre for Pharmacology and Pharmaceutical Research Development; SU/FM/I/DPP/HCEMM-SU Cardiometabolic Immunology Research Group; SU/FM/I/DPP/MTA-SE Momentum Cardio-Oncology and Cardioimmunology Research Group </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35562030" target="_blank">CardiLect: A combined cross‐species lectin histochemistry protocol for the automated analysis of cardiac remodelling</a></div> <div> ESC HEART FAILURE (<a target="_blank" href="https://portal.issn.org/resource/ISSN/2055-5822">2055-5822</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/2055-5822">2055-5822</a>): 12 2 pp 1398-1415 (2025) </div> <div class="pub-footer"> Language: English | <a style="color:blue" title="10.1002/ehf2.15155" target="_blank" href="https://doi.org/10.1002/ehf2.15155"> DOI </a> <a style="color:blue" title="001356962300001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001356962300001"> WoS </a> <a style="color:black" title="225018" target="_blank" href="http://real.mtak.hu/225018"> REAL </a> <a style="color:blue" title="85208960685" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85208960685"> Scopus </a> <a style="color:blue" title="39535377" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39535377&dopt=Abstract"> PubMed </a> <a style="color:blue" title="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85208960685&doi=10.1002%2Fehf2.15155&partnerID=40&md5=080359c0d4eeded9b2a74a1f704bd77d" target="_blank" href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85208960685&doi=10.1002%2Fehf2.15155&partnerID=40&md5=080359c0d4eeded9b2a74a1f704bd77d"> Other URL </a> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Cardiology and Cardiovascular Medicine   Rank: Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> Citing papers: 5 | Independent citation: 3 | Self citation: 2 | Unknown citation: 0 | Number of citations in WoS: 1 | Number of citations in Scopus: 2 | WoS/Scopus assigned: 2 | Number of citations with DOI: 4 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35562030&sort=publishedYear,desc&sort=title"> Number of cited publications: 9 </a> </div> <div class="mtid">Publication: 35562030 | Validated Core Citing | Journal Article ( Article ) | Scientific | kézi felvitel </div> <div class="funder"> National Research, Development and Innovation Office (NKFIH) of Hungary(FK134751), Thematic Excellence Program (Semmelweis University)(2020-4.1.1.-TKP2020) Funder: Ministry for Innovation and Technology, (ÚNKP-23-4-I-SE-5), (ÚNKP-23-II-SE-43), (ÚNKP-23-5-SE-9), (BO/00277/23/5), (RRF-2.3.1-21-2022-00003), (FK-146163) </div> <div class="lastModified">Last Modified: 2026.03.16. 11:19 Márta Molnár-Taga (SE 4-es admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;">Comments: Export Date: 23 June 2025; Correspondence Address: Z.V. Varga; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary; email: varga.zoltan@semmelweis.hu</pre> </div></div>