https://m2.mtmt.hu/ hun 2026-08-26 06:22 JournalArticle 35429645 VALIDATED true Funding Agency and Grant Number: National Research, Development, and Innovation Office, Hungary [NKFIH FK 146930]; National Research, Development, and Innovation fund; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; National Research, Development, and Innovation Fund (Hungary) [TKP2021-NVA-14]; [UNKP-23-3-II-SE-3]; [UNKP-23-2-III-SE-6]; [EKOEP-2024-57]; [UNKP-2023-3-II-PTE-1969] Funding text: This work was funded by the National Research, Development, and Innovation Office, Hungary (grant: NKFIH FK 146930). This work was supported by the UNKP-23-3-II-SE-3 (M.D.), UNKP-23-2-III-SE-6 and EKOEP-2024-57 (G.D.), and UNKP-2023-3-II-PTE-1969 (L.Ty.) projects of the New National Excellence Program of the Ministry for Culture and Innovation, funded by the National Research, Development, and Innovation fund. This work was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (G.T.). B.F. thanks the support by the National Research, Development, and Innovation Fund (Hungary) within the TKP2021-NVA-14 project. 0 false 2026-06-02T13:56:19.861+0000 2026-05-19T07:33:57.373+0000 2024-10-01T13:36:35.675+0000 true 10015206 Áncsán Gizella /api/admin/10015206 Admin Áncsán Gizella (SE admin) true 10015206 2026-06-02T13:52:06.586+0000 2025-03-14T09:01:35.676+0000 true 10080205 Szalóky-Siki Ágnes /api/admin/10080205 Admin Szalóky-Siki Ágnes (SE_KK_Admin5_SZSA, admin) true true false true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Folyóiratcikk 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Szakcikk (Folyóiratcikk) 101 true 10000059 true 1 /api/category/1 Category Tudományos true 1 Dombi, Gergely true true /api/journal/10003252 REVIEWED INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 true false 10003252 false 10003252 true 1661-6596 1422-0067 Journal FOREIGN 25 19 10575 10575 20 2024 The enantioselective binding of three proton pump inhibitors (PPIs)—omeprazole, rabeprazole, and lansoprazole—to two key plasma proteins, α1-acid glycoprotein (AGP) and human serum albumin (HSA), was characterized. The interactions between PPI enantiomers and proteins were investigated using a multifaceted analytical approach, including high-performance liquid chromatography (HPLC), fluorescence and UV spectroscopy, as well as in silico molecular docking. HPLC analysis demonstrated that all three PPIs exhibited enantioseparation on an AGP-based chiral stationary phase, suggesting stereoselective binding to AGP, while only lansoprazole showed enantioselective binding on the HSA-based column. Quantitatively, the S-enantiomers of omeprazole and rabeprazole showed higher binding affinity to AGP, while the R-enantiomer of lansoprazole displayed greater affinity for AGP, with a reversal in the elution order observed between the two protein-based columns. Protein binding percentages, calculated via HPLC, were greater than 88% for each enantiomer across both transport proteins, with all enantiomers displaying higher affinity for AGP compared to HSA. Thermodynamic analysis indicated that on the HSA, the more common, enthalpy-controlled enantioseparation was found, while in contrast, on the AGP, entropy-controlled enantioseparation was observed. The study also identified limitations in using fluorescence titration due to the high native fluorescence of the compounds, whereas UV titration was effective for both proteins. The determined logK values were in the range of 4.47–4.83 for AGP and 4.02–4.66 for HSA. Molecular docking supported the experimental findings by revealing the atomic interactions driving the binding process, with the predicted enantiomer elution orders aligning with experimental data. The comprehensive use of these analytical methods provides detailed insights into the enantioselective binding properties of PPIs, contributing to the understanding of their pharmacokinetic differences and aiding in the development of more effective therapeutic strategies. Funding 2045698 /api/funding/2045698 (FK 146930) false true Funding 2045699 /api/funding/2045699 (ÚNKP-23-3-II-SE-3) false true Funding 2045700 /api/funding/2045700 (ÚNKP-23-2-III-SE-6) false true Funding 2045701 /api/funding/2045701 (EKÖP-2024-57) false true true true 2024 true true true false true false GOLD 2026-06-02 true https://doi.org/10.3390/ijms251910575 10 0 10 8 2 10 