https://m2.mtmt.hu/ hun 2026-09-13 07:40 JournalArticle 35469968 VALIDATED true Virology, Department of Infectious Diseases, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, 69120, Germany European Molecular Biology Laboratory, Heidelberg, 69117, Germany German Center for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, 69120, Germany Department of Internal Medicine IV, University Hospital Heidelberg, Heidelberg, 69120, Germany Institute of Computer Science, Göttingen University, Göttingen, 37073, Germany Cluster of Excellence ‘Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells’ (MBExC), Göttingen University, Göttingen, 37073, Germany Export Date: 18 October 2024 Correspondence Address: Laketa, V.; Virology, Germany; email: vibor.laketa@med.uni-heidelberg.de 0 false 2026-03-09T14:13:37.790+0000 2026-03-09T13:01:47.168+0000 2024-10-21T06:53:41.466+0000 true 10045852 Patkósné Hanesz Andrea /api/admin/10045852 Admin true 10045852 2026-03-09T13:01:47.614+0000 2026-03-09T13:01:47.614+0000 2025-02-06T15:55:43.911+0000 true 10095912 Hódi Adrienn /api/admin/10095912 Admin true 2025-03-17T01:59:02.038+0000 true 521 Szuper Admin /api/admin/521 Admin true false false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Sartingen, N. Multiplex Microscopy Assay for Assessment of Therapeutic and Serum Antibodies against Emerging Pathogens true true /api/journal/10015873 REVIEWED true false 10015873 false 10015873 true 1999-4915 Journal FOREIGN 16 9 1473 1473 2024 The emergence of novel pathogens, exemplified recently by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the need for rapidly deployable and adaptable diagnostic assays to assess their impact on human health and guide public health responses in future pandemics. In this study, we developed an automated multiplex microscopy assay coupled with machine learning-based analysis for antibody detection. To achieve multiplexing and simultaneous detection of multiple viral antigens, we devised a barcoding strategy utilizing a panel of HeLa-based cell lines. Each cell line expressed a distinct viral antigen, along with a fluorescent protein exhibiting a unique subcellular localization pattern for cell classification. Our robust, cell segmentation and classification algorithm, combined with automated image acquisition, ensured compatibility with a high-throughput approach. As a proof of concept, we successfully applied this approach for quantitation of immunoreactivity against different variants of SARS-CoV-2 spike and nucleocapsid proteins in sera of patients or vaccinees, as well as for the study of selective reactivity of monoclonal antibodies. Importantly, our system can be rapidly adapted to accommodate other SARS-CoV-2 variants as well as any antigen of a newly emerging pathogen, thereby representing an important resource in the context of pandemic preparedness. © 2024 by the authors. 2024 true true true false false false GOLD 2026-03-09 false http://www.mdpi.com/journal/viruses 0 0 0 0 0 0 0 0 0 0 0 0 2 2 Q1 false false false false 2025-01-19T07:54:01.541+0000 16 57 0 12 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 121011180 /api/authorship/121011180 1 0.083333336 true false false Sartingen N. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011181 /api/authorship/121011181 2 0.083333336 false false false Stürmer V. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011182 /api/authorship/121011182 3 0.083333336 false false false Kaltenböck M. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011183 /api/authorship/121011183 4 0.083333336 false false false Müller T.G. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011184 /api/authorship/121011184 5 0.083333336 false false false Schnitzler P. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011185 /api/authorship/121011185 6 0.083333336 false false false Kreshuk A. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011186 /api/authorship/121011186 7 0.083333336 false false false Kräusslich H.-G. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011187 /api/authorship/121011187 8 0.083333336 false false false Merle U. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011188 /api/authorship/121011188 9 0.083333336 false false false Mücksch F. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011189 /api/authorship/121011189 10 0.083333336 false false false Müller B. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011190 /api/authorship/121011190 11 0.083333336 false false false Pape C. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 121011191 /api/authorship/121011191 12 0.083333336 false true true Laketa V. true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 27670201 /api/publicationidentifier/27670201 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true IDENTICAL 10.3390/v16091473 https://doi.org/10.3390/v16091473 false true PublicationIdentifier 27670202 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class="author-name" >Schnitzler P. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kreshuk A. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kräusslich H.-G. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Merle U. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mücksch F. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Müller B. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Pape C. </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Laketa V. ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35469968" target="_blank">Multiplex Microscopy Assay for Assessment of Therapeutic and Serum Antibodies against Emerging Pathogens</a></div> <div> <span class="journal-title">VIRUSES</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/1999-4915">1999-4915</a>)</span>: <span class="journal-volume">16</span> <span class="journal-issue">9</span> <span class="page"> Paper 1473. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.3390/v16091473" target="_blank" href="https://doi.org/10.3390/v16091473"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001323086000001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001323086000001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85205065459" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85205065459"> Scopus </a> </span> </span> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35469968&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 2 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35469968</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">Scopus</span> </div> <div class="lastModified">Utolsó módosítás: 2026.03.09. 14:01 Vigyikánné Váradi Anikó (SZTE admin5 SZAOK Orvosi Mikrobiológiai Intézet) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Virology, Department of Infectious Diseases, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, 69120, Germany European Molecular Biology Laboratory, Heidelberg, 69117, Germany German Center for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, 69120, Germany Department of Internal Medicine IV, Uni...</pre> </div></div>