mtmt
The Hungarian Scientific Bibliography
XML
JSON
Public search
Magyarul
Multiplex Microscopy Assay for Assessment of Therapeutic and Serum Antibodies against Emerging Pathogens
Sartingen, N.
;
Stürmer, V.
;
Kaltenböck, M.
;
Müller, T.G.
;
Schnitzler, P.
;
Kreshuk, A.
;
Kräusslich, H.-G.
;
Merle, U.
;
Mücksch, F.
;
Müller, B.
;
Pape, C.
;
Laketa, V. ✉
English Article (Journal Article) Scientific
Published:
VIRUSES 1999-4915
16
(9)
Paper: 1473
2024
SJR Scopus - Infectious Diseases: Q1
Identifiers
MTMT: 35469968
DOI:
10.3390/v16091473
WoS:
001323086000001
Scopus:
85205065459
The emergence of novel pathogens, exemplified recently by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the need for rapidly deployable and adaptable diagnostic assays to assess their impact on human health and guide public health responses in future pandemics. In this study, we developed an automated multiplex microscopy assay coupled with machine learning-based analysis for antibody detection. To achieve multiplexing and simultaneous detection of multiple viral antigens, we devised a barcoding strategy utilizing a panel of HeLa-based cell lines. Each cell line expressed a distinct viral antigen, along with a fluorescent protein exhibiting a unique subcellular localization pattern for cell classification. Our robust, cell segmentation and classification algorithm, combined with automated image acquisition, ensured compatibility with a high-throughput approach. As a proof of concept, we successfully applied this approach for quantitation of immunoreactivity against different variants of SARS-CoV-2 spike and nucleocapsid proteins in sera of patients or vaccinees, as well as for the study of selective reactivity of monoclonal antibodies. Importantly, our system can be rapidly adapted to accommodate other SARS-CoV-2 variants as well as any antigen of a newly emerging pathogen, thereby representing an important resource in the context of pandemic preparedness. © 2024 by the authors.
Citing (1)
Citation styles:
IEEE
ACM
APA
Chicago
Harvard
CSL
Copy
Print
2025-02-18 14:01
×
Export list as bibliography
Citation styles:
IEEE
ACM
APA
Chicago
Harvard
Print
Copy