10 9 9 10 10 8 8 8 2 0 10 8 10 8 0 7 7 Folyóiratcikk Journal Article 24 10 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2025 0 7 6 7 6 2026 0 3 2 3 2 D1 false false false false 2024-10-20T08:42:34.750+0000 Biológia és Kémia Tanszék (RE); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE); Gyógyszerészi Kémiai Intézet (PTE / GYTK); Gyógyszerészi Kémiai Intézet (SE / GYTK); Korszerű Anyagok és Intelligens Technológiák Fe... (ME / K); Kémiai Intézet (ME / AVK) 7 33 6 8 0 true Language 10002 /api/language/10002 Angol Angol English true 2 true PersonAuthorship 120723228 /api/authorship/120723228 Dombi, Gergely [Dombi, Gergely (Gyógyszeranalitik...), szerző] Gyógyszerészi Kémiai Intézet (SE / GYTK); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE) 1 0.125 true false false Author 10087450 /api/author/10087450 Dombi Gergely (Gyógyszeranalitika, HPLC) Dombi Gergely true true Dombi Gergely true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723229 /api/authorship/120723229 Tyukodi, Levente [Tyukodi, Levente (Gyógyszerészi kémia), szerző] Gyógyszerészi Kémiai Intézet (PTE / GYTK) 2 0.125 false false false Author 10076539 /api/author/10076539 Tyukodi Levente (Gyógyszerészi kémia) Tyukodi Levente true true Tyukodi Levente true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723230 /api/authorship/120723230 Dobó, Máté [Dobó, Máté (elválasztástechni...), szerző] Gyógyszerészi Kémiai Intézet (SE / GYTK); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE) 3 0.125 false false false Author 10081854 /api/author/10081854 Dobó Máté (elválasztástechnika, (királis HPLC)) Dobó Máté true true Dobó Máté true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723231 /api/authorship/120723231 Molnár, Gergely [Molnár, Gergely (Királis nagyhaték...), szerző] Gyógyszerészi Kémiai Intézet (SE / GYTK); Farmakológiai és Gyógyszerkutatás-fejlesztési K... (SE / KSZE) 4 0.125 false false false Author 10100801 /api/author/10100801 Molnár Gergely (Királis nagyhatékonyságú folyadékkromatográfia) Molnár Gergely true true Molnár Gergely true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723232 /api/authorship/120723232 Rozmer, Zsuzsanna [Rozmer, Zsuzsanna (Gyógyszerészi kémia), szerző] Gyógyszerészi Kémiai Intézet (PTE / GYTK) 5 0.125 false false false Author 10016687 /api/author/10016687 Rozmer Zsuzsanna (Gyógyszerészi kémia) Rozmer Zsuzsanna true 10016687 true Rozmer Zsuzsanna true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723233 /api/authorship/120723233 Szabó, Zoltán-István 6 0.125 false false false Szabó Zoltán-István true false false AuthorshipType 1 /api/authorshiptype/1 Szerző 0 true 0 true false true PersonAuthorship 120723234 /api/authorship/120723234 Fiser, Béla [Fiser, Béla (Számítógépes kémi...), szerző] Kémiai Intézet (ME / AVK); Biológia és Kémia Tanszék (RE); Korszerű Anyagok és Intelligens Technológiák Fe... 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Enantioselective Binding of Proton Pump Inhibitors to Alpha1-Acid Glycoprotein and Human Serum Albumin—A Chromatographic, Spectroscopic, and In Silico Study. (2024) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 25 19<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10087450"><a href="/gui2/?type=authors&mode=browse&sel=10087450" target="_blank">Dombi Gergely (<span class="authorship-author-name">Dombi Gergely</span> <span class="authorAux-mtmt"> Gyógyszeranalitika, HPLC</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10076539"><a href="/gui2/?type=authors&mode=browse&sel=10076539" target="_blank">Tyukodi Levente (<span class="authorship-author-name">Tyukodi Levente</span> <span class="authorAux-mtmt"> Gyógyszerészi kémia</span>) </a> </span> <span class="author-affil"><span title="Pécsi Tudományegyetem">PTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10081854"><a href="/gui2/?type=authors&mode=browse&sel=10081854" target="_blank">Dobó Máté (<span class="authorship-author-name">Dobó Máté</span> <span class="authorAux-mtmt"> elválasztástechnika, (királis HPLC)</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10100801"><a href="/gui2/?type=authors&mode=browse&sel=10100801" target="_blank">Molnár Gergely (<span class="authorship-author-name">Molnár Gergely</span> <span class="authorAux-mtmt"> Királis nagyhatékonyságú folyadékkromatográfia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10016687"><a