Activated THP-1 Macrophage-Derived Factors Increase the Cytokine, Fractalkine, and EGF Secretions, the Invasion-Related MMP Production, and Antioxidant Activity of HEC-1A Endometrium Cells

Pandur, Edina ✉ [Pandur, Edina (Gyógyszerészi bio...), szerző] Gyógyszerészi Biológiai Tanszék (PTE / GYTK); Humán Reprodukciós Nemzeti Laboratórium (PTE); Pap, Ramóna [Pap, Ramóna (Gyógyszerészi bio...), szerző] Gyógyszerészi Biológiai Tanszék (PTE / GYTK); Humán Reprodukciós Nemzeti Laboratórium (PTE); Sipos, Katalin [Sipos, Katalin (Expressziós analí...), szerző] Gyógyszerészi Biológiai Tanszék (PTE / GYTK); Humán Reprodukciós Nemzeti Laboratórium (PTE)

Angol nyelvű Szakcikk (Folyóiratcikk) Tudományos
Megjelent: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 1661-6596 1422-0067 25 (17) Paper: 9624 , 20 p. 2024
  • SJR Scopus - Inorganic Chemistry: D1
Azonosítók
Támogatások:
  • (Recovery and Resilience Facility of the European Union within the framework of Programme Széchenyi Plan Plus)
  • (RRF-2.3.1-21-2022-00012)
Endometrium receptivity is a multifactor-regulated process involving progesterone receptor-regulated signaling, cytokines and chemokines, and additional growth regulatory factors. In the female reproductive system, macrophages have distinct roles in the regulation of receptivity, embryo implantation, immune tolerance, and angiogenesis or oxidative stress. In the present study, we investigated the effects of PMA-activated THP-1 macrophages on the receptivity-related genes, cytokines and chemokines, growth regulators, and oxidative stress-related molecules of HEC-1A endometrium cells. We established a non-contact co-culture in which the culture medium of the PMA-activated macrophages exhibiting the pro-inflammatory phenotype was used for the treatment of the endometrial cells. In the endometrium cells, the expression of the growth-related factors activin and bone morphogenetic protein 2, the growth hormone EGF, and the activation of the downstream signaling molecules pERK1/2 and pAkt were analyzed by ELISA and Western blot. The secretions of cytokines and chemokines, which are involved in the establishment of endometrial receptivity, and the expression of matrix metalloproteinases implicated in invasion were also determined. Based on the results, the PMA-activated THP-1 macrophages exhibiting a pro-inflammatory phenotype may play a role in the regulation of HEC-1A endometrium cells. They alter the secretion of cytokines and chemokines, as well as the protein level of MMPs of HEC-1A cells. Moreover, activated THP-1 macrophages may elevate oxidative stress protection of HEC-1A endometrium cells. All these suggest that pro-inflammatory macrophages have a special role in the regulation of receptivity-related and implantation-related factors of HEC-1A cells.
Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
2024-11-11 05:18