href="/gui2/?type=authors&mode=browse&sel=10016687" target="_blank">Rozmer Zsuzsanna (<span class="authorship-author-name">Rozmer Zsuzsanna</span> <span class="authorAux-mtmt"> Gyógyszerészi kémia</span>) </a> </span> <span class="author-affil"><span title="Pécsi Tudományegyetem">PTE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Szabó Zoltán-István </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040691"><a href="/gui2/?type=authors&mode=browse&sel=10040691" target="_blank">Fiser Béla (<span class="authorship-author-name">Fiser Béla</span> <span class="authorAux-mtmt"> Számítógépes kémia, Anyagtudomány</span>) </a> </span> <span class="author-affil"><span title="Miskolci Egyetem">ME</span>/<span title="Anyag- és Vegyészmérnöki Kar">AVK</span>/Kémiai Intézet; <span title="II. Rákóczi Ferenc Kárpátaljai Magyar Főiskola ">II.RFKMF</span>/Biológia és Kémia Tanszék; <span title="Miskolci Egyetem">ME</span>/<span title="Karon kívüli szervezeti egységek">K</span>/Korszerű Anyagok és Intelligens Technológiák Felsőoktatási és Ipari Együttműködési Központ</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10028950"><a href="/gui2/?type=authors&mode=browse&sel=10028950" target="_blank">Tóth Gergő ✉ (<span class="authorship-author-name">Tóth Gergő</span> <span class="authorAux-mtmt"> gyógyszerkémia/analitika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Gyógyszerésztudományi Kar">GYTK</span>/Gyógyszerészi Kémiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Központi Szervezeti Egységek (beleértve az Egyéb Szervezeti Egységeket)">KSZE</span>/Farmakológiai és Gyógyszerkutatás-fejlesztési Központ</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;35429645" target="_blank">Enantioselective Binding of Proton Pump Inhibitors to Alpha1-Acid Glycoprotein and Human Serum Albumin—A Chromatographic, Spectroscopic, and In Silico Study</a></div> <div> <span class="journal-title">INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1661-6596">1661-6596</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1422-0067">1422-0067</a>)</span>: <span class="journal-volume">25</span> <span class="journal-issue">19</span> <span class="page"> Paper 10575. 20 p. </span> <span class="year">(2024)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.3390/ijms251910575" target="_blank" href="https://doi.org/10.3390/ijms251910575"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="001332332800001" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/001332332800001"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85206447331" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85206447331"> Scopus </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="https://dspace.kmf.uz.ua/jspui/handle/123456789/4589" target="_blank" href="https://dspace.kmf.uz.ua/jspui/handle/123456789/4589"> Repozitóriumban </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="39408903" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=39408903&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Organic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Spectroscopy&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Computer Science Applications&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Inorganic Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Physical and Theoretical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Catalysis&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 10</span> | Független: 8 | Függő: 2 | Nem jelölt: 0 | WoS jelölt: 9 | Scopus jelölt:&nbsp;9 | WoS/Scopus jelölt:&nbsp;10 | DOI jelölt:&nbsp;10 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;35429645&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 35429645</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="funder"> (FK 146930), (ÚNKP-23-3-II-SE-3), (ÚNKP-23-2-III-SE-6), (EKÖP-2024-57) </div> <div class="lastModified">Utolsó módosítás: 2026.05.19. 09:33 Miskolczi Éva (SE_KK_Admin5_DME, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: National Research, Development, and Innovation Office, Hungary [NKFIH FK 146930]; National Research, Development, and Innovation fund; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; National Research, Development, and Innovation Fund (Hungary) [TKP2021-NVA-14]; [UNKP-23-3-II-SE-3]; [UNKP-23-2-III-SE-6]; [EKOEP-2024-57]; [UNKP-2023-3-...</pre> </div></